Abstract: Embodiments concern methods and composition related to anergic T-cells in patients, such as cancer patients.

Abstract: Described herein is a method for evaluating a subject comprising detecting genetic mutation(s) in the DNA sequence of one or more oral cavity squamous cell carcinoma (OCSCC) biomarker(s) in a biological sample from the subject comprising DNA, wherein the OCSCC biomarker(s) comprise TP53, CDKN2A, FAT1, CASP8, NOTCH1, PIK3CA, and/or ERAS.

Abstract: The present invention concerns methods and compositions for treating or preventing a bacterial infection, particularly infection by a Staphylococcus bacterium. The invention provides methods and compositions for stimulating an immune response against the bacteria. In certain embodiments, the methods and compositions involve a non-toxigenic Protein A (SpA) variant.

Abstract: The methods and compositions described herein address the need in the art by providing peptides and polypeptides comprising a growth factor binding domain. In some embodiments, the peptides have an amino acid sequence that is at least 80% identical to one of SEQ ID NOS:1-7, 13-15, 49-50, or 66-70, or a fragment thereof; wherein the peptide is less than 300 amino acids in length.

Abstract: Methods and systems for computationally processing data with a multi-layer convolutional neural network (CNN) having an input and output layer, and one or more intermediate layers are described. Input data represented in a form of evaluations of continuous functions on a sphere may be received at a computing device and input to the input layer. The input layer may compute outputs as covariant Fourier space activations by transforming the continuous functions into spherical harmonic expansions. The output activations from the input layer may be processed sequentially through each of the intermediate layers. Each, intermediate layer may apply Ciebsch-Gordan transforms to compute respective covariant Fourier space activations as input to an immediately next layer, without computing any intermediate inverse Fourier transforms or forward Fourier transforms.

Abstract: Technologies for tuning a resistance of tunnel junctions such as Josephson junctions are disclosed. In the illustrative embodiment, a Josephson junction is heated to 85 Celsius, and an electric field is applied to the Josephson junction. The heat and the electric field cause the resistance of the Josephson junction to increase. Monitoring the Josephson junction during the application of the electric field allows for the resistance of the Josephson junction to be adjusted to a particular value.

Abstract: Stable, macroscopic single-crystal chiral liquid crystal compositions are described. The compositions include a single-crystal chiral liquid crystal material on a patterned surface. The patterned surface seeds a particular crystallographic orientation at the substrate-liquid crystal interface. Also described are methods of forming the single-crystal chiral liquid crystal compositions.

Abstract: Microfluidic devices, systems, and methods.

Abstract: A proximity dependent split T7 RNAP (RNA polymerase) sensor using continuous molecular evolution is described. The versatility of the platform is described by creating robust light and small molecule-responsive genetic sensors. The activity-responsive RNAP platform dramatically simplifies and expands genetic circuit creation, and opens new opportunities in protein engineering, synthetic biology, and bioengineering.

Abstract: Aspects of the present disclosure relate to polypeptides that specifically bind to a SARS-CoV-2 spike protein, and to methods of use for treatment, prevention, and diagnosis of a SARS-CoV-2 infection. Certain aspects are directed to antibodies and fragments thereof, including Fabs, configured to bind to various epitopes within the receptor binding domain (RBD) of a SARS-CoV-2 spike protein. Compositions, kits, and methods for detection of SARS-CoV-2 are also disclosed.

Abstract: The methods, compositions, and kits of the disclosure provide a novel approach for a whole genome, unbiased DNA analysis method that can be performed on limited amounts of DNA. can be used to analyze DNA to determine its modification status. Aspects of the disclosure relate to a method for amplifying bisulfite-treated deoxyribonucleic acid (DNA) molecules comprising: (a) ligating an adaptor to the DNA molecules, wherein the adaptor comprises a RNA polymerase promoter comprising bisulfite-protected cytosines; (b) treating the ligated DNA molecules with bisulfite; (c) hybridizing the bisulfite-treated DNA molecules with a primer; (d) extending the hybridized primer to make double stranded DNA; and (e) in vitro transcribing the double-stranded DNA to make RNA.

Abstract: Aspects of the present disclosure provide methods and compositions for treatment of autoimmune disorders including, for example, multiple sclerosis and rheumatoid arthritis. Also disclosed are methods for promoting wound healing. Certain aspects are directed to antiinflammatory cytokines operatively linked to an albumin protein. Further aspects relate to methods for delivering an anti-inflammatory cytokine to a lymph node.

Abstract: Polymeric membranes are modified via SIS to promote membrane resilience, prolong membrane lifetime, and mitigate fouling. Modified membranes include an inorganic material within an outer portion of the modified membrane and a polymeric core that remains unmodified by the inorganic material. The polymer may be removed leaving an inorganic material patterned from an initial unmodified polymeric membrane.

Abstract: Described herein are nucleic acid molecules and complexes useful as i-switch pH reporters that have increased sensitivities as a pH reporter and have alternate pH reporting capacity ranges. Aspects of the disclosure relate to a method for determining pH comprising providing a nucleic acid complex comprising: a first single-stranded nucleic acid molecule comprising the sequence CnXCnYCnZCn (SEQ ID NO.

Abstract: The engineered polypeptide comprising modified Fab constant regions and/or protein G Fab binding domains provide advanced affinity reagents that can be used in cell biology applications as well as for therapeutic applications. Accordingly, aspects of the disclosure relate to a polypeptide comprising a constant region of an antibody light chain, wherein the constant region comprises a substitution/deletion of amino acids corresponding to positions 16-20 of SEQ ID NO:1 of the constant region with the amino acids LRT.

Abstract: Aspects of the disclosure are directed to methods, systems, and compositions for detecting antibodies capable of binding to angiotensin II. Certain aspects comprise detection of antibodies capable of binding to angiotensin II in a sample from a subject, where the subject has or has had a coronavirus infection, such as a SARS-CoV-2 infection. Also disclosed are vaccine compositions comprising a portion of SARS-CoV-2 Spike protein, where such compositions do not induce generation of angiotensin II-binding antibodies.

Abstract: Methods and compositions for treating cancer are disclosed.

Abstract: Embodiments of the invention are directed to methods of determining the prognosis of a breast cancer patient by evaluating the activity of the glucocorticoid receptor in tumor cells. Other embodiment include methods of treating breast cancer cells, particularly, chemo-resistant cells, with a glucocorticoid receptor antagonist and an anticancer agent or compound.

Abstract: A computing system includes a quantum processor with qubits, a classical memory including a quantum program defining a plurality of instructions in a source language, and a classical processor configured to: (i) receive a circuit of gates representing a quantum program for a variational algorithm in which computation is interleaved with compilation; (ii) identify a plurality of blocks, each block includes a subcircuit of gates, leaving one or more remainder subcircuits of the circuit of gates outside of the plurality of blocks; (iii) pre-compile each block of the plurality of blocks with a pulse generation program to generate a plurality of pre-compiled blocks including control pulses configured to perform the associated block on the quantum processor; and (iv) iteratively execute the quantum program using the pre-compiled blocks as static during runtime and recompiling the one or more remainder subcircuits on the classical processor at each iteration of execution.

Abstract: Provided herein are compositions and methods for the inhibition of enteric infection. In particular, compositions comprising bacteria of the class Clostridia are administered to human and/or animal subjects to prevent or decrease susceptibility to enteric infection.