Abstract: The present invention relates to the method of using a composition of matter that maintains the biophysical and physiological characteristics of natural surfactant, including the rapid and spontaneous adsorption of dilute suspensions to an air-liquid interface; the generation of low surface tension (<2.1 mN/m) at a surface area compression of 50% by the pulsating bubble method of Enhorning; and the restoration of greater than 75% of the lost volume of a surfactant deficient lung as determined by the method of Bermel. The material is produced by adding disaturated phospholipid to the extract of surface active material obtained by lavage of the air space of the lungs of animals. The material contains 90-96% phospholipid, 0-6% cholesterol, and 1-3% protein. Greater than 99% of the protein is lung surfactant apoproteins B & C. A sterile pharmaceutical preparation of the surface active material can be used for treating diseases caused by deficiency or dysfunction or natural lung surfactant.

Abstract: The present invention relates to novel immortalized precursor cell populations derived from embryonic stem cell populations and methods to produce such cell populations. Also disclosed is an assay to identify regulatory compounds capable of controlling cell growth for therapeutic and experimental use.

Abstract: Novel clear solutions or gels containing a volatile hydrocarbon, surfactants and a coupling agent do not separate, but rather due to solubilization of the volatile hydrocarbon by the coupling agent, provide acceptable consumer products.

Abstract: The invention relates to a process for manufacturing injection moulded objects of thermoplastic material by injecting molten thermoplastic material into a mould from at least two distinct injection points whereas the flows of molten material from each injection point each fill part of the mould and flow together to fill the entire mould while still in molten state, wherein objects having a size of more than 80 cm along each of its three dimensional axes are produced by providing at least two distinct injection points at opposite sides of the mould. The invention also relates to injection moulding equipment for the manufacture of moulded objects of thermoplastic material, comprising at least two distinct injection points provided at opposite sides of a single mould assembly. The invention further also relates to threedimensional moulded objects of thermoplastic material having a size of more than 80 cm along each of its threedimensional axes.

Abstract: A reticle is designed with a method including a step of generating first dummy patterns with intervals from main patterns. Each of the first dummy patterns are divided into a plurality of spaced apart second dummy patterns and then each of the second dummy patterns are measured to find third dummy patterns having widths and areas below smallest allowable values. The third dummy patterns are then respectively connected to second dummy patterns which are adjacent to the third dummy patters by generating a connecting dummy pattern. Selective non-connected third dummy patterns are removed. The first dummy patterns are divided into a plurality of second dummy patterns by vertical and horizontal strip lines crossing the first dummy patterns.

Abstract: The invention comprises the nucleotide sequences comprising the FIPV S gene, or a fragment of this gene, which are modified in at least one of the antigenic regions A1 and A2 which are involved in enhancement, as well as the use of these sequences for the expression of modified proteins, and for the construction of recombinant viruses or expression plasmids, and the use of the recombinant viruses as vaccines against feline infectious peritonitis, the use of the expression plasmids as immunizing composition by direct injection of the said plasmids into cats, and the use of the modified proteins as vaccine.

Abstract: A monoclonal antibody specifically recognizing adeno-associated virus CAP protein, which is produced by hybridomas obtained by fusing lymphocytes prepared from a mammal which has been immunized with the adeno-associated virus CAP protein or a recombinant thereof as an antigen with a myeloma cell line. The monoclonal antibody of the present invention is a novel antibody and capable of specifically recognizing the adeno-associated virus CAP protein. Thus, it is applicable to the detection of the adeno-associated virus and the purification of adeno-associated virus vectors for gene therapy.

Abstract: A method of increasing the relative number of CD34.sup.+ cells in a cell population is described. The method comprises contacting the cell population with an agent that causes the relative number of CD34.sup.+ cells to increase.

Abstract: A process for the continuous preparation of a coated, long fiber reinforcing composite structure suitable for the preparation of shaped articles. The process is characterized by impregnating a plurality of continuous lengths of reinforcing fiber strands with a first thermoplastic resin material while continuously drawing the fiber strands to produce a long fiber reinforcing composite structure followed by coating a second thermoplastic resin material containing additives onto the long fiber reinforcing composite structure to produce a coated, long fiber reinforcing composite structure. The coating additives may be selected from mineral reinforcing agents, lubricants, flame retardants, blowing agents, foaming agents, ultraviolet light resistant agents, heat sensitive pigments, etc. The coated, long fiber reinforcing composite structure prepared according to the process is suitable for the preparation of shaped articles exhibiting good physical and chemical properties.

Abstract: An aqueous or aqueous/alcoholic cosmetic composition comprising an aqueous dispersion of insoluble particles of a non-ionic film-forming polymer, the polymer particles being at a concentration greater than 15% by weight with respect to the total weight of the composition and the glass transition temperature of the polymer particles of the composition ranging from 15 to 35.degree. C. and a process for the cosmetic treatment of keratinous substances using these compositions.

Abstract: This invention is directed to novel nucleophilic polysubstituted aryl acridinium conjugates and the methods for preparation thereof. The novel nucleophilic polysubstituted aryl acridinium conjugates are useful in biological assays, including novel assays for the determination of Vitamin B.sub.12, folate, cortisol, estradiol, and thromboxane B.sub.2.

Abstract: The invention comprises the nucleotide sequences comprising the FIPV S gene, or a fragment of this gene, which are modified in at least one of the antigenic regions A1 and A2 which are involved in enhancement, as well as the use of these sequences for the expression of modified proteins, and for the construction of recombinant viruses or expression plasmids, and the use of the recombinant viruses as vaccines against feline infectious peritonitis, the use of the expression plasmids as immunizing composition by direct injection of the said plasmids into cats, and the use of the modified proteins as vaccine.

Abstract: A process of parthenogenic activation of mammalian oocytes which includes increasing intracellular levels of divalent cations in the oocyte; and reducing phosphorylation of cellular proteins in the oocyte. One method of accomplishing this is by introducing Ca.sup.2+ free cation, such as ionomycin, to the oocyte and then preventing phosphorylation of the cellular proteins within the oocyte by adding a serine-threonine kinase inhibitor, such as 6-dimethylaminopurine (DMAP).

Abstract: This invention relates to a denture adhesive composition comprising a lower alkyl vinyl ether-maleic acid, anhydride, or salt polymer or mixtures thereof, one or more metallic salts, nonionically associated with the lower alkyl vinyl ether-maleic acid, anhydride, or salt polymer, and at least one non-adhesive self-supporting layer. This invention may also comprise other adhesive components.

Abstract: Moisturizing liquid personal cleansing emulsion compositions which comprise a moisturizing phase and an aqueous cleansing phase are disclosed. The moisturizing phase comprises from about 1% to about 30% by weight of the composition of lipophilic skin moisturizing agents comprised of droplets having a particle size distribution such that at least about 10% by weight of the droplets have a diameter of at least about 200 microns. The aqueous cleansing phase comprises from about 0.1% to about 10% by weight of the composition of a stabilizer, from about 5% to about 30% by weight of the composition of a lathering surfactant, and water. The liquid personal cleansing emulsion compositions contain less than about 5% by weight of fatty acid soap.

Abstract: Disclosed herein are methods for vaccinating mammals against parvovirus. In one form, a recombinant, replication incompetent vector containing DNA coding for canine parvoviral capsid protein is administered to a dog while the pup's maternal antibodies are still functioning. This acts to prime T-helper cells. A second administration of either canine parvoviral capsid protein, or a construct capable of expressing it, is then administered. The latter administration immunizes the mammal notwithstanding the maternal antibody effect.

Abstract: The invention relates to methods and compositions for inhibition of viral replication in animal cells. In particular, inhibition of viral replication in a target cell is achieved by introducing into the cell (1) a protein which can be incorporated along with wild type nucleocapsid subunits into a viral nucleocapsid assembling within the cell, and thereby renders the nucleocapsid deficient in encapsidating viral nucleic acid; or (2) a recombinant nucleic acid construct that directs overexpression of the protein.

Abstract: A process for preparing a culture skin which comprises the following steps:a) a step in which a culture skin matrix comprising a collagen sponge, a collagen sheet or a collagen gel is prepared in a means having a projection to provide said matrix with a penetrating pore,b) a step in which a skin-derived cell is seeded and cultured on said matrix, andc) a step in which the culture skin is provided with a penetrating pore by the means having the projection before said culture skin covers a skin defect, if necessary, the steps a) and b) are simultaneously carried out; a culture skin matrix and a culture skin obtained thereby.

Abstract: This invention is directed to metal-matrix composites which include a substantially continuous phase of metal and reinforcing ceramic particulate substantially uniformly dispersed therein and comprising at least two of barium titanium, titanium dioxide, and titanium nitride. The composite may include other reinforcing ceramic particulate materials like metal carbides such as titanium carbide and other titanates like calcium titanate. The reinforcing particulate can comprise up to about 70 volume percent of the composite and have an average particle diameter of between about 0.1 micron and 100 microns. In forming the composite, the metal powder employed has an average particle diameter between about 1 and 20 microns. The composite is useful to manufacture, e.g., automotive parts such as brake rotors and structural components.

Abstract: The present invention relates to a new use of N,S-diacetylcysteine ethyl ester for the preparation of a pharmaceutical composition for treating virus-induced disease. In particular, the invention concerns the use of DACEE for the preparation of a pharmaceutical composition for treating virus-induced disease, the DACEE used destroying disulfide bridges present in viral proteins.