Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.

Abstract: A folded Sagnac fiber optic acoustic sensor array operates in a manner similar to a Sagnac interferometer but uses a common delay path to reduce distributed pickup in downlead fibers. The fiber optic acoustic sensor array is used to detect acoustic waves in water. By basing the folded Sagnac sensor array on operating principles similar to the Sagnac interferometer rather than basing the array on a Mach-Zehnder interferometer, the sensor array has a stable bias point, has reduced phase noise, and allows a broadband signal source to be used rather than requiring a more expensive narrowline laser. A large number of acoustic sensors can be multiplexed into the architecture of the folded Sagnac fiber optic acoustic array.