Patents Examined by Samuel W. Liu
  • Patent number: 11066682
    Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.
    Type: Grant
    Filed: April 2, 2012
    Date of Patent: July 20, 2021
    Assignee: Genomatica, Inc.
    Inventors: Derek L. Greenfield, Louis G. Hom, Fernando A. Sanchez-Riera, Zhihao Hu, Vikranth Arlagadda, Eli S. Groban, Scott A. Frykman
  • Patent number: 11066656
    Abstract: Modified PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and/or increased activity, are provided. Also provided are compositions and formulations and uses thereof.
    Type: Grant
    Filed: June 25, 2020
    Date of Patent: July 20, 2021
    Assignee: Halozyme, Inc.
    Inventors: Ge Wei, H. Michael Shepard, Qiping Zhao, Robert James Connor
  • Patent number: 11066444
    Abstract: Provided herein are peptide amphiphiles with binding affinity for the bone promoting growth factor BMP-2, and methods of use thereof. In particular nanofibers and gel scaffolds of BMP-2-binding peptide amphiphiles are provided.
    Type: Grant
    Filed: August 6, 2019
    Date of Patent: July 20, 2021
    Assignee: Northwestern University
    Inventors: Samuel I. Stupp, Erin L. Hsu, Wellington K. Hsu, Sungsoo Lee
  • Patent number: 11059865
    Abstract: Disclosed are catalytically active water-insoluble protein aggregates comprising fusion proteins which comprise a coiled-coil domain and a catalytic domain, methods of manufacturing such protein aggregates, and their use.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: July 13, 2021
    Assignee: evoxx technologies GmbH
    Inventors: Martina Pohl, Karl-Erich Jaeger, Martin Diener, Ulrich Krauss
  • Patent number: 11060080
    Abstract: A method for fermentation-production of a pentanediamine, comprising: culturing a cell expressing a lysine decarboxylase to obtain a whole cell fermentation broth comprising a pentanediamine; and extracting the pentanediamine from the whole cell fermentation broth, and striping the whole cell fermentation broth of carbon dioxide contained therein before adding a strong base. The method greatly increases a production volume of the pentanediamine.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: July 13, 2021
    Assignee: HEILONGJIANG EPPEN NEW MATERIALS CO., LTD.
    Inventors: Jiyin Ma, Tingyi Wen, Shuwen Liu, Yong Liang, Daihuan Li, Yun Zhang, Xiuling Shang, Chunguang Zhao, Xiaowei Guo, Gang Meng
  • Patent number: 11046945
    Abstract: The present invention relates to a labeled glutaminase (GLS) protein comprising a GLS protein and a fluorescent reporter group attached to the GLS protein, wherein the fluorescent reporter group is attached to the GLS protein within the glutaminase domain pfam04960 of GLS. The present invention also relates to isolated glutaminase protein mutants. Also disclosed is a method of screening for compounds that allosterically bind to a glutaminase protein. The present invention also relates to a method of identifying compounds that inhibit or stabilize tetramer formation of glutaminase protein. The present invention further relates to a screening kit for compounds that inhibit or stabilize tetramer formation.
    Type: Grant
    Filed: February 27, 2014
    Date of Patent: June 29, 2021
    Assignee: CORNELL UNIVERSITY
    Inventors: Clint A. Stalnecker, Jon W. Erickson, Sekar Ramachandran, Rick Cerione
  • Patent number: 11041149
    Abstract: Modified PH20 hyaluronidase polypeptides, including modified polypeptides that exhibit increased stability and/or increased activity, are provided. Also provided are compositions and formulations and uses thereof.
    Type: Grant
    Filed: March 19, 2020
    Date of Patent: June 22, 2021
    Assignee: Halozyme, Inc.
    Inventors: Ge Wei, H. Michael Shepard, Qiping Zhao, Robert James Connor
  • Patent number: 11034931
    Abstract: The invention relates to yeast cells with useful characteristics, including being capable of utilizing panose as sole carbon source and/or capable of utilizing one or more dipeptidesas sole nitrogen source. The invention also relates to yeast cells with useful genotypes including comprising at least 4 allelic genes encoding IMA1p and/or at least two allelic genes encoding IMA5p.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: June 15, 2021
    Assignee: Carlsberg Breweries A/S
    Inventors: Natalia Y. Solodovnikova, Jeppe Frank Andersen, Rosa Garcia Sanchez, Zoran Gojkovic
  • Patent number: 11015175
    Abstract: An object to be achieved by the present invention is to culture a filamentous fungus at a high density, thereby enabling mass production of a useful substance. The present invention provides a method of producing a substance, including the steps of: culturing a mutant filamentous fungus with no expression of ?-1,3-glucan to allow the filamentous fungus to produce a substance; and collecting the resulting substance.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: May 25, 2021
    Assignee: TOHOKU UNIVERSITY
    Inventors: Keietsu Abe, Katsuya Gomi, Kei Yoshimi
  • Patent number: 11015180
    Abstract: The present invention provides engineered carboxyesterase enzymes having improved properties as compared to a naturally occurring wild-type carboxyesterase enzymes, as well as polynucleotides encoding the engineered carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes in amidation reactions.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: May 25, 2021
    Assignee: Codexis, Inc.
    Inventors: Rama Voladri, David Entwistle, Christopher Michael Micklitsch, Vesna Mitchell, Nikki Dellas, Xiyun Zhang, Antoinette Sero, Brent Dorr, Douglas E. Fuerst
  • Patent number: 10996227
    Abstract: Pre-coated analysis substrates, and methods of making the substrates and using them to analyze animal tissue, are described. The pre-coated analysis substrates can be made by forming a matrix surface on an analysis substrate; adding a protease to the matrix surface to form a pre-coated analysis substrate; and placing an animal tissue specimen on the matrix surface. The animal tissue can then be analyzed by allowing the protease to partially digest the animal tissue specimen; and analyzing the partially digested animal tissue specimen by mass spectrometry.
    Type: Grant
    Filed: June 9, 2016
    Date of Patent: May 4, 2021
    Assignee: VANDERBILT UNIVERSITY
    Inventors: Richard Caprioli, Junhai Yang, Jeremy L. Norris, Faizan Zubair, Paul Edward Laibinis
  • Patent number: 10988741
    Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.
    Type: Grant
    Filed: May 6, 2019
    Date of Patent: April 27, 2021
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Yuiko Miura, Junichi Sato
  • Patent number: 10988738
    Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.
    Type: Grant
    Filed: February 15, 2019
    Date of Patent: April 27, 2021
    Assignees: Ikeda Food Research Co., Ltd., PHC Corporation
    Inventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
  • Patent number: 10961553
    Abstract: Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: March 30, 2021
    Assignee: Genomatica, Inc.
    Inventors: Zhihao Hu, Vikranth Arlagadda
  • Patent number: 10952970
    Abstract: The present invention includes compositions and methods for the use of an encapsulation additive having between about 0.1 to about 30 percent isolated and purified vitelline protein B to provide for mixed and extended release formulations.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: March 23, 2021
    Assignees: THE TEXAS A&M UNIVERSITY SYSTEM, THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Allison R. Ficht, Kenneth Carson, Cynthia Sheffield, John Herbert Waite
  • Patent number: 10927382
    Abstract: The present disclosure relates to the use of a switch for the production of heterologous non-catabolic compounds in microbial host cells. In one aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured under aerobic conditions followed by microaerobic conditions, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions. In another aspect, provided herein are genetically modified microorganisms that produce non-catabolic compounds more stably when serially cultured in the presence of maltose followed by the reduction or absence of maltose, and methods of producing non-catabolic compounds by culturing the genetically modified microbes under such culture conditions.
    Type: Grant
    Filed: August 7, 2013
    Date of Patent: February 23, 2021
    Assignees: AMYRIS, INC., TOTAL MARKETING SERVICES
    Inventors: Hanxiao Jiang, Adam Meadows
  • Patent number: 10926220
    Abstract: Methods for enzyme-enhanced CO2 capture include contacting a CO2-containing gas with an aqueous absorption solution at process conditions—such as high temperature, high pH, and/or using carbonate-based solutions—in the presence of Thermovibrio ammonificans carbonic anhydrase (TACA) or functional derivative thereof for catalyzing the hydration reaction of CO2 into bicarbonate and hydrogen ions and/or catalyzing the desorption reaction to produce a CO2 gas. The TACA may be provided to flow with the solution to cycle through a CO2 capture system that includes an absorber and a stripper.
    Type: Grant
    Filed: August 27, 2015
    Date of Patent: February 23, 2021
    Assignee: SAIPEM S.P.A.
    Inventors: Normand Voyer, Richard Daigle, Éric Madore, Sylvie Fradette
  • Patent number: 10927399
    Abstract: The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: February 23, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Ulrike Maria Mueller, Liang Wu, Lourina Madeleine Raamsdonk, Aaron Adriaan Winkler
  • Patent number: 10920255
    Abstract: A method is useful for the biocatalytic synthesis of proteinogenic L-amino acids, such as L-alanine, L-valine, L-methionine, L-leucine, L-isoleucine or L-phenylalanine from a respective aldehyde and carbon dioxide. In particular, the method is useful for the biocatalytic synthesis of L-methionine from 3-methylthio-propanal (“methional”) and carbon dioxide.
    Type: Grant
    Filed: November 30, 2017
    Date of Patent: February 16, 2021
    Assignee: Evonik Operations GmbH
    Inventors: Arne Skerra, Lukas Eisoldt
  • Patent number: 10888638
    Abstract: The present invention relates to a method of producing a collagen membrane that has particular mechanical properties.
    Type: Grant
    Filed: May 15, 2019
    Date of Patent: January 12, 2021
    Inventor: Ming Hao Zheng