Abstract: Methods are provided for producing fusion partners which involve employing an immortalized human myeloma cell line sensitive to HAT and having an additional dominant selectable resistance marker and fusing the doubly marked human myeloma cells with a stable immortalized rodent myeloma cell line, desirably previously subjected to substantial chromosome damage, and isolating cells having a substantially complete chromosomal complement of the rodent cell and at least about one chromosome of the human cell having a gene expressing said resistance, thereby being resistant to a selective agent. The resulting heteromyeloma may be fused with high efficiency with human lymphocytes to produce monoclonal antibodies.The cell lines designated as A6 and 36 were deposited at the A.T.C.C. on Jan. 11, 1983 and given accession numbers CRL8192 and CRL8193, respectively.

Abstract: This invention relates to processes which are used to produce, isolate, and characterize human rotavirus/animal rotavirus reassortants and to produce live attenuated vaccines and vaccine precursors. In the present strategy there is involved the new use of either (1) high titer hyperimmune antisera or (2) monoclonal antisera to select reassortants with the desired human phenotype. A point of novelty is the finding that antiserum or monoclonal antisera alone, so long as it possesses high titer neutralizing activity against only the 34-38Kd glycoprotein or of the animal parent, is sufficient to use for selection of reassortant rotaviruses with human phenotype. Also, the novel products are live attenuated vaccine precursors and vaccines.

Abstract: On the new findings that cyclodextrin shows no cytotoxicity or only slight cytotoxicity on cell growth and that lipophilic substances such as unsaturated fatty acids and lipophilic vitamins when present together with, or included in, cyclodextrin show such effects as cell growth promoting effect and accelerating effect of the productivity of valuable products, a serum-free or serum-reduced culture medium or a substitute composition for serum for a culture medium comprising a cyclodextrin and at least one lipophilic nutrient substance, said cyclodextrin and said lipophilic nutrient substance being preferably in the form of inclusion complex between them, is provided.

Abstract: Human hybridomas producing a preselected human monoclonal antibody are prepared by fusing human lymphocytes with a hybrid fusion partner. The hybrid fusion partner is the result of fusing human lymphocytes with human myeloma cells at least once wherein the resulting hybrid cell is capable of being a functional human fusion partner.

Abstract: Monoclonal antibodies demonstrating a reactivity with human breast cancer are produced. The hybridoma cultures secreting immunoglobins are produced by hydridoma technology. Splenic lymphocytes of mice, immunized with membrane-enriched fractions of metastatic human mammary carcinoma tissue are fused with the NS-1 non-immunoglobulin-secreting murine myeloma cell line. Screening of immunoglobulin reactivities and double cloning of cultures yielded 11 monoclonal antibodies that demonstrated activities with the surface of human mammary tumor cells and not with the surface of apparently normal human tissues. These monoclonal antibodies aid in the diagnosis, prognosis and treatment of human breast cancer.

Abstract: Microbial synthesis of indigo dyestuff in indole-free media is disclosed. Indigo production is preferably accomplished by genetic transformation of selected host cells having the capacity to produce and accumulate indole (either as a result of endogenous genomic capacity or genetic transformation) to incorporate the capacity for synthesis of an aromatic dioxygenase enzyme. Growth of transformed cells under suitable conditions facilitates aromatic dioxygenase enzyme catalyzed oxidative transformation of cellular indole, with consequent formation of indigo from the oxidized reaction products. In a highly preferred embodiment, E. coli cells having endogenous indole production capacity are transformed with a DNA expression vector comprising the structural gene for naphthalene dioxygenase, resulting in the microbial synthesis of isolatable quantities of indigo.

Abstract: A new strain of Pseudomonas fluorescens is disclosed for use in the removal of chromate from waste water.

Abstract: A method of culture of human cells is disclosed which comprises effecting the cultivation in a culture medium containing an extract of micro algae, such as Chlorella, Scenedesmus or Spirulina, said method permitting the normal successive cultivation of human cells to be maintained efficiently without any morphological and genetic mutations over a greater number of successive of generations than has hitherto been possible even by the incorporation of animal serum in the culture medium, even when the addition amount of such animal serum is reduced substantially or animal serum is completely excluded.

Abstract: A non-transformed cell line is produced by treating BALB/c derived 10E2 cells with 5-bromodeoxyuridine and 5-iodo-2'-deoxyuridine to produce thymidine kinaseless cells which upon multiple cloning show a flat epitheloid appearance indicative of their non-transforming potential. These cells are used to determine the tumorigenic transforming potential of any gene by introducing the gene into the cells of the non-transformed cell line along with the Herpes simplex virus thymidine kinase gene which serves as a vehicle for cotransfection. The transformation of the cells is indicative of tumorigenic potential.

Abstract: An enzyme immunoassay for detecting an antigen in a biologic fluid or tissue which comprises contacting the fluid or tissue with an antibody specific for the antigen under binding conditions, at least one of the fluid or tissue and antibody having a solid component, contacting the resulting solid with a conjugate bindable with the antibody under binding conditions and determining the enzyme activity of the resulting solid phase is described. The conjugate is of peroxidase and an allergen, non-immunoglobulin protein or primary amino group containing drug having an average of 2-3 molecules of peroxidase per molecule of substance with an average molecular weight of about 30,000 daltons, prepared by reacting peroxidase previously treated with phenyl isothiocyanate and oxidized to form aldehyde groups with the substance to form a Schiff's base which is titrated with a reducing agent to form a stable conjugate.

Abstract: Compositions of selected strains of Pseudomonas bacteria having the ability to utilize halogenated aromatic compounds as a sole carbon source are described. The bacteria are isolated from environments where they have been in long association with halogenated aromatic compounds, usually analagous compounds. First L-tryptophan and then a halogenated aromatic hydrocarbon are used as sole carbon sources for isolating and testing the selected strains. The isolated Pseudomonas strains are Pseudomonas putida; Pseudomonas sp. NRRL-B-12,538 or NRRL-B-12,539 or transfer derivatives thereof and are useful for degrading halogenated aromatic pollutants, particularly mono- and di-chloroaromatics.

Abstract: Novel human lymphoblastoid cells and hybridomas derived therefrom are provided. The cells are a HGPRT negative human B-cell line. The cells are readily fusible with lymphoid cells to produce hybridomas which secrete human monoclonal antibodies.

Abstract: An immunoassay is disclosed where the label is an enzyme which converts a precursor into a cycling factor which in turn is interconverted in a cycling detection system. The detection system is amplified by the enzyme label constantly increasing the amount of cycling factors in the system. The system of NADP to NAD with NAD cycling to NADH and back is preferred.

Abstract: Efficiency of process for producing H.sub.2 by subjecting algae in an aqueous phase to light irradiation is increased by culturing algae which has been bleached during a first period of irradiation in a culture medium in an aerobic atmosphere until it has regained color and then subjecting this algae to a second period of irradiation wherein hydrogen is produced at an enhanced rate.

Abstract: A human non-secretory plasmacytoid continuous cell line, established for five years in more than 150 passages, is karyotypically normal, easily grown and has the characteristic features of a plasmablast excepting for its secretory defect, and can be used for the preparation of human-human hybridomas with human B-lymphocytes and separation of the resulting hybridomas from the plasmacytoma cell line by growth in CO.sub.2 -containing media, or by fluorescence activated cell sorting, or both.

Abstract: Transfusable type O erythrocytes free of P1 antigenicity which are produced by an alpha-galactosidase conversion of type B antigen to type H antigen. The resulting erythrocytes retain a high level of ATP and 2,3 DPG.

Abstract: Methods featuring, in one aspect, a method of preparing an antigen or mixture of antigens substantially free of antigens specific to at least two monoclonal antibodies, said method involving contacting an antigen mixture with one or more previously-isolated monoclonal antibodies to form complexes between those antibodies and antigens present in the mixture specific to the antibodies, removing the complexes from the antigen mixture to yield a partially purified antigen mixture, immunizing an animal with the partially purified antigen mixture, fusing spleen cells from the immunized animal to myeloma cells to form hybridomas capable of producing additional monoclonal antibodies, culturing said hybridomas to produce said additional monoclonal antibodies, contacting a sample of the partially purified antigen mixture with said additional monoclonal antibodies to form complexes between said additional monoclonal antibodies and antigens present in the antigen mixture specific to the additional monoclonal antibodies,

Abstract: There is disclosed a macrolide isolated from the fermentation broth of a microorganism identified as MA-5285 which morphological analysis reveals to be a strain of Streptomyces hygroscopicus. The compound's structure is presented based upon analytical studies. The compound has insecticidal and antiparasitic activity.

Abstract: A biologically active substance is incorporated in a unitary body with a magnetically responsive material for carrying out diffusion testing. These may be, microbiological, immunological, serological and other biochemical examinations. The body is applied against a substrate or medium by application of an external magnetic field and a reaction region is produced at the site of the body and is measured by means of a reader. In order to insure deposit of the body on the substrate a predetermined location and corresponding reading of the reaction region at such location, the support for the substrate and the dispenser and rear are provided with suitable releasable coupling and orienting devices such that the dispenser and reader can be respectively engaged and oriented on the support in predetermined secured positions.

Abstract: A method for enhancing the production of interferon from normal human diploid fibroblast cells is disclosed. In this method, a distinct interferon-production phase is established in which the temperature is initially elevated for a brief period followed by a reduction in temperature for the balance of the interferon-production phase.