Patents Examined by Richard G Hutson
  • Patent number: 10538746
    Abstract: The disclosure relates to polypeptides having carboxylic acid reductase (CAR) activity, including enzymes that catalyse the irreversible reduction of carboxylic acids, such as pimelic acid and adipic acid, to their respective semialdehydes. The enzymes have been engineered to have higher activity over a corresponding wild type enzyme. Provided herein are novel polypeptides and uses thereof related to the same.
    Type: Grant
    Filed: March 16, 2017
    Date of Patent: January 21, 2020
    Assignee: INVISTA North America S.à r.l.
    Inventors: Alexander Brett Foster, Adriana Leonora Botes
  • Patent number: 10533163
    Abstract: A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MC1 and a cytidine-specific recombinant RNase Cusativin.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: January 14, 2020
    Assignee: University of Cincinnati
    Inventors: Sarah Venus, Balasubrahmanyam Addepalli, Nicholas Paul Lesner, Patrick Alan Limbach
  • Patent number: 10533162
    Abstract: Provided are various novel DNA polymerases. Provided are: a DNA polymerase comprising: an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: January 14, 2020
    Assignee: KYUSHI UNIVERSITY, NATIONAL UNIVERSITY CORPORATION
    Inventors: Yoshizumi Ishino, Takeshi Yamagami, Hiroaki Matsukawa
  • Patent number: 10519428
    Abstract: Luciferases which are different from those known heretofore have been desired. A luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from the group consisting of valine at the position of 44, alanine at the position of 54 and tyrosine at the position of 138 is substituted with other amino acid(s) in the amino acid sequence of SEQ ID NO: 2.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: December 31, 2019
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Junichi Sato
  • Patent number: 10519431
    Abstract: A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: December 31, 2019
    Assignee: New England Biolabs, Inc.
    Inventors: Jennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis
  • Patent number: 10519203
    Abstract: A gene for biosynthesis of core structure of ophiobolin, the gene being the AuOS gene of Aspergillus sp. 094102, deposited with the accession number CCTCC No: M208153, the gene sequence thereof being shown as SEQ ID NO. 1. Also provided is a method of preparation of ophiobolin using the gene.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: December 31, 2019
    Assignee: WUHAN UNIVERSITY
    Inventors: Kui Hong, Huiying Meng, Hangzhen Chai, Ru Yin, Zixin Deng
  • Patent number: 10513671
    Abstract: Method for treating a hydrophobically-modified textile with an aqueous solution of a nuclease enzyme, preferably a deoxyribonuclease or ribonuclease enzyme, rinsing and drying the textile. Use of the finishing step for improved cleaning with an aqueous solution of a nuclease enzyme, preferably a deoxyribonuclease or ribonuclease enzyme.
    Type: Grant
    Filed: April 27, 2016
    Date of Patent: December 24, 2019
    Assignee: The Procter & Gamble Company
    Inventors: Neil Joseph Lant, Rebecca Louise Wood, Jeremie Robert Marcel Gummel
  • Patent number: 10508289
    Abstract: The present invention is in the field of genetic editing tools and methods of genetic engineering. It relates to the engineering of rare-cutting endonucleases designed to contract highly repetitive motives in chromosomes, which are at the origin of certain genetic diseases, in particular the so-called “triplet repeat diseases”, such as the Huntington disease. The invention encompasses the method for contracting the repetitive motives, the rare-cutting endonucleases for use to contract repetitive motives in a gene subjected to repeat disorder, the polynucleotides and vectors encoding thereof as well as the resulting pharmaceutical compositions.
    Type: Grant
    Filed: October 24, 2014
    Date of Patent: December 17, 2019
    Assignee: CELLECTIS
    Inventors: Philippe Duchateau, Alexandre Juillerat
  • Patent number: 10501507
    Abstract: The invention provides modified griffithsin polypeptides comprising the amino acid sequence of SEQ ID NO: 1, as well as corresponding nucleic acids, vectors, cells, fusion proteins, constructs, conjugates, and methods of inhibiting viral infection.
    Type: Grant
    Filed: February 10, 2016
    Date of Patent: December 10, 2019
    Assignees: The United States of America, as represented by the Secretary, Department of Health and Human Services, University of Louisville Research Foundation, Inc., University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Barry R. O'Keefe, Tinoush Moulaei, Kenneth E. Palmer, Lisa C. Rohan, Joshua L. Fuqua, Lindsay F. Kramzer
  • Patent number: 10501508
    Abstract: A fusion chimeric protein is described herein that can assemble a functional carboxysome core, which is able to fix carbon by taking atmospheric carbon dioxide and converting it into useful carbon-containing compounds such as 3-phosphoglycerate (3-PGA).
    Type: Grant
    Filed: August 24, 2017
    Date of Patent: December 10, 2019
    Assignee: Board of Trustees of Michigan State University
    Inventors: Cheryl A. Kerfeld, Cesar R. Gonzalez
  • Patent number: 10501813
    Abstract: Disclosed is a thermostable DNA polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of DNA for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of DNA collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention.
    Type: Grant
    Filed: August 28, 2017
    Date of Patent: December 10, 2019
    Assignees: HOKKAIDO MITSUI CHEMICALS INC., NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMA
    Inventors: Homare Tabata, Hiroshi Minami, Hideki Niimi, Isao Kitajima, Tomohiro Ueno, Shiroh Hayashi, Masashi Mori
  • Patent number: 10479981
    Abstract: The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: November 19, 2019
    Assignee: Novozymes A/S
    Inventors: Lars Henrik Oestergaard, Gernot J. Abel, Dorte Marie Koefoed Klitgaard, Annette Helle Johansen
  • Patent number: 10465219
    Abstract: The usefulness of ?-galactosidases derived from Bacillus circulans is further enhanced. A modified ?-galactosidase in which one or more amino acids selected from the group consisting of proline 182 (P182), tyrosine 187 (Y187), serine 188 (S188), tryptophan 405 (W405), alanine 406 (A406), glutamine 407 (Q407), tyrosine 449 (Y449), threonine 483 (T483), serine 512 (S512), serine 531 (S531), threonine 533 (T533), serine 534 (S534), asparagine 550 (N550), glutamine 551 (Q551), tryptophan 593 (W593), tyrosine 598 (Y598), proline 602 (P602), proline 604 (P604), tyrosine 609 (Y609), lysine 612 (K612), and tyrosine 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a ?-galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.
    Type: Grant
    Filed: September 26, 2014
    Date of Patent: November 5, 2019
    Assignee: AMANO ENZYME INC.
    Inventors: Genichi Nakamura, Yuko Kuritani, Satoru Ishihara, Tetsuya Takahashi
  • Patent number: 10457968
    Abstract: The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
    Type: Grant
    Filed: November 3, 2009
    Date of Patent: October 29, 2019
    Assignee: Kapa Biosystems, Inc.
    Inventors: William Bourn, Maryke Appel, Gavin Rush, John Foskett, Paul McEwan
  • Patent number: 10443048
    Abstract: A DNA polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: October 15, 2019
    Assignee: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Samir M. Hamdan, Masateru Takahashi
  • Patent number: 10435680
    Abstract: A thermostable cellobiohydrolase, having a cellulose-binding motif domain including (A1) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1, (B1) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having a cellulose-binding function, or (C1) a polypeptide including an amino acid sequence having 70% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having a cellulose-binding function, and also having a cellobiohydrolase catalytic domain, wherein the thermostable cellobiohydrolase exhibits hydrolysis activity against a substrate of phosphoric acid swollen Avicel at least under conditions of 95° C. and pH 5.5.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: October 8, 2019
    Assignee: Honda Motor Co., Ltd.
    Inventors: Jiro Okuma, Yoshitsugu Hirose, Migiwa Suda, Asuka Yamaguchi, Yasuhiro Kondo, Masaru Sato, Daisuke Shibata
  • Patent number: 10435675
    Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: October 8, 2019
    Assignee: Thermo Fisher Scientific Baltics UAB
    Inventors: Arunas Lagunavicius, Arvydas Janulaitis
  • Patent number: 10426821
    Abstract: A method of treating an inflammatory disease is disclosed. The method comprises administering to the subject a therapeutically effective amount of a polypeptide comprising a pro-domain of TNF-alpha converting enzyme (TACE), said polypeptide being devoid of a catalytic domain of said TACE, said polypeptide comprising a modification at a site selected from the group consisting of R58, R56 and K57 which renders said polypeptide resistant to furin degradation said polypeptide being capable of downregulating an activity of TACE, thereby treating the inflammatory disease.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: October 1, 2019
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Irit Sagi, Eitan Wong, Ran Afik
  • Patent number: 10412977
    Abstract: The present invention relates to methods and uses of at least one bacterial amylase in poultry feed to improve the nutritional value of the feed. The invention also relates to poultry feed and poultry feed additives comprising at least one bacterial amylase and at least one vitamin and/or mineral which improve the nutritional value of the feed.
    Type: Grant
    Filed: December 1, 2014
    Date of Patent: September 17, 2019
    Assignees: Novozymes A/S, DSM IP Assets B.V.
    Inventors: Inge Knap, Jose-Octavio Sorbara, Rafael Gustavo Hermes
  • Patent number: 10392637
    Abstract: The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: August 27, 2019
    Assignee: REG Life Sciences, LLC
    Inventors: Jason J. Lutes, Stephen B. Del Cardayre