Patents Examined by Christopher L. Chin
  • Patent number: 10302536
    Abstract: Systems and devices for the isolation of biological agents that are infused in a solution causing those agents to float. These systems and devices have numerous research, diagnostic, clinical, consumer, and other applications. An aspect of the disclosure provides for an apparatus for isolation or enrichment of biological agents from a sample comprising: a sample container comprising an inner volume to hold a sample and a plurality of micro bubbles, a tapered end and an open end, a plunger inserted into the open end and a tip of the tapered end forming an acute angle relative to the horizontal axis of the sample container, wherein the plurality of micro bubbles float one or more biological agents of the sample and wherein the plunger moves towards the tip to isolate or enrich the one or more biological agents from the sample.
    Type: Grant
    Filed: May 8, 2015
    Date of Patent: May 28, 2019
    Assignee: DIAGNOLOGIX, LLC
    Inventors: Guixin Shi, Michael Benchimol, Tyler Watson
  • Patent number: 10302637
    Abstract: A device for detecting target biomolecules is provided. The device consists of an electrically conductive membrane having a biological recognition component configured to bind a target biomolecule immobilized thereon. The membrane is connected to an electric circuit by means of electrodes. A voltage source applies a voltage to the membrane and a resistance monitoring device monitors the resistance of the membrane as a selected volume of fluid sample suspected of containing the target biomolecule is delivered onto the membrane.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: May 28, 2019
    Assignee: Stellenbosch University
    Inventors: Christiaan Gunter Alwyn Viviers, Willem Jacobus Perold, Leon Milner Theodore Dicks, Giles Hubert Coyle Maybery
  • Patent number: 10267791
    Abstract: A biological test strip with isolation structure includes a substrate having a testing portion and a measuring portion; an electrode disposed on the substrate and including an operation electrode and an auxiliary electrode, the operation electrode having a first operating end and a first reading end, the auxiliary electrode having a second operating end and a second reading end; a first flow confining member surrounding the first operating end; and a second flow confining member surrounding the first flow confining member and the second operating end, with a height of the second flow confining member larger than a height of the first flow confining member. Therefore, the flowing scope of the dropped liquid is confined, so as to fix the square measure of the dropped liquid contacting the electrode and thereby improve the analysis repeatability.
    Type: Grant
    Filed: May 4, 2017
    Date of Patent: April 23, 2019
    Inventors: Wen-Chih Wang, Bing-Chen Gu, Yu-Han Dai
  • Patent number: 10255478
    Abstract: The invention relates generally to both a method and apparatus for the creation of full resolution color digital images of diagnostic cassettes or objects of interest using a gray-scale digital camera or sensor combined with time sequential illumination using additive primary colors followed by post exposure digital processing. Such procedures and equipment is of significant economic value when employed in situations such as diagnostic clinical analyzers where space is limited and image quality requirements are high.
    Type: Grant
    Filed: June 4, 2015
    Date of Patent: April 9, 2019
    Assignee: Ortho-Clinical Diagnostics, Inc.
    Inventors: Jens H. Jorgensen, Michael W. LaCourt, Donald J. Moran, Jr., David J. Hawks, Joseph Wycallis, Stephen C. Arnold
  • Patent number: 10241311
    Abstract: The invention relates to a method for observing a sample under optical microscopy, in incoherent, unpolarised light, using a sample substrate including a contrast-amplifying layer having a complex index of refraction. The invention also relates to a method for detecting or metering at least one chemical or biological species using such a sample substrate.
    Type: Grant
    Filed: July 26, 2013
    Date of Patent: March 26, 2019
    Assignees: Centre National de la Recherche Scientifique, Universite du Maine, Universite d'Aix-Marseille
    Inventors: Dominique Ausserré, Ludovic Roussille, Myriam Zerrad, Fabien Lemarchand, Claude Amra
  • Patent number: 10241110
    Abstract: Plasmonic nanotransducers, methods of preparing plasmonic nanotransducers, and methods for label-free detection of target molecules are disclosed. The plasmonic nanotransducers include hollow nanostructure cores and artificial antibodies. The plasmonic nanotransducers are exposed to a biological sample that can contain the specific target molecules. The plasmonic nanotransducers can be analyzed with surface enhanced Raman scattering techniques and/or localized surface plasmon resonance techniques to quantify the amount of the target molecule in the sample.
    Type: Grant
    Filed: October 17, 2014
    Date of Patent: March 26, 2019
    Assignee: Washington University in St. Louis
    Inventors: Srikanth Singamaneni, Limei Tian, Keng-Ku Liu, Abdennour Abbas, Jeremiah J. Morrissey, Evan D. Kharasch
  • Patent number: 10232365
    Abstract: A test device (80) for testing for the presence of a substance in a sample held on a sample carrier (30) includes a holder (82) for holding a sample carrier (30) which includes one or more chambers (50,52). The test device (80) also includes an actuator for compressing or depressurizing the chamber(s) (50,52). The actuator is configured to prevent reinflation of the chamber(s) (50,52) after compression or depressurization.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: March 19, 2019
    Inventors: Michael Chard, Robert Porter, Courtney Nicholson, Andrew Lakey, Simon Attree, Philip Walsh, Philip Potter, Craig Wightman, Paul Dyckes, Holger Becker, Rene Stewart, Wolfgang Engelhart, Claudia Gaertner
  • Patent number: 10234390
    Abstract: Provided herein is an optical biosensor for detecting a target bioanalyte in a sample. The biosensor includes: a porous silicon or alumina substrate having a surface and a detection agent immobilised on the surface. The detection agent includes a sensing domain and a signaling domain, the sensing domain having a linker capable of interacting with the target bioanalyte and the signaling domain having a luminescence donor and a luminescence acceptor wherein the luminescence donor and the luminescence acceptor are connected by the linker and are optically coupled in the absence of the target bioanalyte. Emission of light from the luminescence donor is substantially quenched by the luminescence acceptor, and interaction of the target bioanalyte with the linker results in optical un-coupling of the luminescence donor and the luminescence acceptor to thereby result in light emission from the luminescence donor.
    Type: Grant
    Filed: March 3, 2015
    Date of Patent: March 19, 2019
    Inventor: Nicolas H. Voelcker
  • Patent number: 10209249
    Abstract: Labelled silica nanoparticles for immunochromatographic reagent, comprising silica nanoparticles containing a labelled substance.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: February 19, 2019
    Inventors: Hideki Aizawa, Michio Ohkubo
  • Patent number: 10166540
    Abstract: A nanofluidic biosensor system (200) comprising a bottom substrate (120) and a top substrate (110) between which are defined an input lateral aperture (210), a nanoslit (230) which contains at least one functionalized area (231) and an output lateral aperture (220) or an internal reservoir (221), said biosensor system (200) being adapted to let a solution containing biomolecules (320) enter the input lateral aperture (210) and successively pass through said nanoslit (230) and said output lateral aperture (220) or internal reservoir (221); said biosensor system (200) furthermore comprising a gas evacuation subsystem (150-155) which is located between said nanoslit (230) and the biosensor external environment.
    Type: Grant
    Filed: December 17, 2014
    Date of Patent: January 1, 2019
    Assignee: Abionic SA
    Inventors: Nicolas Durand, Iwan Maerki, Matthias Geissbuehler
  • Patent number: 10161934
    Abstract: Biosensor components (chips) are described based on direct biocoating processes that result in the tenacious and stable, noncovalent (believed to be chemisorptive) binding of anchor substances such as avidin(s) other proteins having specific binding partners or oligo- or poly-nucleotides onto any piezo-electrically active crystal surface. The resulting platform technology can be developed for a variety of biosensors with specific applications in biological assays. The table mono layers of the anchor substances forms reactive layers, ready to bind a capture reagent such as a biot-inylated antibody for capture and detection of analytes in biologic fluid samples.
    Type: Grant
    Filed: August 27, 2012
    Date of Patent: December 25, 2018
    Assignee: Aviana Molecular Technologies, LLC
    Inventors: Lisa Laury-Kleintop, Herman Rutner
  • Patent number: 10124337
    Abstract: Various embodiments are described herein for controlling the self-assembly process of paramagnetic particles and the final particle cluster size using a liquid-liquid interface. The number of paramagnetic particles within a particle cluster and coating at the liquid-liquid interface may be controlled by systematically varying the strength of an applied magnetic field gradient and the interfacial tension of the liquid-liquid interface.
    Type: Grant
    Filed: February 3, 2017
    Date of Patent: November 13, 2018
    Inventors: Scott Tsai, Steven Jones, Eric Jervis
  • Patent number: 10119962
    Abstract: A recessed portion and a protruding portion arranged periodically are formed on a base portion. In the recessed portion, an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes. The width of the protruding portion is smaller than the average particle diameter of the exosomes.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: November 6, 2018
    Inventors: Makoto Itonaga, Shingo Yagyu, Yuichi Hasegawa, Koji Tsujita, Masayuki Ono
  • Patent number: 10107807
    Abstract: Techniques for enhanced fluorescence include a functionalized substrate for a target optical frequency comprising a one dimensional photonic crystal that is functionalized with a bioactive target molecule that has an affinity for a particular analytic. The one dimensional photonic crystal includes a plurality of dielectric layers including a plurality of high index of refraction layers alternating with a plurality of low index of refraction layers. The thickness of each layer is within a factor of four of a wavelength of the optical frequency in the layer. For emissions from a fluorophore bound to the target molecule and excited by incident light, there is an emission intensity maximum centered at an angle independent of the direction of the incident light.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: October 23, 2018
    Assignee: The University of Maryland, Baltimore
    Inventors: Joseph R. Lakowicz, Ramachandram Badugu
  • Patent number: 10088478
    Abstract: Disclosed are nanostructured gold films which may be produced by solution-phase depositions of gold ions onto a variety of surfaces. The resulting plasmonic gold films are used for enhanced spectroscopic-based immunoassays in multiplexed microarray format with detection mechanisms based on either surface-enhanced Raman scattering or near-infrared fluorescence enhancement. The preparation of the films and subsequent modifications of the gold film surfaces afford increased sensitivity for various microarrays. The films are discontinuous, forming gold “islands.” Sensitivity, size, shape, and density of the nanoscopic gold islands comprising the discontinuous nanostructured gold film are controlled to enhance the intensity of Raman scattering and fluorescence in the near-infrared, allowing for improved measurements in clinical diagnostic or biomedical research applications.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: October 2, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Hongjie Dai, Scott M. Tabakman, Guosong Hong, Bo Zhang
  • Patent number: 9410949
    Abstract: Natural and/or synthetic antibodies for specific proteins are adhered to nanoparticles. The nanoparticles are adhered to a substrate and the substrate is exposed to a sample that may contain the specific proteins. The substrates are then tested with surface enhanced Raman scattering techniques and/or localized surface plasmon resonance techniques to quantify the amount of the specific protein in the sample.
    Type: Grant
    Filed: December 2, 2011
    Date of Patent: August 9, 2016
    Assignee: Washington University in St. Louis
    Inventors: Srikanth Singamaneni, Evan Kharasch, Jeremiah J. Morrissey, Chang Hee Lee
  • Patent number: 8067159
    Abstract: A microfluidic device comprising a first surface portion and a first sample retaining element, which have differing affinities to a fluid, and a method comprising supplying a sample to such a device. In some embodiments, the differing affinity is a result of plasma, ion embedding, surface charging, chemical, optical, electronic and/or electromagnetic treatment. Also, a microfluidic device comprising at least one microcapillary device having a sample retaining element, at least one surface of which exhibits hydrophobicity, hydrophilicity, electromagnetic force exertion and electrostatic force exertion. Also, a microfluidic device comprising a first element having a hydrophilic pattern comprising at least a first sample retaining element. Also, a method comprising supplying a sample to a channel between a first element and a second element, and inducing in the first element at least one hydrophilic pattern by electrets or by internal or external electrodes to provide a charged surface.
    Type: Grant
    Filed: August 13, 2007
    Date of Patent: November 29, 2011
    Assignees: Applied Biosystems, LLC, The United States of America as represented by the Department of Health and Human Services
    Inventors: James F. Brown, Jonathan E. Silver
  • Patent number: 7972778
    Abstract: A miniaturized assembly is provided whereby a fluid sample can be divided into a plurality of sample portions in retaining wells and the sample fluid can be displaced from open ends of the wells while simultaneously being sealed in the wells. A method of dividing a fluid sample using the assembly is also provided.
    Type: Grant
    Filed: March 11, 2004
    Date of Patent: July 5, 2011
    Assignees: Applied Biosystems, LLC, The United States of America as represented by the Department of Health and Human Services
    Inventors: James F. Brown, Jonathan E. Silver
  • Patent number: 7947490
    Abstract: The invention herein described relates to an assessment device comprising an assay part and a detachable recording part. The assessment device facilitates the rapid assaying and processing of tissue/fluid samples by healthcare workers. Also, advantageously, the result of the assay is only apparent to the healthcare worker after interrogation of the recording part at a processing facility.
    Type: Grant
    Filed: February 13, 2008
    Date of Patent: May 24, 2011
    Assignee: SmartSensor Telemed Ltd.
    Inventor: James Richard Jackson
  • Patent number: RE43979
    Abstract: The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.
    Type: Grant
    Filed: February 5, 2003
    Date of Patent: February 5, 2013
    Assignee: Abbott Laboratories
    Inventors: Oystein Fodstad, Gunnar Kvalheim