Abstract: A macromolecular water-soluble conjugates based on synthetic copolymers to which at least one affinity tag, at least one imaging probe and at least one targeting ligand are bound via covalent bonds. The macromolecular conjugate may be used in identification, visualization, quantification or isolation of proteins and/or cells.
Type:
Grant
Filed:
September 12, 2018
Date of Patent:
May 28, 2019
Assignees:
USTAV ORGANICKE CHEMIE A BIOCHEMIE AV CR, V.V.I., USTAV MAKROMOLEKULARNI CHEMIE AV CR, V.V.I., UNIVERZITA KARLOVA V PRAZE, PRIRODOVEDECKA FAKULTA
Inventors:
Pavel Sacha, Jan Konvalinka, Jiri Schimer, Tomas Knedlik, Vaclav Navratil, Jan Tykvart, Frantisek Sedlak, Pavel Majer, Petr Cigler, Vladimir Subr, Karel Ulbrich, Jiri Strohalm
Abstract: A macromolecular water-soluble conjugates based on synthetic copolymers to which at least one affinity tag, at least one imaging probe and at least one targeting ligand are bound via covalent bonds. The macromolecular conjugate may be used in identification, visualization, quantification or isolation of proteins and/or cells.
Type:
Application
Filed:
September 12, 2018
Publication date:
January 31, 2019
Applicants:
USTAV ORGANICKE CHEMIE A BIOCHEMIE AV CR, V.V.I., USTAV MAKROMOLEKULARNI CHEMIE AV CR, V.V.I., UNIVERZITA KARLOVA V PRAZE, PRIRODOVEDECKA FAKULTA
Inventors:
PAVEL SACHA, JAN KONVALINKA, JIRI SCHIMER, TOMAS KNEDLIK, VACLAV NAVRATIL, JAN TYKVART, FRANTISEK SEDLAK, PAVEL MAJER, PETR CIGLER, VLADIMIR SUBR, KAREL ULBRICH, JIRI STROHALM
Abstract: Macromolecular water-soluble conjugates based on synthetic copolymers to which at least one affinity tag, at least one imaging probe, and at least one targeting ligand are bound via covalent bonds. The macromolecular conjugate may be used in identification, visualization, quantification or isolation of proteins and/or cells. The targeting ligand may be attached to the synthetic copolymer via a flexible linker.
Type:
Application
Filed:
January 13, 2016
Publication date:
February 22, 2018
Applicants:
USTAV ORGANICKE CHEMIE A BIOCHEMIE AV CR, V.V.I., USTAV MAKROMOLEKULARNI CHEMIE AV CR, V.V.I., UNIVERZITA KARLOVA V PRAZE, PRIRODOVEDECKA FAKULTA
Inventors:
Pavel Sacha, Jan KONVALINKA, Jiri SCHIMER, Tomas KNEDLIK, Vaclav NAVRATIL, Jan TYKVART, Frantisek SEDLAK, Pavel MAJER, Petr CIGLER, Vladimir SUBR, Karel ULBRICH, Jiri STROHALM
Abstract: The invention relates to a method of preparation of a soluble formulation of water-insoluble pentacyclic and tetracyclic terpenoids, wherein the water-insoluble terpenoid having a free carboxylic, hydroxy or amino functional group is derivatized on this functional group with a substituent selected from the group comprising substituents of general formula Xa bound to the hydroxy group of the terpenoid, wherein Xa is —OC—R—COOH, substituents of general formula Xa bound to the amino group of the terpenoid, wherein Xa is —OC—R—COOH, quarternary ammonium substituents of general formula Xb bound to the carboxy group of the terpenoid, wherein Xb is —(CH2)nN+R3Y—, quarternary ammonium substituents of general formula Xc bound to the carboxy group of the terpenoid, wherein Xc je —(CH2)nR+Y—, substituents of general formula Xd bound to the carboxy group of the terpenoid, wherein Xd represents —R—COOH, glycosylic substituents Xe bound by alpha or beta glycosidic bond to the hydroxy group or to the carboxy group of the te
Type:
Grant
Filed:
September 25, 2007
Date of Patent:
February 18, 2014
Assignees:
Univerzita Karlova V Praze, Prirodovedecka Fakulta, Univerzita Palackeho V Olomouci, I.Q.A., A.S.
Inventors:
Jan Sarek, Marian Hajduch, Michal Svoboda, Katerina Novakova, Pavla Spacilova, Tomas Kubelka, David Biedermann
Abstract: The invention relates to a method of preparation and isolation of betulin diacetate from birch bark from paper mills and its optional processing to betulin, wherein the birch bark is ground, mixed with two- to twenty-fold excess volume of water and is stirred so that all birch bark is dipped, then the fraction of the birch bark which floats on the surface is collected, said collected fraction is dried at the temperature in the range of from 50 to 85° C. and the betulin contained in the dried birch bark is then subjected to an acetylation reaction, after the acetylation the birch bark is dried at the temperature of from 50 to 100° C. and the formed betulin diacetate is then isolated from the birch bark by supercritical extraction of the birch bark with carbon dioxide at the pressure of 28-35 MPa and the temperature of from 50 to 100° C. and subsequently, the supercritical extract is recrystallized for direct use or for further processing.
Type:
Grant
Filed:
January 9, 2008
Date of Patent:
January 10, 2012
Assignees:
Univerzita Karlova V Praze, Prirodovedecka Fakulta, Univerzita Palackeho V Olomouci and I.Q.A., a.s.
Inventors:
Jan Sarek, Michal Svoboda, Marian Hajduch
Abstract: The invention relates to a method of preparation of a soluble formulation of water-insoluble pentacyclic and tetracyclic terpenoids, wherein the water-insoluble terpenoid having a free carboxylic, hydroxy or amino functional group is derivatized on this functional group with a substituent selected from the group comprising substituents of general formula Xa bound to the hydroxy group of the terpenoid, wherein Xa is —OC—R—COOH, substituents of general formula Xa bound to the amino group of the terpenoid, wherein Xa is —OC—R—COOH, quarternary ammonium substituents of general formula Xb bound to the carboxy group of the terpenoid, wherein Xb is —(CH2)nN+R3Y—, quarternary ammonium substituents of general formula Xc bound to the carboxy group of the terpenoid, wherein Xc je —(CH2)nR+Y—, substituents of general formula Xd bound to the carboxy group of the terpenoid, wherein Xd represents —R—COOH, glycosylic substituents Xe bound by alpha or beta glycosidic bond to the hydroxy group or to the carboxy group of the te
Type:
Application
Filed:
September 25, 2007
Publication date:
December 31, 2009
Applicant:
UNIVERZITA KARLOVA V PRAZE PRIRODOVEDECKA FAKULTA
Inventors:
Jan Sarek, Marian Hajduch, Michal Svoboda, Katerina Novakova, Pavia Spacilova, Tomas Kubelka, David Biedermann