Abstract: This invention provides a method for removal of all water soluble carbohydrates in the production of plant protein products, including oligosaccharides containing alpha-1,6-galactosidic bonds which are known to cause flatulence in man and animals. In the method an aqueous solution or dispersion of the plant protein material containing 1-60%, and preferably 15-50% of the dry matter is fermented with particular strains of Saccharomyces that have been found to degrade and assimilate flatus-causing carbohydrates. The fermentation is effected at a temperature between 20.degree. and 40.degree. C for from 12 to 48 hours, on which, if desired, the fermented material is dried.

Abstract: A method of producing soy sauce of superior quality by means of a large capacity closed-type tank in mass production and in a relatively short time, is characterized by the following steps in a soy sauce brewing method: charging soy sauce moromi into a closed large capacity tank provided with a hollow inner cylinder for circulation of moromi, adding enzyme to the system, on one hand feeding inert gas including the necessary minimum amount of oxygen, on the other hand degasing the fermentation system resulting in reduced pressure or vacuum at the top portion of the tank, removing carbon dioxide gas and thus promoting circulation and fermentation of moromi, and reutilizing the useful volatile constituent.

Abstract: There is disclosed a process for isomerization of glucose into fructose which comprises contacting an aqueous glucose solution with glucose isomerase in the presence of iron ion and magnesium ion, said glucose isomerase being in the form of insolubilized glucose isomerase adsorbed on a carrier.

Abstract: Thermostable lactase produced by growth of thermophilic fungi, harvesting resulting mycelium, separating lactase from mycelium by radical rupture of cell structure or by subjecting cell structure to milder treatment which does not separate lactase from the structure, and separating cell debris. Resulting lactase has high degree of activity and is thermostable such that it can be used at relatively high temperatures, e.g. 60.degree. C., and at acid pH to catalyze hydrolysis of lactose to glucose and galactose.

Abstract: This invention relates to a novel process for the preparation of the antibiotic Sulfomycin. The antibiotic is obtained by the fermentation of a nutrient medium with a Sulfomycin producing strain of the previously unknown microorganism Streptomyces cineroviridis.

Abstract: Readily soluble, sweetening compositions are produced by vacuum drum drying solutions of an edible bulking agent and a dipeptide sweetening compound.

Abstract: A method for producing L-cysteine and/or L-cystine which comprises:A. holding 2-amino-thiazoline-4-carboxylic acid in an aqueous solution at a pH of 5 to 11 in the presence of an effective amount of enzyme produced by a microorganism; said enzyme being capable of converting the 2-amino-thiazoline-4-carboxylic acid to L-cysteine and/or L-cystine, and said microorganism being capable of growing in a medium containing 2-amino-thiazoline-4-carboxylic acid as a nitrogen source; andB. recovering the L-cysteine and/or L-cystine formed from said aqueous solution.

Abstract: A method of determining lactate dehydrogenase activity in biological fluids comprising reacting a biological fluid sample with a coupled enzyme series in an oxygenated aqueous solution containing lactate, nicotinamide adenine dinucleotide and an electron transfer agent, and measuring the uptake of oxygen by the oxidation of the resulting NADH with an oxygen-sensing electrode.

Abstract: An improved process for the production of a heteropolysaccharide by fermentation of a methanol containing culture medium with a microorganism of Methylomonas mucosa NRRL B-5696 wherein an alkali metal glycerophosphate is incorporated into the culture medium to provide a source of assimilable phosphate. An iron chelating agent selected from the group consisting of the alkali metal and the ferric/alkali metal salts of ethylenediaminetetraacetic acid may be added to the culture medium and N-tris-(hydroxymethyl)methyl glycine may be employed as a buffering agent. The heteropolysaccharide thus produced may be employed in the in vivo form to provide a viscous aqueous solution injected into a subterranean oil reservoir for mobility control purposes.

Abstract: A method and apparatus for evaporating and condensing moisture from a procession of prepared fruit and vegetable particles at subatmospheric pressures, to manufacture puffed, low-moisture food products.A series of stations of decreasing pressure and increasing temperature is established within the dehydrating apparatus, and the procession of food particles is led and guided by the apparatus to pass systematically from station to station of the series for dehydration treatment. As the particles advance through the series of stations of successively lower pressure, they encounter an oil temperature in each succeeding station which is higher, and a vapor head pressure which is lower, than in the preceding station. By the time leading particles have traversed all stations of the series, they have become puffed and dehydrated.

Abstract: The enhancement of foodstuffs is effected by the addition of a small but effective flavor-modifying amount of a compound having the general formula ##STR1## wherein R.sub.1, R.sub.2 and R.sub.3 are hydrogen, alkyl, alkenyl, aryl or aralkyl provided that R.sub.1, R.sub.2, and R.sub.3 are not all hydrogen.

Abstract: A method for purifying pullulan which comprises cultivating a strain of Aureobasidium pullulans in a liquid medium, removing the cells from the culture broth, precipitating pullulan present in said broth with at least one solvent selected from the group consisting of alcohols, esters and ethers respectively with three or more carbon atoms and ketones with four or more carbon atoms to recover, and then dehydrating and/or drying the recovered pullulan.

Abstract: Process for the determination of at least one of the isoenzymes of lactatedehydrogenase by visually observing and recording the conversion of lactate and nicotinamideadenine-dinucleotide to pyruvate wherein said conversion is carried out at a buffer specific pH value of 6-6.5. The invention also encompasses test reagents for use in making such determinations and also diagnostic aids containing the same for use in detecting abnormal changes in the female genital tract and diagnostic methods utilizing such aids.

Abstract: This disclosure relates to an improved medium for growing organisms (preferably Actinoplanes missouriensis) which produce glucose isomerase. Use of the medium results in increased yields of enzyme in shorter fermentation times. The improved medium contains molasses and particulate soy material, preferably soy flour.

Abstract: A concentrate for fresh lemon-flavored alcohol beverages comprises alcohol and from 0.5 to 10 percent lemon, in the form of lemon peel or lemon oil, is prepared by mixing the lemon and alcohol, and thereafter permitting these ingredients to stand at ambient temperature and form a concentrate solution. Suitable quantities of water and sugar may be added to control the percent alcohol and dryness, respectively, of the beverage.

Abstract: Sweetening compositions which mask the aftertaste of saccharin and potentiate its sweet taste contain saccharin together with an amount of a sweet tasting dipeptide in a quantity effective to mask the aftertaste of saccharin.

Abstract: A method of isomerizing glucose with an enzyme immobilized within a microbial cell has been developed. The immobilized enzyme is prepared by dipping a microbial cell containing glucose isomerase in an aqueous solution containing citric acid and drying it, if necessary. An immobilization of an intracellular enzyme is reinforced by supplemental means such as radiation or others.

Abstract: A method for the detection and the quantitative determination of the enzyme creatine phosphokinase (CPK), an agent present in abnormal concentrational levels in serum in many cases of mental disorders and neuromuscular disorders, and significant in the diagnosis of myocardial infarction. The method utilizes a dried time-stable sample of a pure or mixed component such as a blood-impregnated carrier substance, obviating the need for separatory procedures (i.e., separating the serum from the blood cells) and special handling (i.e. freezing, freeze drying, etc.) to preserve the sample. The mechanism of the laboratory determination is that adenosine diphosphate (ADP) reacts with creatine phosphate in the presence of creatine phosphokinase and magnesium ions to produce adenosine triphosphate (ATP) plus creatine.

Abstract: Microbial cells having active enzymes associated therewith are subjected to flocculation conditions to produce a flocculated whole cell aggregate that is useful in effecting enzyme-catalyzed chemical transformations. Substrates are brought into contact with the flocculated cell aggregates where they undergo chemical transformations in the presence of active enzymes associated with the cells.

Abstract: Microbial cells having active enzymes associated therewith are subjected to flocculation conditions to produce a flocculated whole cell aggregate that .Iadd.is subsequently dried. The dried aggregate .Iaddend.is useful in effecting enzyme-catalyzed chemical transformations. Substrates are brought into contact with the flocculated cell aggregates where they undergo chemical transformations in the presence of active enzymes associated with the cells.