Patents Examined by Sheridan Swope
  • Patent number: 10975335
    Abstract: Proteases that comprise an amino acid sequence that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length and that comprise, in the count in accordance with SEQ ID NO. 1, the amino acid substitution R99D in combination with at least two further amino acid substitutions that are selected from the group consisting of S3T, V4I, and V199I, display very good cleaning performance in particular on blood-containing stains, as well as very good temperature stability.
    Type: Grant
    Filed: October 22, 2016
    Date of Patent: April 13, 2021
    Assignee: Henkel AG & Co. KGaA
    Inventors: Hendrik Hellmuth, Marion Merkel, Brian Laufs, Susanne Wieland, Timothy O'Connell, Susanne Tondera, Thomas Weber
  • Patent number: 10975408
    Abstract: The present invention relates to cofactor regeneration systems, components and uses thereof and methods for generating and regenerating cofactors. The cofactor regeneration system comprises a first electron transfer component selected from a polypeptide comprising a NADH:acceptor oxido-reductase or NADPH:acceptor oxido-reductase, a second electron transfer component selected from a hydrogenase moiety and/or non-biological nanoparticles and an electronically conducting surface. The first and second electron transfer components are immobilised on the electrically conducting surface, and the first and second electron transfer components do not occur together in nature as an enzyme complex.
    Type: Grant
    Filed: February 19, 2018
    Date of Patent: April 13, 2021
    Assignees: OXFORD UNIVERSITY INNOVATION LIMITED, HUMBOLDT-UNIVERSITAT ZU BERLIN
    Inventors: Kylie Vincent, Lars Lauterbach, Oliver Lenz
  • Patent number: 10941371
    Abstract: The present disclosure relates to proteases that are variants of a Bacillus gibsonii protease, the proteases comprising an amino acid sequence which has at least about 70% sequence identity to the amino acid sequence given in SEQ ID No. 1 over its entire length, and which has an amino acid substitution on at least one of the positions corresponding to the positions 12, 43, 122, 127, 154, 156, 160, 211 or 222, relating in each case to the numbering according to SEQ ID No. 1. The present disclosure also relates to the production and use thereof. Said type of proteases have a very good cleaning performance.
    Type: Grant
    Filed: June 1, 2017
    Date of Patent: March 9, 2021
    Assignee: Henkel AG & Co. KGaA
    Inventors: Daniela Herbst, Timothy O'Connell, Nina Mussmann, Inga Kerstin Vockenroth, Thomas Weber
  • Patent number: 10935546
    Abstract: The present invention deals with heterotrimeric AMP-activated protein kinase (AMPK) comprising a fluorophore pair wherein the conformational change can be measured by FRET. It represents an advanced tool to screen and identify AMPK interactors in vitro and in cells in vivo. Such invention can also be considered as a reporter of the cellular energy status as it allows the spatiotemporal monitoring, in situ, of fluctuations in the ratio of AMP and ADP versus ATP.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: March 2, 2021
    Assignees: Universite Grenoble Alpes, European Molecular Biology Laboratory
    Inventors: Imre Berger, Martin Pelosse, Uwe Schlattner
  • Patent number: 10928381
    Abstract: Provided herein are compositions and systems comprising engineered cells useful for the measurement of autophagy induction, maturation and flux in a high-throughput manner. In particular, provided herein are human embryonic kidney (HEK) cells that express the native autophagy substrate and marker protein LC3 fused to Dendra2, a photoconvertable fluorescent protein; this manipulation allows assessment of all three stages of autophagy (e.g., induction, maturation, and cargo degradation), noninvasively in living cells, without the need for protein overexpression that may interfere with autophagy activity.
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: February 23, 2021
    Assignee: The Regents of the University of Michigan
    Inventors: Sami Barmada, Nathaniel Safren, Elizabeth Tank
  • Patent number: 10927361
    Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: July 14, 2017
    Date of Patent: February 23, 2021
    Assignee: NOVOZYMES A/S
    Inventors: Keiichi Ayabe, Tomoko Matsui, Aki Tomiki, Yuma Kurakata, Esben P. Friis, Jens E. Nielsen, Roland Alexander Pache
  • Patent number: 10927364
    Abstract: The present invention relates to polypeptides having carbonic anhydrase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: July 20, 2017
    Date of Patent: February 23, 2021
    Assignee: Novozymes A/S
    Inventors: Sonja Salmon, Martin Simon Borchert, Thomas Holberg Blicher, Wolfgang Streit, Mirjam Perner
  • Patent number: 10920253
    Abstract: Described is a method for the enzymatic production of acetyl phosphate from formaldehyde using a phosphoketolase or a sulfoacetaldehyde acetyltransferase.
    Type: Grant
    Filed: March 13, 2015
    Date of Patent: February 16, 2021
    Assignee: Scientist of Fortune, S.A.
    Inventor: Philippe Marliere
  • Patent number: 10920209
    Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
    Type: Grant
    Filed: February 13, 2020
    Date of Patent: February 16, 2021
    Assignee: NOVOZYMES A/S
    Inventors: Frank Winther Rasmussen, Peter Kamp Hansen, Lars Lehman Hylling Christensen
  • Patent number: 10899823
    Abstract: Some embodiments of the systems, methods and compositions provided herein relate to a compound protease. In some embodiments, the compound protease includes a protease domain and a cut site for another enzyme. In some embodiments, the compound protease includes an association domain. In some embodiments, the compound protease is part of a protein circuit.
    Type: Grant
    Filed: January 17, 2019
    Date of Patent: January 26, 2021
    Assignee: California Institute of Technology
    Inventors: Xiaojing Gao, Lucy S. Chong, Michael Elowitz, Mark William Budde, Matthew Sun-min Kim
  • Patent number: 10900000
    Abstract: This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN? subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: January 26, 2021
    Assignee: The Procter & Gamble Company
    Inventors: Philip Frank Souter, Glenn Steven Ward, Viktor Yuryevich Alekseyev, Joshua Roy Basler, Luis Gustavo Cascao-Pereira, David A Estell, Ayrookaran J Poulose, James T Kellis, Jr., Alexander Pisarchik, Daniel Torres-Pazmino
  • Patent number: 10889785
    Abstract: Formulations, compositions and products for reducing allergenic Fel d 1 from the environment are disclosed. The formulations include at least one protease that interacts with the Fel d 1 and substantially degrades allergenic epitopes on the Fel d 1. Methods of using the formulations for reducing or eliminating allergenic Fel d 1 from the environment are also disclosed.
    Type: Grant
    Filed: April 4, 2018
    Date of Patent: January 12, 2021
    Assignee: SOCIÉTÉ DES PRODUITS NESTLÉ S.A.
    Inventors: George Wells, Delphine Tissot-Favre, Ebenezer Satyaraj, Juergen Eck, Daniel Meyer, Torsten Ertongur-Fauth, Alexander Pelzer, Scott Sherrill, Peichuan Sun
  • Patent number: 10865401
    Abstract: The present disclosure relates to proteases comprising an amino acid sequence which has at least about 70% sequence identity to the amino acid sequence indicated in SEQ ID NO. 1, over the entire length thereof, and in which the amino acids in the positions corresponding to positions 24, 33, 53, 78, 101, 128 and 217 according to SEQ ID NO. 1 are substituted at 24G, 33T, 53G, 78N, 101N, 128A and 217Q, and to the production and use thereof. Said type of proteases have a very good cleaning performance compared to reference proteases.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: December 15, 2020
    Assignee: Henkel AG & Co. KGaA
    Inventors: Daniela Herbst, Timothy O'Connell, Nina Mussmann, Brian Laufs, Ayhan Aydemir, Renee Charlott Eichstaedt, Inken Prueser
  • Patent number: 10865392
    Abstract: The present invention relates to acetyl transferases, nucleic acid sequences coding therefore, expression constructs and vectors comprising these sequences, microorganisms transformed therewith and use thereof.
    Type: Grant
    Filed: October 4, 2019
    Date of Patent: December 15, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Christopher Farrell, Peter Houston, Lisa Laprade, Nathalie Balch, Maria Mayorga
  • Patent number: 10858679
    Abstract: Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: December 8, 2020
    Assignee: EVONIK DEGUSSA GMBH
    Inventors: Rainer Figge, Philippe Soucaille, Guillaume Barbier, Gwénaëlle Bestel-Corre, Cédric Boisart, Michel Chateau
  • Patent number: 10815464
    Abstract: Disclosed is a modified microorganism producing putrescine or ornithine, and a method for producing putrescine or ornithine using the same.
    Type: Grant
    Filed: July 10, 2019
    Date of Patent: October 27, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Su Jin Park, Young Lyeol Yang, Hye Won Um, Hong Xian Li, Kyoung Min Lee, Baek Seok Lee, Hyo Hyoung Lee, Hee Kyoung Jung
  • Patent number: 10801052
    Abstract: The present invention relates to cofactor regeneration systems, components and uses thereof and methods for generating and regenerating cofactors. The cofactor regeneration system comprises a first electron transfer component selected from a polypeptide comprising a NADH:acceptor oxido-reductase or NADPH:acceptor oxido-reductase, a second electron transfer component selected from a hydrogenase moiety and/or non-biological nanoparticles and an electronically conducting surface. The first and second electron transfer components are immobilised on the electrically conducting surface, and the first and second electron transfer components do not occur together in nature as an enzyme complex.
    Type: Grant
    Filed: October 3, 2012
    Date of Patent: October 13, 2020
    Assignees: OXFORD UNIVERSITY INNOVATION LIMITED, HUMBOLDT-UNIVERSITAT ZU BERLIN
    Inventors: Kylie Vincent, Lars Lauterbach, Oliver Lenz
  • Patent number: 10788492
    Abstract: The present application demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.
    Type: Grant
    Filed: January 15, 2015
    Date of Patent: September 29, 2020
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Thomas Shenk, Todd M. Greco, Ileana M. Cristea, Rommel A. Mathias, Adam Oberstein
  • Patent number: 10787696
    Abstract: A system for the identification of proteases and protease inhibitors is provided. The system has at least two components. The first component is a reporter construct with at least one binding site, a transcriptional promoter, an inducible promoter region, and at least one reporter gene, all functionally connected for expression of the reporter gene(s) in functional coordination with a transcriptional activation agent. The second component is a transcriptional activation agent comprising a nucleic acid binding domain, at least one protease substrate domain, and at least one transcriptional activation domain for an inducible promoter. The system allows detection and evaluation of agents affecting protease activity directed to the protease substrate domain. The system also allows for the detection of the presence of proteases in environmental samples.
    Type: Grant
    Filed: September 13, 2013
    Date of Patent: September 29, 2020
    Assignee: IPSEN PHARMA S.A.S.
    Inventors: George A. Oyler, Yung-Nien Chang, Yien Che Tsai
  • Patent number: 10779552
    Abstract: A liquid or dried granulated milk clotting aspartic protease enzyme composition and process for isolating a milk clotting aspartic protease enzyme of interest.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: September 22, 2020
    Assignee: CHR. HANSEN A/S
    Inventors: Martin Lund, Jonas Jacobsen, Johannes Maarten Van Den Brink