Abstract: A diffractive optical element device for use in spectroscopy, where broadband light is emitted from a light source (31) towards the optical element (24) and from there is transmitted to at least one detector (29; 29?). The optical element has a plurality of diffractive dispersively focusing patterns, preferably partly integrated into each other, whose respective centers are two-dimensionally offset relative to each other in order to produce a plurality of spectra (25–28), where at least two are separate, but offset relative to each other and/or partly overlapping. In an alternative embodiment, the optical element consists of either one diffractive optical element (60) that is related to a wavelength and produces a spectrum, or at least two diffractive optical elements (60, 61) which are related to respective wavelengths and which produce at least two mutually partly overlapping spectra to give a composite spectrum.

Abstract: A light processor (such as a spectrometer) providing wavelength equalization for a sample pathway and a reference pathway by actuation of a light amplitude modulator. A chemometric processor including a light amplitude modulator capable of performing chemical analysis by applying weights to wavelengths of light, thereby reducing the need for electronic post processing.

Abstract: Real time biofilm monitoring systems are provided. Said systems comprise single or multiple fiber-optic probes detecting wavelength-specific fluorescence from biomarkers of fouling organisms; a compact optoelectronic interface and data acquisition system interfaced with said probes, wherein said probe or probes are bifurcated and contain at least one excitation and at least one emission filter permitting the simultaneous resolution of multiple biomarkers.

Abstract: Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Abstract: A cell tray has a multi-dimensional array of cells in precise, equally spaced wells (cubicles or silos) containing medium of interest. The ordered cell array enables automated processing as well as simultaneous monitoring and analyzing of a large matrix of cells, biological fluids, chemicals and/or solid samples. The invention is an integrated device and is fabricated into substrates similar to microscope slides. The ordered array of cells in precise locations helps in parallel analysis and processing of cells simultaneously. Each cell cubicle or silo in the array is located equidistant from its nearest neighbors in an orthogonal direction. The location of each well can be precisely measured and recorded in an automated processing system. Included in the bottom of each cell well is an optional micro-lens. An array of probes provides parallel cell processing and monitoring capabilities, including microinjection and microscope analysis.

Abstract: Methods and apparatus for generating a frequency comb and for its use in analyzing materials and in telecommunications. The frequency comb is generated by passing pulsed light from a laser through an optical fiber having a constriction. The frequency comb comprises a plurality of monochromatic components separated in frequency by a substantially constant frequency increment. The monochromatic components are used to probe materials for analysis. In preferred embodiments, the materials are DNA, RNA, PNA and other biologically important molecules and polymers. Optical responses are observed and used to analyze or identify samples. In telecommunication applications, the individual monochromatic components serve as carriers for individual communication channels that can carry information of any of a variety of types, such as voice, data and images.

Abstract: A high scan rate spectroscopic system converts a narrow-band laser pulse into a multispectral pulse, using, for example, a nonlinear fiber. The multispectral pulse is then converted to a swept frequency pulse through a second fiber impressing a frequency-dependent delay in the light beam which is then applied to the object to be tested.

Abstract: A device for measuring the intensity of a luminescence radiation emitted by at least one chemical species probed, along a trajectory by an excitation radiation. The device includes n measurement channels, where n is an integer greater than or equal to 2, each measurement channel being used to measure a fraction of the intensity of the luminescence radiation emitted along the trajectory. The device is also applicable to analytical chemistry to make speciation measurements.

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An instrument system for detecting a biochemical interaction on a biosensor. The system includes an array of detection locations comprises a light source for generating collimated white light. A beam splitter directs the collimated white light towards a surface of a sensor corresponding to the detector locations. A detection system includes an imaging spectrometer receiving the reflected light and generating an image of the reflected light.

Abstract: Multi-ordered spectral data is obtained from various known substances and is stored in a spectral library. The identification of an unknown material is accomplished by correlating the sample's multi-ordered spectrum against all or a portion of the spectrum in the library, and finding the closest match.

Abstract: An optical system and method of exciting and measuring fluorescence on or in samples treated using fluorescent pigments using such an optical system having at least one first laser (1); a mirror (4); a deflection element (7); an optic (8); and a unit (10), which includes mirror (4) and optic (8) mounted fixed in relation to one another. The unit (10) is positioned so it is linearly movable back and forth along the optical axis (5) and is mechanically connected to an oscillating linear drive (11). The optic (8) additionally acts as a collimator and the mirror (4) additionally acts to deflect the collimated light (12) parallel to the optical axis (5).

Abstract: A small-spot imaging, spectrometry instrument for measuring properties of a sample has a polarization-scrambling element, such as a birefringent plate depolarizer, incorporated between the polarization-introducing components of the system, such as the beamsplitter, and the microscope objective of the system. The plate depolarizer varies polarization with wavelength, and may be a Lyot depolarizer with two plates, or a depolarizer with more than two plates (such as a three-plate depolarizer). Sinusoidal perturbation in the resulting measured spectrum can be removed by data processing techniques or, if the depolarizer is thick or highly birefringent, the perturbation may be narrower than the wavelength resolution of the instrument.

Abstract: A method and apparatus for obtaining in-situ data of a substrate in a semiconductor substrate processing chamber is provided. The apparatus includes an optics assembly for acquiring data regarding a substrate and an actuator assembly adapted to laterally move the optics assembly in two dimensions relative to the substrate.

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.

Abstract: In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, and the transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.

Abstract: Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.

Abstract: A spectroscopy method in which a sample is scanned without moving the sample. Light from the sample 16 is collected by a lens 14 and analyzed at a spectrum analyzer 28 before being focused onto a photodetector 32. Light from the focal point of the lens 14 is brought to a tight focus on the photodetector 32 whilst light from in front of or behind the focal point comes to a more diffuse focus. Light from the pixels on the photodetector 32 corresponding to the focal point of the lens 14 is processed, whilst light from pixels outside this region is ignored, thus forming a ‘virtual slit’. The sample 16 is scanned in a vertical direction by moving the ‘virtual slit’ up and down, by changing the designated rows of pixels from which data is analyzed. The sample is scanned in a horizontal direction by moving a vertical slit 24 in the light path in a horizontal direction.

Abstract: Methods and apparatuses for testing image sensors are disclosed. Desirable apparatuses of the present invention include image sensor testing devices comprising a digital light projection system capable of projecting static or dynamic images onto an image sensing device under test and an image sensor signal detection means for analyzing the output of said image sensing device under test. The digital light projection system comprises a light source, collimating optics, a digital micromirror device, and focusing optics. Other desirable methods and apparatuses of the present invention include image sensor testing devices employing a digital light projection system capable of simultaneously testing a plurality of image sensors. According to the present invention, the light source is calibrated and converted to a desired test image by the digital micromirror device. The test image is then focused onto an image sensor, the output of which is read by a detector and correlated with the input digital test image.

Abstract: Methods for the continuous determination of the properties of a flow of wood fibers for use in the production of fiberboard are disclosed including determining reference value characteristics for calibration samples of the fibers, determining predetermined relationships between those reference value characteristics and measured spectral values for the fibers utilizing multivariant statistical regression methods, measuring reflectance spectral values for the fibers illuminated by light, and determining the fiber characteristics relating to the properties from the measured spectral values utilizing the predetermined relationship.