Patents Assigned to University of Washington
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Publication number: 20240047098Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.Type: ApplicationFiled: October 11, 2023Publication date: February 8, 2024Applicant: University of WashingtonInventors: Daniel T. CHIU, Jiangbo YU, Yu RONG, Changfeng WU
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Publication number: 20240042101Abstract: Materials, methods, and systems for biorthogonal ligation of hydrogel labels to crosslinked-natural polymer hydrogels are provided. A heterobifunctional linker includes a peptide-reactive activated functional group on the heterobifunctional linker, including an activated amine-reactive functional group, an activated thiol-reactive functional group and being reactive with a hydrogel comprising a crosslinked natural polymer. The heterobifunctional linker also includes a photocaged reactive group including a photocaged hydroxylamine, a photocaged alkoxyamine, a photocaged hydrazide, a photocaged amine, a photocaged tetrazine, or a photocaged alkyne-containing moiety. The peptide-reactive activated functional group does not include an azide.Type: ApplicationFiled: December 20, 2021Publication date: February 8, 2024Applicant: University of WashingtonInventors: Cole DeForest, Ivan Batalov, Kelly Rose Stevens
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Publication number: 20240044048Abstract: Methods to create barcoded influenza viruses without disrupting the function of the viral proteins and the proper packaging of the viral genome segments are described. The barcoded influenza viruses can be used within deep mutational scanning libraries to map influenza resistance mutations to therapeutic treatments. The libraries can also be used to predict influenza strains that may become resistant to therapeutic treatments and/or more easily evolve to infect new species. The libraries include features that allow efficient collection and assessment of informative data, obviating bottlenecks of previous approaches.Type: ApplicationFiled: October 19, 2023Publication date: February 8, 2024Applicants: Fred Hutchinson Cancer Center, University of WashingtonInventors: Jesse Bloom, Allison Greaney, Andrea Loes, Adam S. Dingens
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Patent number: 11884649Abstract: Disclosed herein, inter alia, are compounds for inhibiting IRE1? and uses thereof.Type: GrantFiled: November 12, 2021Date of Patent: January 30, 2024Assignees: The Regents of the University of California, University of WashingtonInventors: Feroz R. Papa, Bradley J. Backes, Dustin J. Maly
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Patent number: 11883132Abstract: A system for the optical measurement of pH includes a light emitter to emit an excitation light, and a detector coupled to receive florescence light produced by a compound in a mouth of a patient in response to the excitation light. A controller is coupled to the detector, and the controller includes logic that when executed by the controller, causes the system to perform operations. The operations may include emitting the excitation light from the light emitter; measuring an intensity of the florescence light emitted from a surface of individual teeth in a plurality of teeth in the mouth; and determining, based on the intensity of the florescence light, one or more locations on the individual teeth likely to develop demineralization.Type: GrantFiled: October 27, 2017Date of Patent: January 30, 2024Assignee: University of WashingtonInventors: Eric J. Seibel, Yuanzheng Gong, Zheng Xu, Jeffrey S. McLean, Yaxuan Zhou
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Patent number: 11885946Abstract: Embodiments disclosed herein directed to multi-direction digital light sheet microscopy (mDSLM). In mDSLM a sample may be illuminated with an illumination sheet which has a first angular diversity along a first axis and a second angular diversity along a second axis which is orthogonal to the first axis. An mDSLM system includes beam shaping optics which may passively reshape light into the illumination sheet. In some embodiments, scanning optics may be used to scan the illumination sheet with respect to the sample. In some embodiments, a confocal aperture may be used to limit out of focus light which reaches a detector of the mDSLM system.Type: GrantFiled: January 25, 2019Date of Patent: January 30, 2024Assignee: University of WashingtonInventors: Adam K. Glaser, Jonathan T. C. Liu
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Publication number: 20240027325Abstract: Systems and methods for analyzing particles flowing in a channel are described. In an embodiment, the channel is configured to flow a particle through a lumen of the channel, the channel defining an interrogation window configured to allow light to pass into and out of the lumen; a light engine comprising: a first light source positioned to output first excitation light onto a first portion of the channel in the interrogation window; and a second light source positioned to output second excitation light onto a second portion of the channel in the interrogation window separate from the first portion. In an embodiment, the systems include an emission fiber bundle comprising a first emission optical fiber and a second emission optical fiber, wherein a proximal end of first emission optical fiber and second emission optical fiber are arranged in an emission fiber bundle head.Type: ApplicationFiled: November 10, 2021Publication date: January 25, 2024Applicant: University of WashingtonInventors: Daniel T. Chiu, Yifei Jiang, Mengxia Zhao, Yuanhua Cheng
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Publication number: 20240026443Abstract: The disclosure provides methods and related compositions and kits for mapping DNA-protein interactions (DPIs). In one aspect, the disclosed methods comprise contacting a double stranded DNA molecule with a target protein; coupling a double stranded DNA deaminase (DddA) to the target protein, before or after the contacting step; permitting deamination of one or more cytosine residues in a domain of the double stranded DNA molecule by the DddA to provide one or more uracil residues, wherein the domain comprises a site of interaction between the target protein and the double stranded DNA molecule; determining the sequence of at least a portion of the double stranded DNA molecule; and detecting the domain comprising one or more cytosine deamination events. The method can be controlled by use of DddA inhibitors. The method can also incorporate use of inhibiting a base-excision repair pathway when addressing DPIs in a cellular context.Type: ApplicationFiled: September 29, 2021Publication date: January 25, 2024Applicant: University of WashingtonInventors: Joseph D. Mougous, Marcos H. de Moraes, Snow Brook Peterson
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Publication number: 20240024826Abstract: Nanoporous selective sol-gel ceramic membranes, selective-membrane structures, and related methods are described. Representative ceramic selective membranes include ion-conductive membranes (e.g., proton-conducting membranes) and gas selective membranes. Representative uses for the membranes include incorporation into fuel cells and redox flow batteries (RFB) as ion-conducting membranes.Type: ApplicationFiled: April 7, 2023Publication date: January 25, 2024Applicant: University of WashingtonInventors: Gregory M. Newbloom, Aaron F. West, Ryan Kastilani, Canfeng Wei, Jaime Rodriguez, Lilo D. Pozzo, Lauren Martin
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Patent number: 11878499Abstract: In some embodiments, an apparatus, system, and method for activating a low-adhesion state of a thermal-sensitive tape is described. An example apparatus embodiment includes a light source and a temperature sensor. The light source is configured to illuminate a target area of the thermal-sensitive tape with a first spectrum of electromagnetic radiation to provide heating of the target area. The first spectrum including a first wavelength outside of a visible spectrum. The temperature sensor is configured to detect a second spectrum of electromagnetic radiation to approximate a temperature of the target area. The second spectrum includes a second wavelength different than the first wavelength.Type: GrantFiled: June 26, 2020Date of Patent: January 23, 2024Assignee: University of WashingtonInventors: Mark E. Fauver, Eric J. Seibel
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Publication number: 20240011055Abstract: Disclosed are methods and related compositions for genomic editing. In one aspect, methods of editing double stranded DNA (dsDNA) use first and second editing complexes specific for first and second target sequences on the sense and antisense strands of the dsDNA molecule, respectively. Each editing complex comprises an extended guide RNA associated with a fusion editor protein, which comprises a functional nickase domain and a functional reverse transcriptase domain. The respective guide RNAs guide their associated fusion editor proteins to the dsDNA, which implement single stranded breaks on opposite strands of the dsDNA. The respective reverse transcriptase domains generate 3? overhangs. Repair of the dsDNA excises the portion of dsDNA disposed between the two single-stranded breaks. A variety of configurations and applications of the method are disclosed, providing flexible, facile, efficient, and precise methods to impose genetic manipulations.Type: ApplicationFiled: November 4, 2021Publication date: January 11, 2024Applicant: University of WashingtonInventors: Jay Ashok Shendure, Wei Chen, Junhong Choi
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Patent number: 11866699Abstract: The application discloses multimeric assemblies packaging one or more active component and their use to carry out nucleic acid regulation or gene editing.Type: GrantFiled: February 12, 2018Date of Patent: January 9, 2024Assignee: UNIVERSITY OF WASHINGTONInventors: Marc Joseph Lajoie, Elizabeth Erin Gray, Neil P. King, Daniel Brewster Stetson, David Baker, Katherine DaPron
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Publication number: 20240005496Abstract: A method for determining a presence of arthritis in a patient, including obtaining a first image of a patient's joint, wherein the first image is a visible light image, obtaining a second image of the patient's joint, wherein the second image is a thermal light image, determining an outline of the patient's joint from the first image, determining an outline of a reference area from the first image, wherein the patient's joint is adjacent to the reference area, determining a first representative topological temperature within the outline of the patient's joint of the first image from the second image, determining a second representative topological temperature within the outline of the reference area of the first image from the second image, comparing the first representative topological temperature and the second representative topological temperature; and determining a likelihood of the presence of arthritis within the patient's joint.Type: ApplicationFiled: June 28, 2023Publication date: January 4, 2024Applicants: Seattle Children's Hospital dba Seattle Children's Research Institute, University of WashingtonInventors: Yongdong Zhao, Joshua Scheck, Savannah Corrina Partridge, Ramesh S. Iyer, Mahesh Thapa, Debosmita Biswas, Sr., Nivrutti Vasudev Bhide, Kevin Charles Cain, Jason Michael Pyke
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Publication number: 20240000295Abstract: Systems and methods for capturing and rendering light fields for head-mounted displays are disclosed. A mediated-reality visualization system includes a head-mounted display assembly comprising a frame configured to be mounted to a user's head and a display device coupled to the frame. An imaging assembly separate and spaced apart from the head-mounted display assembly is configured to capture light-field data. A computing device in communication with the imaging assembly and the display device is configured to receive light-field data from the imaging assembly and render one or more virtual cameras. Images from the one or more virtual cameras are presented to a user via the display device.Type: ApplicationFiled: March 23, 2023Publication date: January 4, 2024Applicant: University of WashingtonInventors: Joshua R. Smith, Samuel R. Browd, Rufus Griffin Nicoll, James Andrew Youngquist
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Publication number: 20240002907Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.Type: ApplicationFiled: August 24, 2023Publication date: January 4, 2024Applicant: University of WashingtonInventors: Georg Seelig, Richard Muscat, Alexander B. Rosenberg
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Patent number: 11857813Abstract: High intensity focused ultrasound systems for treating tissue are disclosed herein. A system of treating tissue in a patient in accordance with an embodiment of the present technology can include, for example, an ultrasound source having a focal region and configured to deliver high intensity focused ultrasound energy to a target site in tissue of the patient. The system can further include a controller operably coupled to the ultrasound source. The controller comprises a pulsing protocol for delivering the high intensity focused ultrasound energy with the ultrasound source to the target site. The controller is configured to cause the ultrasound source to pulse high intensity focused ultrasound waves to lyse cells in a volume of the tissue of the subject while preserving an extracellular matrix in the volume of the tissue exposed to the high intensity focused ultrasound waves.Type: GrantFiled: July 13, 2020Date of Patent: January 2, 2024Assignee: University of WashingtonInventors: Yak-Nam Wang, Michael R. Bailey, Tatiana D. Khokhlova, Wayne Kreider, Adam D. Maxwell, George R. Schade, Vera A. Khokhlova
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Publication number: 20230416247Abstract: Compounds and compositions for inhibiting miR-21, methods for inhibiting miR-21 in a subject, and methods for treating a disease or condition treatable by decreasing the level of microRNA miR-21 in a subject.Type: ApplicationFiled: September 28, 2021Publication date: December 28, 2023Applicant: University of WashingtonInventors: Matthew D. Shortridge, Gabriele Varani
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Publication number: 20230405145Abstract: A nanoparticle for targeted siRNA delivery comprising an iron oxide core and a chitosan-polyethylene glycol-polyethylenimine copolymer coating surrounding the core, chlorotoxin covalently coupled to the coating, and an siRNA reversibly associated to the coating by non-covalent interaction. Methods for making the nanoparticle and methods for using the nanoparticle to suppress the expression of O6-methylguanine-DNA methyltransferase (MGMT), treating brain cancer, and killing cancer stem cells.Type: ApplicationFiled: October 6, 2021Publication date: December 21, 2023Applicant: University of WashingtonInventors: Miqin Zhang, Richard G. Ellenbogen, Kui Wang, Forrest M. Kievit, John R. Silber, Zachary Stephen, Peter A. Chiarelli
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Publication number: 20230408526Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.Type: ApplicationFiled: November 23, 2022Publication date: December 21, 2023Applicant: University of Washington through its Center for CommercializationInventors: Daniel T. Chiu, Changfeng Wu, Xuanjun Zhang, Jiangbo Yu, Fangmao Ye
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Publication number: 20230407570Abstract: The present disclosure describes a method for preparing a paper nanocomposite, including: continuously providing a first suspension that includes lignocellulosic pulp fibers, cellulose nanofibrils, carbon nanotubes, and a cationic surfactant; continuously adding a second suspension to the first suspension to provide a slurry, the second suspension includes lignocellulosic pulp fibers, cellulose nanofibrils, carbon nanotubes, and an anionic surfactant; depositing the slurry comprising the first and second suspensions onto the substrate; and dewatering the slurry to form the paper nanocomposite.Type: ApplicationFiled: October 28, 2021Publication date: December 21, 2023Applicant: University of WashingtonInventors: Anthony B. Dichiara, Sheila B. Goodman