Abstract: Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.
Type:
Grant
Filed:
October 25, 2012
Date of Patent:
December 29, 2015
Assignees:
Sangamo BioSciences, Inc., The Regents of the University of California
Inventors:
Gregory J. Cost, Michael C. Holmes, Noriyuki Kasahara, Josee Laganiere, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Fyodor Urnov, Lei Zhang
Abstract: Disclosed herein are methods and compositions for inactivation of the human glucocorticoid receptor (GR) gene by targeted cleavage of genomic DNA encoding the GR. Such methods and compositions are useful, for example, in therapeutic applications which require retention of immune function during glucocorticoid treatment.
Type:
Grant
Filed:
November 13, 2007
Date of Patent:
December 22, 2015
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Andreas Reik, Michael Jensen, Michael C. Holmes, Philip D. Gregory, Dale Ando
Abstract: Disclosed herein are methods and compositions for genome editing of one or more loci in a rat, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
Abstract: Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences.
Abstract: Disclosed herein are zinc fingers comprising CCHC zinc coordinating residues. Also described are zinc finger proteins and fusion proteins comprising these CCHC zinc fingers as well as polynucleotides encoding these proteins. Methods of using these proteins for gene editing and gene regulation are also described.
Inventors:
Qihua C. Cai, Vipula K. Shukla, Joseph F. Petolino, Lisa W. Baker, Robbi J. Garrison, Ryan C. Blue, Jon C. Mitchell, Nicole L. Arnold, Sarah E. Worden, Jeffrey Miller, Fyodor Urnov
Abstract: Disclosed herein are methods and compositions for insertion of Factor IX (FIX) sequences into the genome of a cell for treating hemophilia B.
Type:
Grant
Filed:
October 12, 2011
Date of Patent:
November 3, 2015
Assignees:
Sangamo BioSciences, Inc., The Children's Hospital of Philadelphia
Inventors:
Philip D. Gregory, Katherine A. High, Michael C. Holmes, Hojun Li
Abstract: Disclosed herein are compositions for linking DNA binding modules to allow for specific and selective binding to module subsites separated by 1 or more base pairs. Also described are methods of making and using compositions comprising these linkers.
Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
Abstract: Nucleases and methods of using these nucleases for expressing a transgene from a safe harbor locus in a secretory tissue, and clones and animals derived therefrom.
Abstract: Disclosed herein are methods and compositions for rapidly identifying active nucleases and cells having nuclease-mediated genomic modifications.
Type:
Grant
Filed:
September 15, 2011
Date of Patent:
September 1, 2015
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Michael C. Holmes, Yannick Doyon, Tianjian Li
Abstract: Disclosed herein are methods and compositions for rapidly identifying active nucleases and cells having nuclease-mediated genomic modifications.
Type:
Grant
Filed:
March 11, 2013
Date of Patent:
August 25, 2015
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Yannick Doyon, Michael C. Holmes, Tianjian Li
Abstract: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for integration of an exogenous sequence into a specified region of interest in the genome of a cell.
Type:
Grant
Filed:
June 16, 2011
Date of Patent:
June 2, 2015
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Russell DeKelver, Philip D. Gregory, Michael C. Holmes, Fyodor Urnov
Abstract: Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
Type:
Grant
Filed:
July 8, 2011
Date of Patent:
April 14, 2015
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Gregory J. Cost, Dmitry M. Guschin, Fyodor Urnov
Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
Inventors:
Noel Cogan, John Forster, Matthew Hayden, Tim Sawbridge, German Spangenberg, Steven R. Webb, Manju Gupta, William Michael Ainley, Matthew J. Henry, Jeffrey C. Miller, Dmitry Y. Guschin
Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
Abstract: Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.
Type:
Grant
Filed:
November 10, 2010
Date of Patent:
February 17, 2015
Assignees:
Sangamo BioSciences, Inc., Ospedale San Raffaele S.R.L.
Inventors:
Maria Chiara Bonini, Pietro Genovese, Philip D. Gregory, Michael C. Holmes, Luigi Naldini, David Paschon, Elena Provasi, Lei Zhang