Patents Examined by Che S. Chereskin
  • Patent number: 5449767
    Abstract: Compounds having the structure: ##STR1## wherein B represents 7-deazapurine, or pyrimidine moiety covalently bonded to the C.sup.1 '-position of the sugar moiety, provided that when B is 7-deazapurine, it is attached at the N.sup.9 -position of the 7-deazapurine and when B is pyrimidine, it is attached at the N.sup.1 -position;wherein A represents a moiety consisting of at least three carbon atoms which is capable of forming a detectable complex with a polypeptide when the compound is incorporated into a double-stranded ribonucleic acid, deoxyribonucleic acid duplex, or DNA-RNA hybrid;wherein the dotted line represents a chemical linkage joining B and A, provided that if B is 7-deazapurine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine; andwherein each of x, y and z represents ##STR2## either directly, or when incorporated into oligo- and polynucleotides, provide probes which are widely useful.
    Type: Grant
    Filed: May 20, 1992
    Date of Patent: September 12, 1995
    Assignee: Yale University
    Inventors: David C. Ward, Pennina R. Langer, Alexander A. Waldrop, III
  • Patent number: 5447867
    Abstract: Recombinant DNA comprises promoter and terminator base sequences respectively upstream and downstream of a base sequence corresponding to a substantial sequence of bases in pectin esterase mRNA. The mRNA produced thereby delays softening of fruit, in particular tomatoes.
    Type: Grant
    Filed: February 26, 1993
    Date of Patent: September 5, 1995
    Assignee: Imperial Chemical Industries PLC
    Inventors: Ian Bridges, Wolfgang Schuch, Donald Grierson
  • Patent number: 5446019
    Abstract: The novel strain JHCC 5767 of Bacillus thuringiensis, and insecticidal compositions containing insecticidal protein(s) produced by said strain are provided. A process for protecting plants from insects of the genus Diabrotica by exposing larvae to insecticidal protein produced by said strain is also provided. Novel genes for insecticidal proteins may be isolated from strain JHCC 5767.
    Type: Grant
    Filed: January 6, 1994
    Date of Patent: August 29, 1995
    Assignee: Zeneca Limited
    Inventors: Susan Ely, Janet M. Tippett
  • Patent number: 5445961
    Abstract: This invention relates to the regeneration of fertile monocotyledonous plants from a plant composition that contains microspores. A method of the present invention comprises preculturing a plant composition under a plurality of stressful conditions that promote embryogenesis, isolating microspores at a temperature below about 25.degree. C., culturing the microspores in an embryoid/calli induction medium and regenerating a plant from an embryoid/calli. A solid, porous support system for transferring isolated microspores through a series of subcultures containing different media is also provided. The composition of the media are aspects of the invention.
    Type: Grant
    Filed: December 18, 1992
    Date of Patent: August 29, 1995
    Assignee: DeKalb Genetics Corporation
    Inventors: Anthony D. Genovesi, Richard A. Yingling
  • Patent number: 5446021
    Abstract: The present invention relates to an agricultural and horticultural composition inducing resistance in plants against salt- and water-stresses which comprises as an active ingredient an effective amount of at least one cyclic dipeptide of the following formula (I) or an agriculturally or horticulturally acceptable salt thereof; ##STR1## wherein each of R.sub.1 and R.sub.2, which may be the same or different, is a hydrogen atom or lower alkyl group, or R.sub.1 and R.sub.2 are joined to form pyrrolidine ring which may optionally have hydroxy group, and R.sub.3 is a hydrogen atom or hydroxy group.
    Type: Grant
    Filed: May 10, 1994
    Date of Patent: August 29, 1995
    Assignee: Nippon Zoki Pharmaceutical Co., Ltd.
    Inventors: Masaharu Kurohashi, Ko Nakamura, Kazuharu Ienaga
  • Patent number: 5441934
    Abstract: This invention provides a family of insecticidally effective peptides which may be isolated from Tegenaria spider venom, DNA encoding such insecticidally effective peptides and methods for controlling invertebrate pests.
    Type: Grant
    Filed: December 20, 1993
    Date of Patent: August 15, 1995
    Assignees: FMC Corporation, NPS Pharmaceuticals, Inc.
    Inventors: Karen J. Krapcho, John R. H. Jackson, Janice H. Johnson, Eric G. DelMar, Robert M. Kral, Jr.
  • Patent number: 5436002
    Abstract: Disclosed and claimed are toxins produced by novel Bacillus thuringiensis isolates designated B.t. PS92J, B.t. PS196S1, B.t. PS201L1, and B.t. PS201T6, which have dipteran and/or corn rootworm activity. Thus, the isolates, or mutants thereof, can be used to control such pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.
    Type: Grant
    Filed: September 30, 1993
    Date of Patent: July 25, 1995
    Assignee: Mycogen Corporation
    Inventors: Jewel Payne, Kenneth E. Narva, Kendrick A. Uyeda, Christine J. Stalder, Tracy E. Michaels
  • Patent number: 5436391
    Abstract: A synthetic gene encoding an insecticidal protein, the gene is provided having a base sequence which has been modified to bring the codon usage in conformity with the genes of gramineous plants particularly rice plants. A preferred gene sequence is shown in SEQ ID NO: 1. An expression vector containing said structural gene is provided. A method for producing recombinant gramineous plants is provided, which comprises transforming protoplasts derived from gramineous plants with the vector, incubating the protoplasts in a liquid medium in the presence of cultured cells of the plants to form colonies and regenerating plants from said colonies. Recombinant gramineous plants having resistance against insects are also provided.
    Type: Grant
    Filed: November 27, 1992
    Date of Patent: July 25, 1995
    Assignees: Mitsubishi Corporation, Mitsubishi Kasei Corporation
    Inventors: Hideya Fujimoto, Kimiko Ito, Mikihiro Yamamoto, Ko Shimamoto
  • Patent number: 5432273
    Abstract: A process for the preparation of 3'-substituted-2',3'-dideoxynucleosides is provided that utilizes inexpensive, non-carbohydrate, acyclic, achiral starting materials and that proceeds with high enantiomeric and stereochemical control. The method can be used to prepare the pharmaceutically important compounds 3'-azido-3'-deoxythymidine, 3'-azido-2',3'-dideoxyuridine, 3'-fluoro-3'-deoxythymidine, and 3'-fluoro-3'-deoxyuridine.
    Type: Grant
    Filed: October 14, 1992
    Date of Patent: July 11, 1995
    Assignee: Emory University
    Inventors: Dennis C. Liotta, Michael W. Hager
  • Patent number: 5430134
    Abstract: The present invention relates to a process for producing lipids containing the fatty acid petroselinic acid in plants. The production of petroselinic acid is accomplished by genetically transforming plants which do not normally accumulate petroselinic acid with a gene for a .omega.12 desaturase from another species which does normally accumulate petroselinic acid.
    Type: Grant
    Filed: August 7, 1992
    Date of Patent: July 4, 1995
    Assignee: Michigan State University
    Inventors: John B. Ohlrogge, Edgar B. Cahoon, John Shanklin, Christopher R. Somerville
  • Patent number: 5428001
    Abstract: Maize plants are grown in a planting area that are tolerant of inhibition by a cyclohexanedione herbicide, an aryloxyphenoxypropanoic acid herbicide, or mixtures thereof, at levels which normally inhibit growth and development, wherein said tolerance is conferred by an altered acetylcoenzyme A carboxylase (ACCase) tolerant of inhibition by the herbicide at levels which normally inhibit the activity of an unaltered ACCase. Additionally, an effective quantity of the named herbicide is applied so that the weeds (e.g., grass) are killed and the maize plants are uninjured. A representative cyclohexandione herbicide is sethoxydim and a representative aryloxyphenoxypropanoic acid herbicide is haloxyfop. Competition for the maize crop posed by weeds effectively is eliminated.
    Type: Grant
    Filed: January 11, 1994
    Date of Patent: June 27, 1995
    Assignee: Regents Of The University of Minnesota
    Inventors: David A. Somers, William B. Parker, Donald L. Wyse, John W. Gronwald, Burle G. Gengenbach
  • Patent number: 5426040
    Abstract: Methods for producing hybrid and transgenic strains of marine algae, and seaweeds and phycocolloids produced by the product seaweeds. Hybrid marine algae are produced by preparing protoplasts from spores of parental algal plants, fusing the spore-protoplast to form heterokaryon fusion products, isolating selected fusion products, and culturing these to produce multicellular material. Similarly, transgenic marine algae are produced by preparing protoplasts from spores of a marine algal plant, introducing foreign DNA into the resulting spore-protoplasts to form transformed spore-protoplasts, selecting certain transformed spore-protoplasts, and culturing these to produce transgenic multicellular material.
    Type: Grant
    Filed: September 23, 1993
    Date of Patent: June 20, 1995
    Assignee: Northeastern University
    Inventors: Donald P. Cheney, Clifford Duke
  • Patent number: 5422259
    Abstract: Procedure for the production of transgenic seedlings starting from genetically transformed buds, the said seedlings belonging to the species Cucumus melo and containing at least one gene introduced through the intermediary of Agrobacterium tumefaciens, characterized by the culture in two successive stages of genetically transformed buds, the first of these steps taking place in a plant cell culture medium containing a cytokinin and more particularly 6-benzyl aminopurine (BAP), and the second, which is performed when the buds have attained a height of about at least 3 mm, taking place in a plant cell culture medium containing as macro-elements:______________________________________ KH.sub.2 PO.sub.4 about 50 to about 100 mgL.sup.-1 MgSO.sub.4 about 75 to about 300 mgL.sup.-1 CaCl.sub.2.2H.sub.2 O about 500 to about 2500 mgL.sup.-1 KNO.sub.3 about 750 to about 1200 mgL.sup.-1 NH.sub.4 NO.sub.3 about 150 to about 200 mgL.sup.
    Type: Grant
    Filed: March 5, 1993
    Date of Patent: June 6, 1995
    Assignee: Biosem
    Inventors: Michiel De Both, Sophia Ben Tahar, Marianne Noel, Joel Perret
  • Patent number: 5416011
    Abstract: A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.
    Type: Grant
    Filed: November 23, 1993
    Date of Patent: May 16, 1995
    Assignee: Monsanto Company
    Inventors: Maud A. Hinchee, Dannette Connor-Ward
  • Patent number: 5416250
    Abstract: A method for control of ethylene biosynthesis in plants comprising a vector containing a selective gene under plant promoter control, and a DNA insert comprising codons for a functional heterologous polypeptide having AdoMetase activity and flanked by a plant promoter on one side and a polyA signal sequence on the other side; and,transforming a plant host with said vector wherein the plant host transformed thereby is capable of expressing the heterologous polypeptide having AdoMetase activity under the control of said control region. The presence of the AdoMetase gene and the expression of AdoMetase in transgenic plants lowers AdoMet levels and generates an inhibitor of ACC synthase causing a corresponding decrease in ethylene biosynthesis and precursor availability.
    Type: Grant
    Filed: June 8, 1994
    Date of Patent: May 16, 1995
    Assignee: Agritope, Inc.
    Inventors: Adolph J. Ferro, Richard K. Bestwick, Lyle R. Brown
  • Patent number: 5413937
    Abstract: Process for the inhibition of the production of a gene product in a plant cell which comprises generating in the cell while the gene product is being expressed mRNA from recombinant DNA coding for part only of the gene product: also constructs for use in the process, and cells and plants that carry out the process. Specifically applicable to control of fruit ripening, in particular in tomatoes.
    Type: Grant
    Filed: December 7, 1993
    Date of Patent: May 9, 1995
    Assignee: Zeneca Limited
    Inventors: Ian G. Bridges, Donald Grierson, Wolfgang Schuch
  • Patent number: 5403736
    Abstract: Formation of adventitious buds, adventitious embryos and plant bulblets are induced by mechanically making fine openings in plant tissue pieces or callus and introducing calmodulin and/or calcium ions into the plant tissue pieces or callus then culturing in an artificial culture medium.
    Type: Grant
    Filed: April 28, 1993
    Date of Patent: April 4, 1995
    Assignee: Mitsui Petrochemical Industries, Ltd.
    Inventor: Shizufumi Tanimoto
  • Patent number: 5391724
    Abstract: New genes for pinosylvine synthase ("pinosylvine synthase genes") have been found, which can be incorporated into the hereditary factors (the genome) of plants that generate no pinosylvine or only inadequate pinosylvine, whereby an increased resistance of these plants to pests can be brought about. Also disclosed are vectors, host organisms, and plants transformed with the new pinosylvine synthase genes.
    Type: Grant
    Filed: September 8, 1992
    Date of Patent: February 21, 1995
    Assignee: Bayer Aktiengesellschaft
    Inventors: Helmut Kindl, Rudiger Hain, Hans-Jorg Reif, Klaus Stenzel, Jurgen Thomzik
  • Patent number: 5387757
    Abstract: Fruit, especially tomatoes, having lowered expression of fruit-softening enzymes caused by antisense gene expression, and seeds therefrom.
    Type: Grant
    Filed: April 1, 1993
    Date of Patent: February 7, 1995
    Assignee: Zeneca Limited
    Inventors: Ian G. Bridges, Wolfgang W. Schuch, Donald Grierson
  • Patent number: 5380831
    Abstract: Synthetic Bacillus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.
    Type: Grant
    Filed: May 3, 1993
    Date of Patent: January 10, 1995
    Assignee: Mycogen Plant Science, Inc.
    Inventors: Michael J. Adang, Thomas A. Rocheleau, Donald J. Merlo, Elizabeth E. Murray