Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery.

Abstract: Disclosed is a gelatin-based nanoparticle complex for tumor-targeted delivery of siRNA for specific gene silencing in tumor cells. The gelatin-based nanoparticle complex includes: poly-siRNA chains whose ends are modified with thiol groups; and thiolated gelatin bound to the poly-siRNA chains through disulfide crosslinking and charge interactions. The gelatin-based nanoparticle complex is not degraded in the bloodstream and can be efficiently absorbed into tumor cells without cytotoxicity. The delivered siRNA can effectively silence target gene expression. Also disclosed is a method for preparing the gelatin-based nanoparticle complex.

Abstract: The present invention relates to a pesticide composition intended for protecting plants, crops or seeds against fungal diseases or insect damages, and the corresponding methods of protection by application of the said composition. More precisely, the subject of the present invention is a pesticide composition based on a tetrazolyloxime derivative and a thiazolylpiperidine derivative, which may further comprise another fungicide and/or an insecticide active substance or compound.

Abstract: Insecticide compositions which comprise one or not less than two kinds of compounds being selected from a compound represented by the formula [I]: or a salt thereof, and a neonicotinoid compound represented by the formula [II]: The insecticide compositions can produce higher insecticidal effects than would be expected when each of the active ingredients is used singly, namely a synergistic effect, thus enabling reductions in the rate or number of application of agrochemicals and pesticides to be realized.

Abstract: Compounds comprising an oligonucleotide moiety covalently linked to a lipid moiety are disclosed. The oligonucleotide moiety comprises a sequence that is complementary to the RNA component of human telomerase. The compounds inhibit telomerase activity in cells with a high potency and have superior cellular uptake characteristics.

Abstract: This disclosure provides methods to use nanoparticles as non-optical tags for detecting a change in mass. chemical sensing or bio-sensing events or reaction upon conjugation of nanoparticles onto a thermoresistor heat sensor. Particularly described is the use of metal nanoparticles in thermal sensors, thermal bio-sensors, and sensing pixel arrays for multiple analyte sensing. In addition, an asymmetric filter is disclosed that allows size separation of molecules from nanoparticles. The asymmetric filter is a porous membrane that is designed to have a small pore size in one size and a large pore size on the other side.

Abstract: The present invention is an organic composition for the protection of crops against black frost and the method for its application over the crops. The novelty of this invention consists not only in the composition of the product itself, but also in the calculation of the percentages of the organic components that intervene in the product; and, in the determination of the moment when the product must be applied over the crops. This invention is a very simple composition where the calculation of the percentages of the two main components is obtained by multiplying the “Filo Index” (real humidity and temperature/historical values of humidity and temperature for the area) by the component; and, the percentage of the second component is calculated by subtracting the first result from 100.

Abstract: The present invention relates to methods and compositions for the inhibition of gene expression. In particular, the present invention provides oligonucleotide-based therapeutics for the inhibition of oncogenes involved in cancers.

Abstract: Compounds comprising an oligonucleotide moiety covalently linked to a lipid moiety are disclosed. The oligonucleotide moiety comprises a sequence that is complementary to the RNA component of human telomerase. The compounds inhibit telomerase activity in cells with a high potency and have superior cellular uptake characteristics.

Abstract: Compounds comprising an oligonucleotide moiety covalently linked to a lipid moiety are disclosed. The oligonucleotide moiety comprises a sequence that is complementary to the RNA component of human telomerase. The compounds inhibit telomerase activity in cells with a high potency and have superior cellular uptake characteristics.

Abstract: A method of producing a nanoparticle assembly. The method includes attaching a first DNA molecule to a bead to form a first DNA-bead complex; and combining a nanoparticle with the first DNA-bead complex to form a nanoparticle-DNA-bead complex having one DNA molecule attached to the nanoparticle.

Abstract: The present invention relates generally to a population of cells genetically modified to produce insulin in a glucose responsive manner and uses thereof. More particularly, the present invention relates to a population of cells genetically modified to produce insulin in response to physiologically relevant levels of glucose and uses thereof. The cells of the present invention are useful in a wide variety of applications, in particular in the context of therapeutic and prophylactic regimes directed to the treatment of diabetes and/or the amelioration of symptoms associated with diabetes, based on the transplantation of the cells of the present invention into mammals requiring treatment. Also facilitated is the design of in vitro based screening systems for testing the therapeutic effectiveness and/or toxicity of potential adjunctive treatment regimes.

Abstract: The present invention relates to an aptamer comprising a nucleic acid comprising, or consisting of: —the sequence ACUGU CCCAG UAUGA CGCGA CUGCU UAGGU GGGAU GUUUC CCAUG CCUCG (SEQ ID NO: 1), or —a sequence comprising, or consisting of, at least 25 consecutive nucleotides in a sequence having at least 80% identity with SEQ ID NO: 1, with the proviso that a nucleic acid consisting of this sequence binds to the LAR protein.

Abstract: The present disclosure provides methods for treating neurological disorders, generally involving modulating protein kinase D1 (PKD1) activity levels in a neuron or glial cell in an individual in need thereof. The present disclosure provides antibodies specific for PKD1. The present disclosure provides a genetically modified non-human mammal deficient in PKD1 activity.

Abstract: The present invention relates to a magnetic nanoparticle for tumor therapy, comprising: a magnetic core; a shell encapsulating a surface of the magnetic core, wherein the shell is made of a polymer with carboxylic groups; a poly-nucleotide chain connected to a surface of the shell; an anti-tumor drug connected to the poly-nucleotide chain, wherein the anti-tumor drug comprises at least one functional group, and each of the functional group is independently a pyrimidine group or a purine group; and an antibody connected to the shell, wherein the antibody identifies a target tumor. In addition, the present invention further provides a method for manufacturing the magnetic nanoparticles for tumor therapy and a pharmaceutical composition containing the magnetic nanoparticles. Accordingly, the magnetic nanoparticle for tumor therapy of the present invention can achieve effective treatment of tumor by synergistic effects between hyperthermia and targeted chemotherapy.

Abstract: The invention relates to methods of preparing a bone matrix solution, a bone matrix implant, and variants thereof. The invention also relates to methods of cell culture using the same. The invention further relates to bone matrix scaffolds comprising one or more bone matrix nanofibers, methods of preparing, and methods of use thereof. The invention also relates to methods of culturing cells and promoting differentiation of stem cells using the same.

Abstract: A method of instantly reducing the appearance of wrinkles and skin imperfections while smoothing the skin, which comprises applying a cosmetic composition comprising a fractal particle based gel.

Abstract: The use of a bacterial secondary metabolite, thaxtomin is described as an effective herbicide on broadleaved, sedge and grass weeds, e.g., in rice fields with no phytotoxicity to rice as well as on aquatic based weeds.

Abstract: A composition is provided, comprising a non-ionic, water-soluble, hydrophobically modified lower alkyl hydroxyalkyl linear polysaccharide ether, wherein the hydrophobic group comprises a long-chain C10-C18 alkyl or alkenyl group, the lower alkyl group has 1-4 carbon atoms, and the hydroxyalkyl groups are hydroxyethyl and/or hydroxypropyl; and one or more agriculturally active ingredient. A method for treating a plant is also provided, comprising the step of contacting a plant with a composition of the invention.

Abstract: Compositions and processes for controlling nematodes are described herein, e.g., nematodes that infest plants or animals. The compounds include certain 2,5-substituted tetrazoles.