Patents Examined by David M. Naff
  • Patent number: 7648831
    Abstract: The purpose of the present invention is to provide a process for protection of enzymatic biocatalysts, constituted by a primary support, wrapped by a secondary matrix. More particularly, the invention is embodied as a bioreactor with a configuration that preserves the physical integrity and catalytic activity of the biocatalyst after long runs, and at the same time allows an easy separation of the protected biocatalyst from the solid, precipitated during the reaction course. Still more particularly, the invention refers to the use of the bioreactor herein described for the enzymatic synthesis of ?-lactam antibiotics, including the insoluble enzymatic catalysts, which have a primary matrix for immobilization involved by a secondary matrix, which is able to preserve 100% of the catalyst physical integrity and approximately 100% of its catalytic activity.
    Type: Grant
    Filed: November 24, 2003
    Date of Patent: January 19, 2010
    Assignees: Fundacao Universidade Federal de Sao Carlos, Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
    Inventors: Roberto de Campos Giordano, Raquel de Lima Camargo Giordano, Andrea Lopes de Oliveira Ferreira
  • Patent number: 7645608
    Abstract: A device for storing, rehydrating, transferring, and streaking a stored freeze dried specimen comprises a three piece construction including a collection vial used to store a freeze dried specimen, a capsule including a reservoir with hydrating fluid retained by a frangible membrane, and an adapter fluidly linking the capsule to the collection vial. The adapter is configured to fluidly link to the capsule to the collection vial via leak-proof couplings to create a closed system of specimen, hydrating fluid, and a small quantity of trapped air. The fluid communication between the vial and the capsule is accomplished through the adapter via an internal lumen longitudinally traversing the adapter. The adapter and capsule combination can also serve as a transfer pipette once the hydration operation is complete to withdraw the specimen from the collection vial and transfer the specimen to a petri dish or other culture medium.
    Type: Grant
    Filed: August 15, 2005
    Date of Patent: January 12, 2010
    Assignee: PML Microbiologicals, Inc.
    Inventor: Nathan Greene
  • Patent number: 7645593
    Abstract: The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.
    Type: Grant
    Filed: October 2, 2008
    Date of Patent: January 12, 2010
    Assignee: Litron Laboratories, Ltd.
    Inventors: Stephen D. Dertinger, Sian E. Cairns, Svetlana L. Avlasevich, Dorothea K. Torous
  • Patent number: 7642077
    Abstract: This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, ?-hydroxylysine, and ?-hydroxy-?-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.
    Type: Grant
    Filed: December 7, 2004
    Date of Patent: January 5, 2010
    Assignee: Genencor International, Inc.
    Inventors: Risha Bond, Michael C. Jewett, Joseph C. McAuliffe, Donald E. Ward, II
  • Patent number: 7635573
    Abstract: The present invention provides a new and improved method for quantitative determination and/or identification of proteins in a sample. In one aspect, the invention provides a mass spectroscopic method for comparing protein levels in two or more samples by differentially isotopically labeling each sample's proteins' N- or C-termini. In another aspect, the invention provides a mass-spectroscopic method for identifying a sample as source for a protein from a mixture of two or more samples by differentially isotopically labeling each sample's proteins' N- or C-termini.
    Type: Grant
    Filed: December 22, 2005
    Date of Patent: December 22, 2009
    Assignee: Agilent Technologies, Inc.
    Inventor: Steven M. Fischer
  • Patent number: 7632672
    Abstract: An electrochemical sensor system and membrane and method thereof for increased accuracy and effective life of electrochemical and enzyme sensors.
    Type: Grant
    Filed: June 1, 2005
    Date of Patent: December 15, 2009
    Assignee: Instrumentation Laboratory Co.
    Inventors: Prasad Pamidi, Sohrab Mansouri, Melanie Shin, Vasile Cosofret, Clarke Xu
  • Patent number: 7629159
    Abstract: The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed enrichment of degrading bacteria, which contains a porous material provided as a support on which degrading bacteria A capable of degrading at least one organic contaminant and degrading bacteria B capable of degrading another organic contaminant are enriched, is produced. The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of ?-Proteobacteria CDB21.
    Type: Grant
    Filed: January 20, 2006
    Date of Patent: December 8, 2009
    Assignees: National Institute for Agro-Environmental Sciences Independent Administration Institute, Kowa Co. Ltd.
    Inventors: Kazuhiro Takagi, Naoki Harada, Yuuichi Yoshioka
  • Patent number: 7625728
    Abstract: Effective processes are provided for the production of xylitol and ethanol and other products from solutions derived from lignocellulose-containing material in biomass. The solutions can be hydrolyzed or partially hydrolyzed before being fermented with microbes. The fermented solution can be distilled and can be subsequently separated, such as, by chromatographic separation, membrane separation, etc. The recovered xylitol solution can be crystallized to provide pure xylitol crystals.
    Type: Grant
    Filed: June 30, 2006
    Date of Patent: December 1, 2009
    Assignee: Danisco Sweeteners Oy
    Inventors: Olli-Pekka Eroma, Heikki Heikkila, Heikki Ojamo, Päivi Sarmala, Göran Hyöky, Leena Rahkila, Marja-Leena Sarkki, Tapio Viljava
  • Patent number: 7625727
    Abstract: The present invention discloses the manufacture of MPA by fermentation under optimal fermentation parameters using a new strain of Penicillium arenicola.
    Type: Grant
    Filed: October 5, 2004
    Date of Patent: December 1, 2009
    Assignee: Biocon Limited
    Inventors: Surekha Prabhu, Pinakee Devi Sharma, Sanjay Tiwari, Ramakrishnan Melarkode, Ramavana Gururaja, Shrikumar Suryanarayan
  • Patent number: 7615366
    Abstract: The present invention relates to a novel microorganism Bacillus amyloliquefaciens KTGB0202 and a method for controlling plant pathogens using the same. More particularly, the present invention relates to a novel microorganism Bacillus amyloliquefaciens KTGB0202 (accession number: KCTC 10564BP) bacterium which has an excellent control effect against crop powdery mildew and a broad spectrum of antifungal activity against plant pathogenic fungi and inhibits tobacco mosaic virus infection, as well as an eco-friendly bacterial culture broth for controlling powdery mildew, which contains the same. Also, the present invention relates to antifungal substance KTGB0202AFO1 obtained by extraction and purification from the Bacillus amyloliquefaciens KTGB0202 bacteria. The inventive Bacillus amyloliquefaciens KTGB0202 bacteria have a broad spectrum of antifungal activity, and are excellent in activity continuance and used for controlling various plant pathogens, including powdery mildew.
    Type: Grant
    Filed: December 7, 2004
    Date of Patent: November 10, 2009
    Assignee: KT & G Co., Ltd.
    Inventors: Kab-Sig Kim, Woon-Hyung Yeo
  • Patent number: 7611835
    Abstract: A process for preparing high stability, high activity biocatalytic materials is disclosed and processes for using the same. The process involves coating of a material or fiber with enzymes and enzyme aggregate providing a material or fiber with high biocatalytic activity and stability useful in heterogeneous environments. In one illustrative approach, enzyme “seeds” are covalently attached to polymer nanofibers followed by treatment with a reagent that crosslinks additional enzyme molecules to the seed enzymes forming enzyme aggregates thereby improving biocatalytic activity due to increased enzyme loading and enzyme stability. This approach creates a useful new biocatalytic immobilized enzyme system with potential applications in bioconversion, bioremediation, biosensors, and biofuel cells.
    Type: Grant
    Filed: September 30, 2005
    Date of Patent: November 3, 2009
    Assignee: Battelle Memorial Institute
    Inventors: Jungbae Kim, Ja Hun Kwak, Jay W. Grate
  • Patent number: 7611701
    Abstract: A process for the preparation of an enzyme-containing granulate is disclosed where an aqueous enzyme-containing liquid is mixed with an edible carbohydrate-based solid carrier, such as starch, mechanically processed into granules, and subsequently dried. This enzyme granulate is suitable for the manufacture of animal feed compositions by mixing feed ingredients with the granulate, treating with steam and pelleting. The compositions optimally show improved enzyme stability during the pelleting process.
    Type: Grant
    Filed: October 25, 2002
    Date of Patent: November 3, 2009
    Assignee: BASF Aktiengesellschaft
    Inventors: Rudolph Carolus Maria Barendse, Gabriel Marinus Henricus Meesters, Hans-Peter Harz
  • Patent number: 7611878
    Abstract: The present invention relates generally to high stability, high activity biocatalytic materials and processes for using the same. The materials comprise enzyme aggregate coatings having high biocatalytic activity and stability useful in heterogeneous environment. These new materials provide a new biocatalytic immobilized enzyme system with applications in bioconversion, bioremediation, biosensors, and biofuel cells.
    Type: Grant
    Filed: September 30, 2005
    Date of Patent: November 3, 2009
    Assignee: Battelle Memorial Institute
    Inventors: Jungbae Kim, Ja Hun Kwak, Jay W. Grate
  • Patent number: 7611831
    Abstract: Methods and devices are provided for reducing the concentration of low molecular weight compounds in a biological composition containing cells while substantially maintaining a desired biological activity of the biological composition. The device comprises highly porous adsorbent particles, and the adsorbent particles are immobilized by an inert matrix. The matrix containing the particles is contained in a housing, and the particles range in diameter from about 100 ?m to about 1500 ?m. The device can be used to adsorb and remove a pathogen-inactivating compounds from a biological composition such as a blood product.
    Type: Grant
    Filed: October 4, 2005
    Date of Patent: November 3, 2009
    Assignee: Cerus Corporation
    Inventor: Derek J. Hei
  • Patent number: 7608435
    Abstract: The invention relates to a microorganism, obtained by treating Corynebacterium ammoniagenes KCCM 10488 producing 5?-Xanthylic acid as parent strain with UV radiation and mutation Derivatives such as N-methyl-N?nitro-N-nitrosoguanidine (NTG), Having a resistance to 5-fluorotryptophan which enhances Biosynthesis of N5-N10-tetrahydrofolate used for transferring Two formyl group during the process of puring biosynthesis, making It possible to accumulate 5?xanthylic acid in culture medium at a high Yield and high concentration rate same period of fermentation.
    Type: Grant
    Filed: November 18, 2004
    Date of Patent: October 27, 2009
    Assignee: CJ Cheiljedang Corp.
    Inventors: Young-Hoon Park, Jea-Young Chang, Jin-Nam Lee, Ki-Hoon Oh, Jeong-Hwan Kim, Yoon-Suk Oh, Jae-Ick Sim
  • Patent number: 7608283
    Abstract: The present invention provides a method of extracting organic substances present in coral, which consists in treating the coral with a fluid or a mixture of fluids in the supercritical state without modifying the crystalline structure of said coral, at a temperature of less than 270° C. and at a pressure which is much higher than the critical pressure of said fluid or mixture of fluids, for example of the order of at least 3 times, and preferably at least 5 times said critical pressure. It also provides coral obtained by said method and bone substitutes fabricated from said coral.
    Type: Grant
    Filed: December 6, 2004
    Date of Patent: October 27, 2009
    Assignee: Biocoral Inc.
    Inventors: Yann Lepetitcorps, Jean-Christophe Fricain, Vincent Souillac, Alain Largeteau, Roland Schmitthaeusler
  • Patent number: 7604817
    Abstract: A device and method for tissue engineering is disclosed. More particularly this invention relates to a bioabsorbable device and a method of its use which promotes controlled new tissue in-growth into voids or cavities occupied by the device as portions of the device are selectively absorbed within a host thereby minimizing collapse of surrounding, pre-existing host tissue into the engineered site.
    Type: Grant
    Filed: September 3, 2003
    Date of Patent: October 20, 2009
    Assignee: Ethicon Endo-Surgery, Inc.
    Inventors: Chin-Feng Yi, Anna Gosiewska, Susan Roweton
  • Patent number: 7601525
    Abstract: The present invention provides copper capillary alginate gels stabilized with barium, chitosan, its derivates, or a combination thereof. These stabilized gels are useful as scaffolds for containing, growing, or regenerating biological agents and cells for in vivo or in vitro use.
    Type: Grant
    Filed: March 7, 2005
    Date of Patent: October 13, 2009
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Christopher D. Batich, Bradley Jay Willenberg, Takashi Hamazaki, Naohiro Terada
  • Patent number: 7597885
    Abstract: The present invention relates to an improved scaffold which is constructed to mimic the architecture of the native hair follicle. The present invention also relates to the use of specific compositions and methods of manufacture to produce scaffolds that combine biocompatibility with the desired rates of bioabsorption. In another embodiment, the present invention relates to a process for manufacturing a biomimetic hair follicle graft and a method for seeding the graft with cells and implanting the graft into the skin where the growth of a new hair shaft is desired. A further embodiment of the present invention relates to a method for hair multiplication in which cells are multiplied in culture and aliquoted into a multitude of bioabsorbable scaffolds in combination with cultured keratinocytes or other allogenic cells.
    Type: Grant
    Filed: March 26, 2004
    Date of Patent: October 6, 2009
    Assignee: Aderans Research Institute, Inc.
    Inventors: Thomas H. Barrows, Stephen A. Cochran, Bryan Marshall
  • Patent number: 7585499
    Abstract: Positively-charged initiator polymers having a polymerization initiator group and a cationic portion are provided. The initiator polymers can be used with a polymerizable material for the formation of a polymeric matrix on a surface. The initiator polymers are particularly useful for cell encapsulation using macromers.
    Type: Grant
    Filed: April 10, 2003
    Date of Patent: September 8, 2009
    Assignee: SurModics, Inc.
    Inventors: Stephen J. Chudzik, Ronald F. Ofstead, Dale G. Swan