Abstract: Nutritional formulas comprising long-chain polyunsaturated fatty acids (LC-PUFAs) are provided, along with methods and devices for preparing and/or administering nutritional formulas. In some embodiments, a percentage of the LC-PUFAs in the nutritional formula are in the form of monoglycerides and/or free fatty acids. In some embodiments, the nutritional formulas do not comprise added lipase. Also provided are methods for providing nutrition to a subject, methods for improving fat absorption, methods for improving cognitive ability, methods for preventing chronic lung disease, and methods for reducing the length of time a patient requires total parenteral nutrition.

Abstract: Scalable biomaterial-based bioreactors are described. In one embodiment, the bioreactor may comprise perforated plates stacked such that the assembled bioreactor has the necessary manifolds and chambers to transport gas and liquids to a biomaterial contained within the bioreactor, and to remove the reaction products. In another embodiment, single use bioreactors are described. Methods of operating the bioreactors are also described.

Abstract: The invention, in various embodiments, provides systems, methods and solutions for perfusing an organ.

Abstract: A system and a method for treating biomass with a gas includes at least one conduit having at least one biomass inlet and at least one biomass outlet, at least one gas inlet and at least one gas outlet. The system further includes a transport unit configured to move the biomass through the conduit from the at least one biomass inlet to the at least one biomass outlet thereby defining a biomass transport direction. The system is configured such that gas flowing from the at least one gas inlet to the at least one gas outlet crosses the biomass transport direction.

Abstract: Systems and methods herein are directed towards the separation of biologic material to obtain a target cell volume and/or cell concentration for harvesting. The target volume and/or concentration of cells may be obtained through a single cycle via three chambers, or by repeated cycles through one or more chambers to dilute the digestive enzymes used in the process and concentrate the harvestable cell volume to a predetermined target.

Abstract: The present technology provides for a fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.

Abstract: A system and method for array system for cells, organoids and organs culture and testing. The system includes a disposable chips and systems with actuators, sensors, software/firmware and smart device App. The disposable includes standard well plates, custom well plates, T-flasks, microfluidic chips. The system includes vascular fluidics using gravity-driven flow and pneumatic flow, media, reagents, protein and collagen dispensers in wells or surfaces, manufacturing techniques for multi-layer chips and plates and culture system with gas and media control.

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating in a loop reactor which utilize substantially vertical flow zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

Abstract: A device and a process to count a number of colonies present in a set of samples. The colony counting device has a storage device with plural storage locations. A handling system is operative to convey samples to analyze from storage locations to an analysis area and from the analysis area to storage locations. An imaging device is operative to acquire a plurality of images of a sample to analyze, the plurality of images including all or a part of the analysis area. A processing unit is operative to implement, for each sample to analyze, a detection step to detect the presence of colonies by analyzing an image of the plurality of images of the sample and a determination step to determine the number of colonies present in the sample by counting the colonies whose presence have been detected during the detection step.

Abstract: Systems, devices, and methods for producing a tissue specimen and performing subsequent biomechanical testing thereof to quantify various biological/physiological properties and characteristics. The system may include a bioreactor device for producing the tissue specimen which includes a packaged/sealed seeding cup containing a scaffold in a sterile environment amenable to seeding with cells. After seeding, the cup may be stored in a standard laboratory culture tray for incubation. The produced tissue specimen may next be transferred to a testing instrument under control of a microcontroller which automatically performs a series of tests on the tissue and generates testing data communicated to an external electronic device which can operably interact with and control operation in part of the testing instrument via software. Tissue integrity in the instrument may be maintained by sterile fluid circulation and heating systems.

Abstract: An incubator (1) and its use for cultivating microorganisms and/or for carrying out a chemical reaction are provided. The incubator (1) has a sample vessel holder (3) for insertion of a sample vessel (4), a heating device (5) for changing a temperature in the sample vessel holder (3) and/or in a sample vessel (4) inserted into the sample vessel holder (3), and a sample-inactivating device (6) for inactivation of a sample (7) in a sample vessel (4) that is inserted into the sample vessel holder (3).

Abstract: A system that indicates the presence or absence of microorganisms in fluid food products. The system has a bottle for receiving sample to be tested. The bottle has a sensor that will monitor and detect changes in at least one sample parameter, but no additives that contain nutrients that support microbial growth. The bottle is placed in an incubator and the sensor in the bottle is monitored for changes. The incubator is programed so that, if the sensor detects that the value of the monitored parameter has reached a certain value, then the sample is determined to be positive for microbial growth.

Abstract: The disclosure relates to a composting device including a housing defining an interior with an access opening providing access to the interior, a composter bin located within the interior and in communication with the access opening, and a cover slidably mounted to the housing and slidable along a sliding path between opened and closed conditions to selectively open and close the access opening. A seal assembly comprising a seal depending from one of the cover or the housing and a sealing surface provided on the other of the cover or housing about the access opening. The seal arranged such that a space between the seal and the sealing surface decreases as the cover is slid from the opened to the closed condition, and the seal abuts and compresses against the sealing surface in the closed condition to seal the cover relative to the housing.

Abstract: A tissue processing system for dissociation of and release of cellular components from adipose tissue to prepare stromal vascular fraction includes a portable tissue processing unit for containing biological material including adipose tissue during enzymatic digestion processing and a digestion drive unit configured to receive the tissue processing unit for rotational processing of the tissue processing unit about an axis of rotation. The unit has an upright orientation and a reclined orientation in which the axis of rotation is at a reclined angle to horizontal relative to the upright orientation. The digestion drive unit is configured to receive and drive rotation of the tissue processing unit in the reclined orientation. Methods for processing biological material including adipose tissue include enzymatically digesting adipose tissue with rotation of a tissue processing unit around an axis of rotation in a reclined orientation.

Abstract: The present invention provides for a fully integrated microfluidic system capable of producing single-dose amounts of biotherapeutics at the point-of-care wherein protein production, purification and product harvest are all integrated as a single microfluidic device which is portable and capable of continuous-flow production of biotherapeutics at the microscale using a cell-free reaction system.

Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

Abstract: Vial assemblies comprise a tubular body and a cap, the cap including a first portion configured to abut a lip of an open end of the tubular body, a threaded portion configured to couple to threading on an internal surface of the tubular body, and a second portion protruding from the threaded portion and extending into a cavity of the tubular body. Methods for storing and removing frozen samples from such vial assemblies are also described.

Abstract: The present invention relates to cell free protein manufacturing, and more particularly, for integrating machine learning into a portable cell-free bioprocessing system for producing proteins with increased and consistent purity, potency and quality wherein such proteins are prepared on-demand and for point-of-care delivery.

Abstract: A cell sheet transfer device and a cell sheet processing equipment. The cell sheet transfer device includes a first tubular body; a second tubular body at one end of the first tubular body and connected thereto; and a cell shovel at the end of the first tubular body and on one side of the second tubular body. A free end of the cell shovel is beyond a free end of the second shovel body.

Abstract: Provided herein are systems and method for measuring cell stiffness. In particular, provided herein are microelectrode configuration and systems for measuring platelet deformation and stiffness.