Patents Examined by Eggerton Campbell
  • Patent number: 6294326
    Abstract: Provided herein is a method for detecting the presence of a target nucleic acid sequence in a sample. The present method uses a combination of dual labeled probes and heterogeneous detection methods.
    Type: Grant
    Filed: November 7, 1997
    Date of Patent: September 25, 2001
    Assignee: Abbott Laboratories
    Inventors: John J. Carrino, John R. Ertl, John A. Salituro, Paul M. Jung
  • Patent number: 6291191
    Abstract: The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change.
    Type: Grant
    Filed: December 2, 1999
    Date of Patent: September 18, 2001
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Michael W. Pantoliano, Eugenio C. Petrella, Alexander W. Rhind, Francis R. Salemme, Barry A. Springer
  • Patent number: 6291664
    Abstract: A method of locating an inhibitory/instability sequence or sequences within the coding region of an mRNA and modifying the gene encoding that mRNA to remove these inhibitory/instability sequences by making clustered nucleotide substitutions without altering the coding capacity of the gene is disclosed. Constructs containing these mutated genes and host cells containing these constructs are also disclosed. The method and constructs are exemplified by the mutation of a Human Immunodeficiency Virus-1 Rev-dependent gag gene to a Rev independent gag gene. Constructs useful in locating inhibitory/instability sequences within either the coding region or the 3′ untranslated region of an mRNA are also disclosed. The exemplified constructs of the invention may also be useful in HIV-1 immunotherapy and immunoprophylaxis.
    Type: Grant
    Filed: October 8, 1999
    Date of Patent: September 18, 2001
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: George N. Pavlakis, Barbara K. Felber
  • Patent number: 6291192
    Abstract: The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change.
    Type: Grant
    Filed: January 5, 2000
    Date of Patent: September 18, 2001
    Assignee: 3-Dimensional Pharmaceuticals Inc.
    Inventors: Michael W. Pantoliano, Eugenio C. Petrella, Francis R. Salemme, Barry A. Springer
  • Patent number: 6291185
    Abstract: Double stranded nucleic acid is denatured by subjecting a solution thereof to a voltage applied between electrodes spaced by no more than 1.5 mm in a time not previously achievable in electrochemical denaturation. PCR is practised isothermally by periodic application of voltage to produce denaturation. Electrochemical cells and kits for use in the process are provided.
    Type: Grant
    Filed: March 6, 2000
    Date of Patent: September 18, 2001
    Assignee: Affymetrix, Inc.
    Inventor: Duncan R. Purvis
  • Patent number: 6291178
    Abstract: A method and apparatus for the preservation of a saliva sample for use in subsequent quantitative chemical assays. The method involves collecting a saliva sample at a location, directly into a specimen cup. The specimen cup contains a predetermined volume of aqueous solution of pH buffered saline and enzymatic inhibitor and is optionally adapted with a constituent compound specific, qualitative test unit.
    Type: Grant
    Filed: August 30, 1999
    Date of Patent: September 18, 2001
    Inventor: David R. Schneider
  • Patent number: 6287777
    Abstract: The invention provides isolated nucleic acids molecules, designated NPG-1 nucleic acid molecules, which encode polypeptides involved in the modulation of a response in a tumor cell. The invention also provides antisense nucleic acid molecules, expression vectors containing NPG-1 nucleic acid molecules, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which an NPG-1 gene has been introduced or disrupted. The invention still further provides isolated NPG-1 polypeptides, fusion polypeptides, antigenic peptides, and anti-NPG-1 antibodies. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.
    Type: Grant
    Filed: August 10, 1999
    Date of Patent: September 11, 2001
    Assignee: Beth Israel Deaconess Medical Center
    Inventors: Arthur J. Sytkowski, Meiheng Yang
  • Patent number: 6287762
    Abstract: Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.
    Type: Grant
    Filed: June 9, 1997
    Date of Patent: September 11, 2001
    Assignee: Rhone-Poulenc Rorer S.A.
    Inventors: Joël Crouzet, Daniel Scherman, Pierre Wils
  • Patent number: 6280945
    Abstract: A method of determining the presence of a nucleic acid analyte from a sample comprising binding a biotinylated capture oligonucleotide to a solid phase non-covalently coated with streptavidin or an avidin derivative. The capture oligonucleotide is contacted with and selectively hybridized to, under stringent hybridization conditions, a nucleic acid analyte to form a detectable hybridization complex.
    Type: Grant
    Filed: January 6, 2000
    Date of Patent: August 28, 2001
    Assignee: Saigene Corporation
    Inventor: Jack U'ren
  • Patent number: 6280933
    Abstract: Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
    Type: Grant
    Filed: November 7, 1997
    Date of Patent: August 28, 2001
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Richard A. Mathies, Konan Peck
  • Patent number: 6277564
    Abstract: The present invention relates to rapid, reliable and effective assays for screening and identifying pharmaceutically effective compounds that specifically inhibit the biological activity of fungal GTPase proteins, particularly GTPases involved in cell wall integrity, hyphael formation, and/or other cellular functions critical to pathogenesis. Another aspect of the present invention relates to novel Candida genes and gene products.
    Type: Grant
    Filed: April 23, 1997
    Date of Patent: August 21, 2001
    Assignee: GPC Biotech Inc.
    Inventors: Vivian Berlin, Veronique Damagnez, Susan E. Smith
  • Patent number: 6277606
    Abstract: Methodology is provided for developing probes for identifying sequence differences between two related DNA populations, sets of DNA fragments or collections of restriction-endonuclease-cleaved DNA or cDNA. The method employs an initial stage to obtain a representation of both DNA populations, namely using the PCR to produce relatively short fragments, referred to as amplicons. Tester amplicons containing target DNA, sequences of interest, are ligated to adaptors and mixed with excess driver amplicons under melting and annealing conditions, followed by PCR amplification. The process may be repeated so as to greatly enrich the target DNA. Optionally, the target DNA may then be cloned and the DNA used as probes.
    Type: Grant
    Filed: March 2, 1999
    Date of Patent: August 21, 2001
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Michael Wigler, Nikolai Lisitsyn
  • Patent number: 6274315
    Abstract: Sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenase™ which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide feedstocks, and a chain terminating nucleotide. The reaction mixture also includes an unconventional nucleotide and an appropriate enzyme for degradation of nucleic acid polymers containing the unconventional nucleotide. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.
    Type: Grant
    Filed: April 24, 1998
    Date of Patent: August 14, 2001
    Assignee: Visible Genetics Inc.
    Inventors: James Leushner, Jean-Michel Lacroix, May Hui, James M. Dunn, Marina T. Larson
  • Patent number: 6274308
    Abstract: The invention relates to a method for purifying of a nucleic acid from a biological sample. The method involves contacting a biological sample containing a nucleic acid with a hydrophilic polyvinylidene fluoride (PVDF) membrane which contains pores having an average diameter less than about 0.45 &mgr;m and recovering the nucleic acid. The method can further include adding silica beads to the biological sample prior to the contacting step.
    Type: Grant
    Filed: March 16, 1999
    Date of Patent: August 14, 2001
    Assignee: New York Blood Center, Inc.
    Inventors: Dong-Hun Lee, Alfred M. Prince
  • Patent number: 6270962
    Abstract: The present invention relates to improvements in methods of DNA sequencing. In particular, the invention relates to the elimination of stops or pauses in chain termination methods of DNA sequencing by the addition of nitrogen-containing organic compounds such as betaine, trimethylamine N-oxide and dimethylglycine. The invention also provides for DNA sequencing kits containing these compounds. The invention also provides for improvements in other laboratory procedures using DNA polymerases, such as polymerase chain reaction (PCR).
    Type: Grant
    Filed: February 2, 1998
    Date of Patent: August 7, 2001
    Assignee: The Regents of the University of California
    Inventors: Michael Chamberlin, Daniel Mytelka
  • Patent number: 6268158
    Abstract: The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change.
    Type: Grant
    Filed: December 10, 1999
    Date of Patent: July 31, 2001
    Assignee: 3-Dimensional Pharmaceuticals, Inc.
    Inventors: Michael W. Pantoliano, Eugenio C. Petrella, Francis R. Salemme, Barry A. Springer
  • Patent number: 6261781
    Abstract: A direct detection and quantitation of RNA or DNA contained in a sample is obtained by capillary electrophoresis of the RNA or DNA hybridized to a DNA or RNA probe of complementary sequence stabilized by the combination of a fluorophore terminally conjugated to the probe and a dye intercalating the RNA-DNA or DNA-DNA hybrid so formed. The RNA or DNA is quantified by measuring the total fluorescence emitted by the electrophoresed hybrid upon excitation by a laser generated light beam.
    Type: Grant
    Filed: February 4, 2000
    Date of Patent: July 17, 2001
    Assignee: Wisconsin Alumni Research Foundation
    Inventor: Jill M. Kolesar
  • Patent number: 6261795
    Abstract: The present invention provides a novel method for assaying radiation resistance of cancer cells by isolating cancer cells, irradiating the cells, incubating the cells with a labeled marker indicative of cell proliferation, measuring the amount of labeled marker incorporated into the cells in order to determine the effect of radiation on the proliferation of cells, and then determining the relative resistance of the cells to radiation. Also provided are methods of assaying radiation resistance of cancer cells which are exposed to radiation and radio-sensitizing or chemotherapeutic agents.
    Type: Grant
    Filed: May 17, 1999
    Date of Patent: July 17, 2001
    Assignee: Oncotech, Inc.
    Inventors: John P. Fruehauf, Ricardo J. Parker
  • Patent number: 6258570
    Abstract: A multiplex assay is provided for the simultaneous detection and discrimination of pathogens that cause bacterial and viral meningitis.
    Type: Grant
    Filed: April 17, 1998
    Date of Patent: July 10, 2001
    Assignee: University of Pittsburgh
    Inventors: Joseph Z. Glustein, Garth D. Ehrlich, Yingze Zhang
  • Patent number: 6255051
    Abstract: Methods for determining the presence of a target polynucleotide sequence using chemical hybridization in sequential probe and displacement complex formation with potential for signal gain prior to detection are disclosed.
    Type: Grant
    Filed: November 6, 1998
    Date of Patent: July 3, 2001
    Assignee: Mosaic Technologies Inc.
    Inventors: Philip W. Hammond, Ezra S. Abrams, T. Christian Boles, Andrew R. Muir