Patents Examined by Alexander H. Spiegler
  • Patent number: 6893847
    Abstract: The present invention provides oligonucleotides for detecting Salmonella toxin gene invA mRNA and stn mRNA which oligonucleotides specifically bind to invA mRNA or stn mRNA at a relatively low temperature (for example, 41° C.) and at a constant temperature, and a process of amplifying Salmonella toxin gene invA mRNA or stn mRNA and a method of detecting the same using the oligonucleotides.
    Type: Grant
    Filed: January 16, 2002
    Date of Patent: May 17, 2005
    Assignee: Tosoh Corporation
    Inventors: Akihiro Yokoyama, Takahiko Ishiguro
  • Patent number: 6884578
    Abstract: The present invention compares expression profiles from matched samples to identify differential gene expression. Samples are matched according to physiological, pharmacological and/or disease state. Comparison of matched samples eliminates gene expression differences that are the result of changes in variables that are not of interest. The gene expression differences that remain can be attributed with a high degree of confidence to the unmatched variation. The gene expression differences thus identified can be used for example to diagnose disease, identify physiological state, design drugs, and monitor therapies.
    Type: Grant
    Filed: December 11, 2000
    Date of Patent: April 26, 2005
    Assignee: Affymetrix, Inc.
    Inventors: Janet A. Warrington, Mamatha Mahadevappa
  • Patent number: 6872530
    Abstract: The present invention relates to a peptide nucleic acid probe-based method for generating data indicative of the presence of a nucleotide polymorphism, mutation, or methylated cytosine in a nucleotide containing compound. A peptide nucleic acid probe (PNAP) is subjected to temperature gradient electrophoresis in the presence of a nucleotide containing compound. The PNAP is irradiated to generate a spectroscopic signal. The spectroscopic signal is converted into data suitable for determining the presence of the nucleotide polymorphism or the mutation in the nucleotide-containing compound.
    Type: Grant
    Filed: April 24, 2002
    Date of Patent: March 29, 2005
    Assignee: SpectruMedix, LLC
    Inventor: Zhaowei Liu
  • Patent number: 6872816
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
    Type: Grant
    Filed: March 24, 1998
    Date of Patent: March 29, 2005
    Assignee: Third Wave Technologies, Inc.
    Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow, Robert W. Kwiatkowski, Stephanie H. Vavra
  • Patent number: 6867021
    Abstract: The present invention provides a multiplex RT-PCR/PCR method, which enables in a single assay the simultaneous detection of any combination of bovine rotavirus, bovine coronavirus, Cryptosporidium parvum, and optionally, Escherichia coli strains producing K99 pili or heat-stable enterotoxin STa.
    Type: Grant
    Filed: February 20, 2001
    Date of Patent: March 15, 2005
    Assignee: Board of Trustees of Michigan State University
    Inventors: Roger K. Maes, Annabel G. Wise
  • Patent number: 6861219
    Abstract: There is a tremendous need for high throughput gene expression technology which can efficiently and cheaply identify and accurately isolate different genes expressed between diseased and normal tissues for use in discovering new drugs. The present invention utilizes a combination of biomolecular chemistry methods to eliminate/degrade redundant sequences and fluorescence dye assay to identify these unique sequences from two cell or tissue populations. cDNA from normal or diseased cells or tissues are hybridized with the RNA of the complement normal or diseased cells or tissues. The hybridized cDNA/RNA is incubated with exonucleases, resulting in degradation of all but the single stranded RNA and DNA. RNA are then eliminated using RNase and the remaining DNA which are unique to the sample are amplified.
    Type: Grant
    Filed: September 24, 2001
    Date of Patent: March 1, 2005
    Assignee: Genexpress Informatics, Inc.
    Inventors: Shahzi Iqbal, Robert Chin
  • Patent number: 6858413
    Abstract: The present invention provides novel isothermal methods of generating multiple copies of, detecting and/or quantifying nucleic acid sequences of interest based on limited primer extension or attachment of oligonucleotide pairs using composite RNA/DNA primers. Methods for generating multiple copies of and/or detecting and/or quantifying nucleic acid sequences, wherein products of primer extension or attachment of oligonucleotide pairs comprising a cleavable portion are generated, and wherein cleavage of the products results in dissociation of cleaved products from target polynucleotides, are provided. The invention further provides compositions, kits and systems for practicing these methods.
    Type: Grant
    Filed: December 13, 2001
    Date of Patent: February 22, 2005
    Assignee: NuGen Technologies, Inc.
    Inventor: Nurith Kurn
  • Patent number: 6846839
    Abstract: The present invention relates to methods for treating diseases and disorders related to unregulated angiogenesis and/or vasculogenesis. More specifically, this invention relates to methods for treating diseases and disorders, such as rheumatoid arthritis, endometriosis, ocular neovascularization, solid tumor growth and metastases, and excessive scarring during wound healing, with indolinone compounds.
    Type: Grant
    Filed: June 16, 1999
    Date of Patent: January 25, 2005
    Assignee: Sugen, Inc.
    Inventors: Peng Cho Tang, Li Sun, Laura Kay Shawver, Klaus Peter Hirth, Annie Fong
  • Patent number: 6822084
    Abstract: Isolated nucleic acid molecules, designated SRT nucleic acid molecules, which encode novel SRT proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SRT nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SRT proteins, mutated SRT proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SRT genes in this organism.
    Type: Grant
    Filed: June 23, 2000
    Date of Patent: November 23, 2004
    Assignee: BASF Aktiengesellschaft
    Inventors: Markus Pompejus, Burkhard Kroger, Hartwig Schröder, Oskar Zelder, Gregor Haberhauer, Heung-Shick Lee, Hyung-Joon Kim
  • Patent number: 6808882
    Abstract: The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having the desired activity using a change in the optical properties of the genetic elements. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
    Type: Grant
    Filed: June 29, 2001
    Date of Patent: October 26, 2004
    Assignee: Medical Research Council
    Inventors: Andrew Griffiths, Dan Tawfik, Armin Sepp
  • Patent number: 6803199
    Abstract: The present invention provides a purified and isolated nucleic acid encoding a sodium/iodide symporter. The present invention also provides purified sodium/iodide symporter, a vector comprising nucleic acid encoding sodium/iodide symporter, a host cell transformed with the vector, and a method for producing recombinant sodium/iodide symporter. In addition, the present invention provides nucleic acid probes and mixtures thereof specific for sodium/iodide symporter nucleic acid and antibodies immunoreactive with sodium/iodide symporter. The present invention also provides a method for diagnosing and treating thyroid disorders associated with non-functional sodium/iodide symporter. Furthermore, the present invention provides a method for the selective ablation of tissue. The present invention also provides a method for identifying an iodide transport protein in non-thyroid tissue. Finally, the present invention provides a non-human, transgenic model for a thyroid disorder.
    Type: Grant
    Filed: November 26, 2001
    Date of Patent: October 12, 2004
    Assignee: Albert Einstein College of Medicine of Yeshiva University
    Inventors: Nancy Carrasco, Ge Dai, Orlie Levy
  • Patent number: 6780587
    Abstract: Methods and compositions are provided for diagnosing and treating Pseudoxanthoma elasticum (PXE) patients and PXE carriers. Methods and compositions are based on the discovery that PXE mutations are located in the MRP6 (ABCC6) gene.
    Type: Grant
    Filed: February 23, 2001
    Date of Patent: August 24, 2004
    Assignees: PXE International, Inc., The University of Hawaii
    Inventors: Charles D. Boyd, Katalin Csiszar, Olivier LeSaux, Zsolt Urban, Sharon Terry
  • Patent number: 6780636
    Abstract: The present invention provides a method for creating a cryoarray of frozen tissue cores using a cryoarray system. Such system comprises a tissue mold, an embedding medium and a cryoarray device. The cryoarray device comprises a mold plate, an ejector plate, mold alignment pins, ejector pins, and cryoarray pins. Such method/system may be used for preparing frozen sections with multiple tissue specimens for assays such as in-situ hybridization and immunohistochemistry.
    Type: Grant
    Filed: December 10, 2001
    Date of Patent: August 24, 2004
    Assignee: Sloan Kettering Institute for Cancer Research
    Inventors: Stephen Mastorides, Carlos Cordon-Cardo
  • Patent number: 6770437
    Abstract: This invention provides methods of assigning an individual to a population of origin based on statistical analyses of the individual's genotype and the genetic architecture of the underlying populations from which the individual may have originated.
    Type: Grant
    Filed: November 9, 2000
    Date of Patent: August 3, 2004
    Assignee: ViaGen, Inc.
    Inventors: Jeremy Francis Taylor, Sara L. F. Davis, Luke Lind, Scott K. Davis
  • Patent number: 6767704
    Abstract: Screening and diagnostic reagents, kits and methods for primary and/or metastatic stomach or esophageal cancer are disclosed. Compositions for and methods of imaging and treating primary and/or metastatic stomach or esophageal cancer are disclosed. Vaccines compositions and methods of for treating and preventing primary and/or metastatic stomach or esophageal cancer are disclosed.
    Type: Grant
    Filed: March 27, 2001
    Date of Patent: July 27, 2004
    Assignee: Thomas Jefferson University
    Inventors: Scott A. Waldman, Jason Park, Stephanie Schulz
  • Patent number: 6706499
    Abstract: A medium is disclosed for embedding and preserving single cells or a cell tissue for an extended period of time at a temperature not exceeding 0° C. in a state suitable for DNA and/or RNA amplification, comprising an aqueous solution of at least one water-soluble cellulose derivative and, optionally, an osmotic pressure stabilizing agent. A method for preserving single cells or a cell tissue for an extended period of time at a temperature not exceeding 0° C. in a state suitable for DNA and/or RNA amplification using the above medium is also disclosed.
    Type: Grant
    Filed: November 13, 2001
    Date of Patent: March 16, 2004
    Assignee: Ascendia AB
    Inventors: Per Månsson, Tomas Lundin
  • Patent number: 6699676
    Abstract: Longterm elevation of the intracellular Na+/K+ ratio inhibits macromolecule synthesis and proliferation in the majority of cell types studied so far, including vascular smooth muscle cells (VSMC). We report here that inhibition of the Na+,K+ pump in VSMC by ouabain or 1 hour preincubation in K+-depleted medium attenuated apoptosis triggered by serum withdrawal, staurosporine or okadaic acid. In the absence of ouabain, both DNA degradation and caspase-3 activation in VSMC undergoing apoptosis were insensitive to modification of the extracellular Na+/K+ ratio as well as to hyperosmotic cell shrinkage. In contrast, protection of VSMC from apoptosis by ouabain was abolished under equimolar substitution of Na+o with K+o, showing that the anti apoptotic action of Na+,K+ pump inhibition was caused by inversion of the intracellular Na+/K+ ratio.
    Type: Grant
    Filed: June 2, 2000
    Date of Patent: March 2, 2004
    Assignee: Corporation du Centre de Recherche du Centre, Hospitalier du L′Universite de Montreal
    Inventors: Sergei N. Orlov, Pavel Hamet, Johanne Tremblay
  • Patent number: 6696254
    Abstract: The invention provides probes, antibodies and methods for detecting a gene that is only found in Enterobacteriaceae, the deoxyguanosine triphosphate triphosphohydrolase gene. These probes and methods are useful for the detecting whether test samples, including food and water samples, are infected with enteric bacteria.
    Type: Grant
    Filed: November 21, 2001
    Date of Patent: February 24, 2004
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventor: Stephen Quirk
  • Patent number: 6686155
    Abstract: The present invention relates to prognostic methods which are useful in medicine, particularly cancer chemotherapy. The object of the invention to provide a method for assessing GST-pi expression levels in fixed or fixed and paraffin embedded tissues and prognosticate the probable resistance or sensitivity of a patient's tumor to treatment with platinum-based therapies by examination of the amount of GST-pi mRNA in a patient's tumor cells and comparing it to a predetermined threshold expression level. More specifically, the invention provides to oligonucleotide primer pair GST-pi and methods comprising their use for detecting levels of GST-pi mRNA.
    Type: Grant
    Filed: June 14, 2001
    Date of Patent: February 3, 2004
    Assignee: Response Genetics, Inc.
    Inventor: Kathleen D. Danenberg
  • Patent number: 6680427
    Abstract: This invention relates to an isolated nucleic acid fragment encoding an Mlo-like polypeptide. The invention also relates to the construction of a chimeric gene encoding all or a portion of the Mlo-like polypeptide, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the Mlo-like polypeptide in a transformed host cell.
    Type: Grant
    Filed: March 24, 2000
    Date of Patent: January 20, 2004
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Rebecca E. Cahoon, Guo-Hua Miao, J. Antoni Rafalski, Graziana Taramino