Abstract: Cyanophage Syn5 RNA polymerase (RNAP) and methods of purifying it are provided. Cyanophage Syn5 RNAP having one or more promoter mutations are provided. Methods of expressing cyanophage Syn5 RNAP are provided. Novel promoter sequences and in vitro transcription systems utilizing Syn5 RNAP are provided. Methods of making high range ssRNA ladders are also provided. Methods of incorporating 2?-modified dNTPs using Syn5 RNA polymerase are provided.
Type:
Grant
Filed:
February 12, 2016
Date of Patent:
July 20, 2021
Assignee:
President and Fellows of Harvard College
Inventors:
Charles C. Richardson, Bin Zhu, Stanley Tabor
Abstract: A heat-inducible self-assembling fusion protein that includes a self-assembly domain and a target protein, wherein the self-assembly domain remains folded during assembly. The aggregate forming fusion protein can be induced to form protein aggregates conjugated to a target protein. The aggregates can be used similarly to beads in many laboratory protocols and other applications. Also disclosed are methods of making and using the protein aggregates.
Type:
Grant
Filed:
October 23, 2015
Date of Patent:
July 6, 2021
Assignee:
The University of Chicago
Inventors:
D. Allan Drummond, Joshua Riback, Jamie Scott, Alexandra Rojek, Pawel Laskowski, Ronald Rock, Jagoda Rokicka, Jakub Kucharczyk
Abstract: The present invention relates to methods for the conversion of the substrate specificity of desaturases. Specifically, the present invention pertains to a method for the conversion of the substrate specificity of a ?5 and/or ?6 desaturase to the substrate specificity of a ?4 desaturase, the method comprising: identifying regions and/or amino acid residues which control the substrate specificity of (i) the ?5 and/or ?6 desaturase and (ii) the ?4 desaturase; and replacing in the amino acid sequence of the mentioned ?5 and/or ?6 desaturase, the regions and/or amino acid residues which control the substrate specificity of the ?5 and/or ?6 desaturase, by the corresponding regions and/or amino acid residues which control the substrate specificity of the ?4 desaturase, thereby converting the substrate specificity of the ?5 and/or ?6 desaturase to the substrate specificity of the ?4 desaturase.
Abstract: A new CRISPR-associated (Cas) protein, termed “CasM,” is described, as well as polynucleotides encoding the same and methods of using CasM for site-specific genome engineering. CasM proteins are capable of targeting and cleaving single-stranded RNA.
Type:
Grant
Filed:
November 22, 2019
Date of Patent:
June 8, 2021
Assignee:
Locanabio, Inc.
Inventors:
Matthew Merrill Carter, Paul Daniel Donohoue
Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
Type:
Grant
Filed:
April 11, 2019
Date of Patent:
June 8, 2021
Assignee:
NUTRITION & BIOSCIENCES USA 4, INC.
Inventors:
Yougen Li, Mark S. Payne, Jared B. Parker, Slavko Kralj, Veli Alkan, Richard R. Bott, Robert Dicosimo, Qiong Cheng, Ellen D. Semke, Susan Marie Hennessey
Abstract: Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes, or that overexpress one or more heterologous transporters, leading to increased excretions of steviol glucosides of interest.
Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.
Type:
Grant
Filed:
September 6, 2018
Date of Patent:
May 18, 2021
Assignee:
Life Technologies Corporation
Inventors:
Joseph Beechem, Theo Nikiforov, Vi-En Choong, Xinzhan Peng, Guobin Luo, Cheng-Yao Chen, Michael Previte
Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.
Type:
Grant
Filed:
May 17, 2019
Date of Patent:
May 11, 2021
Assignee:
Life Technologies Corporation
Inventors:
Daniel Mazur, Peter Vander Horn, Eileen Tozer, Sihong Chen, Guobin Luo, Joshua Shirley, Kevin Heinemann
Abstract: Presented herein are methods and compositions for thermostable DNA polymerases that may be used to improve the PCR process and to improve the results obtained when using a thermostable DNA polymerase in other recombinant techniques such as DNA sequencing, nick-translation, and reverse transcription.
Abstract: The present invention provides, among other things, methods and compositions for large-scale production of capped mRNA using SUMO-Guanylyl Transferase fusion protein.
Type:
Grant
Filed:
October 19, 2018
Date of Patent:
May 4, 2021
Assignee:
Translate Bio, Inc.
Inventors:
Bettina Strack-Logue, Frank DeRosa, Michael Heartlein
Abstract: This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.
Type:
Grant
Filed:
December 21, 2016
Date of Patent:
April 20, 2021
Assignee:
INTEGRATED DNA TECHNOLOGIES, INC.
Inventors:
Mark Aaron Behlke, Joseph A. Walder, Richard Owczarzy, Scott D. Rose, Joseph R. Dobosy, Susan M. Rupp
Abstract: The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source.
Type:
Grant
Filed:
August 22, 2019
Date of Patent:
April 13, 2021
Assignee:
GENOMATICA, INC.
Inventors:
Jason J. Lutes, Stephen B. Del Cardayre
Abstract: The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
Type:
Grant
Filed:
April 15, 2016
Date of Patent:
April 13, 2021
Assignee:
Kapa Biosystems, Inc.
Inventors:
Wolfgang Schafer, Paul McEwan, Eric Van Der Walt, John Foskett, William Bourn
Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
Type:
Grant
Filed:
November 6, 2018
Date of Patent:
April 13, 2021
Assignee:
Pacific Biosciences of California, Inc.
Inventors:
Satwik Kamtekar, Lei Jia, Robin Emig, Erik Miller, Walter H. Lee
Abstract: Disclosed herein are methods and compositions for insertion of transgene sequences encoding proteins that is aberrantly expressed in disease or disorder such as a lysosomal storage disease.
Type:
Grant
Filed:
August 21, 2018
Date of Patent:
April 6, 2021
Assignee:
Sangamo Therapeutics, Inc.
Inventors:
Jeffrey C. Miller, David Paschon, Edward J. Rebar, Thomas Wechsler, Lei Zhang
Abstract: The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.
Type:
Grant
Filed:
May 3, 2018
Date of Patent:
March 30, 2021
Assignee:
Kapa Biosystems, Inc.
Inventors:
William Bourn, Maryke Appel, Gavin Rush, John Foskett, Paul J. McEwan
Abstract: Provided are compositions comprising recombinant polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for sequencing RNA or RNA/DNA templates. Polymerases that topologically encircle the template nucleic acid are provided. Also provided are methods of using such polymerases to make a DNA or to sequence a template comprising RNA.