Abstract: The invention relates to an integrated tubular reaction device, which comprises a reaction vessel, a reaction vessel including at least two tubular chambers, a channel connecting at least two tubular chambers and an opening; a cover body, which can be worked with the opening, and a cover body including a through hole; a seal, which includes a sealing plug which can be worked with the through hole. The integrated tubular reaction device solves the problem of contamination of reaction products in the process of multiple or multi-step biological enzyme reaction, and can realize multiple or multi-step biological enzyme reactions in the same device.