Abstract: Chimeric proteins comprising a sequence nonspecific single-stranded nucleic-acid-binding domain joined to a catalytic nucleic-acid-modifying domain are provided. Methods comprising contacting a nucleic acid molecule with a chimeric protein, as well as systems comprising a nucleic acid molecule, a chimeric protein, and an aqueous solution are also provided. The joining of sequence nonspecific single-stranded nucleic-acid-binding domain and a catalytic nucleic-acid-modifying domain in chimeric proteins, among other things, may prevent the separation of the two domains due to their weak association and thereby enhances processivity while maintaining fidelity.
Abstract: DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.
Type:
Grant
Filed:
June 27, 2003
Date of Patent:
June 10, 2014
Assignee:
Metabolix, Inc.
Inventors:
Gjalt W. Huisman, Laura Z. Luo, Oliver P. Peoples
Abstract: Aspects of the invention provide engineered endonucleases that are characterized by both a long recognition sequence and specific cleavage outside of the recognition site. Engineered endonucleases of the invention are useful for manipulating long pieces of DNA.
Type:
Grant
Filed:
April 19, 2008
Date of Patent:
June 10, 2014
Assignee:
Celexion, LLC
Inventors:
Shaun Lippow, Dasa Lipovsek, Patricia M. Aha
Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Type:
Grant
Filed:
June 17, 2011
Date of Patent:
June 3, 2014
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Fred Reichert, Keith Bauer, Thomas W. Myers
Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. Such conjugates can exhibit enhanced biological activity and/or superior detectability as compared to conventional labeled polymerases. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.
Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Type:
Grant
Filed:
June 17, 2011
Date of Patent:
May 27, 2014
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Fred Reichert, Keith Bauer, Thomas W. Myers, Nancy J. Schoenbrunner, Joseph San Filippo
Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Type:
Grant
Filed:
June 17, 2011
Date of Patent:
May 27, 2014
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Fred Reichert, Keith Bauer, Thomas W. Myers
Abstract: DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.
Type:
Grant
Filed:
October 25, 2007
Date of Patent:
May 20, 2014
Assignee:
Metabolix, Inc.
Inventors:
Gjalt W. Huisman, Laura Z. Luo, Oliver P. Peoples
Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Type:
Grant
Filed:
June 17, 2011
Date of Patent:
May 13, 2014
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Fred Reichert, Keith Bauer, Thomas W. Myers
Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Type:
Grant
Filed:
June 17, 2011
Date of Patent:
May 13, 2014
Assignee:
Roche Medical Systems, Inc.
Inventors:
Fred Reichert, Keith Bauer, Thomas W. Myers
Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Type:
Grant
Filed:
June 17, 2011
Date of Patent:
May 13, 2014
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Fred Reichert, Keith Bauer, Thomas W. Meyer
Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Type:
Grant
Filed:
June 17, 2011
Date of Patent:
May 6, 2014
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Fred Reichert, Keith Bauer, Thomas W. Myers
Abstract: The invention relates to vWF cleaving entities having a molecular weight of 180 kD, 170 kD, 160 kD, 120 kD or 110 kD and an N-terminal amino acid sequence of AAGGILHLELLV, vWF cleaving complexes and methods for their production.
Type:
Grant
Filed:
July 11, 2008
Date of Patent:
April 22, 2014
Assignee:
Baxler Innovations GmbH
Inventors:
Bernhard Laemmle, Helena Elisabeth Schaller-Gerritsen, Miha Furlan, Peter Turecek, Hans-Peter Schwarz, Friedrich Scheiflinger, Gerhard Antoine, Randolf Kerschbaumer, Luigina Tagliavacca, Klaus Zimmermann
Abstract: The invention relates to vWF cleaving entities having a molecular weight of 180 kD, 170 kD, 160 kD, 120 kD or 110 kD and an N-terminal amino acid sequence of AAGGILHLELLV, vWF cleaving complexes and methods for their production.
Type:
Grant
Filed:
February 15, 2008
Date of Patent:
April 22, 2014
Assignee:
Baxter Innovations GmbH
Inventors:
Bernhard Laemmle, Helena Elisabeth Schaller-Gerritsen, Miha Furlan, Peter Turecek, Hans-Peter Schwarz, Friedrich Scheiflinger, Gerhard Antoine, Randolf Kerschbaumer, Luigina Tagliavacca, Klaus Zimmermann
Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
Type:
Grant
Filed:
August 31, 2012
Date of Patent:
April 22, 2014
Assignee:
Life Technologies Corporation
Inventors:
Marian Peris, Michael Phelan, Barnett Rosenblum, Stephen Hendricks
Abstract: A colorimetric reagent in the form of nanoparticles, composite nanoparticles, and nanoparticle coatings, including methods of use, methods of preparation, deposition, and assembly of related devices and specific applications. The colorimetric reagent comprises cerium oxide nanoparticles which are used in solution or immobilized on a solid support, either alone or in conjunction with oxidase enzymes, to form an active colorimetric component that reacts with an analyte to form a colored complex. The rate of color change and the intensity of the color are proportional to the amount of analyte present in the sample. Also described is the use of ceria and doped ceria nanoparticles as an oxygen storage/delivery vehicle for oxidase enzymes and applications in biocatalytic processes in anaerobic conditions of interest in biomedicine and bioanalysis.
Type:
Grant
Filed:
June 9, 2011
Date of Patent:
April 8, 2014
Assignee:
Clarkson University
Inventors:
Emanuela Silvana Andreescu, Maryna Ornatska, Cristina R. Ispas, Daniel Andreescu
Abstract: Specified restriction endonucleases have been characterized for the first time by their amino acid and DNA sequences. These sequences and those with at least 90% identity thereto have been used as probes in sequence similarity analyses to identify sequence matches in a sequence database that corresponds to novel restriction endonucleases or isoschizomers. The sequence similarity analyses includes selecting a positive sequence match from any sequence producing an expectation value of less than or equal to e?02.
Abstract: Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or pipeline maintenance.
Type:
Grant
Filed:
May 21, 2010
Date of Patent:
February 25, 2014
Assignee:
E I du Pont de Nemours and Company
Inventors:
Eric R. Choban, Robert D. Fallon, Edwin R. Hendrickson, Scott Christopoher Jackson, Abigail K. Luckring, Michael P. Perry, David Alan Suchanec, Sheryl M. Anzek
Abstract: Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as DNA polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized. It is demonstrated that chimeric nucleic acid polymerases of the present invention possess the chemical and physical properties of their component domains (e.g., exonuclease activity, thermostability) and that the chimeric polymerases are thermostable.
Type:
Grant
Filed:
August 8, 2002
Date of Patent:
January 28, 2014
Assignee:
Qiagen, GmbH
Inventors:
Dirk Löffert, Andreas Missel, Jie Kang