Abstract: It is disclosed a process for producing a bio-product from a ligno-cellulosic biomass, wherein the ligno-cellulosic biomass, a gas and steam are introduced in a pressurized reactor operated in a continuous manner at conditions to have a vapor head space. In the pressurized reactor, the vapor head space is maintained at a vapor head space temperature and at a pressure which is at least 1 bar greater than a pure steam equilibrium pressure at the vapor head space temperature. The ligno-cellulosic biomass is then refined by rapidly releasing the pressure while discharging the ligno-cellulosic biomass from the pressurized reactor assembly to create a pre-treated ligno-cellulosic biomass, which is then hydrolyzed and converted to the bio-product. Preferably, at least a portion of the gas is air entrapped in the ligno-cellulosic biomass and it is introduced into the pressurized reactor as part of the ligno-cellulosic biomass.

Abstract: Compositions including metal nano- and/or micro-particles in solution with photosynthetic bioproduct producing microorganisms. These light harvesting complexes increase growth rates and photosynthetic efficiency of the constituent microorganisms, reducing the light required for a specific production level, or increases production for a specific light level.

Abstract: The present invention is related a method of purifying a heterologous protein from an egg white. In some embodiments, the disclosure is directed to a method of purifying a heterologous protein from an egg white comprising the heterologous protein, the method comprising, (a) adjusting the pH of the egg white to a pH of 5.8 to 6.5 to form a pH-adjusted egg white; (b) filtering the pH-adjusted egg white of (a) and collecting a first filtrate; (c) subjecting the first filtrate of (b) to a hydrophobic interaction chromatography matrix, and collecting a first eluate comprising the heterologous protein; (d) adjusting the pH of the first eluate of (c) to a pH of 5.0 to 5.6 to form a pH-adjusted eluate; (e) filtering the pH-adjusted eluate to obtain a second filtrate; (f) adjusting the pH of the second filtrate to a pH of 3.0 to 4.0 to form a pH-adjusted second filtrate; (g) neutralizing the pH-adjusted second filtrate of (f) to a pH of 5.0 to 8.

Abstract: Methods and apparatuses to non-destructively and periodically sample a small quantity of intracellular proteins and mRNA from the same single cell or cells for an extended period of time. Specifically, describe herein are non-perturbative methods for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and nucleic acids from a variety of cell types using systems including nanostraws.

Abstract: A three dimensional cell culture and bioreactor system is provided. The system comprises one or more cell culture chamber. Each cell culture chamber comprises an inlet port and an outlet port in fluid communication with the cell culture chamber. The cell culture chambers may be segregated or in fluid communication with one another. The systems may be used to conduct drug efficacy test, isolate certain cell types from a complex tissue sample of multiple cell types, allow for the ex vivo culturing of patient tissue samples to help guide the course of treatment, and conduct co-culture experiments.

Abstract: A biomarker panel including a four-panel test for clotting that detects soluble fibrin (SF), thrombin-antithrombin complex (TAT), antithrombin III (ATIII), and plasminogen activator inhibitor (PAI-1). A biomarker panel including a three-panel test for glycocalyx integrity that detects syndecan-1 (SDC1), heparan sulfate (HS), and hyaluronidase (HAD). A biomarker panel including a test that detects a biomarker chosen from soluble fibrin (SF), thrombin-antithrombin complex (TAT), antithrombin III (ATIII), plasminogen activator inhibitor (PAI-1), syndecan-1 (SDC1), heparan sulfate (HS), hyaluronidase (HAD), and combinations thereof. A kit including a biomarker panel, instructions for use, materials to take and apply samples to the panel, and descriptions of biomarker levels and their meaning. Methods of detecting the presence of vascular disease, determining the stage of vascular disease, monitoring the progress of vascular disease treatments, and monitoring the efficacy of drugs during drug development.

Abstract: A microorganism culture sheet is provided which can limit the use of a costly cultivation material to a necessary portion, which has simple production at the time of manufacture, and can be simply and stably used by a user. The microorganism culture sheet of the present invention is a microorganism culture sheet comprising a base sheet, a culture layer formed on top of the base sheet, and a cover sheet that covers the culture layer, and the culture layer is pattern-formed with an application liquid comprising a polyvinylpyrrolidone and at least on selected from the group consisting of a gelling agent, a nutrient component, a color indicator, a selective agent and a substrate.

Abstract: The present invention concerns methods of using bioactive renal cell populations to provide regenerative effects to a native kidney for the treatment of chronic kidney disease.

Abstract: Provided are a functional microbial material and a method for producing a fermentation product, wherein the functional microbial material and the fermentation product are environment-friendly and improve the quality of crops. The present invention relates to a microbial material and a method for producing a fermentation product wherein the microbial material and the fermentation product are obtained by fermentation of a fermentation raw material including a plant-derived raw material and an animal-derived raw material using a population of microorganisms including a plurality of species of thermophilic microorganisms, and lead to a remedy for soil and water pollution and to the inhibition of greenhouse gas generation, as well as contribute to improvements in the functionality of plants, particularly the expression of genes involved in biophylaxis and resistant to high-temperature disorder, and an increase in antioxidant components.

Abstract: A post-surgical healing accelerator (PSHA) device for tissue and nerve repair at an injury site. The device includes (a) a substrate, (b) a scaffold disposed on a surface of the substrate, and (c) a population of cells attached to the scaffold. The scaffold comprises at least one modified polymer selected from a modified collagen, a modified gelatin, a modified alginate, a modified cellulose, a modified hyaluronic acid, and others, with (i) modifications configured to increase an interaction between the scaffold and the cells, (ii) modifications configured to increase an association of the at least one modified polymer with the substrate, and (iii) a combination of (i) and (ii). The cells attached to the scaffold are configured to carry out tissue and/or nerve repair at an injury site of a subject through at least one of growth, differentiation, and migration following an application of the device to the injury site of the subject.

Abstract: The disclosure features methods for treating hypophosphatasia (HPP) in a patient (e.g., a child having HPP) exhibiting physical impairments, disability in activities of daily living (ADL), pain, and/or delayed motor development by administering a soluble alkaline phosphatase (sALP) to the patient.

Abstract: Use of activated protein C (APC) for treatment and prevention of ocular diseases, disorders or conditions associated with retinal leakage and CNV such as age-related macular degeneration, optionally in combined therapy with anti-angiogenesis, anti-inflammatory, immunosuppressive, and anti PDGF agent.

Abstract: Cartilage fibers and implants made therefrom are disclosed, with and without cartilage particles. Methods for making the cartilage fibers and the implants containing them are also disclosed. The implants may be pre-shaped and may be reshapable and provide good shape retention and little swelling when placed into a cartilage defect.

Abstract: Brain microvascular endothelial cells (BMECS) can be generated from pluripotent stem cells, and possess membrane barrier functions along with capability for maturation into other developing tissues. This cell type has not been successfully frozen with loss of significant viability and/or BMEC functional properties. For example, BMECs can be used to model barrier function in blood brain barrier, by calculating the trans-endothelial resistance (TEER). However, thawed primary BMECs lose TEER resistance. By optimizing cell preparation, freezing media selection, and the controlled freezing, the Inventors have achieved complete recovery of frozen cells, achieving proper tight junction protein expression and physiologically relevant TEER. The freezing methods and compositions described herein, thereby allow for BMECs to be manufactured, frozen and distributed at scale.

Abstract: Provided herein according to some embodiments is a biocompatible scaffold comprising stem cells (e.g., a pliable scaffold) suitable for intracavity administration after surgical removal of a tumor, wherein the scaffold allows the stem cells to migrate away from the scaffold and towards a cancerous or damaged tissue, wherein the stem cells are loaded with a therapeutic agent and/or a reporter molecule. Methods of forming the scaffold, and methods of use thereof, are also provided.

Abstract: The invention relates to enzyme containing water-soluble films, and their use in detergents.

Abstract: The invention relates to a novel method for producing a stable precipitate enriched in phycobiliproteins by means of salicylic acid precipitation. The invention also relates to the use of said precipitate enriched in phycobiliproteins for producing cosmetic or dermatological, and food or nutraceutical compositions.

Abstract: The purpose of the present invention is to provide a novel medicinal use in regeneration medicine, said medicinal use comprising using pluripotent stem cells (Muse cells). Provided is a cell preparation for treating skin ulcer, said cell preparation comprising SSEA-3 positive pluripotent stem cells isolated from a mesenchymal tissue of a living organism or cultured mesenchymal cells. The cell preparation according to the present invention is based on such mechanism of skin tissue regeneration that, when the Muse cells are administered to a skin ulcer site of a subject suffering from the aforesaid disease, the Muse cells differentiate into skin-constituting cells.

Abstract: Methods and compositions comprising carotenoids for the treatment or prevention of nausea, e.g., chemotherapy-induced nausea and vomiting.

Abstract: The present invention is related to the field of bioprotection, in particular to an antimicrobial composition comprising a Lactobacillus rhamnosus strain and/or a Lactobacillus paracasei strain. Furthermore, the present invention concerns uses for such an antimicrobial composition, food, feed and pharmaceutical products comprising such an antimicrobial composition, a method of manufacturing such food, feed and pharmaceutical products and a method for reducing the content of unwanted microorganisms of such food, feed and pharmaceutical products.