Patents Examined by Sally A Merkling
  • Patent number: 10293337
    Abstract: Apparatus for isolating an individual cell from a group of cells and transferring that isolated cell to a desired location, the apparatus comprising: a single-cell pipette tip, the single-cell pipette tip comprising: a structure having a distal end; a Y-shaped microchannel formed in the structure, the Y-shaped microchannel comprising a base microchannel, a first branch microchannel and a second branch microchannel, wherein the base microchannel extends to the distal end of the structure, the first branch microchannel is connectable to a first pressure channel, the second branch microchannel is connectable to a second pressure channel, and a single-cell trap is disposed in the base microchannel, distal to the convergence of the base microchannel with the first branch microchannel and the second branch microchannel.
    Type: Grant
    Filed: January 28, 2015
    Date of Patent: May 21, 2019
    Assignee: The Methodist Hospital
    Inventors: Lidong Qin, Kai Zhang
  • Patent number: 10288563
    Abstract: A plasmonic resonator can include: a substrate; a central resonator on the substrate; a first curved resonator disposed on the substrate and disposed at a first side of the central resonator; and a second curved resonator disposed on the substrate and disposed at a second side of the central resonator, the first curved resonator and the second curved resonator being asymmetric to each other with respect to the central resonator, and the central resonator and at least one of the first and second curved resonators being made of different materials.
    Type: Grant
    Filed: January 22, 2018
    Date of Patent: May 14, 2019
    Assignee: The Florida International University Board of Trustees
    Inventors: Arash Ahmadivand, Raju Sinha, Burak Gerislioglu, Nezih Pala
  • Patent number: 10272430
    Abstract: A disposable cartridge and method for measuring one or more properties of a blood sample are provided. The disposable cartridge can receive a sample when it is in an unsealed configuration. After adjusting the cartridge from the unsealed configuration to a sealed configuration, facilitated by a hingedly attached cap, pressurized air from an air bladder may be used to force the blood from a sample storage well into a detection chamber in a regulated manner, so that the one or more properties of the blood sample may then be measured.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: April 30, 2019
    Assignee: INVIDX CORP.
    Inventor: James Samsoondar
  • Patent number: 10261088
    Abstract: The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.
    Type: Grant
    Filed: October 7, 2015
    Date of Patent: April 16, 2019
    Assignees: California Institute of Technology, University of Southern California
    Inventors: Amir Goldkorn, Yu-Chong Tai, Tong Xu, Bo Lu
  • Patent number: 10233491
    Abstract: This disclosure provides, among other things, a cartridge comprising: (a) a cartridge body comprising a malleable material and having, disposed on a surface of the body, at least one valve body comprising a valve inlet and a valve outlet, each fluidically connected to a fluidic channel; and (b) a layer comprising a deformable material bonded to a surface of the cartridge body and sealing the at least one valve body at points of attachment, thereby forming at least one valve; wherein the at least one valve body is depressed in the cartridge body relative to the points of attachment and wherein the deformable material covering the at least one valve body retains sufficient elasticity after deformation such that in a ground state the valve is open.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: March 19, 2019
    Assignee: IntegenX, Inc.
    Inventors: Philip Justus Wunderle, Chungsoo Charles Park, David King, Dennis Lehto
  • Patent number: 10227633
    Abstract: The present disclosure provides a portable device for purifying biological sample. The device comprises a housing and a cartridge holder accommodated in the housing for holding at least one cartridge configured to purify the biological sample. At least one pump is positioned in the housing which is fluidly connectable to the cartridge to circulate the sample and one or more fluids through the cartridge. Further, at least one heating element is configured to heat a matrix chamber in the cartridge. At least one actuator is disposed in the housing to actuate one or more valves to selectively route the biological sample and the one or more fluids in the cartridge. A control unit is interfaced with the actuator, the heating element and the pump and is configured to regulate operation of the actuator, the pump and the heating element during purification of the biological sample.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: March 12, 2019
    Inventors: Manoj Mulakkapurath Narayanan, Chandrasekhar Bhaskaran Nair
  • Patent number: 10221451
    Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.
    Type: Grant
    Filed: June 29, 2015
    Date of Patent: March 5, 2019
    Inventors: Wolfgang Hinz, David Light, Todd Rearick, James A. Ball
  • Patent number: 10214772
    Abstract: A disposable test card configured to accept a fluid sample for an assay, and a method of manufacturing same, is disclosed herein. In a general example embodiment, a test card for analyzing a fluid sample includes a first substrate layer including an inlet port and an outlet port, a channel layer bonded to the first substrate layer, the channel layer including a microchannel placing the inlet port in fluid communication with the outlet port, and a second substrate layer bonded to the channel layer, the second substrate layer having electrodes printed adjacent to a target zone of the microchannel of the channel layer, wherein the electrodes are configured to raise the temperature of the fluid sample within the target zone of the microchannel when a current is applied thereto.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: February 26, 2019
    Assignee: FluxErgy, LLC
    Inventors: Ryan Alan Revilla, Roy James Heltsley, Steve Hoe Lee, Tej Rushikesh Patel
  • Patent number: 10208303
    Abstract: A solid-core ring-magnet having one or more cavities is provided. The magnet can have an overall cylindrical shape or a rectangular-prism shape. In either case, a portion of cavity walls of the magnet are ring shaped, causing the magnetic field lines to emanate from the magnet in the shape of a ring. The diameter of the ring shaped cavities can be constant throughout, constant through a portion of the cavity, variant throughout, or variant through a portion of the cavity. The cavities open to the end of the magnet, and terminate toward the core of the magnet. Also provided are systems and kits having solid-core ring-magnets. Methods of purifying a macromolecule using the solid-core ring-magnets are also provided.
    Type: Grant
    Filed: August 29, 2018
    Date of Patent: February 19, 2019
    Assignee: Alpaqua Engineering, LLC
    Inventor: Olaf Stelling
  • Patent number: 10191043
    Abstract: An assay test strip includes a flow path, a sample receiving zone, a label, a detection zone that includes a region of interest, and at least one position marker. The at least one position marker is aligned with respect to the region of interest such that location of the at least one position marker indicates a position of the region of interest. A diagnostic test system includes a reader that obtains light intensity measurement from exposed regions of the test strip, and a data analyzer that performs at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a control signal modifying at least one operational parameter of the reader based on at least one measurement obtained from the at least one reference feature.
    Type: Grant
    Filed: October 18, 2016
    Date of Patent: January 29, 2019
    Assignee: Alverix, Inc.
    Inventors: Patrick T. Petruno, John F. Petrilla, Michael J. Brosnan, Rong Zhou, Daniel B. Roitman, Bo U. Curry
  • Patent number: 10179332
    Abstract: A device for collecting cellular material from a fluid sample comprises first and second chambers that are releasably connected and which define a fluid flow path from the first chamber to the second chamber. A porous membrane is between the chambers when they are connected to each other, in the fluid flow path. An absorbent body is positioned in close proximity to the downstream surface of the porous membrane, and a resilient member is arranged to urge the absorbent body towards the porous membrane. In use, a sample introduced into the first chamber contacts the upstream surface of the porous membrane and fluid is drawn through the porous membrane and is absorbed by the absorbent body. Cellular material is retained upon the upstream surface of the porous membrane. After separation of the chambers, the porous membrane can be removed from the device for analysis of the deposited cellular material.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: January 15, 2019
    Assignee: Brightwake Limited
    Inventor: Stephen Cotton
  • Patent number: 10166009
    Abstract: A medical apparatus and method of preparing one or more cell blocks. The medical apparatus comprises at least one elongate tubular body having a proximal end and a distal end and a filter membrane disposed between the proximal end and a distal end of the elongate tubular body. The filter membrane, which can include alignment features and structural features to engage the tubular body, and/or cover, is sectionable. In other embodiments, a valve is provided for opening and closing fluid communication with the filter membrane.
    Type: Grant
    Filed: November 20, 2013
    Date of Patent: January 1, 2019
    Inventors: Anjali Saqi, Keith Yeager
  • Patent number: 10150118
    Abstract: A controlled transfer biological sample material collection device is disclosed which includes a body and a sample collection member for collecting the biological sample material, the body housing a sample storage medium for generally dry storage of the biological material, the collection member being moveable from an exposed position where collection of a biological sample is possible, to a transfer position which effects transfer of at least a portion of the collected sample to said medium. The body of the device slideably supports the sample collection member, and the body or collection member includes a ramp-like projection portion operable to force the collection member into the transfer position against the medium and to effect the transfer as the collection member slides within the body.
    Type: Grant
    Filed: May 14, 2014
    Date of Patent: December 11, 2018
    Assignee: GE Healthcare UK Limited
    Inventors: Peter James Tatnell, Stevan Paul Tortorella, Geraint Seymour, Cheryl Louise Potts, Alan Stuart Pierce, Samantha Jane Ogden, Neil John Williams
  • Patent number: 10150993
    Abstract: Provided herein is technology relating to depositing and/or placing a macromolecule at a desired site for an assay and particularly, but not exclusively, to methods and systems for placing or guiding a macromolecule such as a protein, a nucleic acid, or a protein: nucleic acid complex to an assay site, such as near a nanopore, a nanowell, or a zero mode waveguide.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: December 11, 2018
    Inventor: Mark A. Hayden
  • Patent number: 10118172
    Abstract: A fluid analysis cartridge includes a reference well including a macromolecular coloring reagent having an optical characteristic that varies according to a thickness of the reference well, and a test well including a test reagent having an optical characteristic that varies according to a concentration of a component of a fluid sample that reacts with the test reagent and a thickness of the test well.
    Type: Grant
    Filed: November 29, 2016
    Date of Patent: November 6, 2018
    Inventors: Jeo Young Shim, Jong Myeon Park, Jae Sung Lee, Do Gyoon Kim, Young Seop Seong, Yeong Bae Yeo, Hae Seok Lee, Kyu Youn Hwang
  • Patent number: 10112195
    Abstract: Provided is a flow cell for nucleic acid analysis used for a sequence reaction with photocleavable nucleotides where an efficiency of the photocleaving reaction can be enhanced and noise upon florescence detection can be mitigated, thereby improving the accuracy of sequencing, shortening a runtime, and extending a read length. The flow cell for nucleic acid analysis includes a first substrate 101 provided with an optical filter 102 reflecting first light for changing a chemical structure of a substance in a flow passage, a hollow sheet 103 having a hollow portion for forming the flow passage, and a second substrate 105 transmitting the first light, in which the first substrate, the hollow sheet, and the second substrate are attached to each other.
    Type: Grant
    Filed: June 11, 2014
    Date of Patent: October 30, 2018
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Masatoshi Narahara, Tomohiro Shoji
  • Patent number: 10114005
    Abstract: An object of the present invention is that variations in an applied membrane potential in the planar patch clamp device are suppressed to reduce a noise current, thereby enabling accurate measurement of an ion channel current. Disclosed is a planar patch clamp device including: an electrically insulative substrate provided with one or more fine through holes; a liquid reservoir that holds a conductive liquid provided on both surface sides of the through hole; and energizable electrode sections provided in the liquid reservoir; these electrode sections including: (a) an electrode vessel, at least part of which is made of an inorganic porous material, (b) an electrode in which a chloride NmCl layer is formed on the surface of a noble metal Nm, and (c) an electrode solution containing NmCl and an alkali metal chloride being dissolved therein at a saturated concentration.
    Type: Grant
    Filed: December 5, 2012
    Date of Patent: October 30, 2018
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Senthil Kumar Obuliraj, Yasutaka Nagaoka
  • Patent number: 10112194
    Abstract: A substrate for use in manufacture of a production master plate for production of a detection disc for carrying samples in an apparatus for detection of microscopic objects in a fluid, the substrate having a channel and separate focus structure, wherein the focus structure is a groove.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: October 30, 2018
    Assignee: Q-linea AB
    Inventors: Jonas Sven Peter Jarvius, Jan Grawe
  • Patent number: 10100356
    Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat.
    Type: Grant
    Filed: September 21, 2015
    Date of Patent: October 16, 2018
    Assignee: GENAPSYS, INC.
    Inventors: Hesaam Esfandyarpour, Mark F. Oldham, Kosar Baghbani Parizi, Eric S. Nordman
  • Patent number: 10093965
    Abstract: A system for preparing and analyzing a sample of biological material, including a test cartridge having a first housing defining a flow-through chamber, a second housing defining a central space within which the first housing is at least partially located. The first housing is rotatable relative to the second housing, and the second housing defines a plurality of circumferentially spaced chambers, one of the chambers having an inlet for receiving a sample, at least one of the chambers containing a liquid reagent, and at least one of the chambers comprising an analysis module, the chambers of the second housing each having an opening into the central space. The first housing has one or more openings into the central space so that openings can be selectively aligned with one of the openings into the chambers of the second housing by relative rotation of the first housing and second housings.
    Type: Grant
    Filed: September 25, 2017
    Date of Patent: October 9, 2018
    Inventors: Christofer Toumazou, Stuart Bhimsen Lowe, Steven William Green, Piers Sebastian Harding, Giles Hugo William Sanders, Nicholas James Wooder, Andreas Augustinus Werdich, Michiel Clemens Rene Twisk, Rene Heinz Joaquim Zander, Jonathan Casey, Hannah Victoria Hare, Richard Lintern, Stephanie Weichert, Steven James Wakefield, Kathrin Herbst, Luciano Zanchet