Patents Assigned to Sigma-Aldrich Co. LLC
  • Patent number: 9671393
    Abstract: The present invention provides isolated, non-viable bacterial cells comprising a plurality of nucleic acid crosslinks, methods for making said cells, and methods for using said cells to isolate DNA-protein complexes. In particular, the nucleic acids of the cells are crosslinked by contacting the cells with a furocoumarin compound and ultraviolet light.
    Type: Grant
    Filed: September 2, 2011
    Date of Patent: June 6, 2017
    Assignee: SIGMA-ALDRICH CO., LLC
    Inventors: Vikas B. Palhan, Carol Kreader, Ernie Mueller
  • Publication number: 20170051354
    Abstract: The present disclosure provides genetically engineered cell lines comprising chromosomally integrated synthetic sequences having predetermined epigenetic modifications, wherein a predetermined epigenetic modification is correlated with a known diagnosis, prognosis or level of sensitivity to a disease treatment. Also provided are kits comprising said epigenetically modified synthetic nucleic acids or cells comprising said epigenetically modified synthetic nucleic acids that can be used as reference standards for predicting responsiveness to therapeutic treatments, diagnosing diseases, or predicting disease prognosis.
    Type: Application
    Filed: April 24, 2015
    Publication date: February 23, 2017
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Gregory D. DAVIS, Qiaohua KANG
  • Publication number: 20160376582
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Application
    Filed: November 25, 2014
    Publication date: December 29, 2016
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventor: Carol KREADER
  • Patent number: 9528143
    Abstract: A composition suitable for formulation of an enzymatic reaction mixture, the composition comprising a reaction component essential for an ex-vivo non-polymerase enzymatic reaction in which a substrate is catalyzed by an enzyme in a reaction mixture to form a product, and a tracer compatible with the enzyme, the composition being substantially free of the substrate.
    Type: Grant
    Filed: April 12, 2007
    Date of Patent: December 27, 2016
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Brian W. Ward, David M. Ornitz, Michael G. Deines, Thomas F. Bittick
  • Patent number: 9512444
    Abstract: The present invention provides methods and kits for editing specific chromosomal sequences in cells. In particular, targeting endonucleases and single-stranded nucleic acids are used to edit the chromosomal sequence.
    Type: Grant
    Filed: July 22, 2011
    Date of Patent: December 6, 2016
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Fuqiang Chen, Shondra M. Pruett-Miller, Gregory D. Davis
  • Patent number: 9376484
    Abstract: The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.
    Type: Grant
    Filed: January 10, 2013
    Date of Patent: June 28, 2016
    Assignee: SIGMA-ALDRICH CO. LLC
    Inventors: Nan Lin, Natalie Sealover, Henry George, Kevin Kayser
  • Publication number: 20160145645
    Abstract: The present disclosure encompasses an isolated cell comprising an exogenous nucleic acid sequence located within or proximal to a predetermined genomic locus, wherein the exogenous nucleic acid sequence comprises at least one recognition sequence which can be exploited by one or more polynucleotide modification enzymes for targeted integration of a recombinant protein. The disclosure further provides methods for preparing such cells, and methods for retargeting such cells for the production of recombinant proteins, and kits for the same.
    Type: Application
    Filed: June 19, 2014
    Publication date: May 26, 2016
    Applicant: Sigma-Aldrich Co. LLC
    Inventors: Scott BAHR, Trissa BORGSCHULTE, Kevin KAYSER
  • Patent number: 9297071
    Abstract: A solid precursor delivery assembly generally includes a container having upper and lower portions and chambers defined within the container, including an inlet chamber, an outlet chamber, and first and second precursor chambers. The first and second precursor chambers are configured for holding precursor material within the container. Sintered frits are fixedly coupled and sealed to an interior portion of the container, and define at least part of the chambers within the container. At least one of the sintered frits is configured for retaining precursor material thereon within the first precursor chamber, and at least one of the sintered frits is configured for retaining precursor material thereon within the second precursor chamber. An inlet is coupled to the container for delivering carrier gas into the container, and an outlet is coupled to the container for use in removing vaporized precursor material and carrier gas from the container.
    Type: Grant
    Filed: October 21, 2010
    Date of Patent: March 29, 2016
    Assignee: SIGMA-ALDRICH CO. LLC
    Inventors: Ravi Kanjolia, Chris Platts, Nam Nguyen, Mark Wilkinson
  • Publication number: 20160017366
    Abstract: The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.
    Type: Application
    Filed: December 5, 2013
    Publication date: January 21, 2016
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Fuqiang CHEN, Gregory D. DAVIS, Qiaohua KANG, Scott W. KNIGHT
  • Patent number: 9206404
    Abstract: Disclosed herein are methods and compositions for genome editing of one or more loci in a rat, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
    Type: Grant
    Filed: December 3, 2009
    Date of Patent: December 8, 2015
    Assignees: Sangamo BioSciences, Inc., Sigma-Aldrich Co. LLC
    Inventors: Xiaoxia Cui, Aron M. Geurts, Fyodor Urnov
  • Patent number: 9175023
    Abstract: Molybdenum complexes and use thereof in thin film deposition, such as CVD and ALD are provided herein. The molybdenum complexes correspond in structure to Formula (I) and Formula (II), wherein R1, R3, R5, R7, R8 and R10 are independently and at each occurrence alkyl; R2, R6 and R9 are independently alkyl; R4 and R11 are independently and at each occurrence selected from the group consisting of alkyl, alkenyl, and alkynyl; x, z, a, c, d and f are independently zero, 1, or 2; y, b and e are independently zero or 1; and n and m are independently zero to 5.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: November 3, 2015
    Assignee: SIGMA-ALDRICH CO. LLC
    Inventors: Rajesh Odedra, Shaun Garratt, Mark Saly, Ravi Kanjolia
  • Patent number: 9168507
    Abstract: This disclosure provides methods and devices for quantitating, separating and/or detecting water in a liquid, gas or solid sample comprising one or more chemicals, the method comprising: providing the liquid, gas or solid sample comprising water and the one or more chemicals; and exposing said liquid, gas or solid sample to at least one solid support including at least one dicationic and/or tricationic species of Formula I or II adsorbed, absorbed or immobilized on the solid support.
    Type: Grant
    Filed: September 24, 2012
    Date of Patent: October 27, 2015
    Assignee: SIGMA-ALDRICH CO., LLC
    Inventor: Daniel W. Armstrong
  • Patent number: 9062303
    Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
    Type: Grant
    Filed: June 22, 2010
    Date of Patent: June 23, 2015
    Assignee: SIGMA-ALDRICH CO. LLC
    Inventors: Fuqiang Chen, Carol Kreader
  • Patent number: 9062338
    Abstract: The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: June 23, 2015
    Assignee: SIGMA-ALDRICH CO. LLC
    Inventors: Nan Lin, Henry J. George, Joaquina Mascarenhas, Trevor N. Collingwood, Kevin J. Kayser, Katherine Achtien
  • Publication number: 20150152463
    Abstract: The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.
    Type: Application
    Filed: January 30, 2015
    Publication date: June 4, 2015
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Nan Lin, Henry J. George, Joaquina Mascarenhas, Trevor N. Collingwood, Kevin J. Kayser, Katherine Achtien
  • Patent number: 9028917
    Abstract: A method for forming a cobalt-containing thin film by a vapor deposition process is provided. The method comprises using at least one precursor corresponding in structure to Formula (I); wherein R1 and R2 are independently C2-C8-alkyl; x is zero, 1 or 2; and y is zero or 1; wherein both x and y can not be zero simultaneously.
    Type: Grant
    Filed: July 27, 2010
    Date of Patent: May 12, 2015
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Rajesh Odedra, Neil Boag, Jeff Anthis, Ravi Kanjolia
  • Publication number: 20150079657
    Abstract: The present disclosure provides compositions and methods for acetylating histones at targeted chromosomal locations in a cell. In particular, the disclosure provides a fusion protein comprising a DNA binding domain and at least one histone acetyltransferase (HAT) domain, such that the DNA binding domain targets the fusion protein to a targeted chromosomal location and the HAT domain acetylates histones at the targeted location.
    Type: Application
    Filed: February 28, 2013
    Publication date: March 19, 2015
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Qingzhou Ji, Carol Kreader
  • Patent number: 8980643
    Abstract: Dicationic liquid salts and methods of using such dicationic liquid salts in techniques such as ESI-MS are provided.
    Type: Grant
    Filed: February 17, 2009
    Date of Patent: March 17, 2015
    Assignee: Sigma-Aldrich Co., LLC
    Inventor: Daniel W. Armstrong
  • Patent number: 8980583
    Abstract: The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: March 17, 2015
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Nan Lin, Henry J. George, Joaquina Mascarenhas, Trevor N. Collingwood, Kevin J. Kayser, Katherine Achtien
  • Patent number: 8927748
    Abstract: Organometallic complexes and use thereof in thin film deposition, such as CVD and ALD are provided herein. The organometallic complexes are (alkyl-substituted ?3-allyl)(carbonyl)metal complexes.
    Type: Grant
    Filed: August 8, 2012
    Date of Patent: January 6, 2015
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Rajesh Odedra, Neil Boag, Jeff Anthis, Ravi Kanjolia, Mark Saly