Patents Examined by Eggerton Campbell
  • Detectably labeled, dual conformation oligonucleotide probes, assays and kits
    Patent number: 6103476
    Abstract: Unimolecular and bimolecular hybridization probes for the detection of nucleic acid target sequences comprise a target complement sequence, an affinity pair holding the probe in a closed conformation in the absence of target sequence, and either a label pair that interacts when the probe is in the closed conformation or, for certain unimolecular probes, a non-interactive label. Hybridization of the target and target complement sequences shifts the probe to an open conformation. The shift is detectable due to reduced interaction of the label pair or by detecting a signal from a non-interactive label. Certain unimolecular probes can discriminate between target and non-target sequences differing by as little as one nucleotide. Also, universal stems and kits useful for constructing said probes. Also, assays utilizing said probes and kits for performing such assays.
    Type: Grant
    Filed: March 15, 1999
    Date of Patent: August 15, 2000
    Assignee: The Public Health Research Institute of the City of New York, Inc.
    Inventors: Sanjay Tyagi, Fred R. Kramer, Paul M. Lizardi
  • Methods and reagents for typing HLA class I genes
    Patent number: 6103465
    Abstract: A method for typing HLA class 1 genes. The method entails first contacting a sample DNA with first and second amplification primers, wherein the first and second primers are each at least partially located in an exonic region. Next, using the first and second primers, a target sequence is amplified by the PCR to form an amplicon of the target sequence. Finally, the amplicon is detected with a sequence-specific detection means, e.g., DNA sequencing. The invention also includes specific amplification primers, specific sequencing primers, and kits especially adapted for use with the above HLA typing method.
    Type: Grant
    Filed: October 3, 1995
    Date of Patent: August 15, 2000
    Assignee: The Perkin-Elmer Corporation
    Inventors: Leslie Johnston-Dow, Robert B. Chadwick, Peter Parham
  • Methods and compositions for identifying novel secreted mammalian polypeptides in yeast
    Patent number: 6103472
    Abstract: Method for identifying novel secreted mammalian proteins in yeast are described. Reporter polypeptides which allow detection of signal sequences by growth selection are also described.
    Type: Grant
    Filed: February 20, 1998
    Date of Patent: August 15, 2000
    Assignee: Amgen Inc.
    Inventor: Sushil K. Thukral
  • Plasmid for delivery of nucleic acids to cells and methods of use
    Patent number: 6103470
    Abstract: This invention relates to plasmid constructs useful for nonviral human gene therapy. The plasmid constructs incorporate plasmid elements for achieving high copy number, avoiding plasmid instability an providing a plasmid selection process. These constructs can be used to deliver nucleic acids to cells in vehicles which are compatible with human therapeutic use.
    Type: Grant
    Filed: December 31, 1997
    Date of Patent: August 15, 2000
    Assignee: Genemedicine, Inc.
    Inventors: Eric M. Eastman, Ross H. Durland
  • Use of selective DNA fragment amplification products for hybridization-based genetic fingerprinting, marker assisted selection, and high-throughput screening
    Patent number: 6100030
    Abstract: Methods of genotyping amplified mixtures of DNAs, nucleic acid markers and methods of obtaining markers, kits, recombinant plants, positional cloning and integrated systems for making genotypes and assessing hybridizations are provided. These features are applicable to DNA fingerprinting, marker assisted selection, genotyping, cladistic analysis of variance, and high throughput laboratory screening methods.
    Type: Grant
    Filed: January 9, 1998
    Date of Patent: August 8, 2000
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Rhonda J. McCasky Feazel, Timothy G. Helentjaris, Sharon E. Malmberg, Barry A. Martin
  • MTS1 gene
    Patent number: 6090578
    Abstract: The present invention relates to somatic mutations in the Multiple Tumor Suppressor (MTS) gene in human cancers and their use in the diagnosis and prognosis of human cancer. The invention further relates to germ line mutations in the MTS gene and their use in the diagnosis of predisposition to melanoma, leukemia, astrocytoma, glioblastoma, lymphoma, glioma, Hodgkin's lymphoma, CLL, and cancers of the pancreas, breast, thyroid, ovary, uterus, testis, kidney, stomach and rectum. The invention also relates to the therapy of human cancers which have a mutation in the MTS gene, including gene therapy, protein replacement therapy and protein mimetics. Finally, the invention relates to the screening of drugs for cancer therapy.
    Type: Grant
    Filed: December 8, 1997
    Date of Patent: July 18, 2000
    Assignee: Myriad Genetics, Inc.
    Inventor: Alexander Kamb
  • Method for analyzing DNA from a rare cell in a cell population
    Patent number: 6087134
    Abstract: Methods are provided for analyzing DNA of a rare cell in a cell population. In one embodiment, the method involves covering a cell monolayer with a photosensitive material. By illuminating the area over a cell of interest, the material is solidified, permitting manipulation of the underlying cell and/or protection of the cell from DNA-inactivating agents that destroy DNA in other cells in the monolayer. In another embodiment, the monolayer is overlaid with a solid material that becomes soluble when illuminated. By illuminating the area over a cell of interest, that cell can be specifically exposed and DNA from the cell amplified. The methods are particularly useful for analyzing fetal cells found in maternal blood.
    Type: Grant
    Filed: October 23, 1997
    Date of Patent: July 11, 2000
    Assignee: Applied Imaging Corporation
    Inventor: Alexander Michael Saunders
  • Recombinant expression of proteins from secretory cell lines
    Patent number: 6087129
    Abstract: The present invention a provides methods for production of heterologous polypeptides using a variety recombinantly engineered secretory cell lines. The common feature of these cell lines is the absence of expression of at least one endogenous polypeptide. The host cell machinery normally used to produce the endogenous polypeptide is then usurped for the purpose of making the heterologous polypeptide. Also described are methods engineering cells for high level expression, methods of large scale protein production, and methods for treatment of disease in vivo using viral delivery systems and recombinant cell lines.
    Type: Grant
    Filed: January 19, 1996
    Date of Patent: July 11, 2000
    Assignees: Betagene, Inc., Board of Regents, The University of Texas System
    Inventors: Christopher B. Newgard, Karl D. Normington, Samuel A. Clark, Anice E. Thigpen, Christian Quaade, Fred Kruse
  • Nucleic acids conferring and inducible resistance to glycopeptides particularly in gram-positive bacteria
    Patent number: 6087106
    Abstract: The invention relates to a protein VanB involved, in Gram-positive bacteria, in resistance to glycopeptides, particularly to vancomycine, said resistance being of the type inducible by the vancomycine and non-inducible by teicoplanine. The invention also relates to the utilisation of fragments of nucleotides of the gene van B for the detection of resistances to glycopeptides.
    Type: Grant
    Filed: April 22, 1998
    Date of Patent: July 11, 2000
    Assignee: Institut Pasteur
    Inventors: Michel Arthur, Sylvie Dutka-Malen, Stefan Evers, Patrice Courvalin
  • Receptor identification method
    Patent number: 6077666
    Abstract: The present invention relates to a method for isolating and cloning receptor DNA sequences. The invention also provides novel DNA sequences encoding a novel somatostatin receptor subtype.
    Type: Grant
    Filed: May 8, 1997
    Date of Patent: June 20, 2000
    Assignee: American Cyanamid Company
    Inventors: John Richard Hadcock, Bradley Alton Ozenberger, Mark Henry Pausch
  • Amplification and detection of Campylobacter jejuni and Campylobacter coli
    Patent number: 6066461
    Abstract: Amplification primers and methods for specific amplification and detection of a Campylobacter jejuni and C. coli target are disclosed. The primer-target binding sequences are useful for amplification and detection of C jejuni and C. coli target in a variety of amplification and detection reactions.
    Type: Grant
    Filed: April 12, 1999
    Date of Patent: May 23, 2000
    Assignee: Becton Dickinson and Company
    Inventors: Ray A. McMillian, Thomas L. Fort, Qimin You
  • Global amplification of nucleic acids
    Patent number: 6066457
    Abstract: Nucleic acid starting material composed of a collection of single stranded nucleic acid molecules is anchored and processed by a direction random printing method to produce a mixture of shorter random size DNA molecules well-suited for achieving substantially uniform global PCR amplification. The processing and global amplification method disclosed is especially useful in conjunction with subtractive hybridization procedures applied, for example, to the study of differential gene expression.
    Type: Grant
    Filed: April 29, 1998
    Date of Patent: May 23, 2000
    Assignee: Cancer Research Campaign Technology Limited
    Inventors: Ian Noel Hampson, Lynne Hampson
  • Alignment mechanism
    Patent number: 6063579
    Abstract: The invention is a method for improving material transfer manipulations in an automated format. The method entails deforming the work surface of a plate to flatten or elongate the work surface prior to or simultaneously to material transfer.
    Type: Grant
    Filed: October 30, 1998
    Date of Patent: May 16, 2000
    Assignee: Incyte Pharmaceuticals, Inc.
    Inventors: Joeben Bevirt, Gabriel Brinton, Eric Lachenmeier
  • Primers for obtaining highly informative DNA markers
    Patent number: 6060243
    Abstract: Disclosed is a DNA amplification primer pair for the amplification of Sextolet 900 marker, which comprises R14B/264 primer having the sequence of SEQ ID NO:1 and a primer designed from the nucleic acid sequence of Sextolet 900 marker (SEQ ID NO:2). A method for the DNA fingerprinting identification of genetically related or unrelated individuals, which comprises the steps of performing DNA amplification of genomic DNA samples collected from individuals, using the primer pair R14B/264 and Sext.900E (SEQ ID NO:1 and SEQ ID NO:3, respectively) and separating the amplified DNA segments obtained whereby Sextolet 900 marker, having a heterozigosity of at least 0.97 and comprising more than 64 alleles, is amplified and serves as DNA fingerprinting of the individuals.
    Type: Grant
    Filed: July 17, 1997
    Date of Patent: May 9, 2000
    Assignee: Procrea BioSciences Inc.
    Inventors: Jian Qing Tang, Serge B. Melancon
  • Amplification and detection of shigella spp. and enteroinvasive strains of Escherichia coli
    Patent number: 6060252
    Abstract: Amplification primers and methods for specific amplification and detection of a Shigella spp. and enteroinvasive strains of Escherichia coli (EIEC) target are disclosed. The primer-target binding sequences are useful for amplification and detection of Shigella and EIEC target in a variety of amplification and detection reactions.
    Type: Grant
    Filed: April 12, 1999
    Date of Patent: May 9, 2000
    Assignee: Becton Dickinson and Company
    Inventors: Tobin J. Hellyer, Ray A. McMillian
  • Identification and comparison of protein-protein interactions that occur in populations and identification of inhibitors of these interactors
    Patent number: 6057101
    Abstract: Methods are described for detecting protein-protein interactions, among two populations of proteins, each having a complexity of at least 1,000. For example, proteins are fused either to the DNA-binding domain of a transcriptional activator or to the activation domain of a transcriptional activator. Two yeast strains, of the opposite mating type and carrying one type each of the fusion proteins are mated together. Productive interactions between the two halves due to protein-protein interactions lead to the reconstitution of the transcriptional activator, which in turn leads to the activation of a reporter gene containing a binding site for the DNA-binding domain. This analysis can be carried out for two or more populations of proteins.
    Type: Grant
    Filed: June 13, 1997
    Date of Patent: May 2, 2000
    Assignee: CuraGen Corporation
    Inventors: Krishnan Nandabalan, Jonathan Marc Rothberg, Meijia Yang, James Robert Knight, Theodore Samuel Kalbfleisch
  • Oligonucleotide arrays
    Patent number: 6057100
    Abstract: The invention relates to novel oligonucleotide arrays.
    Type: Grant
    Filed: June 6, 1997
    Date of Patent: May 2, 2000
    Assignee: EOS Biotechnology, Inc.
    Inventor: Herbert L. Heyneker
  • Dyes designed for high sensitivity detection of double-stranded DNA
    Patent number: 6054272
    Abstract: Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
    Type: Grant
    Filed: November 7, 1997
    Date of Patent: April 25, 2000
    Assignee: The Regents of the University of California
    Inventors: Alexander N. Glazer, Scott C. Benson
  • Method for screening for enzyme activity
    Patent number: 6054267
    Abstract: Disclosed is a process for identifying clones having a specified enzyme activity by screening for the specified enzyme activity in a library of clones prepared by (i) selectively isolating target DNA from DNA derived from at least one microorganism, by use of at least one probe DNA comprising at least a portion of a DNA sequence encoding an enzyme having the specified enzyme activity; and (ii) transforming a host with isolated target DNA to produce a library of clones which are screened for the specified enzyme activity.
    Type: Grant
    Filed: August 2, 1996
    Date of Patent: April 25, 2000
    Assignee: Diversa Corporation
    Inventor: Jay M. Short
  • Multiplex assay for nucleic acids employing transponders
    Patent number: 6051377
    Abstract: Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. A dedicated read/write device is used to remotely encode or read the data.
    Type: Grant
    Filed: December 9, 1996
    Date of Patent: April 18, 2000
    Assignee: Pharmaseq, Inc.
    Inventor: Wlodek Mandecki