Abstract: A mammalian blood protein-containing composition such as whole blood, plasma, serum, plasma concentrate, cryoprecipitate, cryosupernatant, plasma fractionation precipitate or plasma fractionation supernatant substantially free of hepatitis and other lipid coated viruses with the yield of protein activity to total protein being at least 80% is disclosed. The protein-containing composition is contacted with di- or trialkylphosphate, preferably a mixture of trialkylphosphate and detergent, usually followed by removal of the di- or trialkylphosphate.
Abstract: A medicament and method for inducing immunity in to infectious bovine keratoconjunctivitis in cattle. The medicament comprises the gram negative cocci Neisseria or Branhamella which are non-etiological agents of infectious Keratoconjunctivitis yet unexpectedly are found to afford an immunity to infectious bovine keratoconjunctivitis when administered to cattle.
Abstract: This invention provides an improved fermentation process for bioconversion of toluene to muconic acid.The process involves operating the bioconversion system under phosphate-limiting conditions so as to achieve an increase in specific muconic acid productivity with a stabilized population of microorganism such as an ATCC No. 31,916 type of Pseudomonas putida Biotype A mutant strain.
Type:
Grant
Filed:
January 13, 1983
Date of Patent:
August 13, 1985
Assignee:
Celanese Corporation
Inventors:
Jih-Han Hsieh, Sol J. Barer, Peter C. Maxwell
Abstract: A method and composition is provided to control fungal infection of seeds and seedlings. Certain species of Streptomyces are grown on dried ground shrimp waste preferably suspended in a mineral salts medium. The supernate derived from the culture provides fungal infection control when used as a seed-coating or seed-soaking material. The residue, consisting of unused shrimp waste and a Streptomyces species, also provides protection against fungal infection of plants such as cotton seedlings. The residue is employed as a amendment to the soil in the immediate area of the newly planted seed.
Abstract: Compositions containing therapeutically or immunologically active proteins are rendered substantially free from infectious agents such as viable viruses and bacteria without substantial loss of therapeutic or immunologic activity by mixing the protein composition with a complex formed from source of transition metal ions, such as copper ions, and an angularly-fused, polynuclear heterocyclic arene having two nitrogen atoms in a "cis-ortho" relationship, such as phenanthroline, and a reducing agent such as a thiol in amounts and at a temperature and for a time sufficient to inactivate substantially all of the viruses and bacteria contained therein. Compositions containing therapeutically active proteins substantially free from viral and bacterial infectivity, which have heretofore been unattainable, can be prepared by the method of the invention.
Abstract: A culture medium and method for the isolation and culture of plant protoplasts is disclosed which includes in it a quantity of neutral mammalian blood serum. An addition of 1 to 25% serum to protoplast media results in a greater yield of viable protoplast cells, helps to protect the protoplasts in culture, and makes it possible to obtain and maintain corn protoplasts, a heretofore difficult task.
Abstract: Entomopathogenic fungi useful in the control of insects is prepared by culturing mycelia in a suitable medium, harvesting the growing mycelia, treating the mycelia with a protective agent, drying the treated mycelia and grinding and storing the dried product.
Type:
Grant
Filed:
September 17, 1982
Date of Patent:
July 23, 1985
Assignee:
The United States of America as represented by the Secretary of Agriculture
Abstract: Monoclonal antibodies to theophylline having 5% or less cross-reactivity with caffeine and the continuous hybrid monoclonal cell lines for their production are provided. These antibodies are useful in a particle-enhanced turbidimetric inhibition immunoassay for theophylline.
Abstract: A non-lysozyme highly active bacteriolytic protein which is heat stable and has a relatively low molecular weight. The protein may be produced by immunizing an insect against E. coli and recovering the protein from the insect. The protein is useful for extracting proteins from genetically engineered bacteria and as a pharmaceutical for inhibiting certain bacteria.
Type:
Grant
Filed:
August 2, 1982
Date of Patent:
May 28, 1985
Assignee:
KabiGen AB
Inventors:
Dan Hultmark, Hakan Steiner, Torgny Rasmuson, Hans G. Boman
Abstract: A contraceptive agent is provided which comprises biologically-active alpha-lactalbumin. The agent can be administered to female mammals using a variety of pharmaceutically acceptable vehicles, provided the alpha-lactalbumin retains its biological activity in the presence of the vehicle.
Abstract: Protein PP.sub.13 is isolated from human placental tissues using a variety of methods, e.g., immunoadsorption. The protein has utility in production of an antiserum which has diagnostic applications. The placental specific proteins (e.g., PP.sub.13) are frequently detected in increasing amounts in women as pregnancy advances, and in patients with tumors especially of the tryphoblastic and embryonal types.
Abstract: An intravenous immune globulin preparation having at least 99% pure globulin protein and an anticomplement activity of less than 0.10 C'50 units/mg IgG prepared by: precipitating impurities from Cohn Fraction II in an aqueous-alcohol medium at defined temperature and pH, removing the precipitated impurities, stabilizing the diluted solution with albumin, concentrating the solution and removing the alcohol therefrom. Also prepared by this method, an intravenous, hyperimmune globulin preparation having increased antibody titers to sixteen serospecific strains of Pseudomonas aeruginosa.
Abstract: A method of preparing gamma globulin suitable for intravenous administration comprising a first step of treating a supernatant liquid separated from human blood plasma with ethanol under conditions of a pH of 5.5 to 6.5, an ethanol concentration of 30 to 35%, and a protein concentration of 1 to 2% by weight.