Patents Examined by Christian L. Fronda
  • Patent number: 11827917
    Abstract: Methods and systems for the enhancement of bioproduct process yields. The present invention provides systems and methods for improvement of the carbon conversion efficiency by yeast of an organic substrate such as a fermentable sugar to a fermentation product such as ethanol. Exemplary yield-increasing/growth-inhibitory compounds include tyrosol (TyrOH), 2-phenylethanol (PheOH), and tryptophol (TrpOH). Immobilization of the yeast, such as in a porous bead or pellet, can further increase yield. Exemplary immobilization included immobilization in calcium alginate beads. The system and methods taught herein demonstrate that product yield such as ethanol yield can be improved by adding yeast yield-increasing/growth-inhibitory molecules to reduce cell growth of yeast species, such as S. cerevisiae, suggesting a strategy to improve the yield of ethanol and other yeast fermentation products by manipulating native biological control systems.
    Type: Grant
    Filed: April 26, 2021
    Date of Patent: November 28, 2023
    Assignee: Colorado State University Research Foundation
    Inventors: Kenneth F. Reardon, Xingfeng Huang
  • Patent number: 11827912
    Abstract: The present invention relates to chimeric endolysin Lys109 which effectively controls Staphylococcus aureus, wherein the endolysin Lys109 of the present invention has a novel amino acid sequence that has not been conventionally studied. Endolysin Lys109 of the present invention can be used as a biological regulator capable of effectively inhibiting a wide range of Staphylococcus aureus and a biofilm produced thereby. In addition, endolysin Lys109 can kill Staphylococcus aureus without regard for resistance to conventional antibiotics, and thus can be widely used for treatment of diseases caused by Staphylococcus aureus infection. Moreover, endolysin Lys109 can also be used to resolve medical problems caused by Staphylococcus aureus which has antibiotic resistance.
    Type: Grant
    Filed: April 21, 2020
    Date of Patent: November 28, 2023
    Inventors: Sangryeol Ryu, Bokyung Son
  • Patent number: 11820737
    Abstract: Peroxyhydroxycarboxylic acid forming compositions and methods for forming peroxyhydroxycarboxylic acids, preferably in situ, using the peroxyhydroxycarboxylic acid forming compositions are disclosed. Methods of using the peroxyhydroxycarboxylic acids, including for treating a surface or a target in need of antimicrobial or sanitizing treatment are also disclosed. Particular applications of using odor-free, low volatility peroxyhydroxycarboxylic acid sanitizers for direct food contact are disclosed.
    Type: Grant
    Filed: January 29, 2021
    Date of Patent: November 21, 2023
    Assignee: Ecolab USA Inc.
    Inventors: Junzhong Li, Allison Prideaux, Richard Staub
  • Patent number: 11814660
    Abstract: The disclosure relates to recombinant microorganisms for the production of fatty amines and derivatives thereof. Further contemplated are cultured recombinant host cells as well as methods of producing fatty amines by employing these host cells.
    Type: Grant
    Filed: December 14, 2020
    Date of Patent: November 14, 2023
    Assignee: Genomatica, Inc.
    Inventors: Stephen B. Del Cardayre, Louis G. Hom
  • Patent number: 11807879
    Abstract: Blends of enzymes of the same enzyme class (same EC number), such as beta-glucuronidase enzyme blends, are provided that exhibit synergistic levels of activity across a range of substrates as compared to the activity levels of each enzyme in the blend individually. The blends also may exhibit a greater effective substrate range, as well as greater effective pH and/or temperature ranges as compared to single enzyme preparations. Predictive methods for determining optimal enzyme blends, methods for preparing enzyme blends, enzyme blend compositions, enzyme blend formulations and methods of using such enzyme blends are provided.
    Type: Grant
    Filed: December 10, 2021
    Date of Patent: November 7, 2023
    Inventors: John Tomashek, Caleb Reece Schlachter, Pongkwan Sitasuwan, Lim Andrew Lee
  • Patent number: 11807886
    Abstract: The present invention relates to processes for the formation of pyridinedicarboxylic acid (PDCA), in particular, 2,4-pyridinedicarboxylic acid (2,4-PDCA) and 2,5-pyridinedicarboxylic acid (2,5-PDCA), and mono- and diester derivatives thereof, from 3,4-dihydroxybenzoic acid, via a biocatalytic reaction using, for example, a protocatechuate dioxygenase such as protocatechuate 4,5-dioxygenase or protocatechuate 2,3-dioxygenase, and a nitrogen source. The invention also relates to copolymers that comprise the pyridinedicarboxylic acid monomers and derivatives thereof, processes for the formation of the copolymers and uses for the copolymers.
    Type: Grant
    Filed: March 10, 2020
    Date of Patent: November 7, 2023
    Inventors: Paul Law, Paul Mines, Timothy Bugg
  • Patent number: 11807880
    Abstract: The present invention discloses a recombinant modified extracellular chitinase having an amino acid sequence set forth in SEQ ID NO. 4 or SEQ ID NO. 5 prepared by substituting two tyrosine (Y) residues with histidine (H) in the native chitinase of Brevibacillus laterosporus LAK 1210. The modified chitinase represents an advancement as it has improved thermal stability, wider optimum pH range, high efficacy, improved solubility and low toxicity. The present invention also provides compositions and improved methods for producing and purifying the recombinant modified chitinase by chitin affinity chromatography and chitin adsorption affinity chromatography using shrimp shell and crab shell, for large scale production at low cost. The modified chitinase has wide range of applications including prevention, treatment or modulation of phytopathogenic infection in a plant or a plant part.
    Type: Grant
    Filed: September 17, 2021
    Date of Patent: November 7, 2023
    Assignee: Council of Scientific & Industrial Research
    Inventors: Lakshmi Prasanna Gangavaramu, Madhusudana Rao Nalam, Veerabhadra Rao Arravapalli
  • Patent number: 11807666
    Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.
    Type: Grant
    Filed: November 29, 2022
    Date of Patent: November 7, 2023
    Assignee: Cytiva BioProcess R&D AB
    Inventors: Martin Hall, Karolina Busson, Jean-Luc Maloisel, Helena Skoglar
  • Patent number: 11807889
    Abstract: Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding a phospholipase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
    Type: Grant
    Filed: October 7, 2019
    Date of Patent: November 7, 2023
    Assignee: Novozymes A/S
    Inventors: Monica Tassone, James Ron Huffman, Chee-Leong Soong, Jung Yi
  • Patent number: 11795484
    Abstract: The application discloses a method for preparing crude polysaccharides based on synergistic fermentation with corn stover and fungus, including the following steps: step (1): drying corn stover, pulverizing, placing in a hydrothermal reactor, adding deionized water, sealing the hydrothermal reactor, placing in a heating device, reacting, taking out reaction products, centrifuging and drying a solid; step (2): adding the products obtained in step (1) and dried bean curd residue to a container, adding distilled water, mixing uniformly, sterilizing to obtain a mixed material, inoculating a fungus inoculum under aseptic conditions, sealing the container and cultivating in the dark to obtain crude polysaccharides. The disclosure uses corn stover and bean curd residue as raw materials to achieve efficient utilization of discarded biomass, reduce environmental pollution, and improve utilization of corn stover.
    Type: Grant
    Filed: April 29, 2020
    Date of Patent: October 24, 2023
    Inventors: Yingjin Song, Guanyi Chen, Lei Zhong, Yingxiu Zhang, Fawei Lin, Caiyu Wang, Shuyan Meng
  • Patent number: 11795481
    Abstract: The present invention relates to a process for reducing and/or preventing an increase in lactic acid levels in a fermentation product production process, such as especially ethanol production, wherein a lytic polysaccharide monooxygenase (LPMO) or an enzyme composition comprising an LPMO is added before or during saccharification and/or fermentation, or before or during propagation, to reduce and/or prevent an increase in lactic acid levels.
    Type: Grant
    Filed: April 5, 2022
    Date of Patent: October 24, 2023
    Assignee: Novozymes A/S
    Inventors: Armindo Ribeiro Gaspar, Victor Gabriel Guadalupe Medina, Xin Li, Kelly Cristina Leite Mulder, Angela Shows
  • Patent number: 11788094
    Abstract: The present invention generally relates to the field of fermentation technology and microorganisms useful for such fermentations. The invention also relates to materials including nucleic acids and proteins useful for altering fermentation characteristics of microorganisms, and to microorganisms comprising such nucleic acids and/or proteins.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: October 17, 2023
    Assignee: BASF SE
    Inventors: Max Fabian Felle, Stefan Jenewein, Kerstin Hage, Holger Hartmann
  • Patent number: 11781126
    Abstract: Proteases are enzymes which hydrolyze protein enzymes, eliminating their activity. The present invention exploits the hydrolyzing activity of proteases including proteinase K, endoproteinase LysC and/or trypsin to control the activity of restriction enzymes and/or eliminate or reduce production of unwanted DNA or RNA fragments (known as star activity).
    Type: Grant
    Filed: November 12, 2022
    Date of Patent: October 10, 2023
    Assignee: ABclonal Science, Inc.
    Inventors: Zhenyu Zhu, Dapeng Sun, Aine Quimby, Michaela Shottes
  • Patent number: 11781118
    Abstract: The disclosure discloses preparation of an L-amino acid deaminase mutant and application thereof, belonging to the technical field of gene engineering. Through pmirLAAD protein modification, analysis of a flexible loop structure around a binding site of the pmirLAAD product, and design of the best mutant, the modification method of the disclosure overcomes the defect that the catalytic efficiency of the previous wild-type enzyme is reduced due to product inhibition, and is tested by experiments. Compared with the control, the catalytic efficiency (1.61 mM?1·min?1) and the product inhibition constant (5.4 mM) of the finally obtained best mutant pmirLAADM4 are respectively increased by 5.2 times and 5.7 times. The yield of ?-ketoisovaleric acid can reach 96.5 g/L, and the transformation rate is greater than 97%. By adopting the method of the disclosure, the cost can be greatly reduced, and the industrialization process of production of ?-ketoisovaleric acid by an enzymatic transformation method is accelerated.
    Type: Grant
    Filed: June 18, 2021
    Date of Patent: October 10, 2023
    Assignee: Jiangnan University
    Inventors: Jing Wu, Shanshan Pei, Jia Liu, Wei Song, Xiulai Chen, Qiuling Luo
  • Patent number: 11753658
    Abstract: A novel isolated Pichia stipitis strain is provided. The strain is capable of fermenting at least a pentose sugar in the presence of one or more inhibitory substances to produce ethanol. A method of utilizing the strain to produce ethanol is also provided.
    Type: Grant
    Filed: May 2, 2022
    Date of Patent: September 12, 2023
    Inventor: Erin Johnson
  • Patent number: 11746362
    Abstract: The present disclosure provides compositions and methods for rapid production of chemicals in genetically engineered microorganisms in a large scale. Also provided herein is a high-throughput metabolic engineering platform enabling the rapid optimization of microbial production strains. The platform, which bridges a gap between current in vivo and in vitro bio-production approaches, relies on dynamic minimization of the active metabolic network.
    Type: Grant
    Filed: January 14, 2022
    Date of Patent: September 5, 2023
    Inventors: Michael David Lynch, Zhixia Ye
  • Patent number: 11746344
    Abstract: A method for producing isoprene includes culturing E. coli, which has isoprene productivity and in which a gene encoding a recA protein is attenuated or deleted, in a medium containing a carbon source. Therefore, a great amount of isoprene may be produced within a short period of time, and thereby considerably decreasing isoprene production unit costs.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: September 5, 2023
    Inventors: Seon Won Kim, Jung Hun Kim, Myeong Seok Cha, Hui Jeong Jang
  • Patent number: 11746321
    Abstract: The invention provides engineered Vibrio sp. organisms that comprise a genetic modification to either or both of the lpxL and/or lpxM genes. The organisms score substantially lower in an in vitro endotoxin assay versus the unmodified or wild type organism. The organisms preserve substantially the growth rate of the corresponding unmodified organisms. The organisms can also have an exogenous nucleic acid cloned in the organism, or an exogenous nucleic acid encoding a protein, polypeptide, or peptide expressed by the organism, and optionally secreted from the organism.
    Type: Grant
    Filed: March 25, 2021
    Date of Patent: September 5, 2023
    Assignee: Telesis Bio Inc.
    Inventors: Matthew T Weinstock, Daniel G. Gibson, Daniel Strimling
  • Patent number: 11739307
    Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules ad cDNA and/or DNA libraries using modified reverse transcriptases.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: August 29, 2023
    Inventors: Arkadiusz Bibillo, Pranav Patel
  • Patent number: 11732282
    Abstract: The present invention concerns an efficient way to isolate L-fucose from a fermentation broth. The L-fucose contained in the fermentation broth is produced by microbial fermentation (bacterial or yeasts). The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide L-fucose in powder form, in granulated form as well as in form of L-fucose crystals.
    Type: Grant
    Filed: November 15, 2018
    Date of Patent: August 22, 2023
    Assignee: Chr. Hansen HMO GmbH
    Inventors: Markus Helfrich, Stefan Jennewein