Patents Examined by Stephen Kapushoc
  • Patent number: 8329397
    Abstract: This invention relates to the detection and quantitation of target nucleic acids in a heterogeneous mixture in a Sample and the methods of use thereof. The detection system includes a chemiluminescent molecule, a chemiluminescent substrate, a dye that is light responsive when intercalated into nucleic acids and nucleic acids. This invention is useful in any application where detection of a specific nucleic acid sequence is desirable, or where the detection of enzymes that modify nucleic acids is desirable such as diagnostics, research uses and industrial applications.
    Type: Grant
    Filed: June 5, 2006
    Date of Patent: December 11, 2012
    Assignee: Beacon Biotechnology Inc.
    Inventors: Anthony West, Millard Gambrell Cull
  • Patent number: 8323887
    Abstract: The present invention provides a method for combining a fluid delivery system with an analysis system for performing immunological or other chemical of biological assays. The method comprises a miniature plastic fluidic cartridge containing a reaction chamber with a plurality of immobilized species, a capillary channel, and a pump structure along with an external linear actuator corresponding to the pump structure to provide force for the fluid delivery. The plastic fluidic cartridge can be configured in a variety of ways to affect the performance and complexity of the assay performed.
    Type: Grant
    Filed: August 16, 2006
    Date of Patent: December 4, 2012
    Inventors: James Russell Webster, Ping Chang, Shaw-Tzuv Wang, Chi-Chen Chen, Rong-I Hong
  • Patent number: 8318460
    Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.
    Type: Grant
    Filed: February 11, 2010
    Date of Patent: November 27, 2012
    Assignee: Trustees of Boston University
    Inventors: Charles R Cantor, Chunming Ding
  • Patent number: 8313905
    Abstract: A detection oligomer and method for controlling the quality of a biochip using the detection oligomer are provided. The detection oligomer includes a template having a first and second end and having a sequence that is complementary to a specific sequence of an oligomer, and a hairpin connected to the first end of the template.
    Type: Grant
    Filed: January 23, 2009
    Date of Patent: November 20, 2012
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Joo-Won Rhee, Byung-chul Kim, Sun-Ok Jung, Seung-Hei Cho
  • Patent number: 8313904
    Abstract: Characteristics of a chemical or biological sample are detected using an approach involving light detection. According to an example embodiment of the present invention, an assaying arrangement including a light detector is adapted to detect light from a sample, such as a biological material. A signal corresponding to the detected light is used to characterize the sample, for example, by detecting a light-related property thereof. In one implementation, the assaying arrangement includes integrated circuitry having a light detector and a programmable processor, with the light detector generating a signal corresponding to the light and sending the signal to the processor. The processor provides an output corresponding to the signal and indicative of a characteristic of the sample.
    Type: Grant
    Filed: November 26, 2008
    Date of Patent: November 20, 2012
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Abbas El Gamal, Helmy Eltoukhy, Khaled Salama
  • Patent number: 8309699
    Abstract: This invention relates to transgenic winter oilseed rape (WOSR) plants, plant material and seeds, harboring a specific transformation event. It pertains to winter oilseed rape plants, more particularly to a pair of winter oilseed rape plants, which is particularly suited for the production of hybrid seed. More specifically, one plant is characterized by being male-sterile, due to the presence in its genome of a male sterility gene, while the other is characterized by carrying a fertility-restorer gene, capable of preventing the activity of the male-sterility gene. The invention further provides a method for producing hybrid seed, a process for producing a transgenic WOSR plant oil or plant, and a method to identify a transgenic plant, cell or tissue. A kit for identifying the transgenic plants comparing the elite event of the present invention is also described.
    Type: Grant
    Filed: August 17, 2011
    Date of Patent: November 13, 2012
    Assignee: Bayer Cropscience NV
    Inventors: Greta De Both, Marc De Beuckeleer
  • Patent number: 8309307
    Abstract: The present invention generally relates to spatial and structural genomic analysis compositions, which can be used for the mapping of chromosomes and structural analyses of chromosomal rearrangements, including the entire chromosome, as well as specific portions or regions of interest of the chromosomes. In some embodiments, multiple portions of the genome can be distinguished, for instance, using a first detection entity and a second detection entity different from the first detection entity. The detection entities may be immobilized relative to oligonucleotides, which may be selected to bind to different locations within the chromosome. For instance, the oligonucleotides may be at least substantially complementary to the chromosome, e.g., substantially complementary to a specific location of the chromosome.
    Type: Grant
    Filed: November 1, 2011
    Date of Patent: November 13, 2012
    Assignee: Agilent Technologies, Inc.
    Inventors: Michael Thomas Barrett, Michael P. Caren
  • Patent number: 8298767
    Abstract: Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a “reflex method”. In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided.
    Type: Grant
    Filed: August 13, 2010
    Date of Patent: October 30, 2012
    Assignee: Population Genetics Technologies Ltd
    Inventors: Sydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
  • Patent number: 8288096
    Abstract: A method for the diagnosis of SMEI in a patient comprising: (1) detecting an alteration in the SCN1A gene, including in a regulatory region of the gene, in a patient sample; (2) ascertaining whether the alteration is known to be SMEI associated or non-SMEI associated; and (3) (a) establishing a diagnosis of a high probability of SMEI where the alteration is known to be SMEI associated; or (b) establishing a diagnosis of a low probability of SMEI where the alteration is non-SMEI associated; or (e) or, if not known to be either, (i) considering genetic data for parents and/or relatives; (ii) establishing whether the alteration has arisen de novo or is inherited; and (iii) establishing a diagnosis of a low probability of SMEI where the alteration is inherited but a diagnosis of a high probability of SMEI if the alteration is de novo.
    Type: Grant
    Filed: April 26, 2010
    Date of Patent: October 16, 2012
    Assignee: Bionomics Limited
    Inventors: Steven Petrou, Samuel Frank Berkovic, Ingrid Eileen Scheffer
  • Patent number: 8278429
    Abstract: The present invention relates generally to the field of diagnostic and detection assays. More particularly, the present invention provides methods and reagents including biochips for detecting the presence of, or distinguishing between, one or more analytes in a sample.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: October 2, 2012
    Assignee: Genera Biosystems Limited
    Inventors: Daniel J. Park, Zaheer Khan, Karl F. Poetter
  • Patent number: 8263334
    Abstract: According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.
    Type: Grant
    Filed: March 18, 2011
    Date of Patent: September 11, 2012
    Assignee: Hitachi Plant Technologies, Ltd.
    Inventors: Hajime Ikuta, Kiyokazu Takemura
  • Patent number: 8221974
    Abstract: According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time.
    Type: Grant
    Filed: October 29, 2008
    Date of Patent: July 17, 2012
    Assignee: Hitachi Plant Technologies, Ltd.
    Inventors: Hajime Ikuta, Kiyokazu Takemura
  • Patent number: 8216783
    Abstract: This disclosure provides tyrosine kinase protein and nucleic acid variants, particularly FGFR4 variants, which are linked to increased risk of tumor metastasis. The disclosure further provides methods of diagnosis and prognosis, and development of new therapeutic agents using these molecules and fragments thereof, and kits for employing these methods and compositions.
    Type: Grant
    Filed: April 14, 2009
    Date of Patent: July 10, 2012
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Javed Khan, James G. Taylor, VI, Tai Cheuk
  • Patent number: 8206906
    Abstract: The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
    Type: Grant
    Filed: September 5, 2008
    Date of Patent: June 26, 2012
    Assignee: BASF SE
    Inventors: Chengyan Zhao, Robert Ascenzi, Bijay K. Singh
  • Patent number: 8192932
    Abstract: The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5? end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5? end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.
    Type: Grant
    Filed: February 12, 2010
    Date of Patent: June 5, 2012
    Assignees: Sloan-Kettering Institute For Cancer Research, Invitrogen Corporation
    Inventors: Stewart Shuman, JoAnn Sekiguchi, John Comiskey, Joseph Fernandez, James Hoeffler, Robert Marcil
  • Patent number: 8178294
    Abstract: Disclosed is a method for determining haplotypes useful for large-scale genetic analysis, within a genomic reference sequence of interest, for a human subpopulation. The method can applied to statistically evaluating the genotypes of subjects for any statistically significant association with a phenotype of interest, such as insulin resistance or coronary artery disease. Thus, also disclosed are a method of detecting a genetic predisposition in a human subject for certain biological conditions, which may be related to coronary artery disease.
    Type: Grant
    Filed: November 28, 2006
    Date of Patent: May 15, 2012
    Assignee: Cedars-Sinai Medical Center
    Inventors: Kent D. Taylor, Jerome I. Rotter, Huiying Yang, Willa A. Hsueh, Xiuqing Guo, Leslie J. Raffel, Mark O. Goodarzi, Yii-Der Ida Chen
  • Patent number: 8168229
    Abstract: A method of making a pliable, bioabsorbable hemostatic dressing wherein the dressing is composed of fibers with at least one molecular-scale coating, which upon first contact with blood and due to a large area of contact with blood per unit weight of active ingredients, initiates and accelerates the biochemical blood clotting cascade processes.
    Type: Grant
    Filed: September 27, 2006
    Date of Patent: May 1, 2012
    Assignee: LNK Chemsolutions, LLC
    Inventors: Gustavo Larsen, Ruben Spretz
  • Patent number: 8163486
    Abstract: A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof.
    Type: Grant
    Filed: November 17, 2010
    Date of Patent: April 24, 2012
    Assignee: Canon Kabushiki Kaisha
    Inventors: Nobuhiro Tomatsu, Toshifumi Fukui, Nobuyoshi Shimizu, Atsushi Takayanagi
  • Patent number: 8158766
    Abstract: The present invention relates to a probe for selecting ES cells, which characteristically contains one of DNAs having base sequences depicted in SEQ ID Nos; 1, 2, 3, 4, 5, 6, 7 and 8, or DNAs having base sequences depicted in SEQ ID Nos; 9, 11, 13, 15, 17, 19, 21, 23 and 41 and a screening method of ES cell using this probe. Preparation of a probe for selecting ES cells becomes feasible by identifying plural gene with ES cell-specific expressions (ECAT genes) and using the information of the base sequences of these gene groups. Efficient selection of ES cell enables supply of a large amount of ES cell expected to be applicable to regenerative medicine.
    Type: Grant
    Filed: July 10, 2006
    Date of Patent: April 17, 2012
    Assignees: Dainippon Sumitomo Pharma Co., Ltd.
    Inventors: Shinya Yamanaka, Eiko Kaiho
  • Patent number: 8153371
    Abstract: A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof.
    Type: Grant
    Filed: November 17, 2010
    Date of Patent: April 10, 2012
    Assignee: Canon Kabushiki Kaisha
    Inventors: Nobuhiro Tomatsu, Toshifumi Fukui, Nobuyoshi Shimizu, Atsushi Takayanagi