Patents Assigned to Tokyo Women's Medical University
  • Publication number: 20200368972
    Abstract: The present invention provides a tubular tissue preparation device provided with: a chamber for accommodating a tubular support body; a first support part for communicating the inside and the outside of the chamber and fixing the tubular support body; and a pressurizing/depressurizing means for controlling the pressure in an inner space of the chamber, wherein the first support part is for fixing a first opening section of the tubular support body, communicating a lumen of the tubular support body and the outside of the chamber, and separating the lumen of the tubular support body and the inner space of the chamber. Also, the present invention provides a tubular tissue preparation kit that includes said device and a device for transporting sheet-shaped tissue. In addition, the present invention provides a tubular tissue preparation method using said device.
    Type: Application
    Filed: October 19, 2018
    Publication date: November 26, 2020
    Applicant: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Shogo SHIMIZU, Kazunori SANO, Hidekazu SEKINE, Tatsuya SHIMIZU
  • Publication number: 20200352550
    Abstract: The present invention relates to a therapeutic substance delivery device for delivering a therapeutic substance to a desired site in a bodily duct, characterized in that the therapeutic substance delivery device is provided with: a therapeutic substance loading portion; a connector which is connected to the therapeutic substance loading portion; and a supplying/discharging pipe connected to the connector; and in that the therapeutic substance loading portion includes a main body portion in which a recessed portion is formed, a resilient film, and a connecting pipe; the connector is provided with a joint main body, a flange portion fixed to the other end portion side of the joint main body, and a fixing nut through which the joint main body passes; the joint main body is provided with a tube fastening portion; and at least part of the inner wall of the fixing nut is provided with a thread.
    Type: Application
    Filed: September 8, 2016
    Publication date: November 12, 2020
    Applicant: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Masanori Maeda, Nobuo Kanai, Yusuke Fujii, Masayuki Yamato, Makoto Abe
  • Publication number: 20200291350
    Abstract: The presently disclosed subject matter is to provide a hepatocyte structure, particularly, a hepatocyte structure that mimics nonalcoholic steatohepatitis (NASH). A hepatocyte construct including an aggregate containing hepatocytes and adherent cells that are non-hepatocytes, and wherein the hepatocytes include ballooned hepatocytes is provided. Further, a method for producing a hepatocyte construct, comprising: (i) forming an aggregate by aggregating a cell group comprising hepatocytes and adherent cells that are non-hepatocytes; and (ii) culturing the aggregate is provided.
    Type: Application
    Filed: July 3, 2019
    Publication date: September 17, 2020
    Applicants: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Botao GAO, Yuki KAGAWA, Tetsuya OGAWA, Hirotsugu KUBO, Tatsuya SHIMIZU, Katsuhisa SAKAGUCHI
  • Patent number: 10717961
    Abstract: A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.
    Type: Grant
    Filed: April 24, 2013
    Date of Patent: July 21, 2020
    Assignees: ASAHI KASEI KABUSHIKI KAISHA, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Ushio Iwamoto, Michi Sato, Kanako Konishi, Katsuhisa Matsuura, Tatsuya Shimizu, Teruo Okano
  • Patent number: 10711247
    Abstract: The present invention pertains to a method for culturing a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells at a temperature of 40.5° C. or higher and reducing the pluripotent stem cells included in the cell population. The present invention also pertains to a method for reducing pluripotent stem cells from a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells, wherein the method includes a step for activating the TRPV-1 expressed in the pluripotent stem cells included in the cell population. The present invention makes it possible to reduce the pluripotent stem cells remaining in an undifferentiated state when inducing the differentiation of a pluripotent stem cell population.
    Type: Grant
    Filed: August 23, 2016
    Date of Patent: July 14, 2020
    Assignee: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Katsuhisa Matsuura, Tatsuya Shimizu, Hiroyoshi Seta
  • Patent number: 10688133
    Abstract: The present invention provides a method of inhibiting progression of or preventing renal disorder, the method comprising applying a cell sheet composition for inhibiting progression of or preventing renal disorder to at least one part of the surface of a kidney, wherein the part of the surface is uncovered by a fibrous capsule of the kidney. The present invention also provides a cell sheet composition for inhibiting progression of or preventing renal disorder, the cell sheet composition comprising a cell that has a function of producing a hepatocyte growth factor (HGF). The present invention also provides a method of producing a cell sheet composition for inhibiting progression of or preventing renal disorder.
    Type: Grant
    Filed: January 25, 2017
    Date of Patent: June 23, 2020
    Assignee: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Masatoshi Oka, Sachiko Sekiya, Tatsuya Shimizu, Kosaku Nitta, Teruo Okano
  • Publication number: 20200140807
    Abstract: The present invention provides a method for swiftly producing a layered cell sheet that is non-invasively obtained and is utilizable for transplantation, etc., the method including (1) a step of applying a centrifugal force to a first cell sheet on a temperature-responsive culture surface for a predetermined time in a temperature range from a lower critical solution temperature of the temperature-responsive culture surface to 45° C., (2) a step of further placing a second cell sheet on the first cell sheet, and (3) a step of applying a centrifugal force to the first cell sheet and the second cell sheet on the temperature-responsive culture surface for a predetermined time in the temperature range from the lower critical solution temperature to 45° C.; and also provides a layered cell sheet obtained by the method.
    Type: Application
    Filed: June 28, 2018
    Publication date: May 7, 2020
    Applicants: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Yuki KAGAWA, Tatsuya SHIMIZU, Yuji HARAGUCHI, Hirotsugu KUBO
  • Publication number: 20200030409
    Abstract: Provided is a LYPD1 inhibitor for promoting vascular endothelial network formation in a biological tissue. Also provided is a medicinal composition, said medicinal composition comprising a LYPD1 inhibitor as an active ingredient, for treating and/or preventing neoangiogenic disorders. Also provided is a method which comprises: (a1) a step for providing a cell population containing first cells expressing LYPD1 and vascular endothelial cells; (a2) a step for treating the cell population obtained in step (a1) with a LYPD1 inhibitor; and (a3) a step for culturing the cell population obtained in step (a2).
    Type: Application
    Filed: March 6, 2018
    Publication date: January 30, 2020
    Applicant: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Katsuhisa Matsuura, Tatsuya Shimizu, Shinako Aoki, Satoru Sakamoto
  • Patent number: 10368980
    Abstract: The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: August 6, 2019
    Assignees: Tokyo Women's Medical University, The Jikei University, CellSeed Inc.
    Inventors: Takanori Hama, Hiromi Kojima, Hiroshi Moriyama, Masayuki Yamato, Teruo Okano
  • Patent number: 10365264
    Abstract: A measuring apparatus relates to a contractile ability of a spontaneously pulsating myocardial tissue. The measuring apparatus includes a measurement table and a pressure sensor. The measurement table includes a storage section having an opening and configured to hold liquid inside the storage section, and an attaching portion on which the myocardial tissue is attached around the opening. The pressure sensor is disposed inside the storage section.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: July 30, 2019
    Assignees: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Hirotsugu Kubo, Tatsuya Shimizu, Tetsutaro Kikuchi
  • Publication number: 20190120742
    Abstract: A device for measuring a tension of a sheet-like tissue containing cardiomyocytes includes a first gel adapter holder having a frame member and a first gel holding member protruding toward a part of an inside face of the frame member for fixing one end of a film-like gel; and a second gel adapter holder having a second gel holding member for fixing the other end of the gel and a connection member connected to the second gel holding member. A kit includes the tension measuring device; a substrate having a pair of gel molding protruding members fitted along the inside face of the frame member; and a gel forming lid body having a face parallel to a gel contact face of the substrate so as to form an upper face of the gel. Further, a system for measuring the tension includes the tension measuring device.
    Type: Application
    Filed: October 24, 2018
    Publication date: April 25, 2019
    Applicants: NIHON KOHDEN CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Hirotsugu KUBO, Takahiro SHIOYAMA, Yuki KAGAWA, Tatsuya SHIMIZU, Daisuke SASAKI
  • Patent number: 10251916
    Abstract: An object of the present invention is to provide a cell composition for treatment of uterine tissue that heals damage occurring in uterine tissue, including intrauterine adhesion, to a state that enables pregnancy. In addition, an object of the present invention is to provide a method for producing this cell composition for treatment of uterine tissue, and a cell composition for treatment of uterine tissue produced according to that method. The cell composition, which has a first cell layer containing epithelial cells and a second cell layer containing stromal cells, and the first cell layer is laminated on the second cell layer, has the ability to heal damage occurring in uterine tissue, including intrauterine adhesion, and restore it to a state that enables pregnancy.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: April 9, 2019
    Assignee: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Goro Kuramoto, Soichi Takagi, Tatsuya Shimizu, Teruo Okano
  • Publication number: 20190054123
    Abstract: The present invention addresses the problem of providing a cell sheet composition for healing or preventing discharge from a wounded region of a luminal organ. The present invention also addresses the problem of providing a method in which the cell sheet composition is affixed to a wounded region of a luminal organ to heal or prevent discharge from the wounded region of the luminal organ. The present invention provides a cell sheet composition including mesenchymal stem cells which is characterized by being affixed to a wounded region of a luminal organ in order to heal or prevent discharge from the wounded region of the luminal organ. The present invention also provides a method in which the cell sheet composition including mesenchymal stem cells is affixed to a wounded region of a luminal organ to heal or prevent discharge from the wounded region of the luminal organ.
    Type: Application
    Filed: January 17, 2017
    Publication date: February 21, 2019
    Applicant: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Nobuo Kanai, Yasuhiro Maruya, Kurodo Koshino, Masanori Maeda, Teruo Okano
  • Patent number: 10094816
    Abstract: Provided is a means for evaluating the wetting characteristic of an object such as a cell sheet and a culture dish in a non-contact fashion. The wetting characteristic of an object is evaluated by a method comprising the steps of: (1) removing a liquid by jetting a gas at a surface of the object covered with the liquid, (2) measuring a dimension of a region in which the liquid is removed after the completion of the gas jetting and (3) evaluating the wetting characteristic of the object using the measured dimension as an index.
    Type: Grant
    Filed: May 24, 2013
    Date of Patent: October 9, 2018
    Assignee: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Nobuyuki Tanaka, Ryohei Uchida, Makoto Kondo, Masayuki Yamato, Teruo Okano, Makoto Kaneko
  • Patent number: 10064684
    Abstract: In an operation-mode indicating system, a first light emitting device is located within a peripheral visual field of an operator and is controllably connected to a controller. The peripheral visual field is defined around a center visual field of the operator while the operator is looking at a predetermined site. A body support apparatus is capable of performing an operation to the predetermined site. The controller controls the first light emitting device to emit first light in a first operation mode of the body support apparatus and second light in a second operation mode of the body support apparatus. The first light and the second light are visually distinguishable from each other.
    Type: Grant
    Filed: April 10, 2014
    Date of Patent: September 4, 2018
    Assignees: DENSO CORPORATION, SHINSHU UNIVERSITY, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Satoru Nakamura, Minoru Takahashi, Kazuhiro Hongo, Tetsuya Goto, Yosuke Hara, Jun Okamoto
  • Publication number: 20180245047
    Abstract: The present invention pertains to a method for culturing a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells at a temperature of 40.5° C. or higher and reducing the pluripotent stem cells included in the cell population. The present invention also pertains to a method for reducing pluripotent stem cells from a cell population including pluripotent stem cells and differentiated cells derived from pluripotent stem cells, wherein the method includes a step for activating the TRPV-1 expressed in the pluripotent stem cells included in the cell population. The present invention makes it possible to reduce the pluripotent stem cells remaining in an undifferentiated state when inducing the differentiation of a pluripotent stem cell population.
    Type: Application
    Filed: August 23, 2016
    Publication date: August 30, 2018
    Applicant: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Katsuhisa Matsuura, Tatsuya Shimizu, Hiroyoshi Seta
  • Publication number: 20180155673
    Abstract: An object of the present invention is to obtain a thicker animal cell composition by a simple and less expensive method. Namely, an object of the present invention is to provide a method for culturing a thicker animal cell composition by eliminating the hypoxia associated with animal cell compositions, a method for producing an animal cell composition containing unicellular algae, and an animal cell composition. The present invention provides a method for culturing an animal cell composition in a culture medium in the presence of unicellular algae and under exposure to light. According to the method of the present invention, oxygen can be continuously supplied in the culture medium, cell damage is alleviated, and a thicker cell composition can be obtained in the absence of a capillary network.
    Type: Application
    Filed: June 24, 2016
    Publication date: June 7, 2018
    Applicant: Tokyo Women's Medical University
    Inventors: Tatsuya Shimizu, Yuji Haraguchi, Yuki Kagawa, Katsuhisa Sakaguchi
  • Publication number: 20180149597
    Abstract: Provided is a determination device including a determining section that determines a state of a cell, using information relating to uniformity of a detection target generated based on an optical intensity of radiation light from the detection target included in a biological cell irradiated with excitation light. In the determination device, the detection target may be proteins. The determination device may include an information generating section that generated information, and the information generating section may generate information that excludes information corresponding to a non-resonant background signal.
    Type: Application
    Filed: December 21, 2017
    Publication date: May 31, 2018
    Applicants: NIKON CORPORATION, TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Tadashi Umezaki, Naoki Fukutake, Shunji Watanabe, Yusuke Taki, Yuji Haraguchi, Tatsuya Shimizu
  • Patent number: 9950094
    Abstract: Provided are layered cell sheets, comprising a plurality of layered cell sheets containing myoblasts, in which each cell sheet comprises cell population containing myoblasts with controlled orientations. Preferably provided are the layered cell sheets comprising a region in which the orientations of the cell population containing the myoblasts in each cell sheet are identical to each other.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: April 24, 2018
    Assignee: Tokyo Women's Medical University
    Inventors: Hironobu Takahashi, Tatsuya Shimizu, Teruo Okano
  • Patent number: 9919139
    Abstract: With the present invention the sheet shaped therapeutic substance can be accurately and easily affixed to the inside of the human body. As shown in FIG. 6A, the sheet shaped substance can be mounted on the head with wrinkles being formed. When this takes place, the sheet shaped substance is held in contact with the outer periphery of the head. Then, by making the pressure of the fluid inside the vent apertures positive, the sheet shaped substance can be blown off. The surface of the head is closely parallel to an inner surface of the esophagus wall for the sheet shaped substance to be affixed. Therefore, this particularly makes it possible to perform such applying step (FIG. 6B) in a more reliable fashion.
    Type: Grant
    Filed: October 23, 2013
    Date of Patent: March 20, 2018
    Assignee: Tokyo Women's Medical University
    Inventors: Nobuyuki Tanaka, Nobuo Kanai, Masayuki Yamato, Teruo Okano