Patents Examined by Paul C. Martin
  • Patent number: 10704065
    Abstract: There is described a method for producing polymeric 3-hydroxypropionamide (3HP amide), the method comprising: culturing an Acetobacter lovaniensis-bacterium in a growth medium containing phosphate and ammonium, wherein culturing of the bacterium produces polymeric 3HP amide. The polymeric 3HP amide may then be hydrolysed to 3HP amide or converted to other compounds of interest.
    Type: Grant
    Filed: January 30, 2017
    Date of Patent: July 7, 2020
    Inventor: Irene Finnegan
  • Patent number: 10684286
    Abstract: The invention provides a method for analyzing a protamine sample for the presence or amount of at least one nucleotidic impurity. The invention also provides a method for the quantitation of peptides in a sample of protamine comprising four major peptides and at least one related impurity.
    Type: Grant
    Filed: April 6, 2017
    Date of Patent: June 16, 2020
    Assignee: Fresenius Kabi USA, LLC
    Inventors: Pearle Torralba, David Jeschke, Kamlesh Patel
  • Patent number: 10655158
    Abstract: Disclosed is a bacterial counting method, comprising the following steps, S1: preparing a polyaniline/bacterial composite film on the surface of a glassy carbon electrode by electric polymerization; S2: drawing the standard curve of the polyaniline/bacterial composite film modified electrode; and S3: determining the bacterial concentration of the bacteria solution sample to be tested according to the standard curve obtained in step S2. The method does not require the cultivation of the bacteria in the implementation process, such that same takes a short time and is easy to operate; the method uses aniline as the main reagent, and the consumption of the solution to be tested is small, such that the testing cost is low and the equipment is simple; the method has the advantages of a good repeatability and a wide detection linear range; and the method is an easy to operate, short time-consuming, and low cost bacterial counting method.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: May 19, 2020
    Assignee: Jiangsu University
    Inventors: Xiaobo Zou, Zhihua Li, Jiyong Shi, Xiaowei Huang, Xucheng Zhou, Xuetao Hu
  • Patent number: 10626069
    Abstract: The invention pertains to a process for preparing methyl lactate including the steps of: bringing an aqueous liquid comprising lactic acid, methanol, and at least 5 wt. % of a dissolved chloride salt selected from magnesium chloride, calcium chloride, and zinc chloride to reaction conditions, thereby obtaining methyl lactate, wherein an extractant is provided to the reaction mixture before, during, and/or after formation of methyl lactate; subjecting the reaction mixture to a liquid-liquid separation step wherein an organic phase comprising methyl lactate and extractant is separated from an aqueous phase comprising dissolved chloride salt. The extractant preferably includes one or more compounds selected from C5+ ketones and C3-C10 ethers, in particular C5-C8 ketones, more in particular methyl isobutyl ketone. It has been found that the process according to the invention makes it possible to manufacture methyl lactate efficiently and in high yield.
    Type: Grant
    Filed: February 13, 2014
    Date of Patent: April 21, 2020
    Assignee: PURAC BIOCHEM B.V.
    Inventors: Jan Van Krieken, André Banier De Haan, Jan Van Breugel
  • Patent number: 10564148
    Abstract: Disclosed herein are media for culture of cells, tissues, and/or organs. The media formulations disclosed herein can be used to support growth, viability, and/or function of one or more than one cell type, tissue, or organ. In some embodiments, one or more cell types, tissues, organ devices, and/or organs are contacted with a disclosed culture medium under conditions sufficient to support growth, viability, and/or function of the cell types, tissues, and/or organs. The disclosed media can be used in methods of culturing multiple cell types, and in some examples, is used in a platform device including one or more organ devices, for example, by circulating the medium through the one or more organ devices in the platform.
    Type: Grant
    Filed: September 24, 2015
    Date of Patent: February 18, 2020
    Assignees: Triad National Security, LLC, CFD Research Corporation
    Inventors: Rashi Iyer, Jennifer F. Harris, Jen-Huang Huang, Pulak Nath, Andrzej Przekwas
  • Patent number: 10550415
    Abstract: The present invention relates to a system for production of ATP. This system is comprised of a support and one or more enzymes coupled to that support which are capable of collectively producing ATP from glucose or fructose metabolism. The present invention is additionally directed to a device, which includes the system, and to a method for carrying out a reaction involving the conversion of ATP to ADP using the system.
    Type: Grant
    Filed: October 16, 2008
    Date of Patent: February 4, 2020
    Assignee: Cornell Research Foundation, Inc.
    Inventor: Alexander Travis
  • Patent number: 10533208
    Abstract: Methods are provided for the prognosis, diagnosis and treatment of various pathological states, including cancer, chemotherapy resistance and dementia associated with Alzheimer's disease. The methods provided herein are based on the discovery that various proteins with a high level of sialylation are shown herein to be associated with disease states, such as, cancer, chemotherapy resistance and dementia associated with Alzheimer's disease. Such methods provide a lysosomal exocytosis activity profile comprising one or more values representing lysosomal exocytosis activity. Also provided herein, is the discovery that low lysosomal sialidase activity is associated with various pathological states. Thus, the methods also provide a lysosomal sialidase activity profile, comprising one or more values representing lysosomal sialidase activity. A lysosomal sialidase activity profile is one example of a lysosomal exocytosis activity profile.
    Type: Grant
    Filed: November 9, 2017
    Date of Patent: January 14, 2020
    Inventors: Ida Annunziata, Alessandra D'Azzo
  • Patent number: 10501773
    Abstract: In some embodiments, the invention provides methods for detecting and/or classifying an anticoagulant at a therapeutically relevant amount or higher in a patient, including subjecting a sample of a control blood component (known not to contain the anticoagulant) to a clotting assay in the presence of a Factor Xa reagent to obtain a control clotting measurement; and subjecting a sample of a blood component from a patient suspected of having the anticoagulant to the clotting assay in the presence of the Factor Xa reagent to obtain a patient clotting measurement, wherein the patient clotting measurement sample greater than the control clotting measurement indicates the presence of the anticoagulant at a therapeutically relevant amount or higher in the patient. In some embodiments, the invention includes methods for classifying an anticoagulant as an anti-Factor Xa or a direct thrombin inhibitor anticoagulant using a clotting assay in the presence of an ecarin reagent.
    Type: Grant
    Filed: July 30, 2015
    Date of Patent: December 10, 2019
    Assignee: Haemonetics Corporation
    Inventors: Fowzia S. Zaman, Marc Doubleday
  • Patent number: 10494601
    Abstract: The present invention relates to a method for inducing osteogenic differentiation of mesenchymal stem cells and, more particularly, to a short-time osteogenic differentiation method of culturing cells using a porous membrane and a biodegradable synthetic biogel, whereby the cells do not contact a cell culture container. The present invention can significantly shorten the induction period of osteogenic differentiation, compared to the conventional osteogenic differentiation method, and has an effect of the cells being easily separable after differentiation as well.
    Type: Grant
    Filed: September 15, 2015
    Date of Patent: December 3, 2019
    Assignee: CEFO CO., LTD
    Inventors: Hyun Sook Park, Sun Ray Lee, Ji Won Yang, Seol Chu, Hyun Jung Mo
  • Patent number: 10479969
    Abstract: Bioreactors and methods for cultivating microalgae under heterotrophic growth conditions using fruit wastewater as a carbon source are provided herein. The heterotrophic growth conditions include low irradiance of light sufficient to initiate light-activated metabolism, but not photosynthesis. The methods provide a cost effective and environmentally friendly solution for converting wastewater from dried fruit and wine industries into products like biofuels.
    Type: Grant
    Filed: October 11, 2011
    Date of Patent: November 19, 2019
    Inventors: Jane Kim, Chung-Soon Im
  • Patent number: 10450237
    Abstract: The present invention relates to a novel phosphate solubilizing strain of Penicillium bilaiae, which is a hybrid of Penicillium bilaiae ATCC 22348 and Penicillium bilaiae V08/021001, as well as compositions and methods for using the novel phosphate solubilizing strain to increase the availability of phosphorous for plant uptake from soil.
    Type: Grant
    Filed: August 7, 2017
    Date of Patent: October 22, 2019
    Inventors: David Greenshields, Shelagh Steckler, Kari Priest, Caressa Caldwell, Michael Frodyma
  • Patent number: 10421949
    Abstract: Provided herein are methods of culturing an adherent mammalian cell in a shake tube using a plurality of microcarriers and batch re-feed perfusion, and various methods that utilize these culturing methods.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: September 24, 2019
    Assignee: Genzyme Corporation
    Inventors: Jianguo Yang, Yang Yang
  • Patent number: 10364447
    Abstract: The present invention provides a method for producing ?-3 polyunsaturated fatty acids by culturing specific myxobacterial stains and myxobacterial strains suitable for said method.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: July 30, 2019
    Assignee: Universitat Des Saarlandes
    Inventors: Ernst Roemer, Rolf Müller, Ronald O. Garcia, Mark Stadler, Dominik Pistorius, Alexander Brachmann
  • Patent number: 10350248
    Abstract: Provided are uses of Bacteroides in preparation of a medicament. The medicament is for use in treatment or prevention of obesity and obesity-related diseases. Also provided are a pharmaceutical composition, a medicament, a food product, and an animal feed that comprise Bacteroides, uses thereof in treatment or prevention of obesity and obesity-related diseases, and a method for treatment or prevention of obesity and obesity-related diseases.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: July 16, 2019
    Inventors: Guang Ning, Jie Hong, Weiqing Wang, Ruixin Liu, Jiqiu Wang
  • Patent number: 10330688
    Abstract: This invention provides methods and biomarkers for diagnosing autism by identifying cellular metabolites differentially produced in autistic patient samples versus non-autistic controls. Methods for identifying a unique profile of metabolites present or secreted in brain tissue, cerebrospinal fluid, plasma, or biofluids of autistic samples are described herein. The individual metabolites or a pattern of secreted metabolites provides metabolic signatures of autism, which can be used to provide a diagnosis thereof.
    Type: Grant
    Filed: December 7, 2016
    Date of Patent: June 25, 2019
    Assignees: Wisconsin Alumni Research Foundation, Stemina Biomarker Discovery, Inc.
    Inventors: Gabriela Gebrin Cezar, Alan M. Smith
  • Patent number: 10324100
    Abstract: The present invention provides methods for quantifying an amount of plasmalogens in samples with high accuracy in an easy, convenient and inexpensive manner by using a hydrolysis processing to samples and a lipid extraction followed by the measurement using High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) or Mass Spectrometer (MS), a fluorescence plate reader or a plate reader. The present invention also relates to a method for examining a subject by using the above method, a biomarker for disease detection, a method for using the biomarker for the disease detection, as well as a kit for the disease detection.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: June 18, 2019
    Assignee: Institute of Rheological Functions of Food
    Inventors: Takehiko Fujino, Shiro Mawatari
  • Patent number: 10324036
    Abstract: The invention relates to a porous planar cell capture system for use in determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using such a cell capture system. The cell capture system contains a fluid permeable, planar membrane adopted to retain cells thereon, a fluid permeable support member that supports the membrane, and an optional register associated with the membrane.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: June 18, 2019
    Assignees: Charles River Laboratories, Inc., ReaMetrix Inc.
    Inventors: Norman R. Wainwright, Bala S. Manian, Eric Stimpson, Brian J. Kolonia, Robert K. Kolonia
  • Patent number: 10323226
    Abstract: A bacteria starter material for use in biomass generators without causing jamming of the feeder mechanism, particularly under high temperature and/or high humidity conditions, the starter material comprising one or more species of waste-decomposing bacteria, sucrose, and a soy based protein. Preferably, DI-PAC® sugar (sucrose and maltodextrin) and powdered KIKKOMAN® Soy Sauce are used as the sugar and protein ingredients. The starter material may also comprise a dried bran culture comprising Pseudomonas. The starter material ingredients are blended together and formed into a tablet or pellet having a particular shape, size, and hardness characteristics suitable for use in the feeder mechanism of a biomass generator.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: June 18, 2019
    Assignee: NCH Corporation
    Inventors: Judith G. Pruitt, Robert C. Pearce, III, Daniel Aberle, Charles J. Greenwald, Gabriel F. K. Everett
  • Patent number: 10323264
    Abstract: The invention provides a method for producing methacrylyl-CoA that converts 3-hydroxyisobutyryl-CoA into methacrylyl-CoA using an enzyme having dehydratase activity as a method for producing methacrylyl-CoA using an enzyme catalyst. In this production method, conversion rate of 3-hydroxyisobutyryl-CoA into methacrylyl-CoA by the enzyme having dehydratase activity is 50% or higher. In this production method, furthermore, the enzyme having dehydratase activity derives from a microorganism belonging to the genus Pseudomonas or Rhodococcus.
    Type: Grant
    Filed: July 25, 2014
    Date of Patent: June 18, 2019
    Assignee: Mitsubishi Chemical Corporation
    Inventors: Eiji Sato, Fujio Yu, Eiji Nakajima, Michiko Yamazaki, Wataru Mizunashi
  • Patent number: 10323217
    Abstract: The disclosure concerns a detergent composition comprising one or more anionic surfactants; an enzyme selected from the group consisting of: a protease, a lipase, a cutinase, an amylase, a carbohydrase, a cellulase, a pectinase, a mannanase, an arabinase, a galactanase, a xylanase, and an oxidase; and a deoxyribonuclease (DNase), as well as methods for washing a textile.
    Type: Grant
    Filed: December 9, 2013
    Date of Patent: June 18, 2019
    Assignee: Novozymes A/S
    Inventors: Klaus Gori, Lilian Eva Tang Baltsen, Marie Allesen-Holm