Abstract: Embodiments of the present disclosure are directed to systems, apparatuses, devices and methods for tissue cultivating. Such systems may comprise a plurality of bioreactors, each bioreactor comprising a bag having a mandrel tube arranged therein, and a fluid management system for managing fluid flow among the plurality of bioreactors. The fluid management system can include at least one intra-luminal pump, configured to flow intra-luminal fluid in a first direction in the bioreactors, at least one media pump, configured to flow biomedia fluid in the bioreactors in a second direction opposite to the first direction, and a plurality of valves and/or clamps to effect at least one of filling, flowing, cessation of flow, and draining of at least one of the intra-luminal fluid and biomedia fluid tubes among the bioreactors.

Abstract: A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.

Abstract: A solid-liquid boundary detection device 20 is a device for detecting a boundary 141 between a solid and a liquid in a test tube 14, and includes a surface illumination 21, an imaging unit 22, and a boundary detection processor 31. The surface illumination 21 illuminates the test tube 14 from outside. The imaging unit 22 is arranged on an opposite side of the test tube 14 from the surface illumination 21, and captures an image including the boundary 141. The boundary detection processor 31 detects the boundary 141 from the image captured by the imaging unit 22.

Abstract: Provided herein is a method for incubating living cells, in accordance with principles of the disclosure, may include the steps of: (a) providing a pressurized gas to a medium reservoir, the pressurized gas providing an impetus that moves a growth medium in the medium reservoir to a bioreactor chamber via a first incoming fluid line connecting the medium reservoir to the bioreactor chamber; (b) simultaneously or subsequently to step (a), providing a pressurized gas to a cell reservoir holding a suspension of the living cells, the pressurized gas providing an impetus that moves the suspension to the bioreactor chamber via a second incoming fluid line connecting the cell reservoir to the bioreactor chamber; and (c) incubating the growth medium and the living cells in the bioreactor chamber, under conditions compatible with cell viability.

Abstract: A bioreactor includes a container (1) composed of at least one side wall (11), a bottom wall (12) and an opening (13) closed by a cap (14), such to identify an inner chamber. An intermediate wall (2) is inside the inner chamber, to divide the inner chamber in an upper chamber (31) and a lower chamber (32). The intermediate wall (2) has at least one hole (21). The intermediate wall (2) has at least one region resting on the bottom wall and/or the side wall and provides at the hole (21) at least one housing seat (26) for a membrane (22).

Abstract: The present invention provides a cell cultivation module comprising a polymer porous film and a casing that has two or more culture medium inflow/outflow ports and accommodates the polymer porous film, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the polymer porous film is accommodated within the casing.

Abstract: A system for removing undesirable compounds from contaminated air includes a biofilter having sintered glass media. Hydrogen sulfide is removed from contaminated air by passing the contaminated air through the biofilter.

Abstract: A microcavity dish (10) for cultivating cells includes a dish body including a sidewall (16) that encloses a cell culture chamber within the dish body. The dish body has a top and a bottom (12). The bottom includes a cell culturing substrate comprising an array of microcavities (46). The sidewall includes a transition portion (30) that divides the sidewall into an upper portion and a lower portion that is offset inward relative to the upper portion defining a liquid medium delivery surface (26) that extends at least partially along an interior surface (28) of the sidewall and slopes toward the bottom.

Abstract: Generation of energy and storage of energy for subsequent use is provided by electromethanogenesis of carbon dioxide into a fuel gas and the storage of the fuel gas for subsequent use. An electromethanogenic reactor includes an anode conductor and a cathode conductor wherein the cathode conductor includes submicron to micron scale pores. Electromethanogenesis microbes and/or enzymes are located in the micron scale pores of the cathode electrode conductor. Carbon dioxide is introduced into the electromethanogenic reactor and the electromethanogenesis microbes/enzymes and the carbon dioxide interact and produce a fuel gas. The fuel gas is stored for subsequent use, for example use in power generation.

Abstract: The disclosure relates to a liquid reservoir for a food waste recycling appliance. The liquid reservoir includes a reservoir body with a hollow interior and having an exterior surface bounding an interstitial space. A food waste through opening can pass through the interstitial space. The reservoir body can also include a channel on the exterior surface.

Abstract: An automatic processing device of culture plates (2) for microbiological samples, wherein the processing device (1) includes a support frame (3); a slide (4) provided with a seating (5) configured for removably housing a culture plate (2) and movably mounted on the support frame (3) so as to be selectively displaceable between a first loading position, a plurality of image-acquiring positions, and a first unloading; a camera (6) of a linear type, provided with an optic (7) of a telecentric type and a trilinear sensor, and arranged according to a vertical axis (8) such as to acquire, at an image-acquiring zone, a multiplicity of linear images of corresponding linear portions of an upper surface of the culture plate (2), during the displacing of the slide (4); a first lighting device (11) orientated such as to illuminate the linear portions of an upper surface of the culture plate (2); an advancing device (14) of the slide (4) configured such as to enable obtaining a constant and substantially vibration-free ad

Abstract: The present invention provides a method for culturing cells in a cell culture container having a base section, a top section arranged in parallel with the top section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the container is composed of a flexible material, comprising culturing cells in a culture medium in the cell culture container.

Abstract: An inverted conical bioreactor is provided for growing cells or microorganisms. The bioreactor has an internal space and a perforated barrier within the vessel, through which a liquid may flow, where cells or microorganisms cannot pass through the perforated barrier. The perforated barrier divides the internal space of the bioreactor into a first chamber and a second chamber. Cells are grown within the second chamber and can be perfused by re-circulating the liquid, for example a growth medium, through the bioreactor. Various inlet ports and outlet ports allow controlling the parameters of flow of the growth medium.

Abstract: The present invention relates to a perfusion loop assembly for an ex vivo liver perfusion including: a pump for providing a fluid flow of a perfusion fluid through a first branch line and a second branch line; the first branch line being configured to provide a first portion of the perfusion fluid to the hepatic artery of the liver; the first branch line being coupled with a first gas exchanger, the second branch line being configured to provide a second portion of the perfusion fluid to the portal vein of the liver; the second branch line further including a first valve for controlling the flow of the perfusion fluid into the portal vein of the liver, a liver chamber assembly configured to hold the liver ex vivo, a liver outlet line attached to the vena cava of the ex vivo liver, at least one reservoir connected to the liver outlet and upstream from the pump.

Abstract: A cell culture system or assembly, flask, plate and dish for growing, viewing and evaluating cells; and methods of use.

Abstract: A supporting device for an image capturing device on a bioreactor, a bioreactor, and a method of use are provided. The supporting device for an image capturing device includes a window and a holder. The window has a transparent element that is transparent to electromagnetic radiation. The holder is configured to hold the image capturing device. The supporting device is held at least partially in a through-opening of a feedthrough of a container for holding fluid media containing biological material. The window seals the through-opening.

Abstract: A vitrification device for gametes or embryos, wherein, the vitrification straw comprises: a loading rod, wherein the loading rod is a metal rod; a loading strip, wherein, the loading strip is connected with one end of the loading rod. According to the present invention, the loading rod is arranged as metal rod, which avoids embrittlement fracture caused by sudden temperature change when the loading rod is taken out of liquid nitrogen, moreover, the ice crystals that form on gametes or embryos when the loading rod floats out of the surface of liquid nitrogen, affecting the safety of gametes or embryos, the metal material used by this invention can increase the weight of the loading rod, preventing it from floating up in the liquid nitrogen, hence improve safety of gametes or embryos.

Abstract: The invention provides a device for growing cells—referred to as a cassette. The cell culturing device includes a housing that contains a lid having an optically clear window; a fluid distribution channel; a sample injection port fluidically connected to the fluid distribution channel; a base housing a porous media pad; and a media injection port fluidically connected to the media pad. The lid mates to the base to form a sterile seal; the fluid distribution channel is disposed over the media pad, which is viewable through the optical window; and sample fluid introduced into the fluid distribution channel is distributed evenly to the media pad, e.g., via a plurality of channels. The invention also provides kits that include cassettes of the invention and a tube set.

Abstract: Methods and apparatus of bioreactors for therapeutic cells manufacturing are provided herein. In some embodiments, a bioreactor includes: an upper bioreactor reservoir configured to perform multiple cell therapy manufacturing process steps including genetic modification and expansion to a plurality of cells disposed therein, wherein the upper bioreactor reservoir includes a plurality of ports for delivering fluids into and out of the upper bioreactor reservoir; a lower bioreactor compartment configured to hold a suspension comprising a molecular species; and a membrane disposed between the lower bioreactor compartment and the upper bioreactor reservoir, wherein the membrane includes a plurality of micro-straws extending through the membrane and into the upper bioreactor reservoir to transfect the plurality of cells with the molecular species.

Abstract: A system for quickly determining antibiotic sensitivity of a microorganism comprising a triangular-shaped plate and cover for culturing, recovering, and re-suspending recovered microbial colonies and a second triangular-shaped plate comprising a non-liquid medium cut with concentric trenches over which the re-suspended microbial colonies are distributed by centrifugation and contacted with antimicrobial strips or disks.