Abstract: A method of preparing simultaneously monoclonal antibodies specific for different cell-free products of activated human T lymphocytes is disclosed. Human T cells are activated in a medium supplemented with mouse serum rather than conventional calf serum. A supernatant prepared from the activated T cells is used to immunize mice. The dominant immunogens in the supernatant are the cell-free products of human T lymphocytes. The yield of hybrid cells which produce products reactive with cell-free products of human T lymphocytes is enhanced by injecting the immunized mice with a supernatant from mitogen-activated murine splenocytes. In addition, a novel radioimmunoadsorbent assay for screening hybrids to detect production of monoclonal antibodies reactive with cell-free products of human T lymphocytes is disclosed.

Abstract: A method for treating mastitis in ruminants by the administration of non-pathogenic Lactobacilli. The Lactobacilli are preferably administered in an oil emulsion which includes a water and an oil soluble emulsifier.

Abstract: A method for improving the absorption of pharmaceutical cations, in particular those of the 2A group and gold by orally administering the cations in combination with either yeast or Lactobacillus.

Abstract: A method for producing L-tryptophan by fermentation in high yields employing a microorganism of the genus Bacillus, which has been constructed by a gene splicing technique by incorporating a recombinant plasmid DNA containing an inserted DNA containing fragment controlling resistance to tryptophan-antagonists into a recipient strain of the genus Bacillus. The incorporated DNA fragment is a mutant of the genus Bacillus which was obtained from resistant to tryptophan-antagonists.

Abstract: An improved method for the purification of ribulose, 1,5-bisphosphate carboxylase (RuBisCO) comprises comminuting and homogenizing a plant material, such as leaves, in an aqueous solution. After fractionation to release the RuBisCO, sufficient polyethylene glycol (PEG) is added to cause crystallization of the RuBisCO. It has been found that treatment of the resulting PEG supernatant first by acidification to remove other proteins, and then by addition of a strong base to remove phosphorylated sugars, allows the recycling of the PEG in the process. Moreover, it is found that the phosphorylated sugars are a valuable by-product, suitable for example as a carbon source for the culture of microorganisms.

Abstract: Novel DNA constructs are provided for efficient expression of polypeptides by yeasts. The constructs employ yeast a-factor secretion leader and processing signals joined to a DNA sequence encoding a polypeptide of interest in reading frame with the a-factor signals. The constructions provide for the expression, secretion and maturation of the desired polypeptide. A strategy is provided for the isolation of the a-factor secretion leader and processing signals and the joining, by means of a relatively short adaptor, molecules of the DNA sequence encoding the polypeptide to the processing signals in proper reading frame.The bacterial cell strain E. coli HB101 (pAB163) was deposited at the A.T.C.C. on Apr. 20, 1983 and given Accession No. 39342.

Abstract: .alpha.-L-aspartylphenylalanine lower alkyl esters are prepared by a process wherein fumaric acid, ammonia and a lower alkyl ester of L-phenylalanine are contacted with a culture or treated culture of a microorganism belonging to the genus Pseudomonas, and being capable of producing a .alpha.-L-aspartyl-L-phenylalanine lower alkyl ester from fumaric acid, ammonia and a lower alkyl ester of L-phenylalanine.

Abstract: Monoclonal antibody reactive with SCC cells and unreactive with human neuroblastoma cells, human squamous cell carcinoma cells, and human large-cell undifferentiated lung carcinoma cells.

Abstract: Bacteriophage-resistant plant growth promoting rhizobacteria are employed for enhancing the yield of root crops, such as potatoes, sugar beets, radishes and the like, grown in soils which are infected by bacteriophage which limit the root colonization by the corresponding wild-type rhizobacteria.The strain SH5 PR3 was deposited at the ATCC for patent purposes on Jan. 20, 1983, and granted Accession No. 39270.

Abstract: The present invention relates to medicaments comprising, in association, at least one immunotoxin and chloroquin.

Abstract: A process for producing glutathione, involves cultivating a strain belonging to the genus Saccharomyces and having both an ability to produce glutathione and a resistance to 1,2,4-triazole or sodium azide in a culture medium accumulating glutathione in the microbial cells, harvesting the cells, and recovering the glutathione therefrom.

Abstract: An antibody of a human leukemia virus-related peptide obtained by collecting an antibody produced in a mammal body by administering to the mammal an antigen prepared by reacting a human leukemia virus-related peptide represented by formula (1):R-Pro-Val-Met-His-Pro-His-Gly-Ala-Pro-OH (1)whereinR is a hydrogen atom or a group shown by formula, H-Tyr-;as a hapten, with a carrier in the presence of a hapten-carrier binding agent.

Abstract: Enhanced immunogenicity is achieved by covalently linking immunogens to liposomes and injecting the membrane-bound-proteins into an appropriate vertebrate host. Methods and compositions are provided for producing antibodies.

Abstract: Methods and devices for assaying the sensitivity of of biopsied cells to therapeutic agents are disclosed. Cells are cultured in artificial organs and then contacted with a fluorogenic substrate such that living cells accumulate a characteristic amount of fluorescence. The agent is then introduced into the organ and changes in the fluorescence released by the cells serve as an indicator of the sensitivity of the cells to the agent.

Abstract: New product consisting of glycoprotein having a molecular weight of about 14,000 and an isoelectric point of about 4.6, its absorption spectrum having an absorption of .epsilon.=0.12 units of optical density (O.D.) at 280 nm for an aqueous solution of 1 mg (dry weight) per ml of water. This product has immunoregulating properties and is useful in allergic therapeutic composition.

Abstract: Hybridoma tumor cell line (ATCC Number CRL 8164). A monoclonal anti-erythropoietin antibody substance, Epoclonalan, produced by said cell line. Use of Epoclonalan in immonological methods for isolation of natural erythropoietin and for quantitative detection of erythropoietin in fluid samples.

Abstract: An immunoglobulin is provided which consists essentially of a mono-specific, hetero-molecular antibody which is mono-specific to a single antigenic or allergenic determinant. The antibody is specific to the H-epitope of a protein antigen or allergen. The H-epitope is defined by a sequence of at least six amino acids corresponding to the sequence of such amino acids in the protein antigen or allergen where the greatest local average hydrophilicity of the protein antigen or allergen is found.

Abstract: Artificial plant growth media suitable for suspension cell culture of sunflower plant cells are provided. Methods of using the media for growth of suspension cell culture of sunflower plant cells are provided. Methods for screening the oil content of sunflower seeds produced by sunflower plants utilizing the suspension cell growth characteristics of sunflower plant cells are provided.

Abstract: A method for the elaboration of large quantities of Bordetella pertussis protective antigens useful in the production of acellular vaccines for prevention of whooping cough.

Abstract: Immunological preparations are prepared by immunizing hens with an immunogen having a molecular or particle weight of at least 30,000, to a stage of hyper-immunization at which there occurs a plateau-like levelling-off of the antibody content of the serum. The immunogenicity of the immunogen can be enhanced by enlarging the immunogen particle mass. The eggs of the immunized hens are collected, the yolk is separated from the eggs, followed by separation of the lipid content of the yolk. The antibodies in the egg yolk are then rendered indispersable with the aid of a water-soluble linear filamentary non-charged polymer precipitant such as PEG and the indispersable antibodies are recovered. This precipitation of antibodies is advantageously preceded by a precipitation of caseinaceous proteins, lipid and yolk particles at lower polymer concentrations. Selected antibodies (IgY or IgG) can be concentrated by pH-controlled fractional precipitation with PEG.