Abstract: Disclosed are methods for identifying molecular interactions between DNA sequences and proteins in vivo. The methods of the invention employ known or suspected DNA-binding proteins and genomic DNA in a plasmid library. Interacting molecules direct the expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs useful in practicing the methods of the invention.

Abstract: Cyclopropane fatty acid synthase genes and polypeptides are described. Plants are transformed with such genes to produce such polypeptides.

Abstract: Ablation of holes having diameters as small as 82 nm and having clean walls was obtained in a poly(methyl methacrylate) on a silicon substrate by focusing pulses from a Ne-like Ar, 46.9 nm wavelength, capillary-discharge laser using a freestanding Fresnel zone plate diffracting into third order is described. Spectroscopic analysis of light from the ablation has also been performed. These results demonstrate the use of focused coherent EUV/SXR light for the direct nanoscale patterning of materials.

Abstract: Permeability of a fluid through a saturated material is determined by measuring the dynamic response of that saturated material to shaking vibrations and/or shear wave propagation, and then mapping the dynamic response (preferably, viscoelastic stiffness and damping properties) to an invented model (called “KVMB”) that yields the property of permeability. The preferred embodiments may use shear waves, inertial effects, and/or transmission effects, but preferably not compression, to force fluids through the pores. The mapping preferably predicts two possible mappings to permeability, coupled and uncoupled. The preferred methods are both internally consistent and directly related to known laws of physics rather than dependent on empirical calibrations.

Abstract: The invention provides compositions comprising and methods employing methanobactin, as well as methanobactin synthetase sequences.

Abstract: A steering mechanism that operates by shifting weight in the desired direction. One embodiment includes an arm perpendicularly hinged to the underside of a platform. Connected to the bottom of the arm is a pivoting axle, which is in turn linked to a series of gears that lead back to the platform to form concurrent motion between the platform and axle. Supporting the platform in place is a pair of springs fastened to the arm on hinged mounts secured in place by a pair of stationary mounts fixed to the platform.

Abstract: A composition comprising PAA nanoparticles containing a post loaded tetrapyrollic photosensitizer and an imaging agent and methods for making and using same.

Abstract: A system and method of providing trusted service transactions includes associating a commitment with a remote service. The commitment includes a trusted list of runtime dependencies to execute a transaction. The method includes monitoring an actual list of runtime dependencies invoked during execution of the transaction using a trusted monitor. Execution is blocked if a deviation of the actual list from the trusted list is detected. Therefore, a completed transaction is allowed only if no deviation is found between the trusted and invoked list of runtime dependencies. A certificate authority in cooperation with software vendors preferably provide a signed commitment. The commitment is delivered by the provider to a user upon request and verified by the requester. The transaction is then executed by the user. Therefore, trust is verified before and during the transaction and privacy of data is guaranteed after completion.

Abstract: A family of PCR assays is disclosed for determining, both qualitatively and quantitatively, presence of material from a predetermined species source and for quantifying the amount of such material. The assays are based respectively on SINEs uniquely characteristic of pig species, cow species, chicken species, and ruminant sub-order, and having a high copy number. The assays disclosed permit rapid, inexpensive evaluation of meat samples to facilitate elimination from their diet of pork or beef by persons desiring to avoid such food sources; as well as the assay of cattle feed to determine presence therein of ruminant-source proteins, which are a potential source of bovine spongiform encephalopathy (BSE), commonly referred to as “mad cow disease.” The assays amplify the predetermined unique SINEs and the resulting amplified mixture is then evaluated qualitatively by electrophoresis on gel containing ethidium bromide or quantitatively by SYBR Green-based detection or TaqMan chemistry.

Abstract: Zn2+-chelating motif-tethered fatty acids as histone deacetylase (HDAC) inhibitors. Compounds performed well in in vitro and in vivo tests.

Abstract: The present invention provides novel compounds of formulas I-IX, as described herein. Also provided are compositions of compounds of formulas I-IX, methods of making compounds of formulas I-IX, and methods of using compounds of formulas I-IX. The compounds of the invention can be used to inhibit matrix metalloproteinases, and are useful to treat conditions and diseases associated therewith.

Abstract: A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.

Abstract: Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic Escherichia coli. The vaccine may include an Escherichia coli strain. The Escherichia coli strain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of Escherichia coli. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain of Escherichia coli with the genetic fusion.

Abstract: A composition and method for enhancing immune response in a living organism is disclosed. In particular, the present disclosure provides an adjuvant peptide for use in raising an immune response to an antigen. The adjuvant peptide is selected from a group of peptides with an HIV-related sequence. Additionally, the adjuvant peptide can comprise a fusion-protein that acts as a mucosal adjuvant. The adjuvant peptide can be transformed into one or more living cells, such that the mucosal adjuvant can be produced in living cells and then administered by systemic, mucosal or epidermal delivery.

Abstract: The present invention provides methods and compositions for enhancing memory in animals, including humans by the administration of an effective amount of an atypical form of protein kinase C such as protein kinase M zeta (PKM?) or protein kinase C iota/lambda.

Abstract: The present invention provides methods for reducing alcohol drinking behavior in humans and treating acute alcohol poisoning through the use of neutral or negative modulating agents of the neurosteroid sites on GABAA receptors. These agents avoid the unwanted side-effects of opiate antagonists and displays specificity toward the neurosteroid binding sites on GABAA receptors.

Abstract: A homopolymer of 1,4-benzodioxepin-3-cyclohexyl-2,5-dione with a Tg of 120° C. Copolymers are also described. The polymers are useful for surgical and other applications where biodegradability is important.

Abstract: Non-volatile memory devices with two stacked layers of chalcogenide materials comprising the active memory device have been investigated for their potential as phase change memories. The devices tested included GeTe/SnTe, Ge2Se3/SnTe, and Ge2Se3/SnSe stacks. All devices exhibited resistance switching behavior. The polarity of the applied voltage with respect to the SnTe or SnSe layer was critical to the memory switching properties, due to the electric field induced movement of either Sn or Te into the Ge-chalcogenide layer. One embodiment of the invention is a device comprising a stack of chalcogenide-containing layers which exhibit phase change switching only after a reverse polarity voltage potential is applied across the stack causing ion movement into an adjacent layer and thus “activating” the device to act as a phase change random access memory device or a reconfigurable electronics device when the applied voltage potential is returned to the normal polarity.

Abstract: A new reactant delivery system for delivering reactants to the membrane electrode assembly of a fuel cell. The invention uses a plurality of small holes to propel high-velocity streams of reactant gases (“microjets”) against an impingement plate. The microjets assist in catalyzing the reactant gases and forcing them toward the proton exchange membrane. Reactant gas flow is primarily perpendicular to the orientation of the proton exchange membrane, thereby enhancing diffusion rates. In addition, each microjet acts like an expansion valve, which significantly cools the flowing gas and provides internal heat absorption. This internal heat absorption permits higher energy densities in the fuel cell.

Abstract: There is a constant battle to break even between continuing improvements in DRAM capacities and the growing memory demands of large-memory high-performance applications. Performance of such applications degrades quickly once the system hits the physical memory limit and starts swapping to the local disk. We present the design, implementation and evaluation of Anemone—an Adaptive Network Memory Engine—that virtualizes the collective unused memory of multiple machines across a gigabit Ethernet LAN, without requiring any modifications to the either the large memory applications or the Linux kernel. We have implemented a working prototype of Anemone and evaluated it using real-world unmodified applications such as ray-tracing and large in-memory sorting. Our results with the Anemone prototype show that unmodified single-process applications execute 2 to 3 times faster and multiple concurrent processes execute 6 to 7.7 times faster, when compared to disk based paging.