Patents Assigned to Therapeutic Proteins International, LLC
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Patent number: 9238789Abstract: A flexible disposable bioreactor having three, stagger-baffled compartments wherein the middle compartment houses a sparging rod is described to provide the highest degree of sparging and mixing to produce biological products.Type: GrantFiled: July 21, 2012Date of Patent: January 19, 2016Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Patent number: 9200335Abstract: A bioreactor that combines the steps of recombinant expression and separation of a biological product by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction. The method also allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor.Type: GrantFiled: February 21, 2014Date of Patent: December 1, 2015Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Publication number: 20150290597Abstract: An aeration and mixing device for disposable flexible bioreactors comprising a mesh of interconnected perforated disposable tubes to form a structure to cover essentially the entire bottom surface of a disposable flexible bioreactor and wherein a continuous flow of gases through the perforations in the tubes provides an aeration and a mixing function.Type: ApplicationFiled: April 9, 2014Publication date: October 15, 2015Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Publication number: 20150275318Abstract: A method of homogenously mixing the contents of a plurality of bioreactors by providing a receiving container capable of holding an appropriate quantity of the liquid and repeatedly raising and lowering the receiving container to a position above or below the position of the bioreactors resulting in mixing the contents of the bioreactors.Type: ApplicationFiled: June 12, 2015Publication date: October 1, 2015Applicant: THERAPEUTIC PROTEINS INTERNATIONAL, LLCInventor: Sarfaraz K. Niazi
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Publication number: 20150253022Abstract: A single-pass HVAC systems to isolate zones and to maintain a required clean air quality standard is provided that operates by producing a positive pressure in all zones, while exhausting on a minimal quantity of air required by law. The zones are kept clean by a recirculating fan filter in each zone. The exhaust air is used to exchange heat with incoming air to conserve energy further.Type: ApplicationFiled: May 20, 2015Publication date: September 10, 2015Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Patent number: 9101857Abstract: Fine gas bubbles traveling at fast speed are employed to scrub a hard-surface filter for harvesting liquids from suspensions including those consisting of nutrient media and cell culture, on a continuous basis without clogging the filters.Type: GrantFiled: April 26, 2011Date of Patent: August 11, 2015Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz Niazi
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Publication number: 20150210974Abstract: An exhaust system suitable for high volume exhaust from flexible disposable bags is described that prevents nutrient media volume loss and prevents cross-contamination without using any filters. The invention described here allows the use of disposable two-dimensional bioreactors for the cultivation of bacterial and other organisms and cells require high aeration.Type: ApplicationFiled: April 13, 2015Publication date: July 30, 2015Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. NIAZI
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Patent number: 9068215Abstract: A method of homogenously mixing the contents of a plurality of bioreactors by providing a receiving container capable of holding an appropriate quantity of the liquid and repeatedly raising and lowering the receiving container to a position above or below the position of the bioreactors resulting in mixing the contents of the bioreactors.Type: GrantFiled: May 13, 2011Date of Patent: June 30, 2015Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Patent number: 9005959Abstract: An exhaust system suitable for high volume exhaust from flexible disposable bags is described that prevents nutrient media volume loss and prevents cross-contamination without using any filters. The invention described here allows the use of disposable two-dimensional bioreactors for the cultivation of bacterial and other organisms and cells require high aeration.Type: GrantFiled: May 1, 2011Date of Patent: April 14, 2015Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz Niazi
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Publication number: 20150099293Abstract: A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification.Type: ApplicationFiled: December 12, 2014Publication date: April 9, 2015Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Patent number: 8932843Abstract: A buoyant device containing chromatography media performs the function of protein harvesting replacing the steps of cell separation and volume reduction; the device can be loaded into columns for further purification.Type: GrantFiled: September 26, 2011Date of Patent: January 13, 2015Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz Niazi
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Publication number: 20140356968Abstract: The present disclosure provides methods to assess structural similarity of a first biomolecule and a second biomolecule by detecting one or more responses of the first and second biomolecule to thermodynamic stress conditions induced by osmotic and dielectric changes including, detecting a shift in fluorescence emission and/or a change in the intensity of the emission.Type: ApplicationFiled: August 7, 2013Publication date: December 4, 2014Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Patent number: 8852435Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.Type: GrantFiled: November 29, 2011Date of Patent: October 7, 2014Assignee: Therapeutics Proteins International, LLCInventor: Sarfaraz Niazi
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Publication number: 20140175029Abstract: A method and an apparatus for separating suspended matter from liquid includes a concentrator filter that draws the liquid out of the suspension while the filter kept unblocked by a sparging filter that allows scrubbing of the concentrator filter by gas bubbles. This invention can be used to replace cross-flow filtration and centrifugation in the bioprocess industry and to reduce the volume of suspensions to concentrate the yield of the end product in the chemical industry.Type: ApplicationFiled: February 27, 2014Publication date: June 26, 2014Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Publication number: 20140170635Abstract: A bioreactor that combines the steps of recombinant expression and separation of a biological product by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction. The method also allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor.Type: ApplicationFiled: February 21, 2014Publication date: June 19, 2014Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Patent number: 8685245Abstract: A method and an apparatus for separating suspended matter from liquid includes a concentrator filter that draws the liquid out of the suspension while the filter kept unblocked by a sparging filter that allows scrubbing of the concentrator filter by gas bubbles. This invention can be used to replace cross-flow filtration and centrifugation in the bioprocess industry and to reduce the volume of suspensions to concentrate the yield of the end product in the chemical industry.Type: GrantFiled: May 31, 2011Date of Patent: April 1, 2014Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz Niazi
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Patent number: 8668886Abstract: A bioreactor that combines the steps of recombinant expression and separation of a biological product by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction. The method also allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor.Type: GrantFiled: April 24, 2011Date of Patent: March 11, 2014Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz Niazi
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Patent number: 8663474Abstract: A non-blocking filtration system wherein the suspension filtered continuously scrubs the filter, keeping it free of deposits of solid deposits while the filtrate is removed in a variety of biological and chemical applications, substantially reducing the cost of unit operations.Type: GrantFiled: April 3, 2013Date of Patent: March 4, 2014Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Publication number: 20130344584Abstract: A pair or receptacles capable of housing an emitter probe and a detector probe installed inside a bioreactor to monitor the properties of the nutrient media without contacting the nutrient media.Type: ApplicationFiled: August 28, 2013Publication date: December 26, 2013Applicant: Therapeutic Proteins International, LLCInventor: Sarfaraz K. Niazi
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Patent number: 8545759Abstract: A pair or receptacles capable of housing an emitter probe and a detector probe are installed inside a bioreactor to monitor the properties of the nutrient media without contacting the nutrient media.Type: GrantFiled: October 21, 2011Date of Patent: October 1, 2013Assignee: Therapeutic Proteins International, LLCInventor: Sarfaraz Niazi