Patents Examined by Christian L. Fronda
  • Patent number: 10808266
    Abstract: Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.
    Type: Grant
    Filed: June 17, 2019
    Date of Patent: October 20, 2020
    Assignee: S&P Ingredient Development, LLC
    Inventors: Paul Blum, Sambasiva Rao Chigurupati, Derrick Jermaine White
  • Patent number: 10801045
    Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.
    Type: Grant
    Filed: January 10, 2018
    Date of Patent: October 13, 2020
    Assignee: Ginkgo Bioworks, Inc.
    Inventors: Curt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
  • Patent number: 10801049
    Abstract: A method is provided for biosynthetic production of cannabinoids in microorganisms from a carbon source precursor. This method describes the genetic modifications needed to engineer microorganisms to produce cannabinoids as well as a method for identifying and quantifying cannabinoids from fermentation broth. A system is also provided for tuning the method to produce different cannabinoids of interest by systematically modulating the enzymes encoded by the genetic modifications introduced in the microorganism.
    Type: Grant
    Filed: June 14, 2019
    Date of Patent: October 13, 2020
    Assignee: SYNTIVA THERAPEUTICS, INC.
    Inventors: Adam Davidovich, Brian Chan, Neil Gupta, Alim Ladha
  • Patent number: 10793886
    Abstract: The invention is in the field of enzymology. More in particular, it provides a method for the isomerization of xylose into xylulose wherein the xylose is derived from lignocellulosic material.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: October 6, 2020
    Assignee: METGEN OY
    Inventors: Klara Birikh, Anu Minna Maaret Suonpää, Matti Wilhelm Heikkilä
  • Patent number: 10793839
    Abstract: The present invention relates to cell lines that are genetically modified to overexpress a ?-galactoside ?-2,3-sialyltransferase 1 (ST3Gal1), preferably human ST3Gal1, which can be used for the production of recombinant glycoproteins having highly or fully sialylated O-linked GalNAc glycans (GalNAc O-glycans), preferably core 1 GalNAc O-glycans, as well as to respective recombinant glycoproteins. Further, the present invention relates to respective methods of expressing recombinant glycoproteins, methods of increasing the degree of sialylation of recombinant glycoproteins, and methods of decreasing the micro-heterogeneity of GalNAc O-glycans. Finally, the present invention relates to respective uses of the above cell lines for the production of recombinant glycoproteins, for increasing the degree of sialylation of recombinant glycoproteins, and for decreasing the micro-heterogeneity of O-linked GalNAc glycans of recombinant glycoproteins.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: October 6, 2020
    Assignee: Cevec Pharmaceuticals GmbH
    Inventors: Silke Wissing, Jens Wölfel, Nicole Faust
  • Patent number: 10781398
    Abstract: The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.
    Type: Grant
    Filed: May 6, 2019
    Date of Patent: September 22, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Nathaniel Kreel, Joseph Jump
  • Patent number: 10774350
    Abstract: The present application relates to a method for fermentative production of oxidized coenzyme Q10 and high-content oxidized coenzyme Q10 prepared therefrom. For the method for fermentative production of oxidized coenzyme Q10, in a fermentation process of a production strain, the oxidation-reduction potential (ORP) of a fermentation broth is controlled to be ?50 to 300 Mv, and preferably the oxidation-reduction potential (ORP) of the fermentation broth is controlled to be 50 to 200 mV. By controlling the ORP of the fermentation broth, the method for fermentative production of oxidized coenzyme Q10 enables the oxidized coenzyme Q10 content in the coenzyme Q10 produced by microorganisms to reach 96% or more, and the product is substantially composed of a single component, which makes post-treatment more convenient.
    Type: Grant
    Filed: June 25, 2018
    Date of Patent: September 15, 2020
    Assignees: ZHEJIANG NHU COMPANY LTD., ZHEJIANG UNIVERSITY, HEILONGJIANG NHU BIOTECHNOLOGY COMPANY LTD., SHANGYU NHU BIOLOGICAL CHEMICAL CO., LTD.
    Inventors: Shenfeng Yuan, Hongwei Yu, Zhaofeng Chen, Yongqiang Zhu, Yi Min, Yong Li, Baishan Hu, Guisheng Qiu, Kai Yu
  • Patent number: 10767206
    Abstract: The invention provides a new fermentation process for producing gellan gum, which can control the feeding amount and feeding speed of the nitrogen sources accurately and quantitatively, and control the growth and gum production of the strains, so as to significantly improve the controllability, stability and yield of the fermentation process for producing gellan gum.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: September 8, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jie Zhao, Zhihui Gao, Wouter Adrianus Van Winden, Rogier Meulenberg
  • Patent number: 10767205
    Abstract: The present disclosure is directed to microorganisms that are genetically modified to express at least one exogenous enzyme involved in N-acetylglucosamine uptake and metabolism. Methods for the production of an exopolysaccharide are also disclosed. The exopolysaccharide comprises N-acetylglucosamine and D-glucose and may be used as a bioresorbable implant for soft tissue repair, replacement, or augmentation.
    Type: Grant
    Filed: March 6, 2018
    Date of Patent: September 8, 2020
    Assignee: DePuy Synthes Products, Inc.
    Inventor: Douglas D. Buechter
  • Patent number: 10760099
    Abstract: The present invention relates to the field of nanotechnology, more specifically to the manufacture or treatment of nanostructures, and in particular provides arsenic sulfide nanostructures, as well as a process for obtaining nanostructures of arsenic sulfide. The present invention provides a process for obtaining arsenic sulfide (As—S) nanostructures from a microorganism, which comprises the steps of culturing under appropriate conditions the strain Fusibacter ascotence in the presence of a source of sulfur and a source of arsenic; and recovering arsenic sulfide nanostructures (As—S) from the precipitate obtained from said culture. The present invention provides, also, a nanostructure of arsenic sulfide which is a nanowire having a monoclinic crystal structure. The present invention further provides a nanostructure of arsenic sulfide, which is a nanoparticle with a monoclinic crystal structure.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: September 1, 2020
    Assignee: UNIVERSIDAD CATÓLICA DEL NORTE
    Inventors: Cecilia Demergasso Semenzato, Lorena Escudero González, Antonio E. Serrano
  • Patent number: 10760062
    Abstract: Provided herein are methods and compositions for producing phenylpropanoid derivatives, such as chalcones and stilbenes, and dihydrophenylpropanoid derivatives, such as dihydrochalcones and dihydrostilbenes, in microorganisms. In particular, the disclosure provides recombinant microorganisms and methods of use thereof for producing phenylpropanoid derivative compounds and dihydrophenylpropanoid derivative compounds.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: September 1, 2020
    Assignee: EVOLVA SA
    Inventors: Michael Naesby, Ernesto Simón Vecilla, Michael Eichenberger, Beata Joanna Lehka, Walden Emil Bjørn-Yoshimoto, Niels Bjerg Jensen
  • Patent number: 10745675
    Abstract: Provided herein are compositions and methods for the synthesis of the trisaccharide, isomelezitose, using genetically modified glucansucrase enzymes from representative microorganisms, including lactic acid bacteria such as Leuconostoc mesenteroides. Various modified enzymes are detailed, increasing isomelezitose yields and provide the foundation for large-scale production of isomelezitose for food, industrial and biomedical applications.
    Type: Grant
    Filed: April 30, 2018
    Date of Patent: August 18, 2020
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventors: Gregory L. Cote, Christopher D. Skory
  • Patent number: 10745682
    Abstract: Ribulose-1,5-bisphosphate oxygenase (RuBisCO) protein fibers and a method of producing them are disclosed herein. The method of producing one or more RuBisCO protein fibers including obtaining RuBisCO, for example from tobacco, combining the RuBisCO with one or more plasticizers, heating the combination of the RuBisCO and the one or more plasticizers up to about 140 degrees C., filtering the heated combination through an about 20 ?m filter, and passing the filtered combination through an orifice to produce one or more RuBisCO protein fibers.
    Type: Grant
    Filed: June 14, 2017
    Date of Patent: August 18, 2020
    Assignee: R.J. Reynolds Tobacco Company
    Inventors: Andries Sebastian, John P. Mua, David Neil McClanahan, Sammy Eni Eni, Thaddeus J. Jackson
  • Patent number: 10745725
    Abstract: A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing a bacterium having an ability to produce an L-amino acid, which has been modified so that the activity of a non-PTS fructose-uptake carrier and the activity of fructokinase are both increased, in a medium containing fructose, and collecting the L-amino acid from the medium or cells of the bacterium.
    Type: Grant
    Filed: March 27, 2018
    Date of Patent: August 18, 2020
    Assignee: AJINOMOTO CO., INC.
    Inventors: Kosuke Yokota, Kazuyuki Hayashi
  • Patent number: 10731104
    Abstract: The present invention relates to processes of recovering oil after liquefaction and/or from thin stillage and/or syrup/evaporated centrate from a fermentation product production process by adding a thermostable protease to the whole stillage, thin stillage and/or syrup.
    Type: Grant
    Filed: April 18, 2018
    Date of Patent: August 4, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Nathaniel Kreel, Joseph Jump
  • Patent number: 10731195
    Abstract: The present invention relates generally to the production of steviol glycosides. Provided is a method for enzymatically providing a modified steviol glycoside, comprising incubating a steviol glycoside substrate in the presence of sucrose and the glucansucrase GTF180 of Lactobacillus reuteri strain 180, or a mutant thereof having the desired transglycosylation activity. Also provided are modified steviol glycosides obtainable by a method of the invention, and the use thereof as low-glycemic sweetener.
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: August 4, 2020
    Assignee: Rijksuniversiteit Groningen
    Inventors: Evelien Maria te Poele, Lubbert Dijkhuizen, Gerrit Johannes Gerwig, Johannis Paulus Kamerling
  • Patent number: 10731138
    Abstract: The present invention discloses a formate dehydrogenase mutant with improved enzyme activity and stability and a construction method thereof, which belongs to the technical field of genetic engineering. The mutant of the present invention is obtained by mutating alanine at a 10th site to cysteine based on the amino acid shown in SEQ ID NO. 2. The specific enzyme activity of the mutant enzyme obtained by the present invention is improved by 1.3 times compared with that before the mutation, a half-life period (t1/2) at 60° C. is increased by 6.8 times compared with that in the mutation period, the copper ion tolerance is increased by 30 times compared with that before the mutation, and when pH is 4, the stability is improved by 2.0 times, and the catalytic efficiency is increased by 1.4 times.
    Type: Grant
    Filed: November 14, 2016
    Date of Patent: August 4, 2020
    Assignee: Jiangnan University
    Inventors: Zhiming Rao, Junxian Zheng, Taowei Tao, Junping Zhou, Xian Zhang, Meijuan Xu
  • Patent number: 10730909
    Abstract: A method for expressing, as a soluble protein or an active-form mutant enzyme, an enzyme that cannot be expressed as a soluble protein or an active-form enzyme in a heterologous expression system or that is obtained in a minute amount even when an active-form enzyme is expressed, the method including a technique for selecting an effective mutation site and a mutated amino acid. A new active-form mutant enzyme is also disclosed. The method involves: specifying an insoluble protein or an inactive-form enzyme; specifying a hydrophilic amino acid in a hydrophobic domain and/or a hydrophobic amino acid in a hydrophilic domain of an ?-helix structure portion of the insoluble protein or the inactive-form enzyme and preparing a gene that codes for an amino acid sequence in which a substitution is made to the hydrophilic amino acid in the hydrophobic domain and/or the hydrophobic amino acid in the hydrophilic domain.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: August 4, 2020
    Assignee: Toyama Prefectual University
    Inventors: Yasuhisa Asano, Daisuke Matsui, Yuko Oku
  • Patent number: 10724023
    Abstract: The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having a S. cerevisiae mating factor alpha 2 (Sc MF?2) or repressible acid phosphatase (Sc PHO5) secretion signal.
    Type: Grant
    Filed: July 15, 2019
    Date of Patent: July 28, 2020
    Assignee: CARGILL, INCORPORATED
    Inventors: Christopher K. Miller, Ana Negrete-Raymond, Jonathan Dwight Veldhouse, Amit Vas
  • Patent number: 10724056
    Abstract: A novel isolated Pichia stipitis strain is provided. The strain is capable of fermenting at least a pentose sugar in the presence of one or more inhibitory substances to produce ethanol. A method of utilizing the strain to produce ethanol is also provided.
    Type: Grant
    Filed: July 24, 2019
    Date of Patent: July 28, 2020
    Assignee: Province Brands
    Inventor: Erin Johnson