Patents Examined by Jezia Riley
  • Patent number: 11680287
    Abstract: The invention relates to a method for the specific detection of a microorganism or a group of microorganisms via in situ hybridisation by means of flow cytometry.
    Type: Grant
    Filed: August 16, 2018
    Date of Patent: June 20, 2023
    Assignee: Vermicon AG
    Inventors: Jiri Snaidr, Claudia Beimfohr, Peter Muhlhahn
  • Patent number: 11667942
    Abstract: Processes described here exploit the utility of mismatching nucleobase analogs to create long segments of natural DNA to be constructed after fragments containing unnatural nucleotides are transliterated in at least two cycles of polymerase-catalyzed copying, or alternatively complete PCR, by guided mismatching.
    Type: Grant
    Filed: December 14, 2020
    Date of Patent: June 6, 2023
    Inventor: Steven A Benner
  • Patent number: 11667952
    Abstract: Provided are methods of producing product nucleic acids involving the use of oligonucleotides that are modified by the application of a stimulus. Aspects of such methods may include producing product nucleic acids using de-activatable oligonucleotides that are deactivated by a de-activating stimulus, as well as methods that may include producing product nucleic acids using activatable oligonucleotides that are activated by an activating stimulus and de-activatable oligonucleotides that are deactivated by a de-activating stimulus. Also provided are kits, compositions and devices that include de-activatable oligonucleotides or activatable and de-activatable oligonucleotides, e.g., for use in performing the methods as described herein.
    Type: Grant
    Filed: August 23, 2018
    Date of Patent: June 6, 2023
    Assignee: Takara Bio USA, Inc.
    Inventor: Kazuo Tori
  • Patent number: 11667943
    Abstract: Disclosed are PCR processes that transliterate target duplex DNA molecules containing 8 different nucleotide building blocks strategically arranged to allow efficient conversion of information in that target into DNA built from just four different nucleotides.
    Type: Grant
    Filed: January 22, 2021
    Date of Patent: June 6, 2023
    Inventor: Steven A Benner
  • Patent number: 11667961
    Abstract: The novel intercalating fluorescent compounds of exemplary embodiments of the present invention for analyzing nucleic acids, etc. have excellent intercalating efficiency with nucleic acids such as DNA and RNA of biomaterials, and may not only continuously maintain fluorescence properties and efficiency, but also have excellent effects even in terms of storage stability such as temperature and moisture, etc. and biosafety. In addition, the fluorescent compounds have various advantages capable of being dissolved in distilled water, which is a solvent harmless to the human body, and being applied to a wide range of analysis without being limited to the analysis of specific cells and living tissues.
    Type: Grant
    Filed: December 19, 2020
    Date of Patent: June 6, 2023
    Inventors: Jin Woo Park, Ho Young Si, Eunae Jeong, Hyung Jun Yoon, Su Jung Jang
  • Patent number: 11655503
    Abstract: Many regions of genomic DNA are highly similar to other regions of the genome and thus are very difficult to capture without also capturing the similar, undesired regions. This leads to over-sequencing of regions for which there is no interest and lowers coverage of the desired regions. To minimize the capture of non-desired regions, blocking baits have been designed to prevent similar but non-desired fragments from being captured. This allows more directed sequencing of the regions of interest. Blocking baits differ from capture baits in that they have modestly different sequence that preferentially bind the non-desired DNA and do not contain a biotin or other modification so remain behind when the capture baits are selected.
    Type: Grant
    Filed: May 10, 2021
    Date of Patent: May 23, 2023
    Assignee: Personal Genome Diagnostics Inc.
    Inventor: John F. Thompson
  • Patent number: 11655502
    Abstract: The present disclosure relates to new nucleotide and oligonucleotide compounds and their use in nucleic acid sequencing applications.
    Type: Grant
    Filed: April 12, 2021
    Date of Patent: May 23, 2023
    Assignee: Illumina Cambridge Limited
    Inventors: Elena Cressina, Antoine Francais, Xiaohai Liu
  • Patent number: 11655501
    Abstract: Recognized herein is the need for methods and processes for increasing the efficiency and accuracy of paired end sequencing.
    Type: Grant
    Filed: April 7, 2020
    Date of Patent: May 23, 2023
    Inventors: Gilad Almogy, Mark Pratt, Florian Oberstrass
  • Patent number: 11649489
    Abstract: Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3? end, a polymerase, a reagent 1 for excising the blocking group attached to the 3? end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.
    Type: Grant
    Filed: October 12, 2018
    Date of Patent: May 16, 2023
    Assignee: BGI SHENZHEN
    Inventors: Erkai Liu, Wenwei Zhang, Ao Chen, Chongjun Xu
  • Patent number: 11649484
    Abstract: This invention relates to sequence specific electrochemically-labeled oligonucleotide probes for the detection of nucleic acids and methods associated therewith.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: May 16, 2023
    Inventors: Frank Ray Bowler, Grzegorz Artur Orlowski, Hazel Lucy Greetham, Cheng Zhou, Niall A. Armes, Olaf Piepenburg
  • Patent number: 11643433
    Abstract: The invention relates to the development and optimization of PCR and RT-PCR systems used to detect nucleic acids, including the diagnosis of genetic, viral, and other diseases. The essence of the proposed method is that neutral derivatives of oligonucleotides, namely phosphoryl guanidines containing one or more phosphate groups in which guanidine or substituted guanidine residue is introduced on the phosphorus atom, are used as primers for the template-based amplification, including polymerase chain reaction (PCR) and PCR combined with reverse transcription (RT-PCR). The invention allows to obtain more reliable, specific and selective results in the process of PCR, in particular, to increase the sensitivity of PCR by reducing the yield of by-products of DNA amplification and/or to control the yield of the PCR product, including intentionally suppressing, by using different combinations of the location and number of modified phosphate groups in the oligonucleotide primers.
    Type: Grant
    Filed: September 17, 2018
    Date of Patent: May 9, 2023
    Assignees: “BIOLABMIX” LLC, “NOOGEN” LLC
    Inventors: Maxim Sergeevich Kupryushkin, Inna Alekseevna Pyshnaya, Elena Vladimirovna Dmitrienko, Dmitry Aleksandrovich Stetsenko, Maksim Leonidovich Filipenko, Igor Petrovich Oscorbin, Grigory Aleksandrovich Stepanov, Vladimir Aleksandrovich Richter, Mikhail Konstantinovich Ivanov, Dmitrii Vladimirovich Pyshnyi
  • Patent number: 11634451
    Abstract: Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: April 25, 2023
    Inventors: Floyd E. Romesberg, Denis A. Malyshev, Lingjun Li, Thomas Lavergne, Zhengtao Li
  • Patent number: 11634757
    Abstract: The present invention relates to an emulsion, preferably a water-in-oil emulsion. comprising: a continuous phase comprising a conductivity improving compound, a dispersed phase suspended in the continuous phase, and a surfactant. The present invention also relates to a population of droplets comprising an aqueous phase, dispersed in a continuous oily phase comprising an oil and a conductivity-improving compound. The present invention also relates to a microfluidic chip comprising a hydrophobic composition comprising an oil, a surfactant and a conductivity improving compound in an injection chamber configured so that injecting a hydroplilic composition through said injection means will generate an emulsion in the injection chamber; and to a kit comprising a microfluidic chip and a container comprising an aqueous composition. The present invention also relates to a process for manufacturing an emulsion according to the invention.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: April 25, 2023
    Inventors: Nicolas Fernandez, Étienne Fradet, Rémi Dangla
  • Patent number: 11624089
    Abstract: The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.
    Type: Grant
    Filed: August 20, 2019
    Date of Patent: April 11, 2023
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Stephen Yue, Gene Shen, Wei-Chuan (David) Sun
  • Patent number: 11623940
    Abstract: The invention relates to the use of polyphosphate containing species in a method of nucleic acid synthesis, to methods of nucleic acid synthesis, and to the use of kits comprising said polyphosphate containing species. The invention also relates to the use of polyphosphate containing species for the capping of 3?-terminal hydroxyl moieties using terminal transferases.
    Type: Grant
    Filed: September 14, 2018
    Date of Patent: April 11, 2023
    Assignee: Nuclera Nucleics Ltd.
    Inventors: Michael Chen, Jiahao Huang, Gordon McInroy
  • Patent number: 11618826
    Abstract: The carborhodamine dyes disclosed herein are novel reagents suitable for automated incorporation of carborhodamine dyes into oligonucleotides that can be used in detection methods for nucleic acid targets. This disclosure provides an efficient and simple process for the preparation of carborhodamine compounds and introduces previously unknown reagents for the automated synthesis of oligonucleotide-carborhodamine conjugates.
    Type: Grant
    Filed: September 23, 2021
    Date of Patent: April 4, 2023
    Inventor: Eugeny A. Lukhtanov
  • Patent number: 11613774
    Abstract: The present disclosure relates to a quencher having a quenching effect on a fluorescent material exhibiting luminescence characteristics at an excited energy level, and various uses thereof.
    Type: Grant
    Filed: December 2, 2019
    Date of Patent: March 28, 2023
    Assignee: SFC Co., Ltd.
    Inventors: Goutam Masanta, Bong-Ki Shin, Ju-man Song, Tea-hee Lee, Jong-Tae Je
  • Patent number: 11613772
    Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.
    Type: Grant
    Filed: January 23, 2020
    Date of Patent: March 28, 2023
    Assignee: Quantum-Si Incorporated
    Inventors: Jonathan M. Rothberg, Jeremy Lackey, Brian Reed, Xinghua Shi, Haidong Huang, David Dodd
  • Patent number: 11603388
    Abstract: The present disclosure is directed to conjugates of a specific binding entity and an oligomer, i.e. [Specific Binding Entity]-[Oligomer]n, wherein n is an integer ranging from 1 to 12, and where the Oligomer includes, in some embodiments, a PNA sequence having at least one substituent at a gamma carbon position. In some embodiments, the substituent at the gamma carbon position, e.g. an amino acid, a peptide, a miniPEG, or a polymer, includes at least one reporter moiety.
    Type: Grant
    Filed: June 1, 2020
    Date of Patent: March 14, 2023
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Rui Hong, Aoune Barhoumi
  • Patent number: 11603369
    Abstract: Described herein are divalent nucleobases that each binds two nucleic acid strands, matched or mismatched when incorporated into a nucleic acid or nucleic acid analog backbone, such as in a ?-peptide nucleic acid (?PNA). Also provided are genetic recognition reagents comprising one or more of the divalent nucleobases and a nucleic acid or nucleic acid analog backbone, such as a ?PNA backbone. Uses for the divalent nucleobases and monomers and genetic recognition reagents containing the divalent nucleobases also are provided.
    Type: Grant
    Filed: September 26, 2017
    Date of Patent: March 14, 2023
    Assignee: Carnegie Mellon University
    Inventors: Danith H. Ly, Shivaji A. Thadke, Ali Nakhi, J. Dinithi Rashmini Perera