Abstract: Embodiments focus on imparting a horizontally shifting motion to a plant punch element 1 during a part of a plant punch cycle 18 so as to reduce contact of a plant punching surface 38 with the plant during the plant punch. Plant punch elements that are particularly suited for such “shifting” capability may include a gap 41 facilitating lateral movement of the plant punch element to a position around at least part of the plant to be punched. Aspects of the inventive technology also address a sheltering plant punch apparatus that can also reduce injury and stress experienced by the plant while it is punched. Combinations of aspects disclosed herein provide a high degree of protection to the plant during a plant punch, thereby reducing stress experienced by the plant during the punch.

Abstract: Tissue culture medium such as porous frameworks and even open surface multidirectional porous frameworks may be used to provide uniform distribution of nourishment solutions, uniform interstitial voids as well as undistorted transport fields which may facilitate high volume yields of finished plants from cells, such as explants in a tissue culturing process. Further embodiments may include automating a tissue culturing process to reduce labor costs and increase uniformity of finished plants through tissue culture processes.

Abstract: Embodiments focus on imparting a horizontally shifting motion to a plant punch element 1 during a part of a plant punch cycle 18 so as to reduce contact of a plant punching surface 38 with the plant during the plant punch. Plant punch elements that are particularly suited for such “shifting” capability may include a gap 41 facilitating lateral movement of the plant punch element to a position around at least part of the plant to be punched. Aspects of the inventive technology also address a sheltering plant punch apparatus that can also reduce injury and stress experienced by the plant while it is punched. Combinations of aspects disclosed herein provide a high degree of protection to the plant during a plant punch, thereby reducing stress experienced by the plant during the punch.

Abstract: A growing system for plant seedlings that takes into consideration the varied requirements of the seedling; seedling and transplant environment; seedling cell size; mechanical transplanter requirements; seedling leaf, stem and root development and coordinates all these requirements to increase transplanting yields which may include larger containers that maximize yield and minimize damage. Aspects may eliminate left-over seedlings, reduce the number of manual steps, decrease labor, and increase transplant yields.

Abstract: A transplanting system including both methods and specific types of transplant apparatus such as propagule transplant trays(3, 4, 5, 6) addresses the need for the use of a propagule punch machine (8) on a variety of adolescent tray configurations (9, 11, 12, e.g.) and the increased operational efficiency that such enhanced operational capability affords. The system may involve at least one areally continuous propagule tray (3, 4, 5, 6) that is operationally compatible with a numerically significant number of adolescent plant trays. Other embodiments of the invention disclose punch pins (59) for use in a plant punch machine transplanter that are user-detachable and differentially designed so that plant punch elements of a certain design may be installed as part of the machine to customize the transplant process for a given species and transplant modality. The invention also provides for a consultable collection (such as printed documents or an electronic database, e.g.

Abstract: A growing system for plant seedlings that takes into consideration the varied requirements of the seedling; seedling and transplant environment; seedling cell (312) size; mechanical transplanter requirements; seedling leaf, stem and root development and coordinates all these requirements to increase transplanting yields may include larger containers that maximize yield and minimize damage. Aspects may eliminate left-over seedlings, reduce the number of manual steps, decrease labor, and increase transplant yields.

Abstract: A growing system for plant seedlings that takes into consideration the varied requirements of the seedling; seedling and transplant environment; seedling cell (312) size; mechanical transplanter requirements; seedling leaf, stem and root development and coordinates all these requirements to increase transplanting yields may include larger containers that maximize yield and minimize damage. Aspects may eliminate left-over seedlings, reduce the number of manual steps, decrease labor, and increase transplant yields.

Abstract: A transplanting system including both methods and specific types of transplant apparatus such as propagule transplant trays(3, 4, 5, 6) addresses the need for the use of a propagule punch machine (8) on a variety of adolescent tray configurations (9, 11, 12, e.g.) and the increased operational efficiency that such enhanced operational capability affords. The system may involve at least one areally continuous propagule tray (3, 4, 5, 6) that is operationally compatible with a numerically significant number of adolescent plant trays. Other embodiments of the invention disclose punch pins (59) for use in a plant punch machine transplanter that are user-detachable and differentially designed so that plant punch elements of a certain design may be installed as part of the machine to customize the transplant process for a given species and transplant modality. The invention also provides for a consultable collection (such as printed documents or an electronic database, e.g.

Abstract: A gene which encodes an ACC synthase is isolated from rose plants, specifically Rosa (cardinal red). This gene is modified for expression in transgenic plants. Isolation of high quality mRNA for gene isolation is achieved through use and adaptation of a 2-butoxyethanol precipitation technique using large amount of initial tissue in order to achieve critical mass for precipitation.