Patents Assigned to Sangamo Biosciences, Inc.
  • Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression
    Patent number: 8772025
    Abstract: Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
    Type: Grant
    Filed: January 27, 2011
    Date of Patent: July 8, 2014
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Trevor Collingwood, Gregory J. Cost
  • ENGINEERED ZINC FINGER PROTEINS TARGETING PLANT GENES INVOLVED IN FATTY ACID BIOSYNTHESIS
    Publication number: 20140157458
    Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.
    Type: Application
    Filed: November 25, 2013
    Publication date: June 5, 2014
    Applicants: Sangamo BioSciences, Inc., Dow AgroSciences LLC
    Inventors: Russell DeKelver, Manju Gupta, Jeffrey C. Miller, Stephen Novak, Joseph F. Petolino
  • Modulation of stem cells using zinc finger proteins
    Patent number: 8735153
    Abstract: Methods and compositions for modifying stem cells using one or more ZFPs are disclosed. Such methods and compositions are useful for facilitating processes such as, for example, dedifferentiating cells, differentiating stems cells into the desired phenotype, propagating stem cells and/or facilitating cloning.
    Type: Grant
    Filed: September 24, 2002
    Date of Patent: May 27, 2014
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Michael Moore, Timothy Farries, Trevor Collingwood, Fyodor Urnov, Elizabeth J. Wolffe
  • NOVEL DNA-BINDING PROTEINS AND USES THEREOF
    Publication number: 20140134723
    Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
    Type: Application
    Filed: October 28, 2013
    Publication date: May 15, 2014
    Applicant: Sangamo BioSciences, Inc.
    Inventors: Philip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
  • NOVEL DNA-BINDING PROTEINS AND USES THEREOF
    Publication number: 20140134741
    Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
    Type: Application
    Filed: October 28, 2013
    Publication date: May 15, 2014
    Applicant: Sangamo Biosciences, Inc.
    Inventors: Philip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
  • NOVEL DNA-BINDING PROTEINS AND USES THEREOF
    Publication number: 20140134740
    Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
    Type: Application
    Filed: October 28, 2013
    Publication date: May 15, 2014
    Applicant: Sangamo BioSciences, Inc.
    Inventors: Philip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
  • METHODS AND COMPOSITIONS FOR GENE INACTIVATION
    Publication number: 20140127811
    Abstract: Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
    Type: Application
    Filed: October 15, 2013
    Publication date: May 8, 2014
    Applicant: Sangamo BioSciences, Inc.
    Inventors: Dale Ando, Michael C. Holmes, Gary Ka Leong Lee
  • ENGINEERED CLEAVAGE HALF-DOMAINS
    Publication number: 20140120612
    Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
    Type: Application
    Filed: January 2, 2014
    Publication date: May 1, 2014
    Applicant: SANGAMO BIOSCIENCES, INC.
    Inventors: Yannick Doyon, Jeffrey C. Miller
  • Methods and compositions for targeted single-stranded cleavage and targeted integration
    Patent number: 8703489
    Abstract: Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences.
    Type: Grant
    Filed: August 18, 2009
    Date of Patent: April 22, 2014
    Assignee: Sangamo BioSciences, Inc.
    Inventor: Jianbin Wang
  • METHODS AND COMPOSITIONS FOR TARGETED CLEAVAGE AND RECOMBINATION
    Publication number: 20140086885
    Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
    Type: Application
    Filed: March 15, 2013
    Publication date: March 27, 2014
    Applicant: C/O SANGAMO BIOSCIENCES, INC.
    Inventor: C/O SANGAMO BIOSCIENCES, INC.
  • Zinc finger proteins for DNA binding and gene regulation in plants
    Patent number: 8680021
    Abstract: Disclosed herein are modified plant zinc finger proteins; compositions comprising modified plant zinc finger proteins and methods of making and using modified plant zinc finger proteins. The modified plant zinc finger proteins, in contrast to naturally-occurring plant zinc finger proteins, have a binding specificity that is determined by tandem arrays of modular zinc finger binding unit.
    Type: Grant
    Filed: August 28, 2006
    Date of Patent: March 25, 2014
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Andrew Jamieson, Guofu Li
  • TARGETED DELETION OF CELLULAR DNA SEQUENCES
    Publication number: 20140065667
    Abstract: Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a cell such that two targeted cleavage events occur. Subsequent cellular repair mechanisms result in deletion of sequences between the two cleavage sites.
    Type: Application
    Filed: March 4, 2013
    Publication date: March 6, 2014
    Applicant: SANGAMO BIOSCIENCES, INC.
    Inventor: SANGAMO BIOSCIENCES, INC.
  • METHODS AND COMPOSITIONS FOR MODULATING PD1
    Publication number: 20140030240
    Abstract: Disclosed herein are methods and compositions for modulating expression of a PD1 gene.
    Type: Application
    Filed: September 27, 2013
    Publication date: January 30, 2014
    Applicant: Sangamo BioSciences, Inc.
    Inventors: Philip D. Gregory, Michael C. Holmes, Matthew C. Mendel, Xiangdong Meng, David Paschon, Andreas Reik, Fyodor Urnov
  • Engineered cleavage half-domains
    Patent number: 8623618
    Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
    Type: Grant
    Filed: December 30, 2011
    Date of Patent: January 7, 2014
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Yannick Doyon, Jeffrey C. Miller
  • METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION OF TRANSGENES
    Publication number: 20130326645
    Abstract: Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.
    Type: Application
    Filed: May 7, 2013
    Publication date: December 5, 2013
    Applicants: Dow AgroSciences LLC, Sangamo BioSciences, Inc.
    Inventors: Gregory J. Cost, Fyodor Urnov, William M. Ainley, Joseph F. Petolino, Jayakumar Pon Samuel, Steven R. Webb, Lakshmi Sastry-Dent
  • Methods and compositions for generation of Bax-and Bak-deficient cell lines
    Patent number: 8597912
    Abstract: Disclosed herein are methods and compositions for generation of Bak- and/or Bax-deficient cell lines using engineered nucleases.
    Type: Grant
    Filed: June 10, 2009
    Date of Patent: December 3, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Trevor Collingwood, Philip D. Gregory
  • Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis
    Patent number: 8592645
    Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.
    Type: Grant
    Filed: October 22, 2010
    Date of Patent: November 26, 2013
    Assignees: Dow AgroSciences LLC, Sangamo BioSciences, Inc.
    Inventors: Russell DeKelver, Manju Gupta, Jeffrey C. Miller, Stephen Novak, Joseph F. Petolino
  • DNA-binding proteins and uses thereof
    Patent number: 8586526
    Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: November 19, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Philip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
  • Methods and compositions for gene inactivation
    Patent number: 8569253
    Abstract: Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
    Type: Grant
    Filed: May 9, 2011
    Date of Patent: October 29, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Dale Ando, Michael C. Holmes, Gary Ka Leong Lee
  • Methods and compositions for modulating PD1
    Patent number: 8563314
    Abstract: Disclosed herein are methods and compositions for modulating expression of a PD1 gene.
    Type: Grant
    Filed: November 17, 2010
    Date of Patent: October 22, 2013
    Assignee: Sangamo BioSciences, Inc.
    Inventors: Philip D. Gregory, Michael C. Holmes, Matthew C. Mendel, Xiangdong Meng, David Paschon, Andreas Reik, Fyodor Urnov