Patents Examined by Hope A. Robinson
  • Patent number: 10370688
    Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: August 6, 2019
    Assignee: Codexis, Inc.
    Inventors: Haibin Chen, Yong Koy Bong, Fabien L. Cabirol, Anupam Gohel Prafulchandra, Tao Li, Jeffrey C. Moore, Martina Quintanar-Audelo, Yang Hong, Steven J. Collier, Derek Smith
  • Patent number: 10358666
    Abstract: The present invention provides a method of biomass production using a modified alga having suppressed expression of ATG8.
    Type: Grant
    Filed: May 26, 2015
    Date of Patent: July 23, 2019
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Ken'ichi Ogawa, Masanobu Nishikawa, Kazuya Kiyokawa
  • Patent number: 10351816
    Abstract: The present invention relates to application of a novel signal peptide in L-arginine and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The present invention fused the signal peptide set forth in SEQ ID NO.1 with the ?-mannanase of Bacillus subtilis CCTCC M 209200, and expressed the fused gene in the strain with high L-arginine yield. The recombinant strain Corynebacterium crenatum CGMCC 0890/p MSPman had advantages on utilizing cheaper konjac powder as substrate, and after fermenting for 96 hours in a 5 L bioreactor, the L-arginine yield reached 45 g/L. Another two recombinant strains were constructed based on Corynebacterium crenatum CGMCC 0890/pMSPman, and after fermenting for 96 hours in a 5 L bioreactor, the L-ornithine yield and L-citrulline reached 23.5 g/L and 26.3 g/L respectively.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: July 16, 2019
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Zhiming Rao, Zhenghong Xu, Junxian Zheng, Meijuan Xu, Taowei Yang, Xian Zhang
  • Patent number: 10351859
    Abstract: The present disclosure relates to a microorganism producing L-leucine and a method for producing L-leucine using the same, and more specifically, to a Corynebacterium glutamicum mutant which has resistance to L-leucine and a derivative thereof and improved L-leucine producing ability, and a method for producing L-leucine using the same.
    Type: Grant
    Filed: August 25, 2016
    Date of Patent: July 16, 2019
    Assignee: CJ Cheiljedang Corporation
    Inventors: Byeong Cheol Song, Ji Hye Lee, Ae Ji Jeon, Jong Hyun Kim, Hye Won Kim
  • Patent number: 10350301
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a blood coagulation protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer. In one embodiment of the invention the conjugation is carried out in the presence of the nucleophilic catalyst aniline. In addition the generated oxime linkage can be stabilized by reduction with NaCNBH3 to form an alkoxyamine linkage.
    Type: Grant
    Filed: August 29, 2016
    Date of Patent: July 16, 2019
    Assignees: Baxalta Incorporated, Baxalta GmbH
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • Patent number: 10351886
    Abstract: Facile methods for high-yield furfural and HMF production from biomass sugars are described. The methods generally involve converting the biomass sugars in high yield to their ketose isomers, resulting in furan production under low temperature and pressure conditions with efficient recycling of the process streams.
    Type: Grant
    Filed: November 27, 2017
    Date of Patent: July 16, 2019
    Assignee: The University of Toledo
    Inventors: Siamak Alipour, Bin Li, Sasidhar Varanasi, Patricia Relue, Sridhar Viamajala
  • Patent number: 10336795
    Abstract: The invention discloses microorganism cell culture conditions that result in increased cellular and media concentrations of a biological pigment. The invention has applications in use as a natural food coloring, as antioxidants in the food supplement industries, in the nutraceutical, pharmaceutical, and cosmeceutical industries, and a non-toxic ink. The method results in pigment that is relatively easy to separate from the microorganism culture.
    Type: Grant
    Filed: February 6, 2018
    Date of Patent: July 2, 2019
    Assignee: University of Newcastle
    Inventors: Chelsea Marie Brain, Gary Stephen Caldwell
  • Patent number: 10329592
    Abstract: The present invention relates to application of a novel signal peptide in L-glutamate and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The signal peptide which mediated secretion of ?-mannanase was invented, and the recombinant strain with this signal peptide had advantages on utilizing konjac powder to produce related products, and its utilization efficiency of konjac powder, production efficiency, and yield were higher than other signal peptides. The recombinant strain possessing this new signal peptide had advantages on utilizing cheaper konjac powder as substrate to lower the process costs on L-glutamic acid and its high-value-added products.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: June 25, 2019
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Zhiming Rao, Junxian Zheng, Meijuan Xu, Taowei Yang, Xian Zhang
  • Patent number: 10329591
    Abstract: The present invention is related to a recombinant microorganism optimized for the fermentative production of methionine and/or its derivatives, wherein in said recombinant strain, the methionine efflux is enhanced by overexpressing the homologous logous genes of ygaZ and ygaH genes from Escherichia coli. It is also related to a method for optimizing the fermentative production of methionine or its derivatives comprising the steps of: a. culturing a recombinant microorganism wherein in said microorganism, the methionine efflux is enhanced by overexpressing the ygaZH homologous genes of ygaZ and ygaH genes from Escherichia coli, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and b. recovering methionine and/or its derivatives from the culture medium.
    Type: Grant
    Filed: August 31, 2015
    Date of Patent: June 25, 2019
    Assignee: Evonik Degussa GmbH
    Inventors: Rainer Figge, Laurence Dumon-Seignovert, Perrine Vasseur, Wanda Dischert
  • Patent number: 10323233
    Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: March 6, 2018
    Date of Patent: June 18, 2019
    Assignee: Codexis, Inc.
    Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
  • Patent number: 10323234
    Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: June 26, 2018
    Date of Patent: June 18, 2019
    Assignee: Codexis, inc.
    Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
  • Patent number: 10316297
    Abstract: The present disclosure relates to an acetohydroxy acid synthase variant in which the feedback inhibition to L-valine is released, a polynucleotide encoding the acetohydroxy acid synthase variant, an expression vector including the polynucleotide, a microorganism producing L-valine including the acetohydroxy acid synthase variant, and a method for producing L-valine using the microorganism.
    Type: Grant
    Filed: August 5, 2015
    Date of Patent: June 11, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Ae Ji Jeon, Byeong Cheol Song, Jong Hyun Kim, Hye Won Kim, Ji Hye Lee
  • Patent number: 10316298
    Abstract: Methods and compositions for producing fatty acid derivatives, for example, fatty esters, and commercial fuel compositions comprising fatty acid derivatives are described.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: June 11, 2019
    Assignee: REG Life Sciences, LLC
    Inventor: Alfred Gaertner
  • Patent number: 10308924
    Abstract: The present invention relates to methods for the manufacture, purification, formulation, and use of biologically active recombinant elastase proteins. Described are recombinant methods for producing therapeutically useful elastase proteins, as are pharmaceutical compositions comprising said elastase proteins. Novel recombinant elastase proteins and protein preparations are also disclosed. Methods are described for treating and preventing diseases of biological conduits using pharmaceutical compositions containing the elastase proteins of the invention.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: June 4, 2019
    Assignee: PROTEON THERAPEUTICS, INC.
    Inventors: F. Nicholas Franano, Kimberly Bland, Marco D. Wong, Bee C. Ding
  • Patent number: 10301663
    Abstract: Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions for use in the methods of the present invention, are provided.
    Type: Grant
    Filed: February 1, 2016
    Date of Patent: May 28, 2019
    Assignee: Wyeth, LLC
    Inventors: Wei-Qiang Willie Sun, Earl Joseph Pursell
  • Patent number: 10301638
    Abstract: The invention relates to a method for producing eicosapentaenoic acid, docosapentaenoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a ?6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a ?6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a ?5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a ?5 elongase activity, the nucleic acid sequence coding for a polypeptide with a ?5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a ?4 desaturase activity is also introduced into the plant.
    Type: Grant
    Filed: September 6, 2016
    Date of Patent: May 28, 2019
    Assignee: BASF Plant Science GmbH
    Inventors: Petra Cirpus, Jörg Bauer, Xiao Qiu, Guohai Wu, Bifang Cheng, Martin Truksa, Tom Wetjen
  • Patent number: 10301612
    Abstract: The present invention relates to methods for the manufacture, purification, formulation, and use of biologically active recombinant elastase proteins. Described are recombinant methods for producing therapeutically useful elastase proteins, as are pharmaceutical compositions comprising said elastase proteins. Novel recombinant elastase proteins and protein preparations are also disclosed. Methods are described for treating and preventing diseases of biological conduits using pharmaceutical compositions containing the elastase proteins of the invention.
    Type: Grant
    Filed: January 25, 2018
    Date of Patent: May 28, 2019
    Assignee: PROTEON THERAPEUTICS, INC.
    Inventors: F. Nicholas Franano, Kimberly Bland, Marco D. Wong, Bee C. Ding
  • Patent number: 10301608
    Abstract: Variants of phosphotriesterase have been created that exhibit enhanced hydrolysis of V-type and G-type nerve agents over wild-type phosphotriesterase. V- and G-type nerve agents have an SP and RP enantiomer. The SP enantiomers are more toxic. V-type nerve agents are among the most toxic substances known. Variants of phosphotriesterase can prefer to hydrolyze one enantiomer of VX over the other enantiomer.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: May 28, 2019
    Assignee: The Texas A&M University System
    Inventors: Frank M. Raushel, Andrew N. Bigley
  • Patent number: 10294467
    Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min. The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of P469A, P469C, P469D, P469E, P469F, P469G, P469H, P469I, P469K, P469L, P469M, P469N, P469Q, P469R, P469S, P469T, P469V, P469W, P469Y, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: May 21, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jan Metske Van Der Laan, Peter Jozef Ida Van De Vondervoort, Chantal Christis, Martine Spaans, Angela De Bruine-Paulus
  • Patent number: 10294463
    Abstract: A modified Bacillus cereus phospholipase C enzyme is provided, as well as a method of using the modified phospholipase C enzyme in a method of treating vegetable oil. In certain embodiments, this method may comprise combining a vegetable oil with an modified phospholipase C enzyme comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid residue at position 66 is a Trp (W) or Tyr (Y), and maintaining the combination under conditions suitable for the modified phospholipase C enzyme to catalyze the hydrolysis of phospholipids in the oil to produce diacylglycerol and a water soluble phosphate.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: May 21, 2019
    Inventors: Maria Eugenia Castelli, Hugo Menzella, Salvador Peiru, Leandro Vetcher