Abstract: Artificial high-fidelity three-dimensional human urogenital tract carcinomas are propagated under in vitro-microgravity conditions from carcinoma cells. Artificial high-fidelity three-dimensional human urogenital tract carcinomas are also propagated from a coculture of normal urogenital tract cells inoculated with carcinoma cells. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.

Abstract: A method for preparing aromatic substances having formula (I), wherein a substrate of formula (II) or an isomer of these precursors, also termed a substrate, in particular selected from those of formula (III), in which formulae R.sub.1 may be a --CH3, --CH2OH, --CHO, --COOH, or (a) radical, R.sub.2 may be a --H, --OH or --O--CH3 radical R.sub.3 may be a --H, --OH or --O--CH3 radical, and R.sub.2 and R.sub.3 may form a methylenedioxy bridge (b), is exposed to the activity of a lipoxygenase.

Abstract: Methods are presented for producing and purifying a variable fusion polypeptide which can be purified by affinity chromatography with the binding protein partner. The variable fusion polypeptide construct has tandem coupled segments containing one or more copies of a desired peptide linked to carbonic anhydrase as the purification binding protein. In the methods, the fusion protein is expressed in a recombinant host using a recombinant vector containing a gene encoding the fusion polypeptide. Then the expressed fusion polypeptide is purified by immobilized reversible inhibitor affinity chromatography. Finally, the purified fusion polypeptide is cleaved from the desired peptides by chemical or enzymatic means and the desired peptides purified with affinity chromatography.

Abstract: The Antibiotic Complex AB-023 and its components: Antibiotic AB-023a and ibiotic AB-023b are disclosed, which are obtained by the controlled aerobic culture of Streptomyces sp. NCIMB 40212 in an aqueous nutrient culture substrate. Antibiotics AB-023 display a biological activity against pathogen fungi which attack agricultural crops and man.

Abstract: Enhancement of the method and oral compositions for inhibiting adherence of Streptococcus pyogenes to pharyngeal and oral mucosa cells by treating these areas with a glycoconjugate comprising an oligosaccharide linked to a carrier. The oligosaccharide should have at least one fucose moiety or a galatose moiety which is free of digalactose and N-acetylneuraminyl lactose. The carrier may include albumins, starch or synthetic polymers.

Abstract: A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.

Abstract: It has been discovered that it is possible to administer truncated tissue factor (not having the transmembrane region) (tTF) in combination with factor VIIa (F VIIa) to treat bleeding disorders such as those resulting from hemophilia or cirrhosis of the liver. The tTF is administered to produce up to 10 .mu.g tTF/ml of plasma. The F VIIa is administered to produce levels of between 40 ng VIIa/ml and 4 .mu.g F VIIa/ml of plasma. The effective dosages of both tTF and VIIa are significantly and surprisingly less than the administration of either alone to stop bleeding. Examples demonstrate safety and efficacy in normal and hemophilic dogs.

Abstract: A process is provided for solubilizing coprecipitated metals. Metals in wastestreams are concentrated by treatment with an iron oxide coprecipitating agent. The coprecipitated metals are solubilized by contacting the coprecipitate with a bacterial culture of a Clostridium species ATCC 53464. The remobilized metals can then be recovered and recycled.

Abstract: The present invention pertains to a method for diagnosing for an aneurysm in a patient which comprises the steps of (a) explanting a skin section containing dermal fibroblast from the patient; (b) culturing the fibroblast to confluence in a culture medium; (c) incubating the cultured fibroblast with labeled proline to provide labeled procollagen in the culture medium; (d) separating the culture medium from the labeled procollagen and treating the labeled procollagen with a solution of protease inhibitor; (e) separating and purifying the labeled procollagen from the solution of protease inhibitor; (f) subjecting the labeled procollagen to protease digestion specific for non-collagenous proteins to form a collagenous mixture; (g) analyzing the collagenous mixture for the ratio of type I collagen to type III collagen; (h) analyzing a control collagenous mixture for the ratio of type I collagen to type III collagen; and (i) comparing the ratio of type I collagen to type III collagen in the collagenous mixture of

Abstract: A method for identifying soil microbial strains which may be bacterial degraders of pollutants comprising the steps of placing a concentration of a pollutant in a substantially closed container, placing the container in a sample of soil for a period of time ranging from one minute to several hours, retrieving the container, collecting the contents of the container, and microscopically determining the identity of the bacteria present. Different concentrations of the pollutant can be used to determine which bacteria respond to each concentration. The method can be used for characterizing a polluted site or for looking for naturally occurring biological degraders of the pollutant. Then bacteria identified as degraders of the pollutant and as chemotactically attracted to the pollutant are used to inoculate contaminated soil.

Abstract: The present invention relates to a compound which when administered to Leydig cells in the testes stimulates the production of androgen such as testosterone. This compound, when mixed with other known compounds, can produce testosterone levels in excess of those obtained by the administration of a maximal stimulatory dose of Lutenizing Hormone (LH).

Abstract: A rapid process for detecting pathogenic microorganisms in products for human consumption comprises contacting the microorganisms with a methylumbelliferone substrate. The substrate is hydrolyzed into methylumbelliferone by an enzyme given off by the microorganisms. Hydrolysis is accelerated by sodium lauryl sulfate, which renders the microorganisms more permeable to the substrate, the enzyme, or both. The methylumbelliferone is detected by its fluorescence, either in solution or on an agar medium supporting microcolonies formed from individual microorganisms.

Abstract: Disclosed is a treatment of hypertension with an effective amount of tannins extracted from Sapium sebiferum leaves, the tannins are 6-O-galloyl-D-glucose, corilagin, geraniin and 1,2,3,4,6-penta-O-galloyl-.beta.-D-glucose.

Abstract: A process for the detection of herbicides that inhibit acetolactate synthase (EC 4.1.3.18) by use of the enzyme, a suitable substrate (pyruvate, .alpha.-aceto-lactate, .alpha.-ketobutyrate, or .alpha.-aceto-.alpha.-hydroxybutyrate), and an oxygen-sensitive electrode is disclosed.

Abstract: A method for sealing the neck of a thermoplastic container to a thermoplastic cap superposed upon the neck and the cap having a lateral edge extending beyond the periphery of the neck using an ultrasonic welding apparatus includes an ultrasonic resonator having a domical internal cavity in its output end for reshaping the lateral edge of the cap in order to form a peripheral bead along the interface between the cap and the neck of the container.

Abstract: A machine for assembling a polystyrene spool by friction welding polystyrene end flanges (in the form of discs) to the polystyrene cylindrical sleeve or barrel of a spool, used for holding wire or cable wound thereon. The machine comprises a pair of coaxially spaced rotary mandrels, driven by respective motors, on which the end flange discs are first manually mounted, and a pair of cradle members for supporting a cylindrical sleeve in coaxial relation between the two mandrels. A control system is provided for initiating rotation of the mandrels with the end flanges, then causing inward movement of the mandrels toward the opposite ends of the sleeve with a predetermined air pressure to cause softening of a stepped shoulder on the end of the central polystyrene hub of the end flanges.

Abstract: A connection apparatus mainly comprises a gas-tight container one surface of which is made of a film, a gas supply source for filling the container with a gas at a predetermined pressure, and a heat source. Two substrates, at least one of which is flexible, are tightly supported on the film surface of the container in such a manner that electrodes or terminals formed on the both substrates confront with each other. The gas pressure in the container is increased to the predetermined value, thereby to tightly contact the electrodes formed on the both substrates with each other. Thereafter, the heat source is enabled to melt the electrodes and to electrically connect the two substrates with each other.