Patents Assigned to Sekisui Medical Co., Ltd.
  • Publication number: 20230183674
    Abstract: Provided is a method for purifying a nucleic acid capable of easily purifying a nucleic acid and efficiently recovering the nucleic acid. The method for purifying a nucleic acid including a step of bringing a sample containing a nucleic acid into contact with an extraction solution containing a metal cation to prepare an extraction solution containing the nucleic acid, a step of bringing the extraction solution containing the nucleic acid into contact with an anionic adsorbent 4 to cause the nucleic acid to adsorb to the anionic adsorbent 4, a step of bringing a wash solution having a pH of 5.0 or less into contact with the anionic adsorbent 4 to wash the anionic adsorbent 4 to which the nucleic acid is adsorbed, and a step of bringing a recovery solution having a pH of 6.0 or more into contact with the anionic adsorbent 4 to isolate the nucleic acid from the anionic adsorbent 4.
    Type: Application
    Filed: May 19, 2021
    Publication date: June 15, 2023
    Applicants: SEKISUI MEDICAL CO., LTD., SEKISUI CHEMICAL CO., LTD.
    Inventors: Sou YAMAGUCHI, Katsura UCHIDA, Takamasa KOUNO, Tatsunori TAKAMATSU, Takuya KINOSHITA
  • Publication number: 20230175067
    Abstract: It is intended to provide a rapid, convenient, and highly accurate method for determining the prognosis of cancer. The present invention provides a method for determining a tissue having renal cell carcinoma, comprising: (1) subjecting sample DNA to ion exchange chromatography, wherein the sample DNA is obtained by treating target genomic DNA prepared from a renal tissue of a subject with bisulfite, followed by PCR amplification; (2) calculating a derivative value of a detection signal of the chromatography; and (3) determining the renal tissue as being a tissue having renal cell carcinoma having poor prognosis when the derivative value calculated in the step (2) has two or more maximums.
    Type: Application
    Filed: July 14, 2022
    Publication date: June 8, 2023
    Applicants: NATIONAL CANCER CENTER, SEKISUI MEDICAL CO., LTD.
    Inventors: Yae KANAI, Eri ARAI, Yuriko KEMOTO, Takuya YOTANI
  • Patent number: 11668708
    Abstract: A method for avoiding the influence of a blood sample on a measurement error in a latex agglutination immunoassay. The measurement error caused by a blood sample in a latex agglutination immunoassay can be reduced by a method which includes a step of bringing the sample into contact, in a liquid phase, with latex particles carrying a substance having a specific affinity for an analyte in the presence of imidazole.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: June 6, 2023
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Kengo Fujimura, Junichi Kondou, Mitsuaki Yamamoto
  • Publication number: 20230158505
    Abstract: A specimen collection container is provided, the specimen collection container being capable of suppressing a change in a mixing ratio of a specimen collected to a liquid contained even when a long period of time has elapsed after manufacturing. A specimen collection container according to the present invention is a specimen collection container into which a specimen is collected, the specimen collection container including: a container main body having an opening; a plug attached to the opening; a barrier film disposed on an outer surface of the container main body; and a liquid contained in the container main body, the barrier film having a water vapor transmission rate at 40° C. and 90% RH of 0.8 g/(m2·day) or less.
    Type: Application
    Filed: March 23, 2021
    Publication date: May 25, 2023
    Applicants: SEKISUI MEDICAL CO., LTD., TOKUYAMA SEKISUI CO., LTD.
    Inventors: Takaya UCHIYAMA, Kuniya KOMAI, Masatoshi NIUNOYA, Yuuki GOTOU, Hironobu ISOGAWA
  • Publication number: 20230152335
    Abstract: Provided is a method for accurately measuring coagulation time of blood samples showing various coagulation reactions. The method for measuring blood coagulation time includes measuring coagulation reaction of a sample prepared by mixing a subject specimen and a coagulation time measurement reagent; calculating weighted average time of a calculation target area of a waveform related to coagulation velocity from the resulting measurement data; and determining the weighted average time as blood coagulation time.
    Type: Application
    Filed: March 5, 2021
    Publication date: May 18, 2023
    Applicant: SEKISUI MEDICAL CO., LTD.
    Inventor: Toshiki KAWABE
  • Publication number: 20230133626
    Abstract: Provided is a storage container for cell-containing solutions, which makes it possible to enhance the storage stability of cfDNA contained in a cell-containing solution even when the cell-containing solution is stored under a room temperature environment. The storage container for cell-containing solutions according to the present invention is intended to be used for storing a predetermined amount of a cell-containing solution, the storage container being provided with a container main body and a preservative solution contained in the container main body, in which the storage container has a first configuration such that the preservative solution contains a cell-membrane-permeable compound that has a molecular weight of 100 or less and cannot be frozen at 0° C.
    Type: Application
    Filed: March 3, 2021
    Publication date: May 4, 2023
    Applicants: SEKISUI MEDICAL CO., LTD., TOKUYAMA SEKISUI CO., LTD.
    Inventors: Yuuki GOTOU, Hironobu ISOGAWA, Takaya UCHIYAMA, Tomonori INOUE
  • Publication number: 20230136517
    Abstract: An optical measurement device 1 includes: a measurement unit; and a determination unit, wherein the determination unit calculates a difference value between the initial measurement value and the latter measurement value when the measurement value exceeds the first threshold value after the sample is held by the lateral flow test strip, determines that the sample is positive when the difference value exceeds a second threshold value set in advance, and determines that the sample is negative when the difference value is the second threshold value or less.
    Type: Application
    Filed: March 23, 2021
    Publication date: May 4, 2023
    Applicants: SEKISUI MEDICAL CO., LTD., HAMAMATSU PHOTONICS K.K.
    Inventors: Motoki MORITA, Keigo KOHNO, Daichi SUSUKI, Fusanori KONDO
  • Publication number: 20230118882
    Abstract: Disclosed is a sensitized magnetic responsive particle including: a magnetic responsive particle having a core particle and at least one magnetic layer disposed on the core particle, the magnetic layer containing microparticles of a magnetic metal and/or an oxide thereof; and a substance that specifically interacts with an analyte, the substance being supported on the magnetic responsive particle, wherein, assuming that a volume and a weight of the core particle are respectively vc and wc, and that a volume and a weight of the magnetic responsive particle are respectively ve and we, the magnetic material density [(we?wc)/(ve?vc)] satisfies the following expression 1: 2.0?(we?wc)/(ve?vc) ??Expression 1 The magnetic responsive particle has a high magnetic collection property in spite of a small particle size. When the magnetic responsive particle is used, a reagent for an immunoassay having excellent magnetic separability and capable of realizing high sensitivity can be provided.
    Type: Application
    Filed: March 24, 2021
    Publication date: April 20, 2023
    Applicant: SEKISUI MEDICAL CO., LTD.
    Inventors: Maasa YAJI, Yuya INABA, Shinichiro KITAHARA, Takeshi WAKIYA, Mai YAMAGAMI, Shuhei OBINATA
  • Patent number: 11614446
    Abstract: To provide an immunochromatographic test device capable of accurate diagnosis even when an excess sample is introduced. Provided is the immunochromatographic test device consisting: a test strip; a lower housing including a plurality of support bases that support the test strip; and an upper housing including a dropping hole for dropping a sample into the test strip and a detection window in a direction in which the sample introduced from the dropping hole develops on the test strip, wherein a width of the support base that supports portion of the test strip exposed from the detection window is smaller than a width of the test strip, or wherein among the plurality of support bases, the width of the support base arranged on the lower housing between a position corresponding to the detection window and a position corresponding to the dropping hole is larger than the width of the test strip.
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: March 28, 2023
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Kanako Azuma, Shinya Okuyama, Kazunori Saito, Keigo Kohno, Motoki Morita
  • Publication number: 20230090926
    Abstract: It is an object of the present disclosure to provide a method for reducing the influence of baseline disturbances in a method for detecting a target nucleic acid in dry blood filter paper by real-time PCR using a fluorescent dye, with a simple method. The present disclosure provides a method for detecting a target nucleic acid in dry blood filter paper by real-time PCR, the method including: (1) amplifying the target nucleic acid in the dry blood filter paper by applying thermal cycles to a sample solution containing a dry blood filter paper punch piece and a PCR reagent, wherein the PCR reagent includes a fluorescently labeled probe; (2) optically detecting the fluorescence intensity of the sample solution for each of the thermal cycles; and (3) performing quantitative analysis of the target nucleic acid using data after a predetermined number of cycles of the optically detected data.
    Type: Application
    Filed: February 19, 2021
    Publication date: March 23, 2023
    Applicant: SEKISUI MEDICAL CO., LTD.
    Inventors: Hiroyuki EBINUMA, Ikumi SUZUKI
  • Publication number: 20230090675
    Abstract: Provided is a blood collection container with which a specimen with a high leukocyte recovery rate and a small amount of contaminating erythrocytes can be obtained. A blood collection container according to the present invention is a blood collection container by which a predetermined amount of blood is collected, the blood collection container including: a blood collection container main body; and a hemocyte separation material contained in the blood collection container main body, the hemocyte separation material having a specific gravity at 25° C. of 1.075 or more and 1.
    Type: Application
    Filed: March 11, 2021
    Publication date: March 23, 2023
    Applicants: SEKISUI MEDICAL CO., LTD., TOKUYAMA SEKISUI CO., LTD.
    Inventors: Kuniya KOMAI, Hironobu ISOGAWA
  • Patent number: 11591351
    Abstract: The purpose of the present invention is to provide a protecting group which improves the solubility of a compound having a functional group protected with the protecting group in an organic solvent and which is easily separated and purified after a reaction with avoiding solidification or insolubilization.
    Type: Grant
    Filed: September 25, 2020
    Date of Patent: February 28, 2023
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Shinya Yano, Yuki Wakasugi, Yosuke Iwanaga
  • Publication number: 20230050815
    Abstract: Provide is a blood collection container capable of suppressing the occurrence of blood clots involving bubbles when blood containing heparin is coagulated, and capable of suppressing the production of fibrin in serum after separation when blood containing heparin is separated into serum and blood clots. A blood collection container according to the present invention includes: a blood collection container main body having an opening at one end thereof and a closed bottom at the other end thereof; a serine protease disposed in the blood collection container main body; and a heparin neutralizing agent disposed in the blood collection container main body, wherein when a region in which the serine protease is disposed is defined as a first region, and a region in which the heparin neutralizing agent is disposed is defined as a second region, the second region includes a region present on an other end side with respect to an end on the other end side of the first region.
    Type: Application
    Filed: November 30, 2020
    Publication date: February 16, 2023
    Applicants: SEKISUI MEDICAL CO., LTD., TOKUYAMA SEKISUI CO., LTD.
    Inventors: Yuji SETOGUCHI, Hironobu ISOGAWA
  • Patent number: 11566074
    Abstract: Provided are a method of efficiently producing an sIL-2R antigen in an amount necessary for antibody generation, and a method of producing an anti-sIL-2R antibody using the antigen. Specifically, provided are a method of producing soluble interleukin-2 receptor, including culturing SCC-3 cells and recovering soluble interleukin-2 receptor from a culture of the cells, and a method of producing an anti-soluble interleukin-2 receptor antibody, including immunizing an animal with sIL-2R produced by the method.
    Type: Grant
    Filed: September 29, 2016
    Date of Patent: January 31, 2023
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Hiroyuki Ebinuma, Kengo Fujimura
  • Publication number: 20230025583
    Abstract: A problem to be solved by the invention is to improve the sensitivity of measurement of an analyte in a sample by a sandwich immunoassay. The sensitivity can be improved significantly in a sandwich immunoassay using a first antibody and a second antibody when one or both of the first antibody and the second antibody are a mixture of a monoclonal antibody recognizing a linear epitope and a monoclonal antibody recognizing a conformational epitope.
    Type: Application
    Filed: November 27, 2020
    Publication date: January 26, 2023
    Applicant: SEKISUI MEDICAL CO., LTD.
    Inventors: Tomohide ASAI, Yoshihiro TANAKA, Ikumi SUZUKI, Satoko SEO, Tomoyuki USUI, Tomo SHIMIZU, Osamu MIYAZAKI
  • Publication number: 20230003746
    Abstract: The present invention addresses the problem of providing a method for measuring 25-hydroxy vitamin D and a measurement reagent, the method and the reagent being based on a competitive immunoassay. The present invention provides a reagent for measuring 25-hydroxy vitamin D based on a competitive immunoassay, which includes at least the following composition: (1) a Vitamin D derivative represented by the chemical formula (I) and/or (II), (2) an anti-25 hydroxy vitamin D antibody. In addition, the present invention provides a reagent for measuring vitamin D based on a competitive latex turbidimetric immunoassay (competitive LTIA) in which, in particular, a vitamin D derivative or an anti-25-hydroxy vitamin D antibody is immobilized on latex.
    Type: Application
    Filed: November 20, 2020
    Publication date: January 5, 2023
    Applicant: SEKISUI MEDICAL CO., LTD.
    Inventors: Mitsuaki YAMAMOTO, Mieko OTA, Tomohisa NISHIO
  • Patent number: 11542287
    Abstract: Provided is an alkyldiphenylmethane protective agent, which can prevent solidification or insolubilization of a compound by protecting a functional group of the compound to achieve easy separation and purification after a reaction.
    Type: Grant
    Filed: November 27, 2020
    Date of Patent: January 3, 2023
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Shinya Yano, Kenta Saito, Hideki Kubota
  • Publication number: 20220386913
    Abstract: There is provided a blood collection container capable of suppressing the contamination of white blood cells into blood plasma. A blood collection container according to the present invention is a blood collection container into which a predetermined amount of blood is collected. The blood collection container includes a blood collection container main body, and a blood plasma separation material, an osmotic pressure regulator, and an anticoagulant contained in the blood collection container main body. The specific gravity of the blood plasma separation material at 25° C. is 1.030 or more and 1.120 or less. When the osmotic pressure regulator and the anticoagulant contained in the blood collection container main body are dissolved in an amount of physiological saline solution equivalent to a predetermined amount of blood collected in the blood collection container to obtain an osmotic pressure measurement solution, the specific gravity of the blood plasma separation material at 25° C.
    Type: Application
    Filed: December 2, 2020
    Publication date: December 8, 2022
    Applicants: SEKISUI MEDICAL CO., LTD., TOKUYAMA SEKISUI CO., LTD
    Inventor: Kuniya KOMAI
  • Publication number: 20220373565
    Abstract: An APTT prolongation factor estimation system includes one or more facilities each including an analyzer which measures a blood coagulation reaction of a subject blood specimen, a database which stores data on a blood coagulation reaction and APTT prolongation factor of blood specimens, and a computer which estimates an APTT prolongation factor of the subject blood specimen based on the data on the blood coagulation reaction from the analyzer and the data stored in the database. The facilities each further includes a data transmission unit which transmits, to the computer, the data on the blood coagulation reaction of the subject blood specimen obtained by the analyzer, and a data reception unit which receives a result of the estimation of the APTT prolongation factor, which is obtained by the computer, regarding the subject blood specimen measured by each of the analyzers.
    Type: Application
    Filed: June 19, 2020
    Publication date: November 24, 2022
    Applicants: SEKISUI MEDICAL CO., LTD., PUBLIC UNIVERSITY CORPORATION NARA MEDICAL UNIVERSITY
    Inventors: Toshiki KAWABE, Yukio ODA, Midori SHIMA, Keiji NOGAMI, Kenichi OGIWARA, Naruto SHIMONISHI
  • Patent number: 11485747
    Abstract: An object of the present invention is to develop a protecting group, which can prevent solidification or insolubilization of a compound by protecting a functional group to achieve easy separation and purification after a reaction. A diphenylmethane compound represented by general formula (1): wherein Y represents —OR19 (wherein R19 represents a hydrogen atom or an active ester-type protecting group), —NHR20 (wherein R20 represents, for example, a hydrogen atom, a C1-6 linear or branched alkyl group, or an aralkyl group, at least one of R1 to R10 represents a group represented by formula (2): —O—R11—X-A??(2) and the others each independently represent a hydrogen atom, a halogen atom, a C1-4 alkyl group, or a C1-4 alkoxy group; R11 represents a C1-16 linear or branched alkylene group; X represents O or CONR21 (wherein R21 represents a hydrogen atom, or a C1-4 alkyl group; and A represents, for example, a group represented by formula (3).
    Type: Grant
    Filed: October 20, 2020
    Date of Patent: November 1, 2022
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Shinya Yano, Hideki Kubota