Patents Examined by Hope A. Robinson
  • Patent number: 10995323
    Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: December 17, 2019
    Date of Patent: May 4, 2021
    Assignee: Codexis, Inc.
    Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
  • Patent number: 10994068
    Abstract: The invention relates to methods and systems for removal of pathogens from blood or blood products. The invention further relates to methods and systems for treatment and diagnosis of infection in the blood and/or sepsis in a patient in need thereof.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: May 4, 2021
    Assignee: Stichting Sanquin Bloedvoorziening
    Inventors: Robin Van Bruggen, Astrid Elisabeth Visser
  • Patent number: 10995349
    Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: June 26, 2020
    Date of Patent: May 4, 2021
    Assignee: Codexis, Inc.
    Inventors: Haibin Chen, Yong Koy Bong, Fabien L. Cabirol, Anupam Gohel Prafulchandra, Tao Li, Jeffrey C. Moore, Martina Quintanar-Audelo, Yang Hong, Steven J. Collier, Derek Smith
  • Patent number: 10993446
    Abstract: Lipase enzymes and methods of using the lipases in a baking for improving the volume, stability, tolerance of a baked product and/or reducing and reducing or eliminating the use of DATEM.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: May 4, 2021
    Assignee: BASF SE
    Inventors: Cristina Pop, Adrienne Huston Davenport, Michael Liszka, Xuqiu Tan, Andreas Funke, Jochen Kutscher, Michael Seitter, Stefan Haefner
  • Patent number: 10988745
    Abstract: The invention provides for hybrid nuclease-albumin molecules with increased pharmacokinetic properties. The hybrid nuclease-albumin molecules of the invention have one or more nuclease domains (e.g., an RNase and/or DNase domain) operably coupled to an albumin, or a variant or fragment thereof. The invention also provides methods of treating or preventing a condition associated with an abnormal immune response.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: April 27, 2021
    Assignee: RESOLVE THERAPEUTICS, LLC
    Inventors: James Arthur Posada, Chris Gabel
  • Patent number: 10988785
    Abstract: The present invention is a method for the biosynthesis of hundreds of compounds, mainly found in the cannabis plant. The starting material for these compounds can be any biological compound that is used/produced in a biological organism from the sugar family starting materials or other low cost raw materials processed via enzymes or within organisms to give final products. These final products include, but are not limited to: cannabinoids, terpenoids, stilbenoids, flavonoids, phenolic amides, lignanamides, spermidine alkaloids, and phenylpropanoids. Specifically, the present invention relates to the regular, modified, or synthetic gene(s) of select enzymes that are processed and inserted into an expression system (for example, a vector, cosmid, BAC, YAC, phage) to produce modified hosts. The modified host is then optimized for efficient production and yield via manipulation, silencing, and amplifying inserted or other genes in the host, leading to an efficient system for product.
    Type: Grant
    Filed: August 27, 2019
    Date of Patent: April 27, 2021
    Assignee: CB THERAPEUTICS, INC.
    Inventors: Sher Ali Butt, Jacob Michael Vogan
  • Patent number: 10988744
    Abstract: The disclosure provides a method of producing recombinant alkaline phosphatase comprising: (i) culturing an alkaline phosphatase in a recombinant cell culture; (ii) obtaining a preparation of recombinant alkaline phosphatase from the cell culture; and (iii) minimizing in the preparation a concentration of at least one metal ion selected from the group consisting of: Nickel (Ni), Cobalt (Co), Copper (Cu), Manganese (Mn), Chromium (Cr), and Molybdenum (Mo).
    Type: Grant
    Filed: June 6, 2017
    Date of Patent: April 27, 2021
    Assignee: Alexion Pharmaceuticals, Inc.
    Inventors: Saravanamoorthy Rajendran, Rachael Alford, Sean Gallagher, Anne Kantardjieff, Jared Davis
  • Patent number: 10982243
    Abstract: The present invention is a method for the biosynthesis of hundreds of compounds, mainly found in the cannabis plant. The starting material for these compounds can be any biological compound that is used/produced in a biological organism from the sugar family starting materials or other low cost raw materials processed via enzymes or within organisms to give final products. These final products include, but are not limited to: cannabinoids, terpenoids, stilbenoids, flavonoids, phenolic amides, lignanamides, spermidine alkaloids, and phenylpropanoids. Specifically, the present invention relates to the regular, modified, or synthetic gene(s) of select enzymes that are processed and inserted into an expression system (for example, a vector, cosmid, BAC, YAC, phage) to produce modified hosts. The modified host is then optimized for efficient production and yield via manipulation, silencing, and amplifying inserted or other genes in the host, leading to an efficient system for product.
    Type: Grant
    Filed: August 28, 2019
    Date of Patent: April 20, 2021
    Assignee: CB THERAPEUTICS, INC.
    Inventors: Sher Ali Butt, Jacob Michael Vogan
  • Patent number: 10975405
    Abstract: The present invention relates to a method for chondroitin sulfate biosynthesis, belongs to the field of pharmaceuticals. CS was biosynthesized by sulfating the chondroitin with C4ST or C6ST in Tris-HCl buffer assisted with 3?-phosphoadenosine 5?-phophosulfate (PAPS). C4ST and C46ST came from bioengineered Escherichia coli or Pichia pastoris. Chondroitin came from bioengineered Bacillus subtilis 168.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: April 13, 2021
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Zhen Kang, Jian Chen, Guocheng Du, Zhengxiong Zhou
  • Patent number: 10960050
    Abstract: The present invention includes compositions and methods for treating diseases or disorders associated with pathological calcification or pathological ossification. In certain embodiments, the diseases or disorders are selected from the group consisting of Generalized Arterial Calcification of Infancy (GACI), Idiopathic Infantile Arterial Calcification (IIAC), Ossification of the Posterior Longitudinal Ligament (OPLL), hypophosphatemic rickets, osteoarthritis, calcification of atherosclerotic plaques, PXE, hereditary and non-hereditary forms of osteoarthritis, ankylosing spondylitis, hardening of the arteries occurring with aging, calciphylaxis resulting from end stage renal disease and progeria.
    Type: Grant
    Filed: March 25, 2020
    Date of Patent: March 30, 2021
    Assignee: Yale University
    Inventors: Demetrios Braddock, Ronald Albright
  • Patent number: 10961526
    Abstract: Provided is a microorganism that is able to efficiently produce protocatechuic acid or a salt thereof by using a saccharide as a raw material, and a method of efficiently producing protocatechuic acid or a salt thereof by using the microorganism. Provided is a transformant having protocatechuic acid producing ability, subjected to modifications of enhancement of 3-dehydroshikimate dehydratase activity; enhancement of chorismate pyruvate lyase activity; and enhancement of 4-hydroxybenzoate hydroxylase activity. Also provided is a method of producing protocatechuic acid or a salt thereof, including the step of culturing the transformant in a reaction solution containing a saccharide so as to cause the transformant to produce protocatechuic acid or a salt thereof.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: March 30, 2021
    Assignees: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH, SUMITOMO BAKELITE CO., LTD.
    Inventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Takahisa Kogure
  • Patent number: 10960049
    Abstract: The present invention includes compositions and methods for treating diseases or disorders associated with pathological calcification or pathological ossification. In certain embodiments, the diseases or disorders are selected from the group consisting of Generalized Arterial Calcification of Infancy (GACI), Idiopathic Infantile Arterial Calcification (IIAC), Ossification of the Posterior Longitudinal Ligament (OPLL), hypophosphatemic rickets, osteoarthritis, calcification of atherosclerotic plaques, PXE, hereditary and non-hereditary forms of osteoarthritis, ankylosing spondylitis, hardening of the arteries occurring with aging, calciphylaxis resulting from end stage renal disease and progeria.
    Type: Grant
    Filed: November 14, 2017
    Date of Patent: March 30, 2021
    Assignee: Yale University
    Inventors: Demetrios Braddock, Ronald Albright
  • Patent number: 10954499
    Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: March 23, 2021
    Assignee: Novozymes A/S
    Inventors: Hanne Risbjerg Soerensen, Christel Thea Joergensen, Lars Hylling Christensen, Christian Isak Joergensen, Carsten Hoerslev Hansen, Lene Venke Kofod
  • Patent number: 10954502
    Abstract: Compositions and methods for regulating the blood coagulation pathway are disclosed. More particularly, the present disclosure relates to thrombin-thrombomodulin fusion proteins, vectors, host cells and methods for preparing the thrombin-thrombomodulin fusion proteins. The present disclosure further relates to methods for measuring protein C in plasma and kits for measuring protein C in plasma.
    Type: Grant
    Filed: April 28, 2016
    Date of Patent: March 23, 2021
    Assignee: Saint Louis University
    Inventors: Sergio Barranco-Medina, Nicola Pozzi, Enrico Di Cera
  • Patent number: 10947522
    Abstract: The present invention provides a fungus of the genus Rhizopus having high productivity of an organic acid. The present invention also provides a mutant of the genus Rhizopus with reduced pyruvate decarboxylase activity.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: March 16, 2021
    Assignee: Kao Corporation
    Inventor: Yuichi Tsuboi
  • Patent number: 10927355
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: February 23, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Patent number: 10900055
    Abstract: The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.
    Type: Grant
    Filed: June 10, 2019
    Date of Patent: January 26, 2021
    Assignee: Codexis, Inc.
    Inventors: Gjalt W. Huisman, Joyce Liu, Nikki Dellas, Chinping Chng, William Casey Hallows
  • Patent number: 10898551
    Abstract: Provided are soluble neutral active Hyaluronidase Glycoproteins (sHASEGP's), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. Sialated and pegylated forms of the sHASEGPs also are provided. Methods of treatment by administering sHASEGPs and modified forms thereof also are provided.
    Type: Grant
    Filed: April 2, 2019
    Date of Patent: January 26, 2021
    Assignee: Halozyme, Inc.
    Inventors: Louis H. Bookbinder, Anirban Kundu, Gregory I. Frost
  • Patent number: 10894810
    Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium able to secrete a heterologous protein and modified so that the activity of HrrSA system is reduced is cultured to produce the heterologous protein by secretory production.
    Type: Grant
    Filed: April 19, 2019
    Date of Patent: January 19, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Yumi Ito, Yoshihiko Matsuda
  • Patent number: 10894809
    Abstract: A novel technique for improving secretory production of a heterologous protein by coryneform bacteria is described, and thereby a method for secretory production of a heterologous protein is provided. A coryneform bacterium able to secrete a heterologous protein modified so that the activity of RegX3 protein is reduced is cultured to produce the heterologous protein by secretory production.
    Type: Grant
    Filed: April 19, 2019
    Date of Patent: January 19, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Yumi Ito, Yoshihiko Matsuda