Abstract: The invention relates to a device for performing binding assays. In particular, the invention relates to a centrifugal device for performing such assays. The invention also relates to a method of performing binding assays involving antigen-antibody binding, nucleic acid hybridization, or receptor-ligand interaction.
Type:
Grant
Filed:
November 20, 2003
Date of Patent:
December 20, 2011
Assignee:
Corbett Life Science Pty Ltd
Inventors:
John Michael Corbett, John Michael Corbett, Jr.
Abstract: A method of detecting ?-defensin in a bodily sample from a subject includes reducing the electrostatic interaction between ?-defensin and negatively charged moieties in the bodily sample prior to detecting the ?-defensin with an antibody or epitope binding fragment thereof.
Abstract: A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.
Type:
Grant
Filed:
January 18, 2005
Date of Patent:
December 13, 2011
Assignee:
Biocept, Inc.
Inventors:
Zhongliang Tang, Ram S. Bhatt, Pavel Tsinberg
Abstract: The invention relates to methods for diagnosing rheumatoid arthritis by assaying for Fibroblast Activation Protein Alpha expression in rheumatoid, myofibroblast like synoviocytes. Therapeutic aspects are also a part of the invention.
Abstract: A one- or two-dimensional arrangement of flow cells is provided, as part of an array of sample compartments, with at least one inlet and outlet for each sample compartment, formed by a base plate and a body, with an arrangement of spatial recesses corresponding to the (geometrical) arrangement of the sample compartments, combined with the base plate. The arrangement allows for supplying to or removing from the sample compartments, which can be arranged at a high quantity on a small base area, even very small amounts of samples or reagents.
Type:
Grant
Filed:
January 28, 2010
Date of Patent:
November 8, 2011
Assignee:
Bayer Technology Services GmbH
Inventors:
Eveline Schürmann-Mader, Andreas Peter Abel, Martin Andreas Bopp, Gert Ludwig Duveneck, Markus Ehrat, Gerhard Matthias Kresbach, Michael Pawlak, Nania Graciela Schärer-Hernández, Eginhard Schick
Abstract: An apparatus and a related method for performing particle agglutination reactions in at least one disposable probe tip are disclosed. The at least one probe tip includes a sample cavity for sample acquisition, at least one flanking cavity for the capture of particles by centrifugation or other means, a transition zone for the mixing of the sample with reagents for agglutination and a detection zone for the optical detection of particle agglutination. A mechanism may be attached to the probe tip for the controlled movement of fluids through the internal volume of the probe tip. The probe tip is particularly useful for the automation of high-throughput agglutination-type assays.
Abstract: An electronic biosensor arrangement (1), comprising a receiving region (2) for biological material, a sensor-electrode arrangement (3) with sensor electrodes (3a, 3b) which intermesh in a comb-like manner being associated therewith, with which sensor electrodes (3a, 3b) a measuring circuit (5) is connectable for measuring an electrical measurement parameter on the sensor electrodes (3a, 3b) and influenced by biological material, wherein the sensor electrodes (3a, 3b) form a plurality of sensor capacitors (Csensor,1 to Csensor,N), with which electronic switching means (S2,1 to S2,N) driven by a control logic (8) are associated for connecting to earth or to a voltage source carrying a measurement voltage (Vdrive), respectively, comprising a reference capacitor (Cref), to which a switching means (S1) driven by the control logic (8) and arranged for optionally connecting to earth or to the measurement voltage (Vdrive) is likewise assigned, wherein the capacitors (Cref, Csensor1?Csensor, N) are also combined to a
Type:
Grant
Filed:
May 15, 2007
Date of Patent:
October 25, 2011
Assignee:
Austrian Research Centers GmbH-ARC
Inventors:
Christa Nohammer, Daniel Paulo Wiese Meneses Rocha, Caspar Van Vroonhoven, Michael Johannes Vellekoop
Abstract: Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, the agents are directed electrokinetically to the sample collection assay device with no intermediate transfer steps. The agents are directed by creation of an electrokinetic potential well, which will effect their capture on to an assay device. Environmental agents such as biowarfare agents, pathogens, allergens or pollutants are collected autonomously on to the assay device, without any human intervention. The dielectric fluid medium, such as air, is sampled by electrokinetic propulsion with no moving parts or optionally, by transporting the dielectric fluid by a fan, pump or by breath.
Abstract: A method of detecting a presence of bio-molecules, or a concentration of the target bio-molecules using a field effect transistor, includes allowing a first sample including a first target bio-molecule to contact a sensing surface of the field effect transistor and measuring a change in an electric signal of the field effect transistor, the field effect transistor including a substrate, a source region and a drain region, the source region and the drain region formed apart from each other on the substrate, the source region and the drain region each doped to having an opposite polarity than a polarity of the substrate, a channel region disposed between the source region and the drain region and an insulating layer including the sensing surface, the insulating layer disposed on the channel region.
Abstract: A homogeneous immunoassay method and system for quantitative determination of total immunoglobulin E and specific antibody levels to a plurality of allergens, in which a relatively small sampling of blood is required. The method utilizes relatively small microparticles in aqueous suspension. The immunoassay procedure is an immunometric sandwich procedure preferably utilizing biotin-streptavidin signal amplification techniques and R-phycoerytherin fluorescent labels.
Abstract: An apparatus and method for automated picking of animal cell colonies is described. A picking head comprising a plurality of hollow pins is provided. The apparatus has an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations of the animal cell colonies to be picked. Picking an animal cell colony is performed by aligning each of the hollow pins in turn with a target animal cell colony location, introducing the hollow pin into the medium, and aspirating the animal cell colony into the hollow pin. In the case of an adherent colony, the distal end of the pin is forced into oscillation to detach the animal cell colony prior to aspiration. The animal cell colony is dispensed into a well plate by increasing pressure in the fluid conduit.
Type:
Grant
Filed:
April 12, 2006
Date of Patent:
October 11, 2011
Assignee:
Genetix Limited
Inventors:
Stephen Richmond, Christopher Mann, Andrew Board, Yonggang Jiang
Abstract: A homogeneous immunoassay method and system for quantitative determination of total immunoglobulin E and specific antibody levels to a plurality of allergens, in which a relatively small sampling of blood is required. The method utilizes relatively small microparticles in aqueous suspension. The immunoassay procedure is an immunometric sandwich procedure preferably utilizing biotin-streptavidin signal amplification techniques and R-phycoerytherin fluorescent labels.
Abstract: Methods and apparatus are disclosed for the measurement of lipocalin-2 in body fluids (including but not limited to blood, serum, plasma, urine, saliva, tear, etc.) by an assay such as an immunoassay or an immunotest for (1) the prediction of risk of future cardiovascular diseases; and (2) the determination of the likelihood that certain individuals will benefit to a greater or lesser extent from the use of certain treatments designed to prevent and/or treat cardiovascular diseases.
Type:
Grant
Filed:
April 30, 2008
Date of Patent:
October 4, 2011
Assignee:
Versitech Limited and R & C Biogenius Limited
Inventors:
Aimin Xu, Yu Wang, Reinhard Renneberg, George William Hunter Cautherley, Cangel Pui Yee Chan, Matthias Lehmann
Abstract: Methods and kits for determining sirolimus in a sample known or suspected to contain sirolimus are provided. The methods and kits according to the invention use one or more antibodies generated using a fragment of sirolimus, such as the northern fragment of sirolimus.
Abstract: The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
Type:
Grant
Filed:
June 8, 2006
Date of Patent:
September 20, 2011
Assignees:
The General Hospital Corporation, GPB Scientific, LLC
Inventors:
Lotien Richard Huang, Thomas A. Barber, Bruce L. Carvalho, Ravi Kapur, Paul Vernucci, Mehmet Toner, Zihua Wang
Abstract: The present invention relates to methods of detecting renal transplant rejection and other forms of renal damage. Protein markers or renal damage are provided, along with assays for detecting said markers. Also provided are methods for identifying markers of renal damage.
Abstract: The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements.
Type:
Grant
Filed:
July 1, 2008
Date of Patent:
September 13, 2011
Assignee:
Abbott Point of Care Inc.
Inventors:
Graham Davis, Imants R. Lauks, Chao Lin, Cary James Miller
Abstract: The present invention concerns antibodies specific for the C-terminus of human hepcidin, and related methods and kits for diagnosing and/or treating a disease condition characterized by non-physiological levels of hepcidin protein, including prohepcidin and fragments thereof, comprising obtaining a tissue or fluid sample from a subject; contacting the sample with an antibody or fragment thereof that specifically binds to a polypeptide corresponding to the amino acid sequence between and including amino acids 60 and 84, or, in another embodiment, amino acids 74 and 81, as aligned with the human pre-pro-hepcidin precursor protein, and quantifying the pro-hepcidin and/or mature hepcidin level using an assay based on binding of the antibody and the polypeptide; wherein the non-physiological level of prohepcidin/mature hepcidin is indicative of the disease condition.
Type:
Grant
Filed:
January 25, 2007
Date of Patent:
September 13, 2011
Inventors:
Hasan Kulaksiz, Cyril E. Geacintov, Alfred Jentzko
Abstract: Assay modules, preferably assay cartridges, are described as are reader apparatuses which may be used to control aspects of module operation. The modules preferably comprise a detection chamber with integrated electrodes that may be used for carrying out electrode induced luminescence measurements. Methods are described for immobilizing assay reagents in a controlled fashion on these electrodes and other surfaces. Assay modules and cartridges are also described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain preferred embodiments, these modules are adapted to receive and analyze a sample collected on an applicator stick.
Type:
Grant
Filed:
October 2, 2008
Date of Patent:
September 6, 2011
Assignee:
Meso Scale Technologies, LLC
Inventors:
Eli N. Glezer, Jonathan K. Leland, Mark A. Billadeau, Joseph M. Leginus, Bandele Jeffrey-Coker, Jeff D. Debad, Koustubh A. Phalnikar, Sriram Jambunathan