Patents Examined by Paul C. Martin
  • Patent number: 11426680
    Abstract: The present invention relates generally to a method of reducing the level of at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in a solution comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and von Willebrand factor (VWF), the method comprising: (i) passing a feedstock comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF through a hydrophobic charge-induction chromatographic resin under conditions selected such that at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) present in the feedstock is bound to the resin; and (ii) recovering a solution comprising the at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF which passes through the resin, wherein the concentration of the at least one protein selected from the group consisting of pl
    Type: Grant
    Filed: December 10, 2018
    Date of Patent: August 30, 2022
    Assignee: CSL BEHRING GmbH
    Inventors: Hung Pham, Jeffrey Michael Hey, Darren Nguy
  • Patent number: 11414645
    Abstract: The present invention relates to a cell culture support comprising a substrate and a polymeric blend layer bound to the substrate. The polymeric blend layer comprises at least one thermoresponsive polymer and at least one coupling agent. The coupling agent is a non-protein coupling agent that has functional thiol, ester, epoxy, or aldehyde groups. The cell culture support further includes cells supported by the polymeric blend layer, wherein the thermoresponsive polymer provides for temperature induced detachment of the cells and/or cell sheets.
    Type: Grant
    Filed: January 4, 2019
    Date of Patent: August 16, 2022
    Assignee: The University of Akron
    Inventors: Abdullah Alghunaim, Bi-min Zhang Newby
  • Patent number: 11352656
    Abstract: The present invention is related to compositions comprising clarified limulus amebocyte lysate (LAL), wherein the LAL is substantially free of coagulogen and methods of making such compositions. The invention further relates to a method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising: (a) contacting the sample with a reagent comprising clarified LAL and a chromogenic substrate; and (b) measuring a chromogenic effect resulting from a change in the chromogenic substrate in the presence of endotoxin in the sample, wherein the LAL is substantially free of coagulogen. The invention also relates to kits comprising clarified LAL substantially free of coagulogen, and methods of making such.
    Type: Grant
    Filed: January 11, 2018
    Date of Patent: June 7, 2022
    Inventors: Candice Stumbaugh, Leben Tadesse, Karen Lin
  • Patent number: 11318225
    Abstract: Thin parylene C membranes having smooth front sides and ultrathin regions (e.g., 0.01 ?m to 5 ?m thick) interspersed with thicker regions are disclosed. The back sides of the membranes can be rough compared with the smooth front sides. The membranes can be used in vitro to grow monolayers of cells in a laboratory or in vivo as surgically implantable growth layers, such as to replace the Bruch's membrane in the eye. The thin regions of parylene are semipermeable to allow for proteins in serum to pass through, and the thick regions give mechanical support for handling by a surgeon. The smooth front side allows for monolayer cell growth, and the rough back side helps prevents cells from attaching there.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: May 3, 2022
    Assignees: California Institute of Technology, University of Southern California
    Inventors: Yu-Chong Tai, Bo Lu, Mark Humayun
  • Patent number: 11319342
    Abstract: Embodiments of the invention relate generally to protein extraction and, more generally, to bone protein extraction methods that do not require demineralization. In one embodiment, the invention provides a method comprising: mixing a bone sample and a quantity of an extraction buffer comprising: ammonium phosphate dibasic; or ammonium phosphate dibasic and ammonium bicarbonate; or ammonium phosphate dibasic, ammonium bicarbonate, and guanidine HCl; or sodium phosphate dibasic and sodium bicarbonate; or sodium phosphate dibasic, sodium bicarbonate, and guanidine HCl; or potassium phosphate dibasic and potassium bicarbonate; or potassium phosphate dibasic, potassium bicarbonate, and guanidine HCl; and incubating the bone sample/extraction buffer mixture.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: May 3, 2022
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Timothy Paul Cleland, Deepak Vashishth
  • Patent number: 11291403
    Abstract: The present disclosure provides methods for determining an etiology of upper and/or lower eyelid puffiness by examining a subject with squinted eyes; and determining if upper and/or lower eyelid puffiness does not improve, improves, partially improves, or worsens, wherein the etiology of the upper and/or lower eyelid puffiness is diagnosed to be anterior to the orbicularis oculi muscle if the puffiness does not improve, wherein the etiology of the upper and/or lower eyelid puffiness is determined to be posterior to the orbicularis oculi muscle if the puffiness improves, wherein the etiology of the upper and/or lower eyelid puffiness is determined to be anterior and posterior to the orbicularis oculi muscle if the puffiness partially improves, or wherein the puffiness is determined to be secondary to hypertrophy of the orbicularis muscle or if the puffiness worsens.
    Type: Grant
    Filed: April 3, 2020
    Date of Patent: April 5, 2022
    Assignee: STANDARD OF CARE CORPORATION
    Inventors: Iliana E. Sweis, Bryan C. Cressey
  • Patent number: 11285100
    Abstract: The present application relates to a freeze-dried polymer composition containing chitosan and at least one lyoprotectant, a process for preparing a freeze-dried composition containing chitosan and at least one lyoprotectant and the use of a reconstituted freeze-dried chitosan composition to prepare implants for tissue repair.
    Type: Grant
    Filed: February 20, 2015
    Date of Patent: March 29, 2022
    Assignee: Ortho Regenerative Technologies Inc.
    Inventors: Anik Chevrier, Michael D. Buschmann, Daniel Veilleux, Caroline Hoemann, Marc Lavertu
  • Patent number: 11285179
    Abstract: The invention surprisingly found that Lactobacillus plantarum subsp. plantarum PS128 provides an advantageous effect in treatment or prevention of tic disorders and basal ganglia disorders. Accordingly, the invention provides a method of treating or preventing a movement disorder in a subject, comprising administering to a subject an effective amount of cells of a Lactobacillus plantarum subsp. plantarum PS128, which is deposited under DSMZ Accession No. DSM 28632.
    Type: Grant
    Filed: July 19, 2016
    Date of Patent: March 29, 2022
    Assignees: BENED BIOMEDICAL CO., LTD., ASIAN PROBIOTICS AND PREBIOTICS CORPORATION
    Inventors: Ying-Chieh Tsai, Chih-Chieh Hsu, Jian-Fu Liao, Yun-Fang Cheng, Shu-Ting You
  • Patent number: 11286448
    Abstract: An enzymatic processing plant for continuous flow-based enzymatic processing of organic molecules. The enzymatic processing plant including an enzymatic processing area, wherein the enzymatic processing area includes a turbulence-generating pipe with a repeatedly changing centre-line and/or a repeatedly changing cross-section, for generating turbulence to mix a reaction mixture and prevent sedimentation of particles as the reaction mixture is flowing through the turbulence-generating pipe. The enzymatic processing plant and the enzymatic processing area are arranged such that the reaction mixture is subjected to turbulence within the enzymatic processing area for a reaction time of 15 minutes or more.
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: March 29, 2022
    Assignee: ALKYMAR AS
    Inventors: Olav Asle Djupevåg, Torstein Frantzen
  • Patent number: 11225636
    Abstract: The present invention relates to a method and system for aseptic material transfer from a storage container to a bioreactor. More precisely the invention relates to a method and system for aseptically transferring dry material, such as microcarriers for cell cultivation, to a bioreactor, comprising transferring microcarriers from a first container housing said microcarriers to a bioreactor for cell cultivation via r transfer tubing connecting the first container to the bioreactor, wherein the transfer is accomplished with pressurized air or gas supplied to the container.
    Type: Grant
    Filed: May 16, 2016
    Date of Patent: January 18, 2022
    Assignee: Cytiva Sweden AB
    Inventors: Bjorn Johan Lundgren, Emmanuel Macedo, Ann-Christin Magnusson, Lena Maria Sandberg
  • Patent number: 11191815
    Abstract: A closed-loop insulin delivery system is described. More particularly, the presently disclosed insulin delivery system can comprise glucose-responsive vesicles that release insulin in response to hypoxia triggered by enzymatic reduction of glucose. In addition or as an alternative to insulin, the delivery system can release other diabetes treatment agents, such as non-insulin-based diabetes treatment agents. The vesicles can be prepared from a hypoxia sensitive polymer, such as a hypoxia-sensitive hyaluronic acid (HS-HA). The HS-HA can comprise hydrophobic groups that can be reduced in hypoxic environments to form hydrophilic groups. The vesicles can be loaded into microneedles and microneedle array patches for use in the treatment of diabetes or to otherwise regulate blood glucose levels in subjects in need of such treatment.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: December 7, 2021
    Assignee: North Carolina State University
    Inventors: Zhen Gu, Jicheng Yu
  • Patent number: 11191776
    Abstract: The present invention provides solid formulations of hyaluronic acid (HA) that are resistant to degradation into low molecular weight species of HA and yet can be readily dissolved in water. The compositions comprise sodium hyaluronate, cyclo-dextrin, sodium benzoate, and potassium sorbate, wherein said composition is in powder form.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: December 7, 2021
    Inventor: Donald Wayne Jessup
  • Patent number: 11155778
    Abstract: Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: October 26, 2021
    Assignee: Wilson Wolf Manufacturing
    Inventors: Daniel P. Welch, John R. Wilson
  • Patent number: 11079398
    Abstract: The invention discloses a method for measurement of peptidic degradation products of a proteolytic cascade in biological samples, especially blood samples, wherein the sample is incubated until a steady state equilibrium is reached for at least one peptidic degradation product involved in said proteolytic cascade and wherein said at least one peptidic degradation product in steady state equilibrium of the proteolytic cascade is quantified in the sample.
    Type: Grant
    Filed: May 5, 2017
    Date of Patent: August 3, 2021
    Assignee: ATTOQUANT DIAGNOSTICS GMBH
    Inventors: Marko Poglitsch, Cornelia Schwager, Hans Loibner, Manfred Schuster
  • Patent number: 11078516
    Abstract: Orally administered physiologically acceptable anti-biofilm compositions comprising enzymes and if desired additional components such as antimicrobials, antibiotics, antifungals, herbals, chelating agents, lactoferrin and related compounds, minerals, surfactants, binders, and fillers useful for the inhibition and treatment of gastrointestinal biofilms in humans. Physiologically acceptable anti-biofilm compositions containing these enzymes are useful in the inhibition, reduction and/or treatment of gastrointestinal biofilm infections, and associated systemic symptoms caused by biofilms associated microorganisms within the gastrointestinal tract. Methods of identification, preparation and use of such physiologically acceptable anti-biofilm compositions are also provided.
    Type: Grant
    Filed: May 14, 2018
    Date of Patent: August 3, 2021
    Assignee: Prothera, Inc.
    Inventor: Stephen Francis Olmstead
  • Patent number: 11071707
    Abstract: The present invention is concerned with a composition for repairing human skin. Specifically, the composition has a DNA repair enzyme and at least one phycobiliprotein. The DNA repair enzyme is a photolyase, and the photolyase and/or the phycobiliprotein is encapsulated by liposomes. The composition is effective in repairing damage to human skin as a result of environment daily stress, sun exposure, or premature-aging.
    Type: Grant
    Filed: July 20, 2016
    Date of Patent: July 27, 2021
    Assignee: ALGAKTIV, S.L.
    Inventors: Eugenia Ruiz Canovas, María Teresa López Cerro, Derek Georges Latil de Ros, Olga Durany Turk, Jordi Segura de Yebra, Jaume Mercadé Roca
  • Patent number: 11035763
    Abstract: A method and a kit are provided to produce a biological clear specimen within a short time and with a wide variety of biological specimens. The method includes the following steps: (1) treating a material to be cleared with an aqueous fixing solution containing paraformaldehyde or the like, a nonionic surfactant, an alkali, and optionally a buffering agent; (2) treating the material from step (1) or a conventionally fixed material with an aqueous clearing accelerator solution containing a nonionic surfactant and an alkali; and (3) treating the material produced in step (1) or (2) with an aqueous preservation solution containing a nonionic surfactant and a polyhydric alcohol. In each of the steps, the nonionic surfactant is present at a concentration of 1% or more. The kit includes solutions used in each of the steps of the method.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: June 15, 2021
    Assignee: KANAZAWA MEDICAL UNIVERSITY
    Inventors: Toshihisa Hatta, Maimi Uchishiba, Nobuaki Higashi, Hiroki Shimada, Eriko Shimamura
  • Patent number: 11016104
    Abstract: A therapeutic composition for the treatment of the symptoms of neurological and mental health disorders, such as Alzheimer's, bipolar disorder, obsessive compulsive disorder, and oppositional defiant disorder, and the method for preparing the therapeutic agents is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using fecal chymotrypsin level as an indicator of the presence of neurological and mental health disorders, such as Alzheimer's, bipolar disorder, obsessive compulsive disorder, and oppositional defiant disorder, or the likelihood of an individual to develop these disorders is disclosed.
    Type: Grant
    Filed: July 1, 2009
    Date of Patent: May 25, 2021
    Assignee: CUREMARK, LLC
    Inventor: Joan M. Fallon
  • Patent number: 10993434
    Abstract: The present invention relates to an exopolysaccharide derived from Pseudoalteromonas sp. strain CY01 (KCTC 12867BP) which is a novel strain living in the polar regions, and to a composition for cryoprotection of cells, which contains the exopolysaccharide. The exopolysaccharide of the present invention has an excellent ability to cryoprotect cells, and shows no cytotoxicity. Thus, the inventive exopolysaccharide can substitute for conventional cryoprotective agents that show cytotoxicity when used at high concentrations.
    Type: Grant
    Filed: December 8, 2017
    Date of Patent: May 4, 2021
    Assignee: Korea Institute of Ocean Science and Technology
    Inventors: Joung Han Yim, Il-Chan Kim, Se Jong Han, Ui Joung Youn, Hong Kum Lee, Sung Jin Kim, Pil-Sung Kang, Jung Eun Kim, Tai Kyoung Kim, Ha Ju Park, Jin Haeng Song, Min Ju Kim, Ju Mi Hong, Dong-Gyu Jo
  • Patent number: 10976258
    Abstract: The invention relates to a porous planar cell capture system for use in determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using such a cell capture system. The cell capture system contains a fluid permeable, planar membrane adopted to retain cells thereon, a fluid permeable support member that supports the membrane, and an optional register associated with the membrane.
    Type: Grant
    Filed: February 25, 2019
    Date of Patent: April 13, 2021
    Assignees: Charles River Laboratories, Inc., ReaMetrix Inc.
    Inventors: Norman R. Wainwright, Bala S. Manian, Eric Stimpson, Brian J. Kolonia, Robert K. Kolonia