Patents Examined by Jezia Riley
  • Patent number: 10273261
    Abstract: Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a DNA polymerase complex so that polymerization may not be influenced.
    Type: Grant
    Filed: January 3, 2017
    Date of Patent: April 30, 2019
    Assignee: QUANTUMDX GROUP LIMITED
    Inventors: Jonathan J. O'Halloran, Joseph H. Hedley
  • Patent number: 10260094
    Abstract: This invention provides a process for sequencing nucleic acids using 3? modified deoxynucleotide analogs or 3? modified deoxyinosine triphosphate analogs, and 3? modified dideoxynucleotide analogs having a detectable marker attached to a base thereof.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: April 16, 2019
    Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Jingyue Ju, Dae Hyun Kim, Jia Guo, Qinglin Meng, Zengmin Li, Huanyan Cao
  • Patent number: 10253360
    Abstract: The invention provides a method of selectively amplifying a detection target nucleic acid by inhibiting amplification of a detection non-target nucleic acid (e.g., wild-type gene) in a test sample by using, in a nucleic acid amplification reaction, an oligonucleotide analog containing one or more kinds of one or more unit structures of various nucleoside analogs represented by the following formula (I): wherein the symbols are as defined in the DESCRIPTION, and the like, or a salt thereof, as a clamp nucleic acid, and detecting the amplified nucleic acid.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: April 9, 2019
    Assignee: BNA INC.
    Inventors: Takeshi Imanishi, Ayako Orita, Ikuya Ban
  • Patent number: 10246479
    Abstract: This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.
    Type: Grant
    Filed: April 8, 2013
    Date of Patent: April 2, 2019
    Assignees: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF COMMERCE
    Inventors: Jingyue Ju, Shiv Kumar, Chuanjuan Tao, Minchen Chien, James J. Russo, John J. Kasianowicz, Joseph W. F. Robertson
  • Patent number: 10246735
    Abstract: The present invention is in the field of sample preparation. In particular, it relates to methods for preparing samples prior to performing nucleic acid amplification.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: April 2, 2019
    Assignee: ROCHE MOLECULAR SYSTEMS, INC.
    Inventors: Clint Pereira, Cathal Joseph McElgunn, Laurence Carlo Tisi
  • Patent number: 10240178
    Abstract: Provided herein are mispriming prevention reagents, compositions and kits comprising such reagents and methods of use thereof.
    Type: Grant
    Filed: December 15, 2015
    Date of Patent: March 26, 2019
    Assignee: Brandeis University
    Inventors: Lawrence J. Wangh, Kenneth E. Pierce, Jesus A. Sanchez, John E. Rice, Alexandra Isabel King Over, Adam E. Osborne
  • Patent number: 10227582
    Abstract: The present disclosure relates to systems and methods for nucleic acid isolation from cellular samples. In particular, the present disclosure provides systems and methods for lysing cells and recovering nucleic acids.
    Type: Grant
    Filed: May 9, 2016
    Date of Patent: March 12, 2019
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Thomas N. Chiesl, Michelle Toro, Steven Gerald Haupt
  • Patent number: 10221449
    Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: March 5, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Linda Lee, Sam Woo, Peter Nien-Tung Ma
  • Patent number: 10221216
    Abstract: Described herein are recognition modules that bind specifically to a template nucleic acid and which ligate together in a reducing environment to produce a gamma peptide nucleic acid (?PNA) oligomer. Also provided are methods of synthesizing a ?PNA oligomer on a template using the recognition modules.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: March 5, 2019
    Assignee: Carnegie Mellon University
    Inventors: Danith H. Ly, Raman Bahal, Arunava Manna, Dinesh Chandra Bhunia, Rapireddy Srinivas, Yidan Cong
  • Patent number: 10214774
    Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: February 26, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Peter Williams, Daniel J. B. Williams, Linda Bloom, Thomas J. Taylor
  • Patent number: 10208341
    Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: February 19, 2019
    Assignee: Life Technologies Corporation
    Inventors: Peter Williams, Daniel J. B. Williams, Linda Bloom, Thomas J. Taylor
  • Patent number: 10202636
    Abstract: An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.
    Type: Grant
    Filed: December 24, 2013
    Date of Patent: February 12, 2019
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Bing Li, David Roger Moore, William Christopher Alberts, John Richard Nelson
  • Patent number: 10196418
    Abstract: Aspects of the present disclosure include compositions that make use of phosphorus and/or nucleobase protecting groups which find use in the synthesis of long polynucleotides. Phosphorus protecting groups are provided that help increase the stepwise coupling yield and/or phosphorous protecting groups that can be removed during the oxidation step. Amidine nucleobase protecting groups are provided that find use in the subject compositions and methods which provides for e.g., increased resistance to depurination during polynucleotide synthesis. In some instances, the methods and compositions disclosed herein utilize a combination of the phosphorus and amidine nucleobase protecting groups in the synthesis of polynucleotides having a sequence of 200 or more monomeric units in length. Also provided are methods for synthesizing a polynucleotide (e.g., a DNA) using one or more compounds disclosed herein.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: February 5, 2019
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Luca Monfregola, Marvin Caruthers, Mithun Roy
  • Patent number: 10196675
    Abstract: This invention relates to flat solid media for the storage of samples of biological materials and methods of analyzing biomolecules contained within the samples following storage. In particular, the invention relates to the storage and further analysis of biomolecules present in the biological materials, such as proteins, enzymes and nucleic acids. The invention finds particular utility in the dry, room temperature storage of biological materials.
    Type: Grant
    Filed: June 7, 2016
    Date of Patent: February 5, 2019
    Assignee: GE Healthcare UK Limited
    Inventors: Martin D. James, Jeffrey K. Horton, Peter J. Tatnell
  • Patent number: 10196673
    Abstract: Improved compositions for and methods of processing and analyzing samples are described. In particular, the compositions and methods liberate nucleic acids from a biological sample allowing direct downstream processing of the nucleic acids in microfluidic systems. These compositions, methods and kits are useful in diagnosing, staging or otherwise characterizing various biological conditions.
    Type: Grant
    Filed: February 18, 2014
    Date of Patent: February 5, 2019
    Assignee: BIOCARTIS N.V.
    Inventors: Koen Van Acker, Bart Claes, Benoit Devogelaere, Geert Maertens, Erwin Sablon, Pascale Holemans, Tania Ivens
  • Patent number: 10190162
    Abstract: Novel fluorescent nucleotide analogs are provided herein. Also provided herein are methods of using the nucleotide analogs in sequencing-by-synthesis and signal confinement methods.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: January 29, 2019
    Assignee: Complete Genomics, Inc.
    Inventors: Snezana Drmanac, Handong Li, Radoje Drmanac, Eric Harness, Chongjun Xu
  • Patent number: 10191060
    Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.
    Type: Grant
    Filed: November 9, 2010
    Date of Patent: January 29, 2019
    Assignees: University of Washington, Clemson Univeristy
    Inventors: Daniel T. Chiu, Changfeng Wu, Jason McNeill, Jiangbo Yu
  • Patent number: 10190157
    Abstract: Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.
    Type: Grant
    Filed: August 7, 2015
    Date of Patent: January 29, 2019
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaolin Wu, Xiaohai Liu
  • Patent number: 10184934
    Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.
    Type: Grant
    Filed: May 31, 2017
    Date of Patent: January 22, 2019
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
  • Patent number: 10184145
    Abstract: The present invention pertains to a method for isolating extracellular nucleic acids from a sample, wherein said sample is optionally stabilized, by binding the extracellular nucleic acids to a solid phase which carries anion exchange groups, comprising the following steps: binding the extracellular nucleic acids to the solid phase in a binding mixture having a first pH which allows binding the extracellular nucleic acids to the anion exchange groups of the solid phase; wherein the sample makes up at least 85% of the volume of the binding mixture; separating the solid phase with the bound extracellular nucleic acids; optionally washing the extracellular nucleic acids; optionally eluting extracellular nucleic acids from the solid phase. The method has the advantage that large sample volumes can be processed and that extracellular nucleic acids can be isolated rapidly with a high yield. The method is particularly suitable for automatable processes.
    Type: Grant
    Filed: September 25, 2012
    Date of Patent: January 22, 2019
    Assignee: QIAGEN GmbH
    Inventors: Martin Horlitz, Annette Nocon, Markus Sprenger-Haussels, Peter Grünefeld, Christoph Erbacher