Abstract: The present invention relates in general to the field of recombinant protein expression. In particular, the present invention relates to a method for selecting a suitable candidate cell clone for recombinant protein expression and to a host cell for recombinant protein expression, the host cell exhibiting artificially modified gene expression of at least one gene selected from the group consisting of: Fkbp10, ZdhhC6, Myrip, Actc1, AC124993.19, Runx2, AC158560.4, PlekhB1, Rps6KA2, Sept1, Sprr2k, and Flt1.
Type:
Grant
Filed:
November 12, 2015
Date of Patent:
February 9, 2021
Assignee:
Lek Pharmaceuticals d.d.
Inventors:
Uro{hacek over (s)} Jamnikar, Marjanca Blas, Kristina Gruden
Abstract: A method of making a polypeptide including at least one covalent bond between a pair of reactive side chains of corresponding amino acids, wherein the covalent bond is insensitive to reduction is provided including genetically modifying a genomically recoded organism to express a corresponding synthetase, tRNA or synthetase/tRNA pair for translating mRNA encoding the corresponding amino acids having the reactive side chains into the polypeptide and to express the polypeptide including the at least one pair of the reactive side chains wherein the reactive side chains are oriented near one another when the expressed polypeptide is in a folded configuration, wherein the reactive side chains react to form the covalent bond that is insensitive to reduction.
Type:
Grant
Filed:
October 28, 2015
Date of Patent:
February 2, 2021
Assignee:
President and Fellows of Harvard College
Inventors:
George M. Church, Christopher J. Gregg, Marc J. Lajoie, Daniel J. Mandell
Abstract: The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.
Type:
Grant
Filed:
December 3, 2015
Date of Patent:
January 26, 2021
Assignee:
AGILENT TECHNOLOGIES, INC.
Inventors:
Daniel E. Ryan, Douglas J. Dellinger, Jeffrey R. Sampson, Robert Kaiser, Joel Myerson
Abstract: It relates to a method for preparation of four kinds of 2?, 3?-cNMPs (2?, 3?-cAMP, 2?, 3?-cGMP, 2?, 3?-cCMP and 2?, 3?-cUMP), comprising steps of: (1) extract genomic DNA and amplify gene If3; (2) ligate If3 gene to expression plasmid to construct a recombinant vector, and transfer the recombinant vector to E. coli to obtain a recombinant strain. Cultivate the recombinant strain and collect the fermentation broth; (3) collect the cells form the fermentation broth and disrupt the cells, and then purify the recombinant protein IF3 from the cell extract by Ni2+-nitrilotriacetic acid resin. Incubate the recombinant protein IF3 solution at 0° C. for 3 days to release 2?, 3?-cNMPs from IF3, and centrifuge the solution; (4) Ultrafiltrate the supernatant to remove proteins, and prepare four kinds of 2?, 3?-cNMPs by high-performance liquid chromatographic (HPLC) on a C18 reversed-phase column.
Type:
Grant
Filed:
October 24, 2016
Date of Patent:
January 5, 2021
Assignee:
SHANDONG UNIVERSITY
Inventors:
Xiulan Chen, Yuzhong Zhang, Ang Liu, Yang Yu, Binbin Xie, Qilong Qin, Xiaoyan Song, Mei Shi
Abstract: A portable system for extracting, optionally amplifying, and detecting nucleic acids or proteins using a compact integrated chip in combination with a mobile device system for analyzing detected signals, and comparing and distributing the results via a wireless network. Related systems and methods are provided.
Abstract: This document provides methods and materials involved in cloning functional TCRs from single T cells. For example, methods and materials for obtaining nucleic acid encoding a TCR from a single T cell and arranging that nucleic acid to form nucleic acid vectors successfully designed to express a TCR, kits for obtaining nucleic acid encoding a TCR from a single T cell and arranging that nucleic acid to form nucleic acid vectors successfully designed to express a TCR, methods for making such kits, collections of nucleic acid primers designed to amplify the entire coding sequence of both variable regions for each expressed V segment for functional ?? or ?? TCRs of a particular mammalian species, methods for using such collections of nucleic acid primers to clone functional TCRs from single T cells, and kits containing such collections of nucleic acid primers to clone functional TCRs from single T cells are provided.
Type:
Grant
Filed:
November 29, 2017
Date of Patent:
December 29, 2020
Assignee:
University of Pittsburgh—Of the Commonwealth System of Higher Education
Inventors:
Mark Shlomchik, Adriana Turqueti Neves, Eduardo Schittler Neves, Constantinos George Panousis, Alexander McIntyre Rowe
Abstract: A system for expression-based discrimination of distinct clinical disease states in prostate cancer is provided that is based on the identification of sets of gene transcripts, which are characterized in that changes in expression of each gene transcript within a set of gene transcripts can be correlated with recurrent or non-recurrent prostate cancer. The Prostate Cancer Prognostic system provides for sets of “prostate cancer prognostic” target sequences and further provides for combinations of polynucleotide probes and primers derived there from. These combinations of polynucleotide probes can be provided in solution or as an array. The combination of probes and the arrays can be used for diagnosis. The invention further provides further methods of classifying prostate cancer tissue.
Abstract: The present invention relates to a method to produce mutated microorganisms which resist the phenomenon of lactose killing and to the microorganisms obtainable via said method. Such engineered microorganisms can be applied for the production of specialty products, such as but not limited to specialty carbohydrates, glycolipids and galactosylated compounds.
Type:
Grant
Filed:
November 12, 2015
Date of Patent:
December 8, 2020
Assignee:
INBIOSE N.V.
Inventors:
Joeri Beauprez, Sofie De Maeseneire, Eric Timmermans
Abstract: The present invention relates to scaffold proteins derived from plant cystatins and to nucleic acids encoding them. The scaffolds are highly stable and have the ability to display peptides. The scaffolds are particularly well suited for constructing libraries, e.g., in phage display or related systems. The invention also relates to various uses of the scaffolds, including in therapy, diagnosis, environmental and security monitoring, synthetic biology and research, and to cells and cell cultures expressing the scaffold proteins.
Abstract: The present invention relates to a method for improving an industrial yeast strain of industrial interest, particularly a sterile hybrid strain, without resorting to recombinant DNA techniques.
Type:
Grant
Filed:
November 29, 2013
Date of Patent:
November 24, 2020
Assignees:
INSTITUT CURIE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6), UNIVERSITE NICE SOPHIA ANTIPOLIS, INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE)
Abstract: The invention provides a novel cell line development method useful to screen for recombinant protein production. The method utilizes a membrane-anchored reporter or an intracellular reporter residing in the expression vector for a gene of interest to facilitate initial cell selection by FACS or MACS. A switching mechanism can be used to delete the reporter from the chromosome by providing an appropriate DNA recombinase, which turns the selected cells into production cells that secrete the protein of interest without co-expression of the reporter.
Abstract: The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.
Type:
Grant
Filed:
June 24, 2016
Date of Patent:
October 20, 2020
Assignee:
AMYRIS, INC.
Inventors:
Penelope R. Chua, Hanxiao Jiang, Adam Leon Meadows
Abstract: The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR systems, components, and methods for targeted modification of nucleic acids such as DNA. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.
Type:
Grant
Filed:
November 11, 2019
Date of Patent:
October 20, 2020
Assignee:
ARBOR BIOTECHNOLOGIES, INC.
Inventors:
Shaorong Chong, Winston X. Yan, David A. Scott, David R. Cheng, Pratyusha Hunnewell
Abstract: The present invention relates to a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a pathogenic antigen or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic acid for increasing the expression of said encoded peptide or protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g. for use in the treatment of infectious diseases. The present invention further describes a method for increasing the expression of a peptide or protein comprising a pathogenic antigen or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.
Type:
Grant
Filed:
February 8, 2018
Date of Patent:
October 13, 2020
Assignee:
CureVac AG
Inventors:
Andreas Thess, Thomas Schlake, Jochen Probst
Abstract: A method for determining an interaction between a first protein and a second protein comprises the steps of: expressing in a cell or introducing into a cell a first fusion protein comprising the first protein, a multimerizable protein, and a fluorescent protein, and a second fusion protein comprising the second protein and a multimerizable protein; detecting a fluorescent focus formed by an association between the first fusion protein and the second fusion protein in the cell; and determining an interaction between the first protein and the second protein according to the detection of the fluorescent focus.
Type:
Grant
Filed:
June 10, 2015
Date of Patent:
September 1, 2020
Assignee:
MEDICAL & BIOLOGICAL LABORATORIES CO., LTD.
Abstract: Provided are a human type 55 replication defective adenovirus vector, a method for preparing the same and uses thereof. The human type 55 replication defective adenovirus vector is prepared by the following method: knocking out E1 and E3 genes from Ad55, substituting the open reading frame 6 or the open reading frames 2, 3, 4, 6, and 6/7 of E4 gene in Ad55 genome with the corresponding open reading frames of Ad5 genome. In addition, an exogenous gene expression cassette may also be integrated into the E1 gene region of Ad55.
Abstract: Disclosed are transformed cells comprising one or more genetic modifications that increase the lipid content of the cell, e.g., relative to an unmodified cell of the same type. Also disclosed are methods for increasing the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the cell.
Type:
Grant
Filed:
December 29, 2015
Date of Patent:
September 1, 2020
Assignee:
NOVOGY, INC.
Inventors:
Arthur J. Shaw, IV, Johannes Pieter Van Dijken, Annapurna Kamineni, Jonathan Friedlander, Vasiliki Tsakraklides, Maureen Hamilton, Elena E. Brevnova
Abstract: A method for producing a protein of interest on a manufacturing scale is based on integration, by homologous recombination, of the DNA encoding the protein of interest into a bacterial cell's genome at a pre-selected site. The manufacturing scale production of recombinant proteins is in the fed-batch mode, semi-continuous or in a chemostat.
Type:
Grant
Filed:
May 12, 2017
Date of Patent:
August 25, 2020
Assignees:
Boehringer Ingelheim RCV GmbH & Co KG, Sandoz AG
Inventors:
Gerald Striedner, Johann Huber, Daniela Reinisch
Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.