Patents Examined by Delia M. Ramirez
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Patent number: 11692208Abstract: Among other things, the present disclosure provides biosynthesis polypeptides, methods, and non-naturally occurring microbial organisms for preparing various compounds such as 1,5-pentanediol, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, and 2-keto carboxylic acids.Type: GrantFiled: September 16, 2022Date of Patent: July 4, 2023Assignee: ZYMOCHEM, INC.Inventors: Harshal Akshay Chokhawala, Jonathan Kuchenreuther, Jorge Alonso-Gutiérrez, Yi-Shu Tai
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Patent number: 11685768Abstract: The present disclosure relates to methods for production of (Z)-11-hexadecen-1-ol in a yeast cell using desaturases and fatty acyl-CoA reductase. Also disclosed are methods for production of (Z)-11-hexadecenal in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecen-1-yl acetate in a yeast cell. The disclosure also provides for nucleic acid constructs and yeast cells useful for performing the present methods, as well as to pheromone compositions.Type: GrantFiled: June 24, 2016Date of Patent: June 27, 2023Assignee: Danmarks Tekniske UniversitetInventors: Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
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Patent number: 11634739Abstract: A regulation method for preparing ?-polyglutamic acid by sludge substrate fermentation includes: 1) extraction of glutamic acid from sludge protein (high pressure hydrothermal treatment, gravity pressure filtration treatment), 2) secondary metabolic synthesis of ?-polyglutamic acid (activation of domesticated strains and secondary metabolic fermentation strains); and 3) preparation of pure ?-polyglutamic acid (acidification, centrifugation, filtration, precipitation based on polar repulsion, purification, impurity removal and drying). The present invention realizes a recycling of high-value carbon and nitrogen sources of sludge without secondary pollution, and has advantages of simplified operation, good feasibility, and low preparation cost. The synthesized ?-polyglutamic acid has high economic value and broad application prospect.Type: GrantFiled: September 22, 2020Date of Patent: April 25, 2023Assignee: TONGJI UNIVERSITYInventors: Bin Dong, Xin Li
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Patent number: 11618911Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.Type: GrantFiled: June 2, 2022Date of Patent: April 4, 2023Assignee: Codexis, Inc.Inventors: Haibin Chen, Steven J. Collier, Jovana Nazor, Joly Sukumaran, Derek Smith, Jeffrey C. Moore, Gregory Hughes, Jacob Janey, Gjalt W. Huisman, Scott J. Novick, Nicholas J. Agard, Oscar Alvizo, Gregory A. Cope, Wan Lin Yeo, Stefanie Ng Minor
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Patent number: 11584921Abstract: Provided herein is a stabilized DNase I polypeptide containing a non-standard amino acid that maintains enzymatic activity even under harsh conditions, such as reducing environments. The stabilized DNase I polypeptide has enzymatic activity in reducing environments that is higher than a corresponding DNase I polypeptide without the non-standard amino acids under the same conditions. Also provided herein are polynucleotides encoding the stabilized DNase I polypeptide, cells for expressing and/or producing the stabilized DNase I polypeptide, and methods of use of the stabilized DNase I polypeptide.Type: GrantFiled: January 8, 2021Date of Patent: February 21, 2023Assignee: GRO Biosciences Inc.Inventors: Daniel J. Mandell, Christopher J. Gregg, Peter B. Stranges
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Patent number: 11578345Abstract: Provided is process for the synthesis of at least one functionalized organic polysulfide.Type: GrantFiled: December 21, 2017Date of Patent: February 14, 2023Assignee: Arkema FranceInventors: Georges Fremy, Arnaud Masselin
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Patent number: 11555213Abstract: The present disclosure relates to modified homoserine dehydrogenase and a method for producing a homoserine-derived L-amino acid using the same.Type: GrantFiled: September 10, 2021Date of Patent: January 17, 2023Assignee: CJ CHEILJEDANG CORPORATIONInventors: Hyo Jin Kim, Lan Huh, Sang Jo Lim, Hyun Ah Kim, Hyoung Joon Kim, Chang il Seo, Seung Bin Lee, Ji Sun Lee
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Patent number: 11549105Abstract: The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.Type: GrantFiled: December 21, 2018Date of Patent: January 10, 2023Assignee: CARBIOSInventors: Alain Marty, Sophie Duquesne, Marie Guicherd, Marc Gueroult, Isabelle Andre
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Patent number: 11535663Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.Type: GrantFiled: July 22, 2021Date of Patent: December 27, 2022Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Shawn Lawrence, Amy Johnson, Meghan Casey, Jaimie Mastrogiacomo, Shunhai Wang, Ning Li
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Patent number: 11535873Abstract: Processes, systems and microorganisms are described herein for producing glycolate from ethylene glycol. The processes generally comprise supplying a fermentation broth into a fermentation vessel, wherein the fermentation broth comprises ethylene glycol and a microorganism having a functional metabolic pathway for utilizing ethylene glycol as a carbon source. In a growth phase, an oxygen-containing gas is injected into the fermentation broth to provide oxygen bio-availability conditions to promote cell growth of the microorganism and limit accumulation of glycolate in the fermentation broth. In a production phase, an oxygen-containing gas is injected into the fermentation broth to provide oxygen bio-availability conditions to promote production of glycolate from ethylene glycol by the microorganism and accumulation of the glycolate in the fermentation broth, to produce a glycolate enriched broth.Type: GrantFiled: September 6, 2018Date of Patent: December 27, 2022Assignee: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTOInventors: Aditya Vikram Pandit, Radhakrishnan Mahadevan
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Patent number: 11530428Abstract: The present invention provides for heterologous expression of polypeptides encoded by wild-type and condon-optimized variants of cbh1 and/or cbh2 from the fungal organisms Talaromyces emersonii (T. emersonii), Humicola grisea (H. grisea), Thermoascus aurantiacus (T. aurantiacus), and Trichoderma reesei (T. reesei) in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.Type: GrantFiled: May 25, 2016Date of Patent: December 20, 2022Inventors: Riaan Den Haan, Emile Van Zyl, Danie LaGrange
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Patent number: 11512319Abstract: The present invention provides a recombinant Corynebacterium glutamicum producing N-acetylglucosamine and use thereof. The recombinant Corynebacterium glutamicum is obtained by overexpressing, in Corynebacterium glutamicum, the transcription factor SugR derived therefrom. The recombinant Corynebacterium glutamicum of the present invention increases the production of acetylglucosamine to up to 26 g/L, and lays a foundation for further metabolic engineering of Corynebacterium glutamicum to produce glucosamine.Type: GrantFiled: January 7, 2020Date of Patent: November 29, 2022Assignees: JIANGNAN UNIVERSITY, SHANDONG RUNDE BIOTECHNOLOGY CO., LTD.Inventors: Jianxing Lu, Long Liu, Jian Chen, Changfeng Liu, Xueqin Lv, Guocheng Du, Jianghua Li, Chen Deng, Jiangong Lu
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Patent number: 11512304Abstract: Provided herein are engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of amino acids such as L-tyrosine to produce tyramine or catalyzing the decarboxylation of L-DOPA to produce dopamine. Also provided are the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, recombinant host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing tyramine or dopamine using the engineered decarboxylase polypeptides. Compared to the wild type decarboxylase, the engineered polypeptide provided by this disclosure has better activity and/or stability. The use of the engineered polypeptides for the preparation of tyramine or dopamine reduces the production cost and has a good industrial application prospect.Type: GrantFiled: April 1, 2019Date of Patent: November 29, 2022Assignee: ENZYMASTER (NINGBO) BIO-ENGINEERING CO., LTD.Inventors: Haibin Chen, Yong Koy Bong, Qing Xu, Dongxiao Hu, Chengxiao Zhang, Baoqin Cai
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Patent number: 11505833Abstract: Novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have been identified herein in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides.Type: GrantFiled: November 24, 2020Date of Patent: November 22, 2022Assignee: CELL SIGNALING TECHNOLOGY, INC.Inventors: Klarisa Rikova, Herbert Haack, Laura Sullivan, Ailan Guo, Anthony Possemato, Joan MacNeill, Ting-Lei Gu, Jian Yu
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Patent number: 11505838Abstract: An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biochemical production. In particular, after saccharification and prior to a sugar conversion process, a sugar/carbohydrate stream is removed from a saccharified stream. The sugar/carbohydrate stream includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose can be produced, with the such sugar stream being available for biochemical production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein and/or fiber. Sugar stream production occurs on the front end of the system and method.Type: GrantFiled: April 5, 2018Date of Patent: November 22, 2022Assignee: Fluid Quip Technologies, LLCInventors: Neal Jakel, Albert Pollmeier
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Patent number: 11499143Abstract: Provided are an amadoriase that is less likely to be influenced by oxygen concentration and a method and a reagent kit for measurement of HbA1c using such amadoriase. Provided are an amadoriase that is obtained via substitution of one or more amino acid residues at a position or positions corresponding to the position(s) selected from the group consisting of positions 280, 267, 269, 54, and 241 of the amadoriase derived from the genus Coniochaeta, a method for measurement of HbA1c, a reagent kit for measurement, and a sensor using such amadoriase. The modified amadoriase according to the invention has a lowered oxidase activity and an enhanced dehydrogenase activity, and this enables the use of an electron mediator, and this reduces the influence of oxygen concentration. Thus, HbA1c can be measured with high sensitivity.Type: GrantFiled: October 23, 2015Date of Patent: November 15, 2022Assignee: Kikkoman CorporationInventors: Yosuke Masakari, Airi Komatsuzaki, Atsushi Ichiyanagi
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Patent number: 11499146Abstract: Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more Bacillus gibsonii-clade subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.Type: GrantFiled: June 17, 2016Date of Patent: November 15, 2022Assignee: DANISCO US INC.Inventors: Lilia Maria Babe, Richard R. Bott, David A. Estell, Harm Mulder, Frits Goedegebuur, Jian Yao, Sina Pricelius, Miles Scotcher
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Patent number: 11485987Abstract: A method for catalytically converting a dihydrotetrazine 1 into a tetrazine 2, wherein the dihydrotetrazine 1 comprises a first R group and a second R group, wherein the first R group is a substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, or heteroatom-containing group, and the second R group is selected from the group consisting of H and substituted or unsubstituted aryl, heteroaryl, alkyl, alkenyl, alkynyl, carbonyl, and heteroatom-containing groups; wherein the method comprises (a) providing the dihydrotetrazine 1 in a reaction medium, and (b) adding an enzyme as a catalyst and an oxidant to the reaction medium, whereby the dihydrotetrazine 1 is converted to the tetrazine 2.Type: GrantFiled: December 23, 2020Date of Patent: November 1, 2022Assignee: University of DelawareInventors: Joseph M. Fox, Colin Thorpe, Amanda M. Tallon, Yixin Xie, William Trout, Ashlyn S. Cantrel, Julia E. Rosenberger
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Patent number: 11479761Abstract: The invention relates to the field of genetic engineering tools, methods and techniques for gene or genome editing. Specifically, the invention concerns isolated polypeptides having nuclease activity, host cells and expression vectors comprising nucleic acids encoding said polypeptides as well as methods of cleaving and editing target nucleic acids in a sequence-specific manner. The polypeptides, nucleic acids, expression vectors, host cells and methods of the present invention have application in many fields of biotechnology, including, for example, synthetic biology and gene therapy.Type: GrantFiled: April 30, 2019Date of Patent: October 25, 2022Assignee: WAGENINGEN UNIVERSITEITInventor: John Van Der Oost
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Patent number: 11479778Abstract: The present invention relates to the technical field of gene engineering and particularly relates to a method for constructing non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield, recombinant strain constructed by method, and application of recombinant strain. According to the present invention, a diacylglycerol acyltransferase gene (DGAT) is overexpressed in Mucor circinelloides WJ11 by a homologous recombination technology, and exogenous oil is added for fermentation, such that the non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield is constructed. Compared with the control strain Mc2075, the fat yield of the Mucor circinelloides is increased; and when the diacylglycerol acyltransferase (DGAT) is transformed into the uracil defective type of Mucor circinelloides WJ11, the fatty acid composition changes after fermentation, and the lipid content may reach 53% of dry cell weight after the fermentation condition is optimized.Type: GrantFiled: November 30, 2020Date of Patent: October 25, 2022Assignee: SHANDONG UNIVERSITY OF TECHNOLOGYInventors: Yuanda Song, Caili Sun, Huaiyuan Zhang, Wu Yang, Meiling Chen