Patents Examined by Bradley Sisson
  • Patent number: 5998577
    Abstract: A method of determining the sequence of monomers which is a conformational equivalent of an epitope which is complementary to a particular paratope of an antibody of interest, the method comprising the steps of: 1. synthesizing a plurality of catamer preparations; each of said catamer preparations consisting of a plurality of catamers in which the composition at one or more designated positions in each catamer is known, and the composition at the remaining positions is randomly made up from members of a defined set of monomers; and said plurality of catamer preparations comprising preparations in which the composition at the designated positions is systematically varied to contain members from a defined set of monomers; 2. contacting each of the plurality of catamer preparations with the antibody of interest; and 3.
    Type: Grant
    Filed: October 29, 1992
    Date of Patent: December 7, 1999
    Assignee: Chiron Mimotopes Pty. Ltd.
    Inventor: Hendrik M. Geysen
  • Patent number: 5989886
    Abstract: A new type of dominant negative gene-fusion acts as an antiviral agent when expressed in infected cells. The dominant negative effect is obtained by fusing a gene coding for a capsid or envelope protein of a virus to a gene coding for an enzyme (e.g., nuclease or protease) that can destructively hydrolyze or modify the encapsulated viral DNA, RNA or proteins. The fusion gene is expressed in virally infected cells; viruses assembled in these cells will then "self-destruct" because the fusion enzyme incorporated in the viral particles destroys or functionally alters essential components of the virus, thus preventing the virus from productively infecting another cell.
    Type: Grant
    Filed: January 2, 1991
    Date of Patent: November 23, 1999
    Assignee: The Johns Hopkins University
    Inventors: Jef D Boeke, Georges Natsoulis
  • Patent number: 5989893
    Abstract: The invention discloses a chimeric GLUT transporter including a GLUT receptor polypeptide fused to a domain of a detectable heterologous polypeptide and cells expressing such reporter constructs. Cells expressing a detectably-tagged chimeric GLUT transporter are used in methods of screening candidate compounds for their ability to agonize or antagonize an interaction between a ligand and a receptor, e.g., insulin. In addition, a 30 amino acid intracellular retention sequence is disclosed.
    Type: Grant
    Filed: August 8, 1994
    Date of Patent: November 23, 1999
    Assignee: University of Massachusetts Medical Center
    Inventors: Michael P. Czech, Silvia Corvera
  • Patent number: 5986067
    Abstract: Recombinant transferrin, non-glycosylated recombinant transferrin, transferrin half-molecules and mutant transferrins having altered metal-binding or other properties are described. The recombinant transferrin molecules are expressed in functional form by stable eukaryotic cell lines such as baby hamster kidney cells transformed with an expression vector encoding the recombinant molecule. The recombinant transferrins can be used in metal chelation therapy to bind and clear excess toxic metals in patients suffering from metal overloads or as tissue culture medium supplements or replacements.
    Type: Grant
    Filed: December 28, 1993
    Date of Patent: November 16, 1999
    Assignees: The University of Vermont and State Agricultural College, The University of British Columbia
    Inventors: Walter D. Funk, Ross T. A. MacGillivray, Anne B. Mason, Robert C. Woodworth
  • Patent number: 5603898
    Abstract: A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.
    Type: Grant
    Filed: December 17, 1992
    Date of Patent: February 18, 1997
    Assignees: Fujirejiro Inc., Fuji Photo Film Co., Ltd.
    Inventors: Yoshihiro Ashihara, Yukio Sudo, Isao Nishizono, Toshikage Hiraoka, Tetsuji Tanimoto, Shigeki Kageyama
  • Patent number: 5064770
    Abstract: An immunoassay assay for 1,25-dihydroxy vitamin D receptor protein (3) is disclosed. It uses two antibodies (1) (7), one labelled (2) and one anchored with biotin (6). The two antibodies bind to different epitopes (4) (5) of the receptor (3) provided, and the biotin (6) permits use of avidin sepharose (8) to separate labelled receptor protein for measurement.
    Type: Grant
    Filed: August 1, 1989
    Date of Patent: November 12, 1991
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Hector F. DeLuca, Maria E. Sandgren