Patents Examined by Christopher L. Chin
  • Patent number: 11828755
    Abstract: In various embodiments methods and devices are provided for the detection and/or quantification of an analyte. In certain embodiments a device is provided comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase where, in use, said first phase solution becomes a leading phase and said second phase solution becomes a lagging phase; a lateral-flow assay (LFA); and a probe and/or a development reagent, where in use, said probe associates with said first phase solution in said leading phase of said ATPS and/or said development reagent associates with said second phase solution in said lagging phase of said ATPS. In certain embodiments a “one-pot” system of purifying and amplifying a nucleic acid is provided utilizing, e.g., an ATPS and isothermal amplification reagents.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: November 28, 2023
    Assignee: The Regents of the University of California
    Inventors: Daniel Takashi Kamei, Benjamin Ming Wu, Daniel William Bradbury, Shin Ting Sherine Frieda Cheung
  • Patent number: 11815511
    Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.
    Type: Grant
    Filed: November 16, 2020
    Date of Patent: November 14, 2023
    Inventors: Qi Zhang, Xiaoqian Tang, Yizhen Bai, Wen Zhang, Peiwu Li
  • Patent number: 11808763
    Abstract: A method of making a plasmon-resonance biosensor includes conjugating precious metal nanorods with form factor at least 1.5 with a biological probe material. Microfluidic chambers of volume under 0.025 microliter are formed over a substrate, and an aqueous suspension of the conjugated nanorods is injected into the chambers. The nanorods are organized in rows and aligned in long dimension along the rows. The biosensor is configured to be read by obtaining an optical absorption spectrum upon exposure to the analyte. The biosensor includes precious metal nanorods organized in rows with long dimension approximately parallel to the rows. The nanorods are conjugated with biological probes capable of binding to an analyte, the probes may be an aptamer, an antibody, a protein-nucleic acid (PNA), a complimentary DNA, or an enzyme having a binding site.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: November 7, 2023
    Inventors: Amogha Tadimety, John X. J. Zhang
  • Patent number: 11802868
    Abstract: A method for providing variable function medical tests, comprising providing by a mobile device application a plurality of selectable medical test functions, receiving information from the mobile device application regarding test results from a test performed using a testing device, wherein the testing device includes an alignment target disposed on the testing device and a plurality of immunoassay test strips receiving at the server an image of the testing device from the mobile device application, determining by the server RGB values for a plurality of pixels of the image, normalizing by the server the RGB values into a single value, comparing by the server the single value to a control value stored on the server, and providing by the server a risk indicator, wherein the risk indicator indicates a likelihood of a presence of a medical condition.
    Type: Grant
    Filed: November 6, 2017
    Date of Patent: October 31, 2023
    Inventors: Jovan Hutton Pulitzer, Henry Joseph Legere, III
  • Patent number: 11802871
    Abstract: An immunofixation electrophoresis applicator system and method deposits antisera in spaced apart locations on a substrate where the antisera is deposited as an elongated bead in a first direction and retained against migration in a direction transverse to said first direction at least by surface tension.
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: October 31, 2023
    Inventors: James Matthew Simmons, Jeffrey Allen Spencer, Charles David Kelley, Gaston Andres Del Pino
  • Patent number: 11768155
    Abstract: The present disclosure provides a detection device capable of detecting a low concentration of an analyte with high sensitivity. The detection apparatus according to the present disclosure comprises a metal microstructure on which a first VHH antibody having a property of binding specifically to the analyte is immobilized and which generate surface plasmon by being irradiated with excitation light, an inlet through which a second VHH antibody and a sample that may contain an analyte are introduced, wherein the second VHH antibody has a property of binding specifically to the analyte and is labeled with a fluorescent substance, a light source for irradiating the metal microstructure to which the second VHH antibody and the sample have been introduced with the excitation light, and a detection unit for detecting the analyte on the basis of fluorescence generated from the fluorescent substance by the irradiation of the excitation light.
    Type: Grant
    Filed: January 20, 2020
    Date of Patent: September 26, 2023
    Inventors: Hiroto Yanagawa, Masahiko Shioi, Kazuaki Nishio, Emina Ikeuchi
  • Patent number: 11761958
    Abstract: Devices, systems, and methods are provided for magnetic focus enhanced lateral flow assays. The devices and systems are ultrasensitive and provide for the visual detection of the presence or absence of one or more target analytes—which may include pathogens, proteins, or even molecules smaller than the foregoing—even when such analytes are only present in very limited amounts. The devices and systems include an immunostrip with a magnet positioned adjacent thereto, and magnetic probes specific to a target analyte that bind to the target analyte with specificity if present within a fluid sample to be tested. Methods are also provided for detecting one or more target analytes using magnetic focus, such methods including a step of controlling movement of a target analyte complex on an immunostrip incorporating a magnetic field, where such control slows a flow of the target analyte complex through a capture area on the immunostrip.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: September 19, 2023
    Assignee: Purdue Research Foundation
    Inventors: Joseph M K Irudayaraj, Wen Ren
  • Patent number: 11754554
    Abstract: The present invention provides fluidic devices and systems that allow detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.
    Type: Grant
    Filed: January 11, 2017
    Date of Patent: September 12, 2023
    Assignee: Labrador Diagnostics LLC
    Inventors: Shaunak Roy, Melissa Takahashi, Ian Gibbons, Edmond Ku, Thanh Dang, Tammy Burd, Adam Vollmer, Elizabeth A. Holmes
  • Patent number: 11747279
    Abstract: An optical biomodule for detecting a disease specific biomarker, utilizing an enhanced light emission due to integration of one or more three-dimensional (3-D) protruded structures in a fluidic container (which includes one or more materials), upon chemical binding of a disease specific biomarker binder (which includes a first segment coupled with a first fluorophore and a second segment coupled with a second fluorophore) with its corresponding disease specific biomarker is disclosed. Furthermore, the disease specific biomarker binder may include a synthetic biological logic circuit/gate. Furthermore, the three-dimensional (3-D) protruded structure can be coupled with a photonic crystal or a metamaterial (hyperbolic metamaterial/metamaterial of Epsilon-Near-Zero (ENZ)) of a suitable wavelength range.
    Type: Grant
    Filed: January 6, 2020
    Date of Patent: September 5, 2023
    Inventor: Mohammad A. Mazed
  • Patent number: 11740202
    Abstract: The presence of biomarkers or other analytes can be detected in the bodily fluid using Electrochemical Impedance Spectroscopy (EIS) or Electrochemical Capacitance Spectroscopy (ECS) in devices, such as handheld point-of-care devices. The devices, as well as systems and methods, utilize using Electrochemical Impedance Spectroscopy (EIS) or Electrochemical Capacitance Spectroscopy (EIS) in combination with an antibody or other target-capturing molecule on a working electrode. Imaginary impedance or phase shift, as well as background subtraction, also may be utilized.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: August 29, 2023
    Inventors: Andrew Douglas Penman, Marcus Willard Smith, Catalina Valencia, Sergei A Svarovsky
  • Patent number: 11740205
    Abstract: Sensors, as well as systems and methods of using the same are provided. Aspects of the sensors include a piezoelectric base, a plurality of surface-associated compositions that are stably associated with the piezoelectric base, and a plurality of crosslinking compositions that are configured to crosslink one or more surface-associated compositions in the presence of an analyte. The sensors, systems and methods described herein find use in a variety of applications, including the detection of an analyte in a sample.
    Type: Grant
    Filed: April 15, 2020
    Date of Patent: August 29, 2023
    Inventors: David H. Sierra, Andrew Korey
  • Patent number: 11719707
    Abstract: The present invention relates to methods of detecting CD200L in a female subject from a secretory phase biological sample. Increased levels of CD200L have been determined to be associated with endometriosis. CD200L is therefore a useful biomarker for the diagnosis of endometriosis and a useful target for therapeutic intervention.
    Type: Grant
    Filed: April 30, 2020
    Date of Patent: August 8, 2023
    Assignee: McMaster University
    Inventors: David Alexander Clark, Warren George Foster
  • Patent number: 11719643
    Abstract: A method for detecting dust mite antigens includes the steps of collecting a dust sample, applying an extraction and cleanup procedure for dust mite antigens from the dust sample in order to obtain a sample solution ready for measurement, and placing the sample solution on a SERS chip without immunological modification and under a Raman spectrometer for SERS detection in order to identify whether any dust mite antigens exist in the sample solution.
    Type: Grant
    Filed: May 24, 2019
    Date of Patent: August 8, 2023
    Inventors: Chun-Yu Chuang, Pin-Hsuan Yeh, Chao-Ming Tsen, Ching-Wei Yu, Wei-Chung Chao, Yung-Hsiang Wang, Cheng-Chien Li
  • Patent number: 11719624
    Abstract: The present invention relates to a liquid immersion micro-channel measurement device and measurement method which are based on trapezoidal incident structure prism incident-type silicon, and according to one embodiment of the present invention, the liquid immersion micro-channel measurement device based on trapezoidal incident structure prism incident-type silicon comprises: a micro-channel structure including a support and at least one micro-channel, which is formed on the support and has a sample detection layer to which a first bioadhesive material for detecting a first sample is fixed; a quadrangular pyramid-shaped prism formed on the upper part of the micro-channel structure; a sample injection unit for injecting, into the micro-channel, a buffer solution containing the first sample; a polarized light generation unit for emitting incident light polarized through the prism on the micro-channel at an incident angle that satisfies a p-wave non-reflection condition; and a polarized light detection unit for d
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: August 8, 2023
    Inventors: Hyun Mo Cho, Yong Jai Cho, Won Chegal
  • Patent number: 11703513
    Abstract: A system for determining a concentration of hemoglobin A1C includes a first electrochemical test strip, the first electrochemical test strip providing for an HbA1C concentration; and a second electrochemical test strip, the second electrochemical test strip providing for the total amount of hemoglobin.
    Type: Grant
    Filed: August 7, 2017
    Date of Patent: July 18, 2023
    Assignee: Polymer Technology Systems, Inc.
    Inventors: Gary Hughes, Ryan Jesswein, Brittney Werner, Aniruddha Patwardhan
  • Patent number: 11649263
    Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.
    Type: Grant
    Filed: May 28, 2019
    Date of Patent: May 16, 2023
    Assignee: ProteinSimple
    Inventors: Hui Xu, Kenneth Swartz
  • Patent number: 11639926
    Abstract: A detection device, the device comprising a sample liquid bearing device (300), test strips (200), a test strips bearing plate (100) and a signal acquisition positioning assisting device, wherein the sample liquid bearing device (300) comprises two or more samples cells (320) used for containing sample liquid, each of the test strips (200) at least comprises an identity information bearing part (250), a developing part (220) and a capillary part (210), the test strip bearing plate (100) is used for placing the test strips (200) in the same circular ring in a circumferential array radial manner, and the signal acquisition positioning assisting device is arranged on the test strip bearing plate and is used for positioning the test strips.
    Type: Grant
    Filed: May 13, 2022
    Date of Patent: May 2, 2023
    Assignee: Institute of Quality Standard and Testing Technology for Agro-Products, CAAS
    Inventors: Jing Wang, Zhen Cao, Hui Li
  • Patent number: 11619628
    Abstract: The present disclosure relates to a device including a reagent portion in which a chemiluminescent indicator and a chemiluminescent substrate for the indicator are disposed, and a base on which the reagent portion is formed. The chemiluminescent indicator and the chemiluminescent substrate are disposed independently from each other in the reagent portion in such a manner that the chemiluminescent indicator and the chemiluminescent substrate can react with each other when a sample is supplied to the reagent portion. The present disclosure also relates to a remote diagnosis system including an imaging terminal for detecting a luminescent signal generated when a reagent is supplied to the device and an information processing unit for processing luminescent signal data obtained by the imaging terminal. The imaging terminal and the information processing unit can bi-directionally communicate with each other via a network.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: April 4, 2023
    Assignee: Osaka University
    Inventors: Takeharu Nagai, Yoshiyuki Arai, Megumi Iwano
  • Patent number: 11619584
    Abstract: A time-resolved fluorescence immunochromatographic test paper card for testing clothianidin. A binding pad includes a detection microsphere and a quality control microsphere thereon, wherein the detection microsphere is a fluorescent microsphere coated with a clothianidin monoclonal antibody on the surface thereof, the quality control microsphere is a fluorescent microsphere coated with a rabbit anti-tag protein on the surface thereof. An NC film is provided with a detection line and a control line. The lengths of the detection line and the control line are the same as the width of the NC film. A first clamping part is arranged at one end of the sample pad. A second clamping part and a third clamping part are respectively arranged at two ends of the binding pad. A fourth clamping part is arranged at one end of the NC film, the other end of the NC film is joined with an absorption pad.
    Type: Grant
    Filed: July 9, 2020
    Date of Patent: April 4, 2023
    Inventors: Xiuguo Wang, Xiao Zhang, Qing Guo
  • Patent number: 11598775
    Abstract: A rapid immunoassay method and apparatus for detecting foot and mouth disease virus are disclosed. The method and test device permit pen-side testing of animals and provide test results within a relatively short time period. In a preferred embodiment, the method and apparatus provide a means for differentiating between FMDV-infected and FMDV-vaccinated animals.
    Type: Grant
    Filed: July 9, 2018
    Date of Patent: March 7, 2023
    Assignee: Princeton BioMeditech Corporation
    Inventors: Jemo Kang, Ki-Yong Jang