Patents Examined by Che S. Chereskin
  • Patent number: 5639952
    Abstract: A plant chlorophyll a/b binding (Cab) protein gene was isolated from a maize genomic library. The promoter/regulatory system of this gene actively functions in controlling Cab gene expression under conditions of darkness and in enhancing gene expression under conditions of light. This invention contemplates the utilization of the Cab promoter/regulatory system in regulating non-homologous structural gene expression in transgenic plants under conditions of darkness followed by further enhancement of gene expression under conditions of light.
    Type: Grant
    Filed: April 7, 1995
    Date of Patent: June 17, 1997
    Assignee: Mycogen Plant Science, Inc.
    Inventors: Peter H. Quail, Thomas D. Sullivan, Alan H. Christensen
  • Patent number: 5635480
    Abstract: Disclosed and claimed are toxins produced by novel Bacillus thuringiensis isolates designated B.t. PS92J, B.t. PS196S1, B.t. PS201L1, and B.t. PS201T6, which have dipteran and/or corn rootworm activity. Thus, the isolates, or mutants thereof, can be used to control such pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.
    Type: Grant
    Filed: May 31, 1995
    Date of Patent: June 3, 1997
    Assignee: Mycogen Corporation
    Inventors: Jewel Payne, Kenneth E. Narva, Kendrick A. Uyeda, Christine J. Stalder, Tracy E. Michaels
  • Patent number: 5633444
    Abstract: Plant cells are transformed by:(i) transforming a plant cell whose growth is sensitive to inhibition by a sulfonamide or a salt thereof with a chimaeric gene comprising (a) a plant promoter, (b) a sulfonamide resistance gene having a sequence encoding a transit peptide fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence;(ii) selecting a transformed plant cell whose growth is resistant to inhibition by a sulfonamide or salt thereof;(iii) optionally, regenerating from the transformed plant cell a genetically transformed plant which exhibit the said resistance;(iv) optionally, obtaining seed from the regenerated plant; and(v) optionally, propagating plants from the seed.The growth of weeds can be controlled by a locus where a transgenic plant obtained as above is being cultivated by applying to the locus an effective amount of a herbicide, such as asulam, which acts by inhibiting dihydropteroate synthase.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: May 27, 1997
    Assignee: Rhone-Poulenc Agrochimie Limited
    Inventors: Jean F. Guerineau, Philip M. Mullineaux, Edgar W. Parnell
  • Patent number: 5633449
    Abstract: The present invention describes a method for the induction of resistance in a plant host to a RNA or DNA virus pathogenic to the plant which comprises isolating a fragment of viral RNA or DNA associated with the replicase portion of the virus genome, specifically a portion that does not involve a read-through portion of the gene, and integrating a DNA copy of the isolated fragment or a portion thereof into the genome of a recipient plant in such a manner that the plant becomes transformed with the inserted fragment.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: May 27, 1997
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Milton Zaitlin, Peter Palukaitis
  • Patent number: 5632987
    Abstract: Disclosed and claimed are toxins and genes from Bacillus thuringiensis strains designated PS80JJ1, PS158D5, PS167P, PS169E, PS177F1, PS177G, PS204G4, PS204G6 which can be used to control corn rootworm. Mutants which retain the activity of the parent strain can be used to control the pest. Further, isolated spores or purified toxins from these isolates can be used to control corn rootworm. Genes encoding .delta.-endotoxins can be removed from these strains using standard well-known techniques, and transferred to other hosts. Expression of the .delta.-endotoxin in such hosts results in control of corn rootworm larvae.
    Type: Grant
    Filed: December 16, 1994
    Date of Patent: May 27, 1997
    Assignee: Mycogen Corporation
    Inventors: Jewel Payne, Kenneth E. Narva
  • Patent number: 5633435
    Abstract: Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
    Type: Grant
    Filed: September 13, 1994
    Date of Patent: May 27, 1997
    Assignee: Monsanto Company
    Inventors: Gerard F. Barry, Ganesh M. Kishore, Stephen R. Padgette, William C. Stallings
  • Patent number: 5629183
    Abstract: Plant germplasm is transformed with foreign DNA by introducing the DNA into pollen grains by a technique such as electroporation, mating ova of the desired plant line with the transformed pollen, and selecting for the transformed germplasm. The germinating pollen, resultant seed, and the progeny can each be screened for expression of the foreign gene. The transformed pollen can be used as a vector for introducing the foreign DNA into plant lines of similar or dissimilar origin, including both monocots and dicots.
    Type: Grant
    Filed: November 28, 1994
    Date of Patent: May 13, 1997
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: James A. Saunders, Benjamin F. Matthews
  • Patent number: 5628995
    Abstract: A method to combat or control Ostrinia nubilalis by contacting such insects with a CryIB protein or a combination of a CryIB protein and a CryIAb or CryIAc protein.
    Type: Grant
    Filed: January 25, 1995
    Date of Patent: May 13, 1997
    Assignee: Plant Genetic Systems N.V.
    Inventors: Marnix Peferoen, Stefan Jansens, Peter Denolf
  • Patent number: 5627061
    Abstract: Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: May 6, 1997
    Assignee: Monsanto Company
    Inventors: Gerard F. Barry, Ganesh M. Kishore, Stephen R. Padgette, William C. Stallings
  • Patent number: 5618988
    Abstract: A transgenic higher plant, seed containing the transgene, and methods of providing enhanced carotenoid accumulation are disclosed. The transgenic higher plant has a genomic structural gene that encodes a chimeric polypeptide conjugate and over accumulates a colored native carotenoid in a preselected storage organ relative to the accumulation in a non-transgenic plant of the same type. Expression of the chimeric polypeptide is driven by a promoter operatively linked to that structural gene that provides storage organ-enhanced expression. The chimeric polypeptide has an N-terminal plastid transit peptide portion whose C-terminus is linked to the N-terminus of a non-higher plant phytoene synthase enzyme.
    Type: Grant
    Filed: October 28, 1994
    Date of Patent: April 8, 1997
    Assignee: Amoco Corporation
    Inventors: Randal Hauptmann, William H. Eschenfeldt, Jami English, Friedhelm L. Brinkhaus
  • Patent number: 5612471
    Abstract: The present invention provides nucleic sequences from genes which are preferentially expressed in feeding site cells. These sequences can be used to produce transgenic plants resistant to nematode infection.
    Type: Grant
    Filed: May 25, 1994
    Date of Patent: March 18, 1997
    Assignee: The Regents of the University of California
    Inventors: David McK. Bird, Mark A. Wilson
  • Patent number: 5612191
    Abstract: Genes controlling gibberellic biosynthesis are used in genetic engineering to alter plant development. Alterations in the nature or quantity of products of the genes affects plant development. A family of genes in monocots encodes a cyclase involved in the early steps of gibberellic acid (GA) biosynthesis. A member of the family, the gene An1, is identified in maize and cloned and the function of the gene is characterized. Using recombinant genetic technology, GA levels are manipulated. Changes in GA levels alter monocot plant phenotypes, for example, height and fertility.
    Type: Grant
    Filed: June 17, 1994
    Date of Patent: March 18, 1997
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Steven P. Briggs, Robert J. Bensen
  • Patent number: 5608142
    Abstract: A plant expression vector is constructed to cause the expression of an amino-terminal portion of the Bacillus thuringiensis delta-endotoxin gene in plant cells and the vector is used to create transgenic plants expressing the toxin. A truncated form of the toxin is used, with carboxy-terminal prolines added for stability. A translational enhancer sequence derived from the untranslated leader sequence from the mRNA of the coat protein gene of alfalfa mosaic virus coat protein gene is placed between a promoter and the toxin gene to increase translational efficiency. The transgenic plants produced are toxic to Lepidopteran pests and can transmit that trait to their progeny by normal Mendelian inheritance.
    Type: Grant
    Filed: January 23, 1989
    Date of Patent: March 4, 1997
    Assignee: Agracetus, Inc.
    Inventors: Kenneth A. Barton, Paul F. Umbeck
  • Patent number: 5608143
    Abstract: The preparation and use of nucleic acid promoter fragments derived from several genes from corn, petunia and tobacco which are highly responsive to a number of substituted benzenesulfonamides and related compounds are described. These promoter fragments are useful in creating recombinant DNA constructions comprising nucleic acid sequences encoding any desired gene product operably linked to such promoter fragments which can be utilized to transform plants and bring the expression of the gene product under external chemical control in various tissues of monocotyledonous and dicotyledonous plants.
    Type: Grant
    Filed: July 25, 1994
    Date of Patent: March 4, 1997
    Assignee: E. I. Du Pont de Nemours and Company
    Inventors: Howard P. Hershey, Carol D. Katayama, Edward J. Ralston, Timothy D. Stoner, James F. Wong
  • Patent number: 5602322
    Abstract: The present invention is directed to nucleic acid sequences for constitutive triple response mutants and corresponding amino acid sequences. Several ctr mutations are included within the scope of the present invention. The nucleic acid sequences set forth in SEQUENCE ID NUMBERS 1, and 3-6 as well as amino acid sequences set forth in SEQUENCE ID NUMBERS 1 and 2 are particular embodiments of the present invention.
    Type: Grant
    Filed: June 17, 1994
    Date of Patent: February 11, 1997
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Joseph R. Ecker, Joseph J. Kieber
  • Patent number: 5597717
    Abstract: Plant cells are transformed by:(i) transforming a plant cell whose growth is sensitive to inhibition by a sulfonamide or a salt thereof with a chimaeric gene comprising (a) a plant promoter, (b) a sulfonamide resistance gene having a sequence encoding a transmit peptide fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence;(ii) selecting a transformed plant cell whose growth is resistant to inhibition by a sulfonamide or salt thereof;(iii) optionally, regenerating from the transformed plant cell a genetically transformed plant which exhibit the said resistance;(iv) optionally, obtaining seed from the regenerated plant; and(v) optionally, propagating plants from the seed.The growth of weeds can be controlled at a locus where a transgenic plant obtained as above is being cultivated by applying to the locus an effective amount of a herbicide, such as asulam, which acts by inhibiting dihydroptercate synthase.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: January 28, 1997
    Assignee: Rhone-Poulenc Agrochimie Limited
    Inventors: Jean F. Guerineau, Philip M. Mullineaux, Edgar W. Parnell
  • Patent number: 5596131
    Abstract: The present invention provides cell cultures derived from graminaceous plants which cultures are capable of being regenerated into plants, including fertile plants. Methods of accomplishing this regeneration are also provided. In addition, a novel procedure for the cryopreservation of embryogenic cell cultures is described.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: January 21, 1997
    Assignee: Ciba-Geigy Corporation
    Inventors: Michael E. Horn, Christian T. Harms, Raymond D. Shillito
  • Patent number: 5596132
    Abstract: The invention described herein discloses a virus-induced resistance that may be transferred from one plant generation to another in which transgenic plants containing a coding sequence, taken from the read-through portion of the replicase portion of the viral genome, are resistant to subsequent disease by the virus. The use of the 54 kDa coding sequence from TMV is described as a specific example of the broader technology. Thus, the invention defines a means for bringing about viral resistance in plants which have been transformed with nucleic acid copies of fragments or segments taken from the replicase portion of the pathogenic virus genome. In addition, the present invention defines transformed plants and their seeds which carry a portion of the viral genome which codes for a portion of the read-through portion of the replicase genome of the pathogenic virus.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: January 21, 1997
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Milton Zaitlin, Daniel Golemboski, George Lomonossoff
  • Patent number: 5589623
    Abstract: A method for control of ethylene biosynthesis in plants comprising a vector containing a selective gene under plant promoter control, and a DNA insert comprising codons for a functional heterologous polypeptide having AdoMetase activity and flanked by a plant promoter on one side and a polyA signal sequence on the other side; and, transforming a plant host with said vector wherein the plant host transformed thereby is capable of expressing the heterologous polypeptide having AdoMetase activity under the control of said control region. The presence of the AdoMetase gene and the expression of AdoMetase in transgenic plants lowers AdoMet levels and generates an inhibitor of ACC synthase causing a corresponding decrease in ethylene biosynthesis and precursor availability.
    Type: Grant
    Filed: December 20, 1994
    Date of Patent: December 31, 1996
    Assignee: Agritope, Inc.
    Inventors: Adolph J. Ferro, Richard K. Bestwick, Lyle R. Brown
  • Patent number: 5589614
    Abstract: The chemically-inducible 27 kD subunit of the enzyme glutathione-S-transferase, isoform II (GST-II-27) and sequences encoding it are provided. In particular, a genomic DNA sequence encoding the gene promoter for the GST-II-27 subunit is provided. When linked to an exogenous gene and introduced into a plant by transformation, the GST-II-27 promoter provides a means for the external regulation of expression of that exogenous gene. Transformation with DNA encoding glutathione-S-transferase polypeptides produces herbicide resistance transgenic plants.
    Type: Grant
    Filed: May 23, 1994
    Date of Patent: December 31, 1996
    Assignee: Zeneca Limited
    Inventors: Ian G. Bridges, Simon W. J. Bright, Andrew J. Greenland, David C. Holt, Ian Jepson, Wolfgang W. Schuch