Abstract: There is disclosed a method for preparation of substrate for use in anaerobic digestion of organic waste. The method can be executed in a substrate pretreatment system, the method comprising: loading of a substrate into the substrate pretreatment system, mixing the substrate with liquid; heating the substrate and liquid to a pre-determined processing temperature; exposing the mixture to radiation; transferring the processed substrate to a bioreactor, during the heating, the method further comprises: degassing the mixture using vacuum extraction; and circulating the mixture past an ultrasonic hydrodynamic radiator.

Abstract: Disclosed are products having animal tissue packed within the lumen of a device such as a needle cannula and being therein impregnated with a cryopreservation medium. The needle cannula or other device can be received in a capsule and/or other container, which in some forms can contain additional amounts of the cryopreservation medium occurring external of the lumen. Methods of use of the products are also described and can include ejecting the animal tissue from the lumen of device using pressurized liquid passed through the lumen, potentially to deliver the animal tissue directly into a patient. Methods of manufacture of the products are also described.

Abstract: The present invention generally relates to the field of preventing and/or treating inflammatory and infectious disorders, in particular by boosting the endogenous antimicrobial defences. One embodiment of the present invention is the use of non-replicating L. johnsonii La1 NCC533 (deposit number CNCM I-1225) for use in the treatment or prevention of disorders related to the immune system including infections.

Abstract: Provided is a microorganism culture sheet having an excellent efficiency for collection of environmental microorganisms and a method for measuring the environmental microorganisms with an excellent collection efficiency. Provided is a microorganism culture sheet including a base sheet, a dry culture layer formed on the base sheet, and a cover sheet covering the dry culture layer, wherein an adhesive layer and a release film are laminated on the inner surface of the cover sheet. Because the environmental microorganisms are collected using the adhesive layer, an excellent collection efficiency is provided.

Abstract: A device and a method are provided for isolating a fraction in a biological sample. The fraction is bound to solid phase substrate to define a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein to capture a desired fraction of the biological sample. A force is provided that is generally perpendicular to gravity. The force is movable between a first position adjacent the input zone multiple other positions adjacent various purification, protein analysis, separation and extraction zones. The force captures the fraction-bound solid phase substrate and the fraction-bound solid phase substrate moves from the input zone to the other zones to perform a multi-step assay on the isolated fraction within the device.

Abstract: A system and method for converting biomass with no chemical pretreatment is disclosed. Combination of a microbial system and the use of mechanical disruption during fermentation may help achieve high conversion rate without the extra cost and undesirable by-products typically associated with the pretreatment process.

Abstract: A fluidic device (1) has a culture chamber (10) configured to house a 3D culture matrix (2) comprising a culture of microorganisms (6). A concentration gradient of a test substance is established over the 3D culture matrix (2) by providing respective fluid flows at different end portions (12, 14) of the culture chamber (10) and comprising different concentrations of the test substance. The response of the microorganisms (6) to the test substance is determined based on the position of a border zone (5) in the 3D culture matrix (2).

Abstract: Screening methods as well as systems and kits for identifying inhibitors of DNA-independent, histone H4-dependent activation of PARP-1 are provided. The methods comprise screening molecules for their capacity to inhibit the activation and/or biologic activity of PARP-1, as measured by poly(ADP)-ribose production from nicotinamide adenine dinucleotide. PARP-1 inhibitors identified through the screening methods may be used to treat cancer in which PARP-1 activation or biologic activity plays a role.

Abstract: The present invention relates to a process for production of fermentation products, including bioethanol by non-pressurized pre-treatment, enzymatic hydrolysis and fermentation of waste fractions containing mono- and/or polysaccharides, having a relatively high dry matter content. The process in its entirety, i.e. from non-pressurized pre-treatment over enzymatic hydrolysis and fermentation to sorting of fermentable and non-fermentable solids can be processed at a relatively high dry matter content in a single vessel or similar device using free fall mixing for the mechanical processing of the waste fraction.

Abstract: Oral administration of ALV003 can protect celiac disease patients and patients otherwise suffering from gluten-intolerance from the harmful effects of ingesting food containing gluten.

Abstract: Methodologies, formulations, and kits suitable for decontaminating environments containing bacterial spores by degrading the spores. Formulations contain papain and at least one germinant. Methods for killing bacterial spores include contacting the spores with the formulation for a duration sufficient to initiate germination of the bacterial spores. The spores are then killed with the papain in the formulation, or are rendered by the papain susceptible to being killed by a reagent.

Abstract: The present invention relates to apparatus, methods, and applications for treating wastewater, and more particularly to biological processes for removing pollutants from wastewater. This invention further relates to apparatus and methods for growing microbes on-site at a wastewater treatment facility, and for economically inoculating sufficient microbes to solve various treatment problems rapidly.

Abstract: The invention relates to methods for directing differentiation of stem cells comprising graphene. In additional embodiments, the invention relates to methods for repairing and improving bone tissue functions comprising accelerating differentiation in stem cell growth by exposing stem cells to graphene and transplanting the graphene with the exposed stem cells in the tissue at the site of repair.

Abstract: Clostridium difficile disease involves a range of clinical presentations ranging from mild to self-limiting diarrhea to life-threatening pseudomembranous colitis and megacolon. Cases of C. difficile are treated differently depending on severity of disease. Mild and moderate cases may be treated with metronidazole while moderate-to-severe and relapsing cases are often treated with vancomycin or fidaxomicin. The presence of C. difficile disease is detected using a biomarker panel that includes C. difficile antigen (GDH), toxins A and B, and fecal lactoferrin. In patients suspected of C. difficile disease, if GDH is detected indicating the presence of C. difficile, and then toxins A and/or B are detected to indicate toxigenic C. difficile and support a diagnosis of C. difficile-associated disease, fecal lactoferrin concentrations are measured to determine severity of the disease by indicating the amount of intestinal inflammation.

Abstract: Cells are grown in 3D culture and topological features obtained by photomicrography are correlated to cell viability and cell cell interactions.

Abstract: A method for producing a seed of a plant resistant to seedling diseases is provided which includes contacting a non-pathogenic microbe corresponding to a seed-borne pathogen with a flower part of a host plant around the flowering time and collecting a seed of the host plant colonized by the non-pathogenic microbe obtained thereafter, and a method for controlling seedling diseases using the seed.

Abstract: The present invention provides a novel way to replenish the disc using retooled disc compositions to repair degenerative discs. There is no better source of proteoglycans than the actual disc material (6) itself. To this end, there has been developed a technique to remove the nucleus pulposus and retool the morphology of the nucleus pulposus to create a powder material (10) that is dry and can be stored at room temperature for long periods of time. This powder (10) can then be reconstituted with a variety of fluids, the most suitable being normal saline or lactated ringers to form a flowable mixture (20).

Abstract: The field of the invention is the analysis of target microorganisms in a complex sample. The present invention more particularly relates to a culture medium for the detection of at least one target microorganism including: at least one natural or synthetic fermentation or enzymatic activity substrate, and at least one selective agent, which inhibits non-target microorganisms, constituted by para-amino benzoic acid, one of its derivatives or one of their salts, at a concentration of between 0.05 and 1 g/L.

Abstract: The present invention relates to a device for detecting a fungal contamination in an internal environment, to the use thereof and also to a method for detecting a fungal contamination in an internal environment using such a device.

Abstract: The present disclosure provides a method for analyzing glyeart-derived monosaccharides in a sample. The present disclosure also provides a method for detecting or monitoring a disease or disorder in a patient. In addition, the present disclosure provides a method of determining aberrant glycotransferase activity. The present disclosure further provides a system for analyzing or comparing glycates in a sample.