Patents Examined by John E. Tarcza
  • Patent number: 4468456
    Abstract: A medium for the selective growth and identification of Streptococcus mutans bacteria is disclosed, that includes a tryptone-glucose extract agar, monobasic and dibasic potassium phosphate, yeast extract, agar, a color indicator for the bacteria and a solution of sucrose ranging in concentration from 1% to 15%. Preferably, the concentration of the sucrose may range from about 3% to about 11%. A bacteria culture plate may be prepared comprising the medium, and may include a first basal layer comprised entirely of the medium, and a second overlayer agar coating, including a mixture of the medium with a calcium phosphate suspension. Both the basal layer and the overlayer agar coating are preferably adjusted to a mildly basic pH.The present medium, and bacteria culture plates prepared therewith, offer desired bacterial specificity with no growth inhibition, that is usually the case with specific media of this kind.
    Type: Grant
    Filed: March 29, 1982
    Date of Patent: August 28, 1984
    Assignee: Warner-Lambert Company
    Inventor: John L. Deyloff
  • Patent number: 4464465
    Abstract: Novel method, cells and compositions are provided involving transforming B-lymphocytes to provide immortalization for continuous production of monoclonal antibodies to a predetermined ligand. T-cell free B-lymphocytes are combined with an Epstein-Barr virus transformed cell sensitive to a cytotoxic agent which does not significantly affect the B-lymphocytes under conditions where the sensitive EBV transformed cell acting as the transfer agent is killed and efficiently transforms the B-lymphocyte recipient cells with EBV. The EBV transformed B-lymphocyte cells are amplified and cloned, the desired clones isolated in accordance with conventional techniques and then used for production of monoclonal antibodies.
    Type: Grant
    Filed: April 5, 1982
    Date of Patent: August 7, 1984
    Assignee: Genetic Systems Corporation
    Inventor: Mark E. Lostrom
  • Patent number: 4460765
    Abstract: A new compound, named "Griseolic acid", and its salts and can be prepared by the cultivation of Streptomyces griseoaurantiacus SANK 63479 (FERM-P 5223). Griseolic acid and its salts inhibit the activity of the enzyme cyclic adenosine monophosphate phosphodiesterase and, as a result of this, have variety of physiological activities and uses.
    Type: Grant
    Filed: May 12, 1982
    Date of Patent: July 17, 1984
    Assignee: Sankyo Company Limited
    Inventors: Atsushi Naito, Fumio Nakagawa, Takao Okazaki, Akira Terahara, Seigo Iwado, Mitsuo Yamazaki
  • Patent number: 4454227
    Abstract: Cultivation and reproduction of insect cells in a nutrient medium in which fetal calf serum is partially or completely replaced by egg yolk.
    Type: Grant
    Filed: December 18, 1981
    Date of Patent: June 12, 1984
    Assignee: Hoechst Aktiengesellschaft
    Inventor: Anton Roder
  • Patent number: 4452891
    Abstract: Mycophenolic acid is produced by aerobically culturing in a culture medium a mutant of the genus Penicillium capable of producing mycophenolic acid, said mutant being resistant to clofibrat, and recovering the mycophenolic acid which accumulates in the culture medium.
    Type: Grant
    Filed: September 8, 1981
    Date of Patent: June 5, 1984
    Assignee: Ajinomoto Company Incorporated
    Inventors: Takao Kida, Takehiko Ishikawa, Hiroshiro Shibai
  • Patent number: 4448886
    Abstract: A process for producing high dispersion metal crystallites dispersed substantially homogeneously in a carbonaceous material matrix is disclosed. The novel metal crystallite dispersions disclosed herein have the added advantage of being locked into the matrix thereby preventing migration of the metal crystallites.
    Type: Grant
    Filed: November 30, 1981
    Date of Patent: May 15, 1984
    Assignee: Diamond Shamrock Corporation
    Inventors: Lawrence J. Gestaut, P. David Simcox
  • Patent number: 4439525
    Abstract: Mutant yeasts of the strain Pichia pastoris have been developed which contain relatively high levels of methionine. These high methionine content Pichia pastoris mutants grow on an oxygenated hydrocarbon such as methanol, to produce improved amino acid balance single-cell protein product eliminating or reducing the need to supplement single-cell protein with methionine when used as food supplements.
    Type: Grant
    Filed: September 9, 1981
    Date of Patent: March 27, 1984
    Assignee: Phillips Petroleum Company
    Inventors: Lucas K. Shay, Eugene H. Wegner
  • Patent number: 4436816
    Abstract: Novel compositions for use as cell growth-promoting materials are made by the following novel process involving the steps of:(a) slowly contacting serum or plasma with sufficient chilled perchloric acid to reach a 0.1 to 0.25 final molar concentration of said perchloric acid in said serum or plasma,(b) at a temperature of -1.degree. C. to 15.degree. C.
    Type: Grant
    Filed: January 22, 1982
    Date of Patent: March 13, 1984
    Assignee: Pitman-Moore, Inc.
    Inventor: Stephen K. Dinka
  • Patent number: 4434227
    Abstract: A method for determining an immunoglobulin of the IgX class in a sample where X is either M, A, D or E. Anti-IgG is added to IgG to prevent binding of rheumatoid factor before the sample containing IgX is added to insolubilized IgG.
    Type: Grant
    Filed: February 8, 1982
    Date of Patent: February 28, 1984
    Assignee: Abbott Laboratories
    Inventor: John T. Unger
  • Patent number: 4431738
    Abstract: In a plant tissue and cell cultivation, a method for facilitating both cell multiplication and differentiation is attained by conducting the cultivation in a culture medium containing an extract of micro algae such as Chlorella, Scenedesmus and Spirulina.
    Type: Grant
    Filed: September 21, 1981
    Date of Patent: February 14, 1984
    Assignee: Chlorella Industry Co., Ltd.
    Inventors: Tadahiko Maeda, Kuniaki Tanaka
  • Patent number: 4427656
    Abstract: The present invention refers to a chlorine containing antibiotic substance, named antibiotic A/16686 factor A.sub.2, in an essentially pure form. The invention also relates to the process for the production of antibiotic A/16686 factors A.sub.2 by cultivation of an Actinoplanes strain and to the co-produced antibiotic A/16686 factos A.sub.1 and A.sub.3. Antibiotic A/16686 factors A.sub.1, A.sub.2 and A.sub.3 as well as the corresponding non-toxic physiologically acceptable acid addition salts are antimicrobial agents particularly active against gram-positive bacteria.
    Type: Grant
    Filed: July 31, 1981
    Date of Patent: January 24, 1984
    Assignee: Gruppo Lepetit S.p.A.
    Inventors: Bruno Cavalleri, Enrico Selva
  • Patent number: 4423145
    Abstract: Methods are disclosed for isolating and culturing human mammary epithelial cells of both normal and malignant origin. Tissue samples are digested with a mixture including the enzymes collagenase and hyaluronidase to produce clumps of cells substantially free from stroma and other undesired cellular material. Growing the clumps of cells in mass culture in an enriched medium containing particular growth factors allows for active cell proliferation and subculture. Clonal culture having plating efficiencies of up to 40% or greater may be obtained using individual cells derived from the mass culture by plating the cells on appropriate substrates in the enriched media. The clonal growth of cells so obtained is suitable for a quantitative assessment of the cytotoxicity of particular treatment. An exemplary assay for assessing the cytotoxicity of the drug adriamycin is presented.
    Type: Grant
    Filed: May 7, 1981
    Date of Patent: December 27, 1983
    Inventors: Martha R. Stampfer, Helene S. Smith, Adeline J. Hackett
  • Patent number: 4390630
    Abstract: Novel fibrinolytic factors obtained from the salivary glands of Haementeria ghilianii. Proteins having molecular weights under about 100,000 are isolated from the salivary glands of H. ghilianii. The proteins show cathodic mobility in electrophoresis and uninhibited peptidase activity with fibrinogen in plasma.
    Type: Grant
    Filed: October 28, 1980
    Date of Patent: June 28, 1983
    Assignee: The Regents of the University of California
    Inventors: Roy T. Sawyer, Gunther S. Stent, Andrei Z. Budzynski, Stephanie A. Olexa
  • Patent number: 4376163
    Abstract: This invention relates to a process for producing ethanol by continuous fermentation of polysaccharide-containing raw materials. The fermentation is carried out in one or several fermentors, a stream of fermentation liquor being separated into a yeast concentrate stream and a yeast-free stream, and possibly one sludge stream, of which the yeast concentrate stream is recirculated to the fermentor, at least part of the yeast-free stream being fed to a simple evaporator unit, corresponding to one or a few distilling stages, wherein it is separated, partly into a first vapor stream, enriched in ethanol, which is fed to a plant for production of the desired ethanol grade, and partly into a first liquid bottom stream, which is at least in part recirculated to the fermentor.
    Type: Grant
    Filed: September 23, 1980
    Date of Patent: March 8, 1983
    Assignee: Alfa-Laval AB
    Inventor: Lars K. J. Ehnstrom
  • Patent number: 4374859
    Abstract: A method and microorganism are set out for producing a fermentation product which contains ethanol but has a lower than usual fusel oil content. The mutant microorganism is of the genus Saccharomyces or Torulaspora.
    Type: Grant
    Filed: July 7, 1980
    Date of Patent: February 22, 1983
    Assignee: The Regents of The University of California
    Inventors: Ralph E. Kunkee, S. Richard Snow, Craig Rous
  • Patent number: 4368261
    Abstract: A method for the determination of triglycerides by ester cleavage utilizing lipase and optionally esterase with the formation of fatty acids and glycerol, phosphorylation of the glycerol with adnosine triphosphate in the presence of glycerol kinase with the formation of glycerol-1-phosphate and adenosine diphosphate and determination of one of the latter two products. The glycerol kinase used is from Bacillus stearothermophilis and acts in combination with at least one activator selected from the group consisting of detergents, phenol derivatives and aniline derivative.
    Type: Grant
    Filed: December 5, 1980
    Date of Patent: January 11, 1983
    Assignee: Boehringer Mannheim GmbH
    Inventors: Sigmar Klose, Albert Roder, Walter Schneider
  • Patent number: 4361651
    Abstract: Disclosed is a dry or wet milling process for making fermentable sugars and high-protein products from starch bearing material, characterized by saccharification of the starch followed by recovery of fiber and other non-protein materials and of destarched protein as separate products, leaving a sugar solution that is essentially free of insoluble materials. The sugars are fermented to ethanol and carbon dioxide by the action of added yeast. After fermentation, the yeast is recovered for use in fermenting additional quantities of sugars supplied either for batch or continuous processing. The alcohol is then removed leaving a dilute solution of unfermentable soluble materials, and this liquid, in whole or in part, is recycled to preceding steps in the process.
    Type: Grant
    Filed: July 18, 1980
    Date of Patent: November 30, 1982
    Inventor: Carroll R. Keim
  • Patent number: 4359533
    Abstract: An improved fermentation process for producing alcohol which includes the combination of vacuum fermentation and vacuum distillation. Preferably, the vacuum distillation is carried out in two phases, one a fermentor proper operated at atmospheric pressure and a flash phase operated at reduced pressure with recycle of fermentation brew having a reduced alcohol content to the fermentor, using vapor recompression heating of the flash-pot recycle stream to heat the flash-pot or the distillation step, and using "water load balancing" (i.e., the molar ratio of water in the fermentor feed is the same as the molar ratio of water in the distillation overhead).
    Type: Grant
    Filed: November 26, 1980
    Date of Patent: November 16, 1982
    Assignee: The United States of America as represented by the Department of Energy
    Inventors: Charles R. Wilke, Brian L. Maiorella, Harvey W. Blanch, Gerald R. Cysewski
  • Patent number: 4358537
    Abstract: This invention relates to a process for in situ biological beneficiation of peat and hydraulic harvesting of organic carbonaceous material from a peat deposit. The process is particularly suitable for production of hydrocarbon fuel from peat wherein peat is contact in situ in a peat deposit with an aqueous solution of hydrolysis promoting agent hydrolyzing organic carbonaceous components of the peat to produce hydrolysis products, passing the hydrolysis products to an active fermentation zone in situ in the peat deposit to produce fermentation products, passing the fermentation products, remaining hydrolysis products and organic carbonaceous materials leached from the peat by the aqueous solution to a biological or chemical process for conversion to hydrocarbon fuel. This process provides peat deposit management while providing beneficiation of the carbonaceous material for improved conversion efficiency by chemical or biological conversion to hydrocarbon liquid or gaseous fuels.
    Type: Grant
    Filed: October 22, 1980
    Date of Patent: November 9, 1982
    Assignee: Institute of Gas Technology
    Inventor: David P. Chynoweth
  • Patent number: 4357424
    Abstract: A process for the continuous production of fermentation alcohol, by effecting fermentation of a continuous or substantially continuous supply of the liquid substrate by a dense suspension of a suitable micro-organism in a reaction column wherein the suspension is maintained in a well mixed state. The mixture passes from the upper region of the reaction column into a degassing zone where less turbulent conditions readily permit degassing of the mixture, causing part of the degassed mixture to flow into a settling zone wherein quiescent conditions permit the biomass to settle out. The settled biomass is returned to the bottom of the reaction column to assist in the continuation of the fermentation process. Gases evolving from the top of the reaction column and from the tops of the degassing and settling zones are removed.
    Type: Grant
    Filed: December 2, 1980
    Date of Patent: November 2, 1982
    Assignee: Sim-Chem Limited
    Inventor: John D. Bu'Lock