Abstract: A cross-flow filtration device includes: a filter module which includes an inner chamber and an outer chamber separated by a semipermeable membrane; a process liquid tank which is configured to accommodate a process liquid; a pump which is configured to cause the process liquid to circulate to the inner chamber in the filter module and the process liquid tank; a replenisher tank which is configured to accommodate a replenisher liquid to be replenished into the process liquid tank, at least one or more sensors which are configured to measure a pressure of the circulating process liquid; and a replenisher measurement unit which is configured to measure an amount of the replenisher liquid supplied from the replenisher tank to the process liquid tank. The process liquid tank is configured to be continuously replenished with the replenisher liquid.

Abstract: A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube (3,19,21) having a closed first end (5) comprising an inlet/outlet (7), a media inlet (15) adjacent a second end of the column tube and an adaptor (9) positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.

Abstract: The present invention relates to an adsorption medium including polymeric space elements which have been bonded to the surface of the chromatography matrix, and polymer chains containing chromatographically active centers, wherein the polymer chains have been bonded to the polymeric spacer elements, as well as to a method for the production thereof, and to the use of the adsorption medium for the purification of biomolecules.

Abstract: The purpose of the present invention is to adsorb calciprotein particles. An adsorbent for calciprotein particles of the present invention is characterized in that the surface of a water-insoluble carrier is covalently bonded, through a hydrocarbon group, to at least one selected from the group consisting of amino group, carboxyl group, phosphate group, phosphono group, phosphino group, and thiol group.

Abstract: A method of performing experiments on samples may include receiving criteria defining a starting state for performing an experiment in a system including a scientific instrument; determining whether the starting state is established in the system; and, responsive to the starting state being established, allowing the experiment, that analyzes a sample using the scientific instrument, to proceed. Determining whether the starting state is established may include automated monitoring of at least one parameter for at least one data channel, and determining whether all such parameters simultaneously meet associated conditions of the criteria for specified time periods. The method may include automatically monitoring the system and re-establishing the starting state prior to performing each of one or more subsequent experiments. The method may include automatically monitoring and establishing a second starting state prior to performing each of one or more additional experiments.

Abstract: Determining optimum operating binding capacity for a MCC process using one column, the process including N number of columns, comprises loading target product on a first column at a first residence time and/or flow rate; loading the product on the column at a second residence time and/or flow rate, the first residence time and/or flow rate being different than the second residence time and/or flow rate; generating a first breakthrough curve for the first residence time and/or flow rate and a second breakthrough curve for the second residence time and/or flow rate, wherein the first curve represents product breakthrough for the first column and the second curve represents product breakthrough for an Nth column; and using the curves to determine product loading capacity of the first column before product breakthrough at the Nth column; the product loading capacity of the first column equaling optimum operating binding capacity for the process.

Abstract: An extraction cell (10) for a centrifugal partition chromatograph (20), which extraction cell (10) contains an extraction chamber (12) delimited by a cell wall (12c) and accommodates the liquid stationary phase (30á), and it has a liquid inlet opening (13b) and a liquid outlet opening (13k) serving to let in and out the liquid mobile phase (30m) to be made to flow through the extraction cell (10). The extraction cell (10) contains an extraction chamber (12) established as a tubular body, and a liquid inlet plug (16b) that includes a liquid inlet opening (13b) and a liquid outlet plug (16k) that includes a liquid outlet opening (13k), that can be attached to the extraction chamber (12) and an insert (14) through which liquids may pass is positioned in the extraction chamber (12) between the liquid inlet opening (13b) and the liquid outlet opening (13k).

Abstract: A system and method for purifying coenzyme Q10 are provided. The method includes: passing a CoQ10-containing crude product through a first chromatographic column to obtain a first CoQ10-containing intermediate product. The method further includes preparing, based on the first CoQ10-containing intermediate product, a second CoQ10-containing intermediate product. The method further includes passing the second CoQ10-containing intermediate product through a second chromatographic column to obtain a third CoQ10-containing intermediate product. The method further includes obtaining purified CoQ10 product by purifying the third CoQ10-containing intermediate product.

Abstract: The present invention relates to a method of purifying peptides and/or polypeptides, said method comprising or consisting of: (a) loading a sample comprising peptides and/or polypeptides under acidic or neutral aqueous conditions on mixed-phase solid phase extraction (SPE) material, wherein said material consists of or comprises reversed phase/ion exchange material; (b) washing said mixed-phase SPE material with (ba) an acidic or neutral composition comprising at least 50% (v/v) organic solvent; and/or (bb) an acidic or neutral aqueous solution; and (c) eluting said peptides and/or polypeptides from said mixed-phase SPE material with an alkaline composition comprising at least 50% (v/v) organic solvent.

Abstract: Chromatography and synthesis columns, assemblies, components, and methods of assembly and disassembly are disclosed including a support assembly having lifting mechanisms in each of the legs to raise and lower a frame secured to the column, a swing arm and/or carriage guiding movement of a bottom plate of the column, a bolt-free coupling between the main tube and bottom plate of the column, and upper and lower slurry ports in the main tube of the column.

Abstract: A method of separating analytes in a liquid sample includes flowing the liquid sample through a chromatography column configured for use in liquid chromatography. The chromatography column contains a composition that includes a solid support having an exterior surface, a ligand, and a linker. The ligand includes a polyhedral oligomeric silsesquioxane moiety. The linker is covalently bound to both the polyhedral oligomeric silsesquioxane moiety and the exterior surface of the solid support.

Abstract: A method for producing a chemical reactor, wherein the chemical reactor comprises one or more effective channels which comprise pillar structures, an input connected to one of the effective channels to allow fluid/gas into the effective channels and an output connected to one of the effective channels to remove at least one component of the liquid/gas. The method comprises obtaining an initial design of the reactor, further introducing into the initial design at least a structured area positioned adjacent to an effective channel of the one or more effective channels located at the edge of the initial design, the structured area not being fluidly connected to one of the effective channels, to obtain a further design and the production of the reactor according to the further design.

Abstract: The invention relates to the filtering of liquid samples, preferably in a laboratory environment, for example for collecting micro organisms from the liquid sample for subsequent testing. The invention provides a method for filtering a liquid sample which comprises providing a blank of a deformable sheet material and a filtration medium, deforming the blank to form a funnel extending above and about the filtration medium, introducing the liquid sample to be filtered into the so formed funnel, and filtering the liquid sample from the funnel through the filtration medium, for example to a downstream receptacle. The method is accordingly based on the concept that the funnel is not or at least not completely preformed but provided in a form of a blank of a deformable material sheet which is deformed so as to form the funnel at the point and time of use during filtration.

Abstract: A membrane sorbent is described, which comprises 1-6 wt % silicon carbide nanoparticles dispersed in a polymer matrix. The polymer matrix may comprise polysulfone and polyvinylpyrrolidone. The membrane sorbent is used for separating oil from a contaminated water mixture. The silicon carbide nanoparticles of the membrane sorbent may be made from rice husk ash.

Abstract: Devices and associated processes suitable for small and large scale purification of molecules in a fluid, and provides embodiments that overcome the difficulties of transitioning purification from research stages to production scale-up. The invention relates to the formation and characteristics of substantially uniform, continuous, and homogeneous porous separation matrices. The matrices are in the form of a polymeric layer with substantially uniform thickness and porosity. The polymeric layer is formed for the purpose of providing convective flow through the separation matrix.

Abstract: The present invention pertains to inter alia methods for purifying extracellular vesicles including exposing a sample comprising at least one extracellular vesicle to ultrafiltration; and exposing the sample following the ultrafiltration in step (i) to size exclusion liquid chromatography.

Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

Abstract: Chromatography devices contain chromatography media and methods of making and methods of using chromatography devices. Chromatography devices enable a more efficient, productive and/or environmentally friendly chromatographic operation due to one or more of the following advantages over conventional chromatographic operations: elimination of a device packing step by the user; elimination of clean-in-place (CIP) steps; elimination of clean-in-place (CIP) steps utilizing sodium hydroxide solution; elimination of any validation steps by the user; and use of a chromatography device comprising biodegradable material. The chromatography media includes porous inorganic particles having a functionalized surface and having a median pore size of at least about 300 Angstroms (A), or at least about 300 A up to about 3000 A. The inorganic particles may have a BET surface area of at least about 20 m2/g, or at least about 25 m2/g, or about 30 m2/g, up to about 2000 m2/g.

Abstract: A composite material of polyurethane foam having a layer of reduced graphene oxide and polystyrene is described. This composite material may be made by contacting a polyurethane foam with a suspension of reduced graphene oxide, drying, and then irradiating in the presence of styrene vapor. The composite material has a hydrophobic surface that may be exploited for separating a nonpolar phase, such as oil, from an aqueous solution.

Abstract: The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.