Abstract: The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.
Type:
Grant
Filed:
May 7, 2021
Date of Patent:
January 31, 2023
Assignees:
Massachusetts Institute of Technology, National University of Singapore
Inventors:
Parayil K. Ajikumar, Gregory Stephanopoulos, Heng Phon Too
Abstract: The present invention relates to the identification of a genomic integration site for heterologous polynucleotides in Chinese Hamster Ovary (CHO) cells resulting in high RNA and/or protein production. More specifically it relates to CHO cells comprising at least one heterologous polynucleotide stably integrated into the S100A gene cluster of the CHO genome and to methods for the production of said CHO cells. Further, the invention relates to a method for the production of a protein of interest using said CHO cell and to the use of said CHO cell for producing a protein of interest at high yield. Integration within these specific target regions leads to reliable, stable and high yielding production of an RNA and/or protein of interest, encoded by the heterologous polynucleotide.
Type:
Grant
Filed:
August 10, 2018
Date of Patent:
January 24, 2023
Assignee:
Boehringer Ingelheim International GmbH
Inventors:
Markus Mueller, Jochen Schaub, Christian Bernloehr, Jennifer Koenitzer
Abstract: Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
Type:
Grant
Filed:
August 19, 2020
Date of Patent:
January 24, 2023
Assignee:
BASF SE
Inventors:
Brian Michael Green, Michelle Lorraine Gradley
Abstract: The present invention provides compositions of recombinant human lysosomal acid lipase having particular glycosylation patterns for internalization into target cells, a vector containing the nucleic acid encoding human lysosomal acid lipase, a host cell transformed with the vector, pharmaceutical compositions comprising the recombinant human lysosomal acid lipase and method of treating conditions associated with lysosomal acid lipase deficiency.
Abstract: The present invention relates to fusion proteins comprising (i) a fusion moiety based on SEQ ID NO:1 and (ii) a protein. Also provided are nucleic acids encoding such fusion proteins and compositions comprising such fusion proteins. The invention also provides a method for increasing the expression level of a protein in a host cell or increasing the level of secretion of a protein from a host cell, said methods employing a fusion moiety in accordance with the invention. The invention further provides a method of producing a protein, said method comprising culturing an Aliivibrio wodanis host cell comprising a heterologous nucleic acid molecule encoding a protein under conditions suitable for the expression of the encoded protein. Certain deposited strains of Aliivibrio wodanis are also provided.
Type:
Grant
Filed:
June 19, 2019
Date of Patent:
January 24, 2023
Assignee:
UNIVERSITETET I TROMSØ—NORGES ARKTISKE UNIVERSITET
Inventors:
Peik Haugen, Miriam Grgic, Jenny Johansson Söderberg
Abstract: Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.
Abstract: This invention provides an isomaltooligosaccharide (IMO) composite and the method to manufacture. According to this invention, isomaltooligosaccharide with a high level of sweetness can be provided without an additional process of adding fructose.
Abstract: Disclosed herein include methods of reducing the viscosity of a starch slurry during starch processing by the addition of one or more surfactants and one or more amylases. In some embodiments, the unexpected reduction in slurry viscosity results in a surprising increase in corn oil recovery, reduction in residual starch, and increased fermentation rate.
Type:
Grant
Filed:
May 23, 2019
Date of Patent:
December 20, 2022
Assignees:
BASF SE, BASF Enzymes LLC
Inventors:
Yukiko Sato, Frank Reinhold, Justin Andrew Bordley, Joseph P Borst, Matthew Gerard Lyon, Christopher Paul Myers, Katrina Marie Knauer
Abstract: Disclosed are peptides and dimeric peptides exhibiting beta-galactosidase enzyme activity, as well as methods for producing a dairy product using them, and dairy products made by such methods. In some embodiments, the peptides exhibit beta-galactosidase enzyme activity at low and/or high temperatures.
Type:
Grant
Filed:
April 11, 2018
Date of Patent:
December 13, 2022
Assignee:
Chr. Hansen A/S
Inventors:
Hans Raj, Pernille Smith, Thomas Eckhardt, Vojislav Vojinovic, Charlotte Elisabeth Grüner Schöller, Johannes Maarten Van Den Brink
Abstract: The present disclosure relates to recombinant prenyltransferase enzymes with increased thermostability and activity and the use of these enzymes in compositions and methods for biosynthesis involving prenylation reactions, including compositions and methods for the preparation of cannabinoids.
Abstract: The invention is directed to kits, compositions, tools and methods comprising a cyclic industrial process to form biocement. In particular, the invention is directed to materials and methods for decomposing calcium carbonate into calcium oxide and carbon dioxide at an elevated temperature, reacting calcium oxide with ammonium chloride to form calcium chloride, water, and ammonia gas; and reacting ammonia gas and carbon dioxide at high pressure to form urea and water, which are then utilized to form biocement. This cyclic process can be achieved by combining industrial processes with the resulting product as biocement. The process may involve retention of calcium carbonate currently utilized in the manufacture of Portland Cement.
Type:
Grant
Filed:
October 5, 2018
Date of Patent:
December 6, 2022
Assignee:
BIOMASON INC.
Inventors:
Ginger K. Dosier, John Michael Dosier, Kent J. Smith
Abstract: Glucoamylase mutant GA3 with improved specific activity and thermal stability, and its gene and application are provided, belonging to the field of gene engineering. The glucoamylase mutant GA3 of the present invention obtained by sequentially mutation at fixed points starting from wild glucoamylase processes both improved thermal stability and catalytic efficiency, and can be applied to the industries of feed, food, medicine and the like.
Type:
Grant
Filed:
February 6, 2021
Date of Patent:
November 29, 2022
Assignee:
Shandong Lonct Enzymes Co. LTD
Inventors:
Xingji Wang, Kefen Wang, Jie Zhang, Xinwei Tong, Wenlong Liu
Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.
Abstract: This present invention provides for a genetically modified host cell, or a cell-free reaction system, and related methods and materials for the biocatalytic production of an alkyl lactones from a hydroxy fatty acid, or natural alkyl lactones and esters from sugars using non-natural combinations of enzymes.
Type:
Grant
Filed:
March 5, 2020
Date of Patent:
November 22, 2022
Assignee:
The Regents of the University of California
Abstract: A method for producing a cellooligosaccharide that enables the formation of a cellooligosaccharide having a high degree of polymerization to be suppressed in enzymatic synthesis of a cellooligosaccharide, the method comprising reacting ?-glucose-1-phosphate and at least one primer selected from the group consisting of glucose, cellobiose, and alkylated glucose with cellodextrin phosphorylase in a mixed solvent containing water and a water-soluble organic solvent.
Type:
Grant
Filed:
April 11, 2019
Date of Patent:
November 22, 2022
Assignees:
DAI-ICHI KOGYO SEIYAKU CO., LTD., TOKYO INSTITUTE OF TECHNOLOGY
Abstract: The present invention relates to an enzyme having ?-1,6-glucosyl transfer activity, which can use a partially degraded starch product as a substrate and is heat resistant and suitable for industrial applications; an enzyme preparation for use in manufacturing ?-1,6-glucan, comprising the enzyme as an active ingredient; and a method for manufacturing ?-1,6-glucan using the enzyme or enzyme preparation. The present invention provides an enzyme having ?-1,6-glucosyl transfer activity, which is any one of proteins (a), (b), and (c): (a) a protein consisting of an amino acid sequence of SEQ ID NO: 3; (b) a protein consisting of an amino acid sequence having at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 3; and (c) a protein consisting of an amino acid sequence in which one or several amino acid(s) have been substituted, inserted, deleted and/or added in the amino acid sequence of SEQ ID NO: 3.
Abstract: The present invention discloses a method for establishing a colorectal cancer HK2 reporter gene cell line, specifically including: firstly, designing a site-specific sgRNA sequence of an HK2 gene, and cloning same into a PX459 plasmid; integrating a homologous recombination sequence of an HK2 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; and performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive HK2 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line HK2 gene and EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the HK2 gene.
Abstract: Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2) and increasing the activity of a type 2 diacylglycerol acyltransferase (i.e., DGA1). In some embodiments, the triacylglycerol content of a cell is also modified my decreasing the activity of a triacylglycerol lipase in the same cell. Also disclosed are methods and compositions for increasing the triacylglycerol content of a cell by increasing the activity of a type 1 diacylglycerol acyltransferase (i.e., DGA2), or by increasing the activity of a type 3 diacylglycerol acyltransferase (i.e., DGA3).
Type:
Grant
Filed:
April 5, 2019
Date of Patent:
November 8, 2022
Assignee:
Ginkgo Bioworks, Inc.
Inventors:
Vasiliki Tsakraklides, Elena E. Brevnova
Abstract: The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Grant
Filed:
April 26, 2018
Date of Patent:
November 8, 2022
Assignee:
Novozymes A/S
Inventors:
Vivek Srivastava, Markus Klinger, Rakhi Saikia, Vijaya Shankar Nataraj, Sohel Dalal, Jens Erik Nielsen, Rajendra Kulothungan Sainathan
Abstract: Methods of fractionating lignocellulosic biomass using maleic acids are provided. Also provided are methods of forming lignin particles, furans, sugars, and/or lignocellulosic micro- and nanofibrils from the liquid and solid fractions produced by fractionation process. The fractionation can be carried out at low temperatures with short reaction times to carboxylate and dissolve lignin with a low degree of condensation.